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PXMP3 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  PEX2
Gene Name:  peroxisomal biogenesis factor 2
Sequence             
Original Clone ID: FLH268505.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors:
HsCD00329764

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 918nts         Open reading frame : 1 to 918
Reference Sequence Alignment

ATGGCTTCCAGAAAAGAGAATGCGAAGAGTGCAAACAGAGTGCTAAGAATAAGCCAGTTG
GATGCACTTGAACTAAACAAGGCCCTGGAGCAGCTAGTTTGGTCCCAGTTTACTCAGTGC
TTTCATGGATTTAAACCTGGGCTGTTAGCTCGCTTTGAGCCAGAGGTGAAAGCGTGCTTA
TGGGTTTTCTTGTGGAGATTCACCATCTACTCCAAAAATGCCACAGTGGGACAGTCAGTT
TTGAATATTAAGTACAAAAATGATTTTTCCCCTAACCTGAGATATCAGCCACCCAGTAAA
AATCAAAAAATCTGGTATGCTGTTTGTACAATTGGTGGCAGGTGGTTAGAAGAACGATGC
TATGATTTGTTTCGAAACCATCATTTAGCATCATTTGGGAAAGTCAAGCAGTGTGTGAAT
TTTGTGATTGGACTTTTGAAATTAGGTGGGCTGATTAATTTTTTGATTTTCCTTCAGAGG
GGAAAGTTTGCAACTTTGACAGAACGTCTCCTAGGTATTCATTCTGTATTTTGCAAGCCT
CAAAACATACGTGAAGTTGGCTTTGAATACATGAATAGGGAACTTCTCTGGCATGGTTTT
GCTGAATTTCTGATTTTTCTCTTACCACTTATCAATGTCCAGAAGTTGAAAGCCAAGCTG
TCTTCATGGTGTATTCCTCTTACTGGTGCACCTAATAGTGACAATACATTAGCCACCAGT
GGCAAAGAATGCGCTCTATGTGGAGAGTGGCCCACCATGCCTCACACCATAGGATGTGAG
CATATTTTCTGTTATTTCTGTGCTAAGAGTAGTTTCTTATTTGACGTGTACTTTACTTGT
CCTAAGTGTGGCACAGAAGTACACAGTCTGCAGCCACTGAAATCAGGAATCGAGATGTCA
GCAGTAAATGCTCTTTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1145
Start on reference sequence 228
Mutations:
None
Discrepancy:
a902c,E301A
                                

5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : PEX2
Symbol Nomenclature : PEX2
SYNONYM : PAF1; PBD5A; PBD5B; PMP3; PMP35; PXMP3; RNF72; ZWS3
Designation : 35 kDa peroxisomal membrane protein|RING finger protein 72|peroxisomal membrane protein 3, 35kDa|peroxisome assembly factor 1|peroxisome assembly factor-1|peroxisome biogenesis factor 2
Full Nomenclature : peroxisomal biogenesis factor 2
GENEID : 5828
HGNC : 9717
MIM : 170993
Vega : OTTHUMG00000164530
Target GenBank: BC005375

 

Reference Sequence Alignment

  

HsCD00329764        ATGGCTTCCAGAAAAGAGAATGCGAAGAGTGCAAACAGAGTGCTAAGAATAAGCCAGTTG
NM_001172086.1      ATGGCTTCCAGAAAAGAGAATGCGAAGAGTGCAAACAGAGTGCTAAGAATAAGCCAGTTG
NM_001172087.1      ATGGCTTCCAGAAAAGAGAATGCGAAGAGTGCAAACAGAGTGCTAAGAATAAGCCAGTTG
NM_000318.2         ATGGCTTCCAGAAAAGAGAATGCGAAGAGTGCAAACAGAGTGCTAAGAATAAGCCAGTTG
NM_001079867.1      ATGGCTTCCAGAAAAGAGAATGCGAAGAGTGCAAACAGAGTGCTAAGAATAAGCCAGTTG
                    ************************************************************

HsCD00329764        GATGCACTTGAACTAAACAAGGCCCTGGAGCAGCTAGTTTGGTCCCAGTTTACTCAGTGC
NM_001172086.1      GATGCACTTGAACTAAACAAGGCCCTGGAGCAGCTAGTTTGGTCCCAGTTTACTCAGTGC
NM_001172087.1      GATGCACTTGAACTAAACAAGGCCCTGGAGCAGCTAGTTTGGTCCCAGTTTACTCAGTGC
NM_000318.2         GATGCACTTGAACTAAACAAGGCCCTGGAGCAGCTAGTTTGGTCCCAGTTTACTCAGTGC
NM_001079867.1      GATGCACTTGAACTAAACAAGGCCCTGGAGCAGCTAGTTTGGTCCCAGTTTACTCAGTGC
                    ************************************************************

HsCD00329764        TTTCATGGATTTAAACCTGGGCTGTTAGCTCGCTTTGAGCCAGAGGTGAAAGCGTGCTTA
NM_001172086.1      TTTCATGGATTTAAACCTGGGCTGTTAGCTCGCTTTGAGCCAGAGGTGAAAGCGTGCTTA
NM_001172087.1      TTTCATGGATTTAAACCTGGGCTGTTAGCTCGCTTTGAGCCAGAGGTGAAAGCGTGCTTA
NM_000318.2         TTTCATGGATTTAAACCTGGGCTGTTAGCTCGCTTTGAGCCAGAGGTGAAAGCGTGCTTA
NM_001079867.1      TTTCATGGATTTAAACCTGGGCTGTTAGCTCGCTTTGAGCCAGAGGTGAAAGCGTGCTTA
                    ************************************************************

HsCD00329764        TGGGTTTTCTTGTGGAGATTCACCATCTACTCCAAAAATGCCACAGTGGGACAGTCAGTT
NM_001172086.1      TGGGTTTTCTTGTGGAGATTCACCATCTACTCCAAAAATGCCACAGTGGGACAGTCAGTT
NM_001172087.1      TGGGTTTTCTTGTGGAGATTCACCATCTACTCCAAAAATGCCACAGTGGGACAGTCAGTT
NM_000318.2         TGGGTTTTCTTGTGGAGATTCACCATCTACTCCAAAAATGCCACAGTGGGACAGTCAGTT
NM_001079867.1      TGGGTTTTCTTGTGGAGATTCACCATCTACTCCAAAAATGCCACAGTGGGACAGTCAGTT
                    ************************************************************

HsCD00329764        TTGAATATTAAGTACAAAAATGATTTTTCCCCTAACCTGAGATATCAGCCACCCAGTAAA
NM_001172086.1      TTGAATATTAAGTACAAAAATGATTTTTCCCCTAACCTGAGATATCAGCCACCCAGTAAA
NM_001172087.1      TTGAATATTAAGTACAAAAATGATTTTTCCCCTAACCTGAGATATCAGCCACCCAGTAAA
NM_000318.2         TTGAATATTAAGTACAAAAATGATTTTTCCCCTAACCTGAGATATCAGCCACCCAGTAAA
NM_001079867.1      TTGAATATTAAGTACAAAAATGATTTTTCCCCTAACCTGAGATATCAGCCACCCAGTAAA
                    ************************************************************

HsCD00329764        AATCAAAAAATCTGGTATGCTGTTTGTACAATTGGTGGCAGGTGGTTAGAAGAACGATGC
NM_001172086.1      AATCAAAAAATCTGGTATGCTGTTTGTACAATTGGTGGCAGGTGGTTAGAAGAACGATGC
NM_001172087.1      AATCAAAAAATCTGGTATGCTGTTTGTACAATTGGTGGCAGGTGGTTAGAAGAACGATGC
NM_000318.2         AATCAAAAAATCTGGTATGCTGTTTGTACAATTGGTGGCAGGTGGTTAGAAGAACGATGC
NM_001079867.1      AATCAAAAAATCTGGTATGCTGTTTGTACAATTGGTGGCAGGTGGTTAGAAGAACGATGC
                    ************************************************************

HsCD00329764        TATGATTTGTTTCGAAACCATCATTTAGCATCATTTGGGAAAGTCAAGCAGTGTGTGAAT
NM_001172086.1      TATGATTTGTTTCGAAACCATCATTTAGCATCATTTGGGAAAGTCAAGCAGTGTGTGAAT
NM_001172087.1      TATGATTTGTTTCGAAACCATCATTTAGCATCATTTGGGAAAGTCAAGCAGTGTGTGAAT
NM_000318.2         TATGATTTGTTTCGAAACCATCATTTAGCATCATTTGGGAAAGTCAAGCAGTGTGTGAAT
NM_001079867.1      TATGATTTGTTTCGAAACCATCATTTAGCATCATTTGGGAAAGTCAAGCAGTGTGTGAAT
                    ************************************************************

HsCD00329764        TTTGTGATTGGACTTTTGAAATTAGGTGGGCTGATTAATTTTTTGATTTTCCTTCAGAGG
NM_001172086.1      TTTGTGATTGGACTTTTGAAATTAGGTGGGCTGATTAATTTTTTGATTTTCCTTCAGAGG
NM_001172087.1      TTTGTGATTGGACTTTTGAAATTAGGTGGGCTGATTAATTTTTTGATTTTCCTTCAGAGG
NM_000318.2         TTTGTGATTGGACTTTTGAAATTAGGTGGGCTGATTAATTTTTTGATTTTCCTTCAGAGG
NM_001079867.1      TTTGTGATTGGACTTTTGAAATTAGGTGGGCTGATTAATTTTTTGATTTTCCTTCAGAGG
                    ************************************************************

HsCD00329764        GGAAAGTTTGCAACTTTGACAGAACGTCTCCTAGGTATTCATTCTGTATTTTGCAAGCCT
NM_001172086.1      GGAAAGTTTGCAACTTTGACAGAACGTCTCCTAGGTATTCATTCTGTATTTTGCAAGCCT
NM_001172087.1      GGAAAGTTTGCAACTTTGACAGAACGTCTCCTAGGTATTCATTCTGTATTTTGCAAGCCT
NM_000318.2         GGAAAGTTTGCAACTTTGACAGAACGTCTCCTAGGTATTCATTCTGTATTTTGCAAGCCT
NM_001079867.1      GGAAAGTTTGCAACTTTGACAGAACGTCTCCTAGGTATTCATTCTGTATTTTGCAAGCCT
                    ************************************************************

HsCD00329764        CAAAACATACGTGAAGTTGGCTTTGAATACATGAATAGGGAACTTCTCTGGCATGGTTTT
NM_001172086.1      CAAAACATACGTGAAGTTGGCTTTGAATACATGAATAGGGAACTTCTCTGGCATGGTTTT
NM_001172087.1      CAAAACATACGTGAAGTTGGCTTTGAATACATGAATAGGGAACTTCTCTGGCATGGTTTT
NM_000318.2         CAAAACATACGTGAAGTTGGCTTTGAATACATGAATAGGGAACTTCTCTGGCATGGTTTT
NM_001079867.1      CAAAACATACGTGAAGTTGGCTTTGAATACATGAATAGGGAACTTCTCTGGCATGGTTTT
                    ************************************************************

HsCD00329764        GCTGAATTTCTGATTTTTCTCTTACCACTTATCAATGTCCAGAAGTTGAAAGCCAAGCTG
NM_001172086.1      GCTGAATTTCTGATTTTTCTCTTACCACTTATCAATGTCCAGAAGTTGAAAGCCAAGCTG
NM_001172087.1      GCTGAATTTCTGATTTTTCTCTTACCACTTATCAATGTCCAGAAGTTGAAAGCCAAGCTG
NM_000318.2         GCTGAATTTCTGATTTTTCTCTTACCACTTATCAATGTCCAGAAGTTGAAAGCCAAGCTG
NM_001079867.1      GCTGAATTTCTGATTTTTCTCTTACCACTTATCAATGTCCAGAAGTTGAAAGCCAAGCTG
                    ************************************************************

HsCD00329764        TCTTCATGGTGTATTCCTCTTACTGGTGCACCTAATAGTGACAATACATTAGCCACCAGT
NM_001172086.1      TCTTCATGGTGTATTCCTCTTACTGGTGCACCTAATAGTGACAATACATTAGCCACCAGT
NM_001172087.1      TCTTCATGGTGTATTCCTCTTACTGGTGCACCTAATAGTGACAATACATTAGCCACCAGT
NM_000318.2         TCTTCATGGTGTATTCCTCTTACTGGTGCACCTAATAGTGACAATACATTAGCCACCAGT
NM_001079867.1      TCTTCATGGTGTATTCCTCTTACTGGTGCACCTAATAGTGACAATACATTAGCCACCAGT
                    ************************************************************

HsCD00329764        GGCAAAGAATGCGCTCTATGTGGAGAGTGGCCCACCATGCCTCACACCATAGGATGTGAG
NM_001172086.1      GGCAAAGAATGCGCTCTATGTGGAGAGTGGCCCACCATGCCTCACACCATAGGATGTGAG
NM_001172087.1      GGCAAAGAATGCGCTCTATGTGGAGAGTGGCCCACCATGCCTCACACCATAGGATGTGAG
NM_000318.2         GGCAAAGAATGCGCTCTATGTGGAGAGTGGCCCACCATGCCTCACACCATAGGATGTGAG
NM_001079867.1      GGCAAAGAATGCGCTCTATGTGGAGAGTGGCCCACCATGCCTCACACCATAGGATGTGAG
                    ************************************************************

HsCD00329764        CATATTTTCTGTTATTTCTGTGCTAAGAGTAGTTTCTTATTTGACGTGTACTTTACTTGT
NM_001172086.1      CATATTTTCTGTTATTTCTGTGCTAAGAGTAGTTTCTTATTTGACGTGTACTTTACTTGT
NM_001172087.1      CATATTTTCTGTTATTTCTGTGCTAAGAGTAGTTTCTTATTTGACGTGTACTTTACTTGT
NM_000318.2         CATATTTTCTGTTATTTCTGTGCTAAGAGTAGTTTCTTATTTGACGTGTACTTTACTTGT
NM_001079867.1      CATATTTTCTGTTATTTCTGTGCTAAGAGTAGTTTCTTATTTGACGTGTACTTTACTTGT
                    ************************************************************

HsCD00329764        CCTAAGTGTGGCACAGAAGTACACAGTCTGCAGCCACTGAAATCAGGAATCGAGATGTCA
NM_001172086.1      CCTAAGTGTGGCACAGAAGTACACAGTCTGCAGCCACTGAAATCAGGAATCGAGATGTCA
NM_001172087.1      CCTAAGTGTGGCACAGAAGTACACAGTCTGCAGCCACTGAAATCAGGAATCGAGATGTCA
NM_000318.2         CCTAAGTGTGGCACAGAAGTACACAGTCTGCAGCCACTGAAATCAGGAATCGAGATGTCA
NM_001079867.1      CCTAAGTGTGGCACAGAAGTACACAGTCTGCAGCCACTGAAATCAGGAATCGAGATGTCA
                    ************************************************************

HsCD00329764        GCAGTAAATGCTCTTTTG
NM_001172086.1      GAAGTAAATGCTCTTTAG
NM_001172087.1      GAAGTAAATGCTCTTTAG
NM_000318.2         GAAGTAAATGCTCTTTAG
NM_001079867.1      GAAGTAAATGCTCTTTAG
                    *.**************:*

Protein

SMART domain : RING : Ring finger
UniProt : P28328
HPRD : 01367

Clone

Cloning Information : 268505
HIP Master Clone ID : 193570
Original Clone ID : FLH268505.01L

Organism


TAX_ID : 9606
Species Specific ID: 5828

Genome

Chromosome : 8
Map Location : 8q21.1
Ensembl : ENSG00000164751

Reagents

Labome : PEX2-antibody