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CTDSP1 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  CTDSP1
Gene Name:  CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase 1
Sequence             
Original Clone ID: FLH268501.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329760

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 786nts         Open reading frame : 1 to 786
Reference Sequence Alignment

ATGGACAGCTCGGCCGTCATTACTCAGATCAGCAAGGAGGAGGCTCGGGGCCCGCTGCGG
GGCAAAGGTGACCAGAAGTCAGCAGCTTCCCAGAAGCCCCGAAGCCGGGGCATCCTCCAC
TCACTCTTCTGCTGTGTCTGCCGGGATGATGGGGAGGCCCTGCCTGCTCACAGCGGGGCG
CCCCTGCTTGTGGAGGAGAATGGAGCCATCCCTAAGCAGACCCCAGTCCAATACCTGCTC
CCTGAGGCCAAGGCCCAGGACTCAGACAAGATCTGCGTGGTCATCGACCTGGACGAGACC
CTGGTGCACAGCTCCTTCAAGCCAGTGAACAACGCGGACTTCATCATCCCTGTGGAGATT
GATGGGGTGGTCCACCAGGTCTACGTGTTGAAGCGTCCTCACGTGGATGAGTTCCTGCAG
CGAATGGGCGAGCTCTTTGAATGTGTGCTGTTCACTGCTAGCCTCGCCAAGTACGCAGAC
CCAGTAGCTGACCTGCTGGACAAATGGGGGGCCTTCCGGGCCCGGCTGTTTCGAGAGTCC
TGCGTCTTCCACCGGGGGAACTACGTGAAGGACCTGAGCCGGTTGGGTCGAGACCTGCGG
CGGGTGCTCATCCTGGACAATTCACCTGCCTCCTATGTCTTCCATCCAGACAATGCTGTA
CCGGTGGCCTCGTGGTTTGACAACATGAGTGACACAGAGCTCCACGACCTCCTCCCCTTC
TTCGAGCAACTCAGCCGTGTGGACGACGTGTACTCAGTGCTCAGGCAGCCACGGCCAGGG
AGCTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 840
Start on reference sequence 55
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CTDSP1
Symbol Nomenclature : CTDSP1
SYNONYM : NIF3; NLI-IF; NLIIF; SCP1
Designation : NLI-interacting factor 3|carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1|nuclear LIM interactor-interacting factor 3|small C-terminal domain phosphatase 1
Full Nomenclature : CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase 1
GENEID : 58190
HGNC : 21614
MIM : 605323
Vega : OTTHUMG00000133109
Target GenBank: BC012977

 

Reference Sequence Alignment

  

HsCD00329760        ATGGACAGCTCGGCCGTCATTACTCAGATCAGCAAGGAGGAGGCTCGGGGCCCGCTGCGG
NM_021198.2         ATGGACAGCTCGGCCGTCATTACTCAGATCAGCAAGGAGGAGGCTCGGGGCCCGCTGCGG
NM_182642.2         ATGGACAGCTCGGCCGTCATTACTCAGATCAGCAAGGAGGAGGCTCGGGGCCCGCTGCGG
NM_001206878.1      ATGGTGGCCGCCCCGTGGGCTACCCAGGAGCAGGAGGAGGGCCGAGGGATCCAGCCCGGG
                    ****: . * *  *    . *** ***.: .. .******.   : **. **.**   **

HsCD00329760        GGCAAAGGTGACCAGAAGTCAGCAGCTTCCCAGAAGCCCCGAAGCCGGGGCATCCTCCAC
NM_021198.2         GGCAAAGGTGACCAGAAGTCAGCAGCTTCCCAGAAGCCCCGAAGCCGGGGCATCCTCCAC
NM_182642.2         GGCAAAGGTGACCAGAAGTCAGCAGCTTCCCAGAAGCCCCGAAGCCGGGGCATCCTCCAC
NM_001206878.1      GACCGGGGTGACCAGAAGTCAGCAGCTTCCCAGAAGCCCCGAAGCCGGGGCATCCTCCAC
                    *.*...******************************************************

HsCD00329760        TCACTCTTCTGCTGTGTCTGCCGGGATGATGGGGAGGCCCTGCCTGCTCACAGCGGGGCG
NM_021198.2         TCACTCTTCTGCTGTGTCTGCCGGGATGATGGGGAGGCCCTGCCTGCTCACAGCGGGGCG
NM_182642.2         TCACTCTTCTGCTGTGTCTGCCGGGATGATGGGGAGGCCCTGCCTGCTCACAGCGGGGCG
NM_001206878.1      TCACTCTTCTGCTGTGTCTGCCGGGATGATGGGGAGGCCCTGCCTGCTCACAGCGGGGCG
                    ************************************************************

HsCD00329760        CCCCTGCTTGTGGAGGAGAATGGAGCCATCCCTAAGCAGACCCCAGTCCAATACCTGCTC
NM_021198.2         CCCCTGCTTGTGGAGGAGAATGGCGCCATCCCTAAGCAGACCCCAGTCCAATACCTGCTC
NM_182642.2         CCCCTGCTTGTGGAGGAGAATGGCGCCATCCCTAAG---ACCCCAGTCCAATACCTGCTC
NM_001206878.1      CCCCTGCTTGTGGAGGAGAATGGCGCCATCCCTAAG---ACCCCAGTCCAATACCTGCTC
                    ***********************.************   *********************

HsCD00329760        CCTGAGGCCAAGGCCCAGGACTCAGACAAGATCTGCGTGGTCATCGACCTGGACGAGACC
NM_021198.2         CCTGAGGCCAAGGCCCAGGACTCAGACAAGATCTGCGTGGTCATCGACCTGGACGAGACC
NM_182642.2         CCTGAGGCCAAGGCCCAGGACTCAGACAAGATCTGCGTGGTCATCGACCTGGACGAGACC
NM_001206878.1      CCTGAGGCCAAGGCCCAGGACTCAGACAAGATCTGCGTGGTCATCGACCTGGACGAGACC
                    ************************************************************

HsCD00329760        CTGGTGCACAGCTCCTTCAAGCCAGTGAACAACGCGGACTTCATCATCCCTGTGGAGATT
NM_021198.2         CTGGTGCACAGCTCCTTCAAGCCAGTGAACAACGCGGACTTCATCATCCCTGTGGAGATT
NM_182642.2         CTGGTGCACAGCTCCTTCAAGCCAGTGAACAACGCGGACTTCATCATCCCTGTGGAGATT
NM_001206878.1      CTGGTGCACAGCTCCTTCAAGCCAGTGAACAACGCGGACTTCATCATCCCTGTGGAGATT
                    ************************************************************

HsCD00329760        GATGGGGTGGTCCACCAGGTCTACGTGTTGAAGCGTCCTCACGTGGATGAGTTCCTGCAG
NM_021198.2         GATGGGGTGGTCCACCAGGTCTACGTGTTGAAGCGTCCTCACGTGGATGAGTTCCTGCAG
NM_182642.2         GATGGGGTGGTCCACCAGGTCTACGTGTTGAAGCGTCCTCACGTGGATGAGTTCCTGCAG
NM_001206878.1      GATGGGGTGGTCCACCAGGTCTACGTGTTGAAGCGTCCTCACGTGGATGAGTTCCTGCAG
                    ************************************************************

HsCD00329760        CGAATGGGCGAGCTCTTTGAATGTGTGCTGTTCACTGCTAGCCTCGCCAAGTACGCAGAC
NM_021198.2         CGAATGGGCGAGCTCTTTGAATGTGTGCTGTTCACTGCTAGCCTCGCCAAGTACGCAGAC
NM_182642.2         CGAATGGGCGAGCTCTTTGAATGTGTGCTGTTCACTGCTAGCCTCGCCAAGTACGCAGAC
NM_001206878.1      CGAATGGGCGAGCTCTTTGAATGTGTGCTGTTCACTGCTAGCCTCGCCAAGTACGCAGAC
                    ************************************************************

HsCD00329760        CCAGTAGCTGACCTGCTGGACAAATGGGGGGCCTTCCGGGCCCGGCTGTTTCGAGAGTCC
NM_021198.2         CCAGTAGCTGACCTGCTGGACAAATGGGGGGCCTTCCGGGCCCGGCTGTTTCGAGAGTCC
NM_182642.2         CCAGTAGCTGACCTGCTGGACAAATGGGGGGCCTTCCGGGCCCGGCTGTTTCGAGAGTCC
NM_001206878.1      CCAGTAGCTGACCTGCTGGACAAATGGGGGGCCTTCCGGGCCCGGCTGTTTCGAGAGTCC
                    ************************************************************

HsCD00329760        TGCGTCTTCCACCGGGGGAACTACGTGAAGGACCTGAGCCGGTTGGGTCGAGACCTGCGG
NM_021198.2         TGCGTCTTCCACCGGGGGAACTACGTGAAGGACCTGAGCCGGTTGGGTCGAGACCTGCGG
NM_182642.2         TGCGTCTTCCACCGGGGGAACTACGTGAAGGACCTGAGCCGGTTGGGTCGAGACCTGCGG
NM_001206878.1      TGCGTCTTCCACCGGGGGAACTACGTGAAGGACCTGAGCCGGTTGGGTCGAGACCTGCGG
                    ************************************************************

HsCD00329760        CGGGTGCTCATCCTGGACAATTCACCTGCCTCCTATGTCTTCCATCCAGACAATGCTGTA
NM_021198.2         CGGGTGCTCATCCTGGACAATTCACCTGCCTCCTATGTCTTCCATCCAGACAATGCTGTA
NM_182642.2         CGGGTGCTCATCCTGGACAATTCACCTGCCTCCTATGTCTTCCATCCAGACAATGCTGTA
NM_001206878.1      CGGGTGCTCATCCTGGACAATTCACCTGCCTCCTATGTCTTCCATCCAGACAATGCTGTA
                    ************************************************************

HsCD00329760        CCGGTGGCCTCGTGGTTTGACAACATGAGTGACACAGAGCTCCACGACCTCCTCCCCTTC
NM_021198.2         CCGGTGGCCTCGTGGTTTGACAACATGAGTGACACAGAGCTCCACGACCTCCTCCCCTTC
NM_182642.2         CCGGTGGCCTCGTGGTTTGACAACATGAGTGACACAGAGCTCCACGACCTCCTCCCCTTC
NM_001206878.1      CCGGTGGCCTCGTGGTTTGACAACATGAGTGACACAGAGCTCCACGACCTCCTCCCCTTC
                    ************************************************************

HsCD00329760        TTCGAGCAACTCAGCCGTGTGGACGACGTGTACTCAGTGCTCAGGCAGCCACGGCCAGGG
NM_021198.2         TTCGAGCAACTCAGCCGTGTGGACGACGTGTACTCAGTGCTCAGGCAGCCACGGCCAGGG
NM_182642.2         TTCGAGCAACTCAGCCGTGTGGACGACGTGTACTCAGTGCTCAGGCAGCCACGGCCAGGG
NM_001206878.1      TTCGAGCAACTCAGCCGTGTGGACGACGTGTACTCAGTGCTCAGGCAGCCACGGCCAGGG
                    ************************************************************

HsCD00329760        AGCTTG
NM_021198.2         AGCTAG
NM_182642.2         AGCTAG
NM_001206878.1      AGCTAG
                    ****:*

Pathways

NCI : BMP receptor signaling
NCI : Coregulation of Androgen receptor activity
NCI : Regulation of cytoplasmic and nuclear SMAD2/3 signaling
WikiPathway : Epithelium TarBase
WikiPathway : Lymphocyte TarBase
WikiPathway : Muscle cell TarBase
WikiPathway : Squamous cell TarBase

Protein

SMART domain : CPDc : catalytic domain of ctd-like phosphatases
UniProt : Q9GZU7
UniProt : C9IYG0
HPRD : 05615

Clone

Cloning Information : 268501
HIP Master Clone ID : 193558
Original Clone ID : FLH268501.01L

Organism


TAX_ID : 9606
Species Specific ID: 58190

Genome

Chromosome : 2
Map Location : 2q35
Ensembl : ENSG00000144579

Reagents

Labome : CTDSP1-antibody