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PPP2R5B (H. sapiens ) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  PPP2R5B
Gene Name:  protein phosphatase 2, regulatory subunit B', beta
Sequence             
Original Clone ID: FLH268498.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329757

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1494nts         Open reading frame : 1 to 1494
Reference Sequence Alignment

ATGGAGACGAAGCTGCCCCCTGCAAGCACCCCCACTAGCCCCTCCTCCCCCGGGCTGTCG
CCTGTGCCCCCACCCGACAAGGTGGACGGCTTCTCCCGCCGTTCCCTCCGCAGAGCCCGG
CCCCGCCGCTCCCACAGCTCCTCTCAGTTCCGCTATCAGAGCAACCAGCAAGAGCTCACA
CCGCTGCCCCTGCTCAAAGATGTGCCGGCTTCCGAGCTGCACGAGCTGCTGAGCCGGAAG
CTGGCCCAGTGTGGGGTGATGTTTGACTTCTTGGACTGTGTGGCCGACCTCAAGGGGAAG
GAGGTGAAGCGGGCAGCCCTCAACGAGCTGGTGGAGTGTGTGGGGAGCACCCGGGGTGTC
CTCATCGAGCCCGTCTACCCAGACATCATCCGCATGATCTCAGTGAATATCTTCCGGACT
CTGCCGCCCAGTGAGAACCCTGAATTTGACCCTGAAGAGGATGAGCCCAATCTTGAGCCT
TCGTGGCCACACCTGCAGCTGGTATATGAGTTTTTCCTGCGTTTCTTGGAGAGCCCAGAC
TTCCAGCCCTCCGTGGCCAAGAGATATGTGGATCAAAAGTTTGTCCTGATGCTCCTGGAG
CTATTTGATAGTGAGGATCCCCGGGAGCGTGAGTACCTCAAGACCATCCTGCACCGGGTC
TATGGCAAGTTCCTGGGTCTCCGGGCCTACATCCGCAAACAGTGCAACCACATCTTCCTC
CGGTTCATCTATGAATTCGAGCACTTCAATGGTGTGGCTGAGCTGCTGGAGATCCTAGGA
AGCATCATCAATGGCTTTGCGCTGCCCCTGAAGACGGAGCACAAGCAGTTCCTGGTTCGC
GTCCTGATCCCCCTGCACTCTGTCAAGTCGCTGTCTGTCTTCCATGCCCAGCTGGCATAC
TGTGTGGTGCAGTTCCTGGAGAAGGATGCCACTCTGACAGAGCACGTGATCCGGGGGCTG
CTCAAATACTGGCCAAAAACCTGCACCCAGAAGGAGGTGATGTTTCTGGGGGAGATGGAA
GAGATTCTTGATGTCATCGAGCCCTCCCAGTTTGTGAAGATCCAGGAGCCCCTTTTTAAG
CAGGTGGCTCGCTGTGTTTCCAGCCCCCATTTCCAGGTTGCAGAGCGGGCTCTGTATTTC
TGGAACAATGAGTATATCCTAAGCCTCATTGAGGACAACTGCCACACTGTGCTGCCTGCT
GTGTTTGGGACCCTCTACCAAGTCTCCAAGGAGCACTGGAACCAAACCATCGTATCACTG
ATCTACAATGTGCTCAAGACCTTCATGGAGATGAATGGGAAGCTGTTTGATGAGCTCACA
GCCTCCTACAAGCTGGAAAAGCAGCAGGAGCAGCAGAAGGCCCAGGAGCGTCAGGAGTTA
TGGCAAGGTCTGGAGGAGCTGCGGCTACGCCGGCTACAGGGGACCCAGGGGGCCAAGGAG
GCCCCCCTCCAGCGGCTTACACCCCAGGTGGCCGCCAGTGGGGGTCAGAGCTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 2110
Start on reference sequence 617
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : PPP2R5B
Symbol Nomenclature : PPP2R5B
SYNONYM : B56B; PR61B
Designation : PP2A B subunit isoform B'-beta|PP2A B subunit isoform B56-beta|PP2A B subunit isoform PR61-beta|PP2A B subunit isoform R5-beta|protein phosphatase 2, regulatory subunit B', beta isoform|serine/threonine protein phosphatase 2A, 56 kDa regulatory subunit, beta isoform|serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit beta isoform
Full Nomenclature : protein phosphatase 2, regulatory subunit B', beta
GENEID : 5526
HGNC : 9310
MIM : 601644
Vega : OTTHUMG00000150043
Target GenBank: BC045619

 

Reference Sequence Alignment

  

HsCD00329757      ATGGAGACGAAGCTGCCCCCTGCAAGCACCCCCACTAGCCCCTCCTCCCCCGGGCTGTCG
NM_006244.3       ATGGAGACGAAGCTGCCCCCTGCAAGCACCCCCACTAGCCCCTCCTCCCCCGGGCTGTCG
                  ************************************************************

HsCD00329757      CCTGTGCCCCCACCCGACAAGGTGGACGGCTTCTCCCGCCGTTCCCTCCGCAGAGCCCGG
NM_006244.3       CCTGTGCCCCCACCCGACAAGGTGGACGGCTTCTCCCGCCGTTCCCTCCGCAGAGCCCGG
                  ************************************************************

HsCD00329757      CCCCGCCGCTCCCACAGCTCCTCTCAGTTCCGCTATCAGAGCAACCAGCAAGAGCTCACA
NM_006244.3       CCCCGCCGCTCCCACAGCTCCTCTCAGTTCCGCTATCAGAGCAACCAGCAAGAGCTCACA
                  ************************************************************

HsCD00329757      CCGCTGCCCCTGCTCAAAGATGTGCCGGCTTCCGAGCTGCACGAGCTGCTGAGCCGGAAG
NM_006244.3       CCGCTGCCCCTGCTCAAAGATGTGCCGGCTTCCGAGCTGCACGAGCTGCTGAGCCGGAAG
                  ************************************************************

HsCD00329757      CTGGCCCAGTGTGGGGTGATGTTTGACTTCTTGGACTGTGTGGCCGACCTCAAGGGGAAG
NM_006244.3       CTGGCCCAGTGTGGGGTGATGTTTGACTTCTTGGACTGTGTGGCCGACCTCAAGGGGAAG
                  ************************************************************

HsCD00329757      GAGGTGAAGCGGGCAGCCCTCAACGAGCTGGTGGAGTGTGTGGGGAGCACCCGGGGTGTC
NM_006244.3       GAGGTGAAGCGGGCAGCCCTCAACGAGCTGGTGGAGTGTGTGGGGAGCACCCGGGGTGTC
                  ************************************************************

HsCD00329757      CTCATCGAGCCCGTCTACCCAGACATCATCCGCATGATCTCAGTGAATATCTTCCGGACT
NM_006244.3       CTCATCGAGCCCGTCTACCCAGACATCATCCGCATGATCTCAGTGAATATCTTCCGGACT
                  ************************************************************

HsCD00329757      CTGCCGCCCAGTGAGAACCCTGAATTTGACCCTGAAGAGGATGAGCCCAATCTTGAGCCT
NM_006244.3       CTGCCGCCCAGTGAGAACCCTGAATTTGACCCTGAAGAGGATGAGCCCAATCTTGAGCCT
                  ************************************************************

HsCD00329757      TCGTGGCCACACCTGCAGCTGGTATATGAGTTTTTCCTGCGTTTCTTGGAGAGCCCAGAC
NM_006244.3       TCGTGGCCACACCTGCAGCTGGTATATGAGTTTTTCCTGCGTTTCTTGGAGAGCCCAGAC
                  ************************************************************

HsCD00329757      TTCCAGCCCTCCGTGGCCAAGAGATATGTGGATCAAAAGTTTGTCCTGATGCTCCTGGAG
NM_006244.3       TTCCAGCCCTCCGTGGCCAAGAGATATGTGGATCAAAAGTTTGTCCTGATGCTCCTGGAG
                  ************************************************************

HsCD00329757      CTATTTGATAGTGAGGATCCCCGGGAGCGTGAGTACCTCAAGACCATCCTGCACCGGGTC
NM_006244.3       CTATTTGATAGTGAGGATCCCCGGGAGCGTGAGTACCTCAAGACCATCCTGCACCGGGTC
                  ************************************************************

HsCD00329757      TATGGCAAGTTCCTGGGTCTCCGGGCCTACATCCGCAAACAGTGCAACCACATCTTCCTC
NM_006244.3       TATGGCAAGTTCCTGGGTCTCCGGGCCTACATCCGCAAACAGTGCAACCACATCTTCCTC
                  ************************************************************

HsCD00329757      CGGTTCATCTATGAATTCGAGCACTTCAATGGTGTGGCTGAGCTGCTGGAGATCCTAGGA
NM_006244.3       CGGTTCATCTATGAATTCGAGCACTTCAATGGTGTGGCTGAGCTGCTGGAGATCCTAGGA
                  ************************************************************

HsCD00329757      AGCATCATCAATGGCTTTGCGCTGCCCCTGAAGACGGAGCACAAGCAGTTCCTGGTTCGC
NM_006244.3       AGCATCATCAATGGCTTTGCGCTGCCCCTGAAGACGGAGCACAAGCAGTTCCTGGTTCGC
                  ************************************************************

HsCD00329757      GTCCTGATCCCCCTGCACTCTGTCAAGTCGCTGTCTGTCTTCCATGCCCAGCTGGCATAC
NM_006244.3       GTCCTGATCCCCCTGCACTCTGTCAAGTCGCTGTCTGTCTTCCATGCCCAGCTGGCATAC
                  ************************************************************

HsCD00329757      TGTGTGGTGCAGTTCCTGGAGAAGGATGCCACTCTGACAGAGCACGTGATCCGGGGGCTG
NM_006244.3       TGTGTGGTGCAGTTCCTGGAGAAGGATGCCACTCTGACAGAGCACGTGATCCGGGGGCTG
                  ************************************************************

HsCD00329757      CTCAAATACTGGCCAAAAACCTGCACCCAGAAGGAGGTGATGTTTCTGGGGGAGATGGAA
NM_006244.3       CTCAAATACTGGCCAAAAACCTGCACCCAGAAGGAGGTGATGTTTCTGGGGGAGATGGAA
                  ************************************************************

HsCD00329757      GAGATTCTTGATGTCATCGAGCCCTCCCAGTTTGTGAAGATCCAGGAGCCCCTTTTTAAG
NM_006244.3       GAGATTCTTGATGTCATCGAGCCCTCCCAGTTTGTGAAGATCCAGGAGCCCCTTTTTAAG
                  ************************************************************

HsCD00329757      CAGGTGGCTCGCTGTGTTTCCAGCCCCCATTTCCAGGTTGCAGAGCGGGCTCTGTATTTC
NM_006244.3       CAGGTGGCTCGCTGTGTTTCCAGCCCCCATTTCCAGGTTGCAGAGCGGGCTCTGTATTTC
                  ************************************************************

HsCD00329757      TGGAACAATGAGTATATCCTAAGCCTCATTGAGGACAACTGCCACACTGTGCTGCCTGCT
NM_006244.3       TGGAACAATGAGTATATCCTAAGCCTCATTGAGGACAACTGCCACACTGTGCTGCCTGCT
                  ************************************************************

HsCD00329757      GTGTTTGGGACCCTCTACCAAGTCTCCAAGGAGCACTGGAACCAAACCATCGTATCACTG
NM_006244.3       GTGTTTGGGACCCTCTACCAAGTCTCCAAGGAGCACTGGAACCAAACCATCGTATCACTG
                  ************************************************************

HsCD00329757      ATCTACAATGTGCTCAAGACCTTCATGGAGATGAATGGGAAGCTGTTTGATGAGCTCACA
NM_006244.3       ATCTACAATGTGCTCAAGACCTTCATGGAGATGAATGGGAAGCTGTTTGATGAGCTCACA
                  ************************************************************

HsCD00329757      GCCTCCTACAAGCTGGAAAAGCAGCAGGAGCAGCAGAAGGCCCAGGAGCGTCAGGAGTTA
NM_006244.3       GCCTCCTACAAGCTGGAAAAGCAGCAGGAGCAGCAGAAGGCCCAGGAGCGTCAGGAGTTA
                  ************************************************************

HsCD00329757      TGGCAAGGTCTGGAGGAGCTGCGGCTACGCCGGCTACAGGGGACCCAGGGGGCCAAGGAG
NM_006244.3       TGGCAAGGTCTGGAGGAGCTGCGGCTACGCCGGCTACAGGGGACCCAGGGGGCCAAGGAG
                  ************************************************************

HsCD00329757      GCCCCCCTCCAGCGGCTTACACCCCAGGTGGCCGCCAGTGGGGGTCAGAGCTTG
NM_006244.3       GCCCCCCTCCAGCGGCTTACACCCCAGGTGGCCGCCAGTGGGGGTCAGAGCTAG
                  ****************************************************:*

Pathways

Panther : EGF receptor signaling pathway
Panther : FGF signaling pathway
Panther : Wnt signaling pathway
Reactome : Adaptive Immune System
Reactome : Beta-catenin phosphorylation cascade
Reactome : CTLA4 inhibitory signaling
Reactome : Cell Cycle
Reactome : Cell Cycle, Mitotic
Reactome : Costimulation by the CD28 family
Reactome : Degradation of beta-catenin by the destruction complex
Reactome : Hemostasis
Reactome : IRE1alpha activates chaperones
Reactome : Immune System
Reactome : M Phase
Reactome : Metabolism of proteins
Reactome : Mitotic Anaphase
Reactome : Mitotic M-M/G1 phases
Reactome : Mitotic Metaphase and Anaphase
Reactome : Mitotic Prometaphase
Reactome : Platelet homeostasis
Reactome : Platelet sensitization by LDL
Reactome : Resolution of Sister Chromatid Cohesion
Reactome : Separation of Sister Chromatids
Reactome : Signal Transduction
Reactome : Signaling by Wnt
Reactome : TCF dependent signaling in response to WNT
Reactome : Unfolded Protein Response (UPR)
Reactome : XBP1(S) activates chaperone genes
Reactome : disassembly of the destruction complex and recruitment of AXIN to the membrane
WikiPathway : Glycogen Metabolism

Protein

UniProt : Q15173
HPRD : 09038

Clone

Cloning Information : 268498
HIP Master Clone ID : 193552
Original Clone ID : FLH268498.01L

Organism


TAX_ID : 9606
Species Specific ID: 5526

Genome

Chromosome : 11
Map Location : 11q12
Ensembl : ENSG00000068971

Reagents

Labome : PPP2R5B-antibody