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ZA20D3 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  ZFAND6
Gene Name:  zinc finger, AN1-type domain 6
Sequence             
Original Clone ID: FLH268492.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329751

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 627nts         Open reading frame : 1 to 627
Reference Sequence Alignment

ATGGCTCAAGAAACTAATCACAGCCAAGTGCCTATGCTTTGTTCCACTGGCTGTGGATTT
TATGGAAACCCTCGTACAAATGGCATGTGTTCAGTATGCTATAAAGAACATCTTCAAAGA
CAGAATAGTAGTAATGGTAGAATAAGCCCACCTGCAACCTCTGTCAGTAGTCTGTCTGAA
TCTTTACCAGTTCAATGCACAGATGGCAGTGTGCCAGAAGCCCAGTCAGCATTAGACTCT
ACATCTTCATCTATGCAGCCCAGCCCTGTATCAAATCAGTCACTTTTATCAGAATCTGTA
GCATCTTCTCAATTGGACAGTACATCTGTGGACAAAGCAGTACCTGAAACAGAAGATGTG
CAGGCTTCAGTATCAGACACAGCACAGCAGCCATCTGAAGAGCAAAGCAAGTCTCTTGAA
AAACCGAAACAAAAAAAGAATCGCTGTTTCATGTGCAGGAAGAAAGTGGGACTTACTGGG
TTTGAATGCCGGTGTGGAAATGTTTACTGTGGTGTACACCGTTACTCAGATGTACACAAT
TGCTCTTACAATTACAAAGCCGATGCTGCTGAGAAAATCAGAAAAGAAAATCCAGTAGTT
GTTGGTGAAAAGATCCAAAAGATTTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 938
Start on reference sequence 312
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ZFAND6
Symbol Nomenclature : ZFAND6
SYNONYM : AWP1; ZA20D3; ZFAND5B
Designation : AN1-type zinc finger protein 6|protein associated with PRK1|zinc finger, A20 domain containing 3
Full Nomenclature : zinc finger, AN1-type domain 6
GENEID : 54469
Locus Tag : HT032
HGNC : 30164
MIM : 610183
Vega : OTTHUMG00000144169
Target GenBank: BC005283

 

Reference Sequence Alignment

  

HsCD00329751        ATGGCTCAAGAAACTAATCACAGCCAAGTGCCTATGCTTTGTTCCACTGGCTGTGGATTT
NM_001242913.1      ATGGCTCAAGAAACTAATCACAGCCAAGTGCCTATGCTTTGTTCCACTGGCTGTGGATTT
NM_001242916.1      ATGGCTCAAGAAACTAATCACAGCCAAGTGCCTATGCTTTGTTCCACTGGCTGTGGATTT
NM_019006.3         ATGGCTCAAGAAACTAATCACAGCCAAGTGCCTATGCTTTGTTCCACTGGCTGTGGATTT
NM_001242915.1      ATGGCTCAAGAAACTAATCACAGCCAAGTGCCTATGCTTTGTTCCACTGGCTGTGGATTT
NM_001242914.1      ATGGCTCAAGAAACTAATCACAGCCAAGTGCCTATGCTTTGTTCCACTGGCTGTGGATTT
NM_001242911.1      ATGGCTCAAGAAACTAATCACAGCCAAGTGCCTATGCTTTGTTCCACTGGCTGTGGATTT
NM_001242912.1      ATGGCTCAAGAAACTAATCACAGCCAAGTGCCTATGCTTTGTTCCACTGGCTGTGGATTT
NM_001242917.1      ATGGCTCAAGAAACTAATCACAGCCAAGTGCCTATGCTTTGTTCCACTGGCTGTGGATTT
                    ************************************************************

HsCD00329751        TATGGAAACCCTCGTACAAATGGCATGTGTTCAGTATGCTATAAAGAACATCTTCAAAGA
NM_001242913.1      TATGGAAACCCTCGTACAAATGGCATGTGTTCAGTATGCTATAAAGAACATCTTCAAAGA
NM_001242916.1      TATGGAAACCCTCGTACAAATGGCATGTGTTCAGTATGCTATAAAGAACATCTTCAAAGA
NM_019006.3         TATGGAAACCCTCGTACAAATGGCATGTGTTCAGTATGCTATAAAGAACATCTTCAAAGA
NM_001242915.1      TATGGAAACCCTCGTACAAATGGCATGTGTTCAGTATGCTATAAAGAACATCTTCAAAGA
NM_001242914.1      TATGGAAACCCTCGTACAAATGGCATGTGTTCAGTATGCTATAAAGAACATCTTCAAAGA
NM_001242911.1      TATGGAAACCCTCGTACAAATGGCATGTGTTCAGTATGCTATAAAGAACATCTTCAAAGA
NM_001242912.1      TATGGAAACCCTCGTACAAATGGCATGTGTTCAGTATGCTATAAAGAACATCTTCAAAGA
NM_001242917.1      TATGGAAACCCTCGTACAAATGGCATGTGTTCAGTATGCTATAAAGAACATCTTCAAAGA
                    ************************************************************

HsCD00329751        CAGAATAGTAGTAATGGTAGAATAAGCCCACCTGCAACCTCTGTCAGTAGTCTGTCTGAA
NM_001242913.1      CAGAATAGTAGTAATGGTAGAATAAGCCCACCTGCAACCTCTGTCAGTAGTCTGTCTGAA
NM_001242916.1      CAGAATAGTAGTAATGGTAGAATAAGCCCACCTGCAACCTCTGTCAGTAGTCTGTCTGAA
NM_019006.3         CAGAATAGTAGTAATGGTAGAATAAGCCCACCTGCAACCTCTGTCAGTAGTCTGTCTGAA
NM_001242915.1      CAGAATAGTAGTAATGGTAGAATAAGCCCACCTGCAACCTCTGTCAGTAGTCTGTCTGAA
NM_001242914.1      CAGAATAGTAGTAATGGTAGAATAAGCCCACCTGCAACCTCTGTCAGTAGTCTGTCTGAA
NM_001242911.1      CAGAATAGTAGTAATGGTAGAATAAGCCCACCTGCAACCTCTGTCAGTAGTCTGTCTGAA
NM_001242912.1      CAGAATAGTAGTAATGGTAGAATAAGCCCACCTGCAACCTCTGTCAGTAGTCTGTCTGAA
NM_001242917.1      CAGAATAGTAGTAATGGTAGAATAAGCCCACCT---------------------------
                    *********************************                           

HsCD00329751        TCTTTACCAGTTCAATGCACAGATGGCAGTGTGCCAGAAGCCCAGTCAGCATTAGACTCT
NM_001242913.1      TCTTTACCAGTTCAATGCACAGATGGCAGTGTGCCAGAAGCCCAGTCAGCATTAGACTCT
NM_001242916.1      TCTTTACCAGTTCAATGCACAGATGGCAGTGTGCCAGAAGCCCAGTCAGCATTAGACTCT
NM_019006.3         TCTTTACCAGTTCAATGCACAGATGGCAGTGTGCCAGAAGCCCAGTCAGCATTAGACTCT
NM_001242915.1      TCTTTACCAGTTCAATGCACAGATGGCAGTGTGCCAGAAGCCCAGTCAGCATTAGACTCT
NM_001242914.1      TCTTTACCAGTTCAATGCACAGATGGCAGTGTGCCAGAAGCCCAGTCAGCATTAGACTCT
NM_001242911.1      TCTTTACCAGTTCAATGCACAGATGGCAGTGTGCCAGAAGCCCAGTCAGCATTAGACTCT
NM_001242912.1      TCTTTACCAGTTCAATGCACAGATGGCAGTGTGCCAGAAGCCCAGTCAGCATTAGACTCT
NM_001242917.1      ---------GTTCAATGCACAGATGGCAGTGTGCCAGAAGCCCAGTCAGCATTAGACTCT
                             ***************************************************

HsCD00329751        ACATCTTCATCTATGCAGCCCAGCCCTGTATCAAATCAGTCACTTTTATCAGAATCTGTA
NM_001242913.1      ACATCTTCATCTATGCAGCCCAGCCCTGTATCAAATCAGTCACTTTTATCAGAATCTGTA
NM_001242916.1      ACATCTTCATCTATGCAGCCCAGCCCTGTATCAAATCAGTCACTTTTATCAGAATCTGTA
NM_019006.3         ACATCTTCATCTATGCAGCCCAGCCCTGTATCAAATCAGTCACTTTTATCAGAATCTGTA
NM_001242915.1      ACATCTTCATCTATGCAGCCCAGCCCTGTATCAAATCAGTCACTTTTATCAGAATCTGTA
NM_001242914.1      ACATCTTCATCTATGCAGCCCAGCCCTGTATCAAATCAGTCACTTTTATCAGAATCTGTA
NM_001242911.1      ACATCTTCATCTATGCAGCCCAGCCCTGTATCAAATCAGTCACTTTTATCAGAATCTGTA
NM_001242912.1      ACATCTTCATCTATGCAGCCCAGCCCTGTATCAAATCAGTCACTTTTATCAGAATCTGTA
NM_001242917.1      ACATCTTCATCTATGCAGCCCAGCCCTGTATCAAATCAGTCACTTTTATCAGAATCTGTA
                    ************************************************************

HsCD00329751        GCATCTTCTCAATTGGACAGTACATCTGTGGACAAAGCAGTACCTGAAACAGAAGATGTG
NM_001242913.1      GCATCTTCTCAATTGGACAGTACATCTGTGGACAAAGCAGTACCTGAAACAGAAGATGTG
NM_001242916.1      GCATCTTCTCAATTGGACAGTACATCTGTGGACAAAGCAGTACCTGAAACAGAAGATGTG
NM_019006.3         GCATCTTCTCAATTGGACAGTACATCTGTGGACAAAGCAGTACCTGAAACAGAAGATGTG
NM_001242915.1      GCATCTTCTCAATTGGACAGTACATCTGTGGACAAAGCAGTACCTGAAACAGAAGATGTG
NM_001242914.1      GCATCTTCTCAATTGGACAGTACATCTGTGGACAAAGCAGTACCTGAAACAGAAGATGTG
NM_001242911.1      GCATCTTCTCAATTGGACAGTACATCTGTGGACAAAGCAGTACCTGAAACAGAAGATGTG
NM_001242912.1      GCATCTTCTCAATTGGACAGTACATCTGTGGACAAAGCAGTACCTGAAACAGAAGATGTG
NM_001242917.1      GCATCTTCTCAATTGGACAGTACATCTGTGGACAAAGCAGTACCTGAAACAGAAGATGTG
                    ************************************************************

HsCD00329751        CAGGCTTCAGTATCAGACACAGCACAGCAGCCATCTGAAGAGCAAAGCAAGTCTCTTGAA
NM_001242913.1      CAGGCTTCAGTATCAGACACAGCACAGCAGCCATCTGAAGAGCAAAGCAAGTCTCTTGAA
NM_001242916.1      CAGGCTTCAGTATCAGACACAGCACAGCAGCCATCTGAAGAGCAAAGCAAGTCTCTTGAA
NM_019006.3         CAGGCTTCAGTATCAGACACAGCACAGCAGCCATCTGAAGAGCAAAGCAAGTCTCTTGAA
NM_001242915.1      CAGGCTTCAGTATCAGACACAGCACAGCAGCCATCTGAAGAGCAAAGCAAGTCTCTTGAA
NM_001242914.1      CAGGCTTCAGTATCAGACACAGCACAGCAGCCATCTGAAGAGCAAAGCAAGTCTCTTGAA
NM_001242911.1      CAGGCTTCAGTATCAGACACAGCACAGCAGCCATCTGAAGAGCAAAGCAAGTCTCTTGAA
NM_001242912.1      CAGGCTTCAGTATCAGACACAGCACAGCAGCCATCTGAAGAGCAAAGCAAGTCTCTTGAA
NM_001242917.1      CAGGCTTCAGTATCAGACACAGCACAGCAGCCATCTGAAGAGCAAAGCAAGTCTCTTGAA
                    ************************************************************

HsCD00329751        AAACCGAAACAAAAAAAGAATCGCTGTTTCATGTGCAGGAAGAAAGTGGGACTTACTGGG
NM_001242913.1      AAACCGAAACAAAAAAAGAATCGCTGTTTCATGTGCAGGAAGAAAGTGGGACTTACTGGG
NM_001242916.1      AAACCGAAACAAAAAAAGAATCGCTGTTTCATGTGCAGGAAGAAAGTGGGACTTACTGGG
NM_019006.3         AAACCGAAACAAAAAAAGAATCGCTGTTTCATGTGCAGGAAGAAAGTGGGACTTACTGGG
NM_001242915.1      AAACCGAAACAAAAAAAGAATCGCTGTTTCATGTGCAGGAAGAAAGTGGGACTTACTGGG
NM_001242914.1      AAACCGAAACAAAAAAAGAATCGCTGTTTCATGTGCAGGAAGAAAGTGGGACTTACTGGG
NM_001242911.1      AAACCGAAACAAAAAAAGAATCGCTGTTTCATGTGCAGGAAGAAAGTGGGACTTACTGGG
NM_001242912.1      AAACCGAAACAAAAAAAGAATCGCTGTTTCATGTGCAGGAAGAAAGTGGGACTTACTGGG
NM_001242917.1      AAACCGAAACAAAAAAAGAATCGCTGTTTCATGTGCAGGAAGAAAGTGGGACTTACTGGG
                    ************************************************************

HsCD00329751        TTTGAATGCCGGTGTGGAAATGTTTACTGTGGTGTACACCGTTACTCAGATGTACACAAT
NM_001242913.1      TTTGAATGCCGGTGTGGAAATGTTTACTGTGGTGTACACCGTTACTCAGATGTACACAAT
NM_001242916.1      TTTGAATGCCGGTGTGGAAATGTTTACTGTGGTGTACACCGTTACTCAGATGTACACAAT
NM_019006.3         TTTGAATGCCGGTGTGGAAATGTTTACTGTGGTGTACACCGTTACTCAGATGTACACAAT
NM_001242915.1      TTTGAATGCCGGTGTGGAAATGTTTACTGTGGTGTACACCGTTACTCAGATGTACACAAT
NM_001242914.1      TTTGAATGCCGGTGTGGAAATGTTTACTGTGGTGTACACCGTTACTCAGATGTACACAAT
NM_001242911.1      TTTGAATGCCGGTGTGGAAATGTTTACTGTGGTGTACACCGTTACTCAGATGTACACAAT
NM_001242912.1      TTTGAATGCCGGTGTGGAAATGTTTACTGTGGTGTACACCGTTACTCAGATGTACACAAT
NM_001242917.1      TTTGAATGCCGGTGTGGAAATGTTTACTGTGGTGTACACCGTTACTCAGATGTACACAAT
                    ************************************************************

HsCD00329751        TGCTCTTACAATTACAAAGCCGATGCTGCTGAGAAAATCAGAAAAGAAAATCCAGTAGTT
NM_001242913.1      TGCTCTTACAATTACAAAGCCGATGCTGCTGAGAAAATCAGAAAAGAAAATCCAGTAGTT
NM_001242916.1      TGCTCTTACAATTACAAAGCCGATGCTGCTGAGAAAATCAGAAAAGAAAATCCAGTAGTT
NM_019006.3         TGCTCTTACAATTACAAAGCCGATGCTGCTGAGAAAATCAGAAAAGAAAATCCAGTAGTT
NM_001242915.1      TGCTCTTACAATTACAAAGCCGATGCTGCTGAGAAAATCAGAAAAGAAAATCCAGTAGTT
NM_001242914.1      TGCTCTTACAATTACAAAGCCGATGCTGCTGAGAAAATCAGAAAAGAAAATCCAGTAGTT
NM_001242911.1      TGCTCTTACAATTACAAAGCCGATGCTGCTGAGAAAATCAGAAAAGAAAATCCAGTAGTT
NM_001242912.1      TGCTCTTACAATTACAAAGCCGATGCTGCTGAGAAAATCAGAAAAGAAAATCCAGTAGTT
NM_001242917.1      TGCTCTTACAATTACAAAGCCGATGCTGCTGAGAAAATCAGAAAAGAAAATCCAGTAGTT
                    ************************************************************

HsCD00329751        GTTGGTGAAAAGATCCAAAAGATTTTG
NM_001242913.1      GTTGGTGAAAAGATCCAAAAGATTTGA
NM_001242916.1      GTTGGTGAAAAGATCCAAAAGATTTGA
NM_019006.3         GTTGGTGAAAAGATCCAAAAGATTTGA
NM_001242915.1      GTTGGTGAAAAGATCCAAAAGATTTGA
NM_001242914.1      GTTGGTGAAAAGATCCAAAAGATTTGA
NM_001242911.1      GTTGGTGAAAAGATCCAAAAGATTTGA
NM_001242912.1      GTTGGTGAAAAGATCCAAAAGATTTGA
NM_001242917.1      GTTGGTGAAAAGATCCAAAAGATTTGA
                    ************************* .

Protein

SMART domain : ZnF_A20 : A20-like zinc fingers
SMART domain : ZnF_AN1 : AN1-like Zinc finger
UniProt : Q6FIF0
HPRD : 18314

Clone

Cloning Information : 268492
HIP Master Clone ID : 193541
Original Clone ID : FLH268492.01L

Organism


TAX_ID : 9606
Species Specific ID: 54469

Genome

Chromosome : 15
Map Location : 15q25.1
Ensembl : ENSG00000086666

Reagents

Labome : AWP1-antibody