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C14orf166 (H. sapiens ) in pLenti6.2/V5-DEST

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Explanation of Terms


Gene: Gene Symbol:  C14orf166
Gene Name:  chromosome 14 open reading frame 166
Sequence             
Original Clone ID: FLH268497.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329756

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 735nts         Open reading frame : 1 to 735
Reference Sequence Alignment

ATGTTCCGACGCAAGTTGACGGCTCTCGACTACCACAACCCCGCCGGCTTCAACTGCAAA
GATGAAACAGAATTTAGAAACTTCATCGTTTGGCTTGAAGACCAGAAAATCAGGCACTAC
AAGATTGAAGACAGAGGGAATTTAAGAAACATCCACAGCAGCGACTGGCCCAAGTTCTTT
GAAAAGTATCTCAGAGATGTTAACTGTCCTTTCAAGATTCAAGATCGACAAGAAGCTATT
GACTGGCTTCTTGGTTTAGCTGTTAGACTTGAATATGGAGATAATGCTGAAAAATACAAG
GATTTAGTACCTGATAATTCAAAAACTGCTGACAATGCAACTAAAAATGCAGAACCATTG
ATCAATTTGGATGTAAATAATCCTGATTTTAAGGCTGGTGTGATGGCTTTGGCTAACCTG
CTTCAGATTCAGCGTCATGATGATTACCTGGTAATGCTTAAGGCAATTCGGATTTTGGTT
CAGGAGCGCCTGACACAGGATGCAGTTGCTAAGGCAAATCAAACAAAAGAGGGCTTACCT
GTTGCTTTAGACAAACATATTCTTGGTTTTGACACAGGAGATGCAGTTCTTAATGAAGCT
GCTCAAATTCTGCGATTGCTGCACATAGAGGAGCTCAGAGAGCTACAGACAAAAATCAAC
GAAGCCATAGTAGCTGTTCAGGCAATTATTGCTGATCCAAAGACAGACCACAGACTGGGA
AAAGTTGGAAGATTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 817
Start on reference sequence 83
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : C14orf166
Symbol Nomenclature : C14orf166
SYNONYM : CGI99; CLE; CLE7; LCRP369; RLLM1
Designation : CLE7 homolog|RLL motif containing 1|UPF0568 protein C14orf166
Full Nomenclature : chromosome 14 open reading frame 166
GENEID : 51637
Locus Tag : CGI-99
HGNC : 23169
MIM : 610858
Vega : OTTHUMG00000152332
Target GenBank: BC001722

 

Reference Sequence Alignment

  

HsCD00329756      ATGTTCCGACGCAAGTTGACGGCTCTCGACTACCACAACCCCGCCGGCTTCAACTGCAAA
NM_016039.2       ATGTTCCGACGCAAGTTGACGGCTCTCGACTACCACAACCCCGCCGGCTTCAACTGCAAA
                  ************************************************************

HsCD00329756      GATGAAACAGAATTTAGAAACTTCATCGTTTGGCTTGAAGACCAGAAAATCAGGCACTAC
NM_016039.2       GATGAAACAGAATTTAGAAACTTCATCGTTTGGCTTGAAGACCAGAAAATCAGGCACTAC
                  ************************************************************

HsCD00329756      AAGATTGAAGACAGAGGGAATTTAAGAAACATCCACAGCAGCGACTGGCCCAAGTTCTTT
NM_016039.2       AAGATTGAAGACAGAGGGAATTTAAGAAACATCCACAGCAGCGACTGGCCCAAGTTCTTT
                  ************************************************************

HsCD00329756      GAAAAGTATCTCAGAGATGTTAACTGTCCTTTCAAGATTCAAGATCGACAAGAAGCTATT
NM_016039.2       GAAAAGTATCTCAGAGATGTTAACTGTCCTTTCAAGATTCAAGATCGACAAGAAGCTATT
                  ************************************************************

HsCD00329756      GACTGGCTTCTTGGTTTAGCTGTTAGACTTGAATATGGAGATAATGCTGAAAAATACAAG
NM_016039.2       GACTGGCTTCTTGGTTTAGCTGTTAGACTTGAATATGGAGATAATGCTGAAAAATACAAG
                  ************************************************************

HsCD00329756      GATTTAGTACCTGATAATTCAAAAACTGCTGACAATGCAACTAAAAATGCAGAACCATTG
NM_016039.2       GATTTAGTACCTGATAATTCAAAAACTGCTGACAATGCAACTAAAAATGCAGAACCATTG
                  ************************************************************

HsCD00329756      ATCAATTTGGATGTAAATAATCCTGATTTTAAGGCTGGTGTGATGGCTTTGGCTAACCTG
NM_016039.2       ATCAATTTGGATGTAAATAATCCTGATTTTAAGGCTGGTGTGATGGCTTTGGCTAACCTG
                  ************************************************************

HsCD00329756      CTTCAGATTCAGCGTCATGATGATTACCTGGTAATGCTTAAGGCAATTCGGATTTTGGTT
NM_016039.2       CTTCAGATTCAGCGTCATGATGATTACCTGGTAATGCTTAAGGCAATTCGGATTTTGGTT
                  ************************************************************

HsCD00329756      CAGGAGCGCCTGACACAGGATGCAGTTGCTAAGGCAAATCAAACAAAAGAGGGCTTACCT
NM_016039.2       CAGGAGCGCCTGACACAGGATGCAGTTGCTAAGGCAAATCAAACAAAAGAGGGCTTACCT
                  ************************************************************

HsCD00329756      GTTGCTTTAGACAAACATATTCTTGGTTTTGACACAGGAGATGCAGTTCTTAATGAAGCT
NM_016039.2       GTTGCTTTAGACAAACATATTCTTGGTTTTGACACAGGAGATGCAGTTCTTAATGAAGCT
                  ************************************************************

HsCD00329756      GCTCAAATTCTGCGATTGCTGCACATAGAGGAGCTCAGAGAGCTACAGACAAAAATCAAC
NM_016039.2       GCTCAAATTCTGCGATTGCTGCACATAGAGGAGCTCAGAGAGCTACAGACAAAAATCAAC
                  ************************************************************

HsCD00329756      GAAGCCATAGTAGCTGTTCAGGCAATTATTGCTGATCCAAAGACAGACCACAGACTGGGA
NM_016039.2       GAAGCCATAGTAGCTGTTCAGGCAATTATTGCTGATCCAAAGACAGACCACAGACTGGGA
                  ************************************************************

HsCD00329756      AAAGTTGGAAGATTG
NM_016039.2       AAAGTTGGAAGATGA
                  ************* .

Protein

UniProt : Q549M8
UniProt : Q9Y224
HPRD : 16615

Clone

Cloning Information : 268497
HIP Master Clone ID : 193551
Original Clone ID : FLH268497.01L

Organism


TAX_ID : 9606
Species Specific ID: 51637

Genome

Chromosome : 14
Map Location : 14q22.1
Ensembl : ENSG00000087302

Reagents

Labome : CGI-99-antibody