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GATAD2B (H. sapiens ) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  GATAD2B
Gene Name:  GATA zinc finger domain containing 2B
Sequence             
Original Clone ID: FLH268506.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329765

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1782nts         Open reading frame : 1 to 1782
Reference Sequence Alignment

ATGGATAGAATGACAGAAGATGCTCTTCGCTTGAATCTGTTGAAGCGGAGCTTGGACCCA
GCAGATGAGCGAGATGATGTCCTGGCAAAGCGACTCAAAATGGAGGGGCATGAGGCCATG
GAACGTCTGAAAATGTTGGCATTGCTCAAAAGGAAGGATTTGGCAAATCTTGAGGTGCCA
CATGAGTTACCCACCAAACAGGATGGCAGTGGTGTCAAGGGCTATGAAGAAAAACTTAAC
GGGAATCTCAGGCCTCATGGAGACAACAGGACTGCTGGAAGGCCAGGCAAAGAAAACATC
AATGATGAGCCTGTGGATATGAGTGCTAGACGGAGTGAGCCAGAGCGAGGAAGGCTAACT
CCCTCACCAGACATCATTGTTTTGTCTGACAATGAGGCTTCCAGTCCCCGTTCCAGTTCC
AGAATGGAAGAAAGACTCAAAGCAGCCAACTTAGAGATGTTTAAGGGGAAAGGCATTGAG
GAGCGGCAGCAGCTTATCAAGCAGCTGAGGGATGAGCTACGATTGGAAGAAGCCCGACTG
GTCCTGTTAAAGAAACTGAGACAGAGTCAGCTACAGAAAGAGAATGTGGTCCAGAAGACT
CCAGTTGTACAGAATGCAGCATCTATTGTTCAGCCATCTCCTGCCCATGTGGGACAGCAG
GGCCTATCTAAGCTTCCCTCTCGGCCTGGGGCCCAAGGGGTTGAACCTCAAAATTTGAGA
ACATTACAGGGTCACAGTGTCATCCGTTCAGCTACCAATACCACCCTTCCACACATGTTG
ATGTCTCAACGTGTTATTGCACCAAACCCAGCCCAGCTACAGGGTCAGCGGGGCCCGCCT
AAGCCTGGCCTTGTACGCACCACAACACCCAACATGAATCCCGCCATCAATTATCAACCG
CAGTCAAGTTCTTCTGTTCCATGTCAGCGTACAACATCCTCTGCCATCTATATGAACCTT
GCTTCTCATATCCAGCCAGGGACGGTGAACAGAGTGTCCTCGCCACTTCCTAGCCCCAGC
GCCATGACTGATGCTGCCAACTCACAGGCTGCAGCCAAATTGGCTCTTCGCAAACAGCTG
GAAAAGACACTCCTGGAGATCCCACCCCCTAAACCTCCTGCTCCCTTACTTCATTTCTTG
CCTAGTGCAGCCAATAGCGAGTTCATCTACATGGTAGGCTTGGAAGAAGTCGTACAGAGT
GTCATTGACAGCCAAGGCAAAAGCTGTGCCTCACTTCTGCGGGTTGAACCCTTTGTATGT
GCCCAGTGCCGCACAGATTTCACCCCTCACTGGAAGCAAGAAAAGAATGGTAAGATTCTA
TGTGAGCAGTGTATGACCTCCAACCAGAAAAAGGCTCTAAAAGCTGAACACACCAACCGG
CTGAAAAATGCATTTGTGAAAGCCCTACAGCAGGAACAGGAAATTGAACAGCGATTACAG
CAGCAGGCAGCCCTCTCCCCCACTACGGCTCCAGCTGTGTCCAGTGTCAGTAAACAAGAG
ACCATCATGAGACATCATACGCTTCGGCAGGCTCCACAGCCCCAGAGCAGCCTCCAGCGT
GGCATACCCACATCTGCCCGCTCCATGCTTTCAAACTTTGCACAGGCACCCCAGTTGTCT
GTGCCAGGTGGCCTCCTTGGTATGCCAGGTGTCAACATTGCATACTTGAATACTGGCATC
GGAGGACACAAAGGCCCCAGTTTGGCAGACCGACAGCGTGAATACCTTTTAGACATGATC
CCTCCCCGGTCTATATCGCAGTCCATCAGTGGACAGAAATTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1799
Start on reference sequence 18
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : GATAD2B
Symbol Nomenclature : GATAD2B
SYNONYM : MRD18; P66beta; RP11-216N14.6; p68
Designation : GATA zinc finger domain-containing protein 2B|p66/p68|transcription repressor p66 beta component of the MeCP1 complex|transcriptional repressor p66-beta
Full Nomenclature : GATA zinc finger domain containing 2B
GENEID : 57459
HGNC : 30778
MIM : 614998
Vega : OTTHUMG00000037162
Target GenBank: BC069419

 

Reference Sequence Alignment

  

HsCD00329765      ATGGATAGAATGACAGAAGATGCTCTTCGCTTGAATCTGTTGAAGCGGAGCTTGGACCCA
NM_020699.2       ATGGATAGAATGACAGAAGATGCTCTTCGCTTGAATCTGTTGAAGCGGAGCTTGGACCCA
                  ************************************************************

HsCD00329765      GCAGATGAGCGAGATGATGTCCTGGCAAAGCGACTCAAAATGGAGGGGCATGAGGCCATG
NM_020699.2       GCAGATGAGCGAGATGATGTCCTGGCAAAGCGACTCAAAATGGAGGGGCATGAGGCCATG
                  ************************************************************

HsCD00329765      GAACGTCTGAAAATGTTGGCATTGCTCAAAAGGAAGGATTTGGCAAATCTTGAGGTGCCA
NM_020699.2       GAACGTCTGAAAATGTTGGCATTGCTCAAAAGGAAGGATTTGGCAAATCTTGAGGTGCCA
                  ************************************************************

HsCD00329765      CATGAGTTACCCACCAAACAGGATGGCAGTGGTGTCAAGGGCTATGAAGAAAAACTTAAC
NM_020699.2       CATGAGTTACCCACCAAACAGGATGGCAGTGGTGTCAAGGGCTATGAAGAAAAACTTAAC
                  ************************************************************

HsCD00329765      GGGAATCTCAGGCCTCATGGAGACAACAGGACTGCTGGAAGGCCAGGCAAAGAAAACATC
NM_020699.2       GGGAATCTCAGGCCTCATGGAGACAACAGGACTGCTGGAAGGCCAGGCAAAGAAAACATC
                  ************************************************************

HsCD00329765      AATGATGAGCCTGTGGATATGAGTGCTAGACGGAGTGAGCCAGAGCGAGGAAGGCTAACT
NM_020699.2       AATGATGAGCCTGTGGATATGAGTGCTAGACGGAGTGAGCCAGAGCGAGGAAGGCTAACT
                  ************************************************************

HsCD00329765      CCCTCACCAGACATCATTGTTTTGTCTGACAATGAGGCTTCCAGTCCCCGTTCCAGTTCC
NM_020699.2       CCCTCACCAGACATCATTGTTTTGTCTGACAATGAGGCTTCCAGTCCCCGTTCCAGTTCC
                  ************************************************************

HsCD00329765      AGAATGGAAGAAAGACTCAAAGCAGCCAACTTAGAGATGTTTAAGGGGAAAGGCATTGAG
NM_020699.2       AGAATGGAAGAAAGACTCAAAGCAGCCAACTTAGAGATGTTTAAGGGGAAAGGCATTGAG
                  ************************************************************

HsCD00329765      GAGCGGCAGCAGCTTATCAAGCAGCTGAGGGATGAGCTACGATTGGAAGAAGCCCGACTG
NM_020699.2       GAGCGGCAGCAGCTTATCAAGCAGCTGAGGGATGAGCTACGATTGGAAGAAGCCCGACTG
                  ************************************************************

HsCD00329765      GTCCTGTTAAAGAAACTGAGACAGAGTCAGCTACAGAAAGAGAATGTGGTCCAGAAGACT
NM_020699.2       GTCCTGTTAAAGAAACTGAGACAGAGTCAGCTACAGAAAGAGAATGTGGTCCAGAAGACT
                  ************************************************************

HsCD00329765      CCAGTTGTACAGAATGCAGCATCTATTGTTCAGCCATCTCCTGCCCATGTGGGACAGCAG
NM_020699.2       CCAGTTGTACAGAATGCAGCATCTATTGTTCAGCCATCTCCTGCCCATGTGGGACAGCAG
                  ************************************************************

HsCD00329765      GGCCTATCTAAGCTTCCCTCTCGGCCTGGGGCCCAAGGGGTTGAACCTCAAAATTTGAGA
NM_020699.2       GGCCTATCTAAGCTTCCCTCTCGGCCTGGGGCCCAAGGGGTTGAACCTCAAAATTTGAGA
                  ************************************************************

HsCD00329765      ACATTACAGGGTCACAGTGTCATCCGTTCAGCTACCAATACCACCCTTCCACACATGTTG
NM_020699.2       ACATTACAGGGTCACAGTGTCATCCGTTCAGCTACCAATACCACCCTTCCACACATGTTG
                  ************************************************************

HsCD00329765      ATGTCTCAACGTGTTATTGCACCAAACCCAGCCCAGCTACAGGGTCAGCGGGGCCCGCCT
NM_020699.2       ATGTCTCAACGTGTTATTGCACCAAACCCAGCCCAGCTACAGGGTCAGCGGGGCCCGCCT
                  ************************************************************

HsCD00329765      AAGCCTGGCCTTGTACGCACCACAACACCCAACATGAATCCCGCCATCAATTATCAACCG
NM_020699.2       AAGCCTGGCCTTGTACGCACCACAACACCCAACATGAATCCCGCCATCAATTATCAACCG
                  ************************************************************

HsCD00329765      CAGTCAAGTTCTTCTGTTCCATGTCAGCGTACAACATCCTCTGCCATCTATATGAACCTT
NM_020699.2       CAGTCAAGTTCTTCTGTTCCATGTCAGCGTACAACATCCTCTGCCATCTATATGAACCTT
                  ************************************************************

HsCD00329765      GCTTCTCATATCCAGCCAGGGACGGTGAACAGAGTGTCCTCGCCACTTCCTAGCCCCAGC
NM_020699.2       GCTTCTCATATCCAGCCAGGGACGGTGAACAGAGTGTCCTCGCCACTTCCTAGCCCCAGC
                  ************************************************************

HsCD00329765      GCCATGACTGATGCTGCCAACTCACAGGCTGCAGCCAAATTGGCTCTTCGCAAACAGCTG
NM_020699.2       GCCATGACTGATGCTGCCAACTCACAGGCTGCAGCCAAATTGGCTCTTCGCAAACAGCTG
                  ************************************************************

HsCD00329765      GAAAAGACACTCCTGGAGATCCCACCCCCTAAACCTCCTGCTCCCTTACTTCATTTCTTG
NM_020699.2       GAAAAGACACTCCTGGAGATCCCACCCCCTAAACCTCCTGCTCCCTTACTTCATTTCTTG
                  ************************************************************

HsCD00329765      CCTAGTGCAGCCAATAGCGAGTTCATCTACATGGTAGGCTTGGAAGAAGTCGTACAGAGT
NM_020699.2       CCTAGTGCAGCCAATAGCGAGTTCATCTACATGGTAGGCTTGGAAGAAGTCGTACAGAGT
                  ************************************************************

HsCD00329765      GTCATTGACAGCCAAGGCAAAAGCTGTGCCTCACTTCTGCGGGTTGAACCCTTTGTATGT
NM_020699.2       GTCATTGACAGCCAAGGCAAAAGCTGTGCCTCACTTCTGCGGGTTGAACCCTTTGTATGT
                  ************************************************************

HsCD00329765      GCCCAGTGCCGCACAGATTTCACCCCTCACTGGAAGCAAGAAAAGAATGGTAAGATTCTA
NM_020699.2       GCCCAGTGCCGCACAGATTTCACCCCTCACTGGAAGCAAGAAAAGAATGGTAAGATTCTA
                  ************************************************************

HsCD00329765      TGTGAGCAGTGTATGACCTCCAACCAGAAAAAGGCTCTAAAAGCTGAACACACCAACCGG
NM_020699.2       TGTGAGCAGTGTATGACCTCCAACCAGAAAAAGGCTCTAAAAGCTGAACACACCAACCGG
                  ************************************************************

HsCD00329765      CTGAAAAATGCATTTGTGAAAGCCCTACAGCAGGAACAGGAAATTGAACAGCGATTACAG
NM_020699.2       CTGAAAAATGCATTTGTGAAAGCCCTACAGCAGGAACAGGAAATTGAACAGCGATTACAG
                  ************************************************************

HsCD00329765      CAGCAGGCAGCCCTCTCCCCCACTACGGCTCCAGCTGTGTCCAGTGTCAGTAAACAAGAG
NM_020699.2       CAGCAGGCAGCCCTCTCCCCCACTACGGCTCCAGCTGTGTCCAGTGTCAGTAAACAAGAG
                  ************************************************************

HsCD00329765      ACCATCATGAGACATCATACGCTTCGGCAGGCTCCACAGCCCCAGAGCAGCCTCCAGCGT
NM_020699.2       ACCATCATGAGACATCATACGCTTCGGCAGGCTCCACAGCCCCAGAGCAGCCTCCAGCGT
                  ************************************************************

HsCD00329765      GGCATACCCACATCTGCCCGCTCCATGCTTTCAAACTTTGCACAGGCACCCCAGTTGTCT
NM_020699.2       GGCATACCCACATCTGCCCGCTCCATGCTTTCAAACTTTGCACAGGCACCCCAGTTGTCT
                  ************************************************************

HsCD00329765      GTGCCAGGTGGCCTCCTTGGTATGCCAGGTGTCAACATTGCATACTTGAATACTGGCATC
NM_020699.2       GTGCCAGGTGGCCTCCTTGGTATGCCAGGTGTCAACATTGCATACTTGAATACTGGCATC
                  ************************************************************

HsCD00329765      GGAGGACACAAAGGCCCCAGTTTGGCAGACCGACAGCGTGAATACCTTTTAGACATGATC
NM_020699.2       GGAGGACACAAAGGCCCCAGTTTGGCAGACCGACAGCGTGAATACCTTTTAGACATGATC
                  ************************************************************

HsCD00329765      CCTCCCCGGTCTATATCGCAGTCCATCAGTGGACAGAAATTG
NM_020699.2       CCTCCCCGGTCTATATCGCAGTCCATCAGTGGACAGAAATAA
                  ****************************************:.

Pathways

NCI : Signaling events mediated by HDAC Class I
Reactome : Gene Expression
Reactome : RNA Polymerase I Promoter Clearance
Reactome : RNA Polymerase I Transcription
Reactome : RNA Polymerase I Transcription Initiation
Reactome : RNA Polymerase I, RNA Polymerase III, and Mitochondrial Transcription
Reactome : Transcription

Protein

UniProt : Q8WXI9
HPRD : 15094

Clone

Cloning Information : 268506
HIP Master Clone ID : 193571
Original Clone ID : FLH268506.01L

Organism


TAX_ID : 9606
Species Specific ID: 57459

Genome

Chromosome : 1
Map Location : 1q21.3
Ensembl : ENSG00000143614

Reagents

Labome : GATAD2B-antibody