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ZNF571 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  ZNF571
Gene Name:  zinc finger protein 571
Sequence             
Original Clone ID: FLH268507.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329766

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1830nts         Open reading frame : 1 to 1830
Reference Sequence Alignment

ATGCCCCACTTGTTGGTGACTTTCAGGGATGTGGCCATTGACTTCTCTCAGGAGGAATGG
GAATGCCTGGACCCTGCTCAGAGGGACTTGTACAGGGATGTGATGTTGGAGAACTACAGC
AACCTGATCTCATTGGACTTGGAATCCAGTTGTGTGACCAAAAAGTTATCTCCAGAAAAG
GAAATTTATGAAATGGAATCACTCCAGTGGGAGAATATGGGGAAACGTATCAACCATCAC
CTTCAATACAATGGTCTTGGAGACAATATGGAGTGCAAAGGCAACTTAGAGGGTCAAGAA
GCAAATCAGGAAGGGCTTTACATGTGTGTCAAAATTACCTGTGAAGAAAAGGCCACTGAA
AGTCATTCAACCTCTTCTACTTTTCATCGGATAATTCCTACCAAGGAAAAATTGTACAAA
TGTAAGGAATGCAGACAAGGTTTCAGCTACCTGTCATGCCTTATTCAACATGAGGAAAAT
CATAATATAGAAAAATGCTCTGAAGTTAAGAAACACAGGAATACCTTTAGCAAAAAGCCA
AGCTATATTCAACATCAGAGAATTCACACTGGTGAGAAACCTTATGAGTGTATGGAATGT
GGAAAGGCCTTTGGTCGTACTTCTGATCTCATTCAACATCAGAAAATTCATACTAATGAA
AAACCTTATCAGTGTAACGCATGTGGGAAAGCTTTTATTCGTGGTTCACAGCTCACTGAA
CATCAGAGAGTTCATACAGGAGAGAAACCATATGAATGTAAGAAATGTGGAAAAGCCTTT
AGTTATTGTTCACAATATACTCTTCATCAGAGAATTCATAGTGGTGAAAAACCCTATGAA
TGTAAGGATTGTGGGAAGGCCTTTATTCTTGGCTCTCAACTTACTTACCATCAGAGAATT
CATAGTGGTGAGAAACCTTATGAGTGTAAGGAATGTGGAAAGGCCTTTATTCTTGGTTCA
CACCTTACATACCATCAGAGAGTTCATACTGGTGAAAAGCCTTACATATGTAAAGAATGT
GGGAAAGCCTTTTTATGTGCCTCCCAACTGAATGAACATCAGAGAATTCATACAGGAGAG
AAACCCTATGAATGTAAAGAATGCGGGAAGACCTTTTTTCGTGGCTCACAACTTACTTAC
CACCTGAGAGTTCATTCAGGTGAGAGACCTTATAAATGCAAAGAATGTGGGAAAGCCTTT
ATTTCTAATTCTAATCTTATTCAACATCAAAGAATTCATACCGGAGAGAAGCCCTACAAA
TGTAAGGAATGTGGAAAGGCCTTTATTTGTGGCAAACAACTTAGTGAACATCAGAGAATT
CATACAGGTGAGAAACCCTTTGAATGTAAGGAATGTGGAAAGGCCTTTATTCGTGTTGCA
TATCTTACTCAACATGAGAAAATTCATGGTGAGAAACATTATGAATGTAAGGAATGTGGG
AAGACCTTTGTACGTGCTACACAACTTACATATCATCAAAGAATTCATACAGGTGAAAAG
CCCTACAAATGTAAGGAATGTGACAAGGCCTTTATTTATGGCTCACAACTTAGTGAACAT
CAGAGAATTCACAGAGGTGAAAAACCTTATGAATGTAAACAGTGTGGGAAGGCTTTTATT
CGTGGCTCACACCTTACTGAACATCTGAGAACTCATACTGGAGAGAAACCCTATGAATGT
AAGGAATGTGGGAGGGCCTTTAGTCGTGGCTCAGAACTTACTCTGCATCAAAGGATCCAT
ACTGGTGAGAAACCCTATACATGTGTCCAGTGTGGTAAAGACTTTAGATGTCCTTCACAA
CTTACTCAACATACAAGGCTTCATAATTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 2559
Start on reference sequence 730
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ZNF571
Symbol Nomenclature : ZNF571
Full Nomenclature : zinc finger protein 571
GENEID : 51276
Locus Tag : HSPC059
HGNC : 25000
Vega : OTTHUMG00000182016
Target GenBank: BC040591

 

Reference Sequence Alignment

  

HsCD00329766      ATGCCCCACTTGTTGGTGACTTTCAGGGATGTGGCCATTGACTTCTCTCAGGAGGAATGG
NM_016536.3       ATGCCCCACTTGTTGGTGACTTTCAGGGATGTGGCCATTGACTTCTCTCAGGAGGAATGG
                  ************************************************************

HsCD00329766      GAATGCCTGGACCCTGCTCAGAGGGACTTGTACAGGGATGTGATGTTGGAGAACTACAGC
NM_016536.3       GAATGCCTGGACCCTGCTCAGAGGGACTTGTACAGGGATGTGATGTTGGAGAACTACAGC
                  ************************************************************

HsCD00329766      AACCTGATCTCATTGGACTTGGAATCCAGTTGTGTGACCAAAAAGTTATCTCCAGAAAAG
NM_016536.3       AACCTGATCTCATTGGACTTGGAATCCAGTTGTGTGACCAAAAAGTTATCTCCAGAAAAG
                  ************************************************************

HsCD00329766      GAAATTTATGAAATGGAATCACTCCAGTGGGAGAATATGGGGAAACGTATCAACCATCAC
NM_016536.3       GAAATTTATGAAATGGAATCACTCCAGTGGGAGAATATGGGGAAACGTATCAACCATCAC
                  ************************************************************

HsCD00329766      CTTCAATACAATGGTCTTGGAGACAATATGGAGTGCAAAGGCAACTTAGAGGGTCAAGAA
NM_016536.3       CTTCAATACAATGGTCTTGGAGACAATATGGAGTGCAAAGGCAACTTAGAGGGTCAAGAA
                  ************************************************************

HsCD00329766      GCAAATCAGGAAGGGCTTTACATGTGTGTCAAAATTACCTGTGAAGAAAAGGCCACTGAA
NM_016536.3       GCAAGTCAGGAAGGGCTTTACATGTGTGTCAAAATTACCTGTGAAGAAAAGGCCACTGAA
                  ****.*******************************************************

HsCD00329766      AGTCATTCAACCTCTTCTACTTTTCATCGGATAATTCCTACCAAGGAAAAATTGTACAAA
NM_016536.3       AGTCATTCAACCTCTTCTACTTTTCATCGGATAATTCCTACCAAGGAAAAATTGTACAAA
                  ************************************************************

HsCD00329766      TGTAAGGAATGCAGACAAGGTTTCAGCTACCTGTCATGCCTTATTCAACATGAGGAAAAT
NM_016536.3       TGTAAGGAATGCAGACAAGGTTTCAGCTACCTGTCATGCCTTATTCAACATGAGGAAAAT
                  ************************************************************

HsCD00329766      CATAATATAGAAAAATGCTCTGAAGTTAAGAAACACAGGAATACCTTTAGCAAAAAGCCA
NM_016536.3       CATAATATAGAAAAATGCTCTGAAGTTAAGAAACACAGGAATACCTTTAGCAAAAAGCCA
                  ************************************************************

HsCD00329766      AGCTATATTCAACATCAGAGAATTCACACTGGTGAGAAACCTTATGAGTGTATGGAATGT
NM_016536.3       AGCTATATTCAACATCAGAGAATTCAGACTGGTGAGAAACCTTATGAGTGTATGGAATGT
                  ************************** *********************************

HsCD00329766      GGAAAGGCCTTTGGTCGTACTTCTGATCTCATTCAACATCAGAAAATTCATACTAATGAA
NM_016536.3       GGAAAGGCCTTTGGTCGTACTTCTGATCTCATTCAACATCAGAAAATTCATACTAATGAA
                  ************************************************************

HsCD00329766      AAACCTTATCAGTGTAACGCATGTGGGAAAGCTTTTATTCGTGGTTCACAGCTCACTGAA
NM_016536.3       AAACCTTATCAGTGTAACGCATGTGGGAAAGCTTTTATTCGTGGTTCACAGCTCACTGAA
                  ************************************************************

HsCD00329766      CATCAGAGAGTTCATACAGGAGAGAAACCATATGAATGTAAGAAATGTGGAAAAGCCTTT
NM_016536.3       CATCAGAGAGTTCATACAGGAGAGAAACCATATGAATGTAAGAAATGTGGAAAAGCCTTT
                  ************************************************************

HsCD00329766      AGTTATTGTTCACAATATACTCTTCATCAGAGAATTCATAGTGGTGAAAAACCCTATGAA
NM_016536.3       AGTTATTGTTCACAATATACTCTTCATCAGAGAATTCATAGTGGTGAAAAACCCTATGAA
                  ************************************************************

HsCD00329766      TGTAAGGATTGTGGGAAGGCCTTTATTCTTGGCTCTCAACTTACTTACCATCAGAGAATT
NM_016536.3       TGTAAGGATTGTGGGAAGGCCTTTATTCTTGGCTCTCAACTTACTTACCATCAGAGAATT
                  ************************************************************

HsCD00329766      CATAGTGGTGAGAAACCTTATGAGTGTAAGGAATGTGGAAAGGCCTTTATTCTTGGTTCA
NM_016536.3       CATAGTGGTGAGAAACCTTATGAGTGTAAGGAATGTGGAAAGGCCTTTATTCTTGGTTCA
                  ************************************************************

HsCD00329766      CACCTTACATACCATCAGAGAGTTCATACTGGTGAAAAGCCTTACATATGTAAAGAATGT
NM_016536.3       CACCTTACATACCATCAGAGAGTTCATACTGGTGAAAAGCCTTACATATGTAAAGAATGT
                  ************************************************************

HsCD00329766      GGGAAAGCCTTTTTATGTGCCTCCCAACTGAATGAACATCAGAGAATTCATACAGGAGAG
NM_016536.3       GGGAAAGCCTTTTTATGTGCCTCCCAACTGAATGAACATCAGAGAATTCATACAGGAGAG
                  ************************************************************

HsCD00329766      AAACCCTATGAATGTAAAGAATGCGGGAAGACCTTTTTTCGTGGCTCACAACTTACTTAC
NM_016536.3       AAACCCTATGAATGTAAAGAATGCGGGAAGACCTTTTTTCGTGGCTCACAACTTACTTAC
                  ************************************************************

HsCD00329766      CACCTGAGAGTTCATTCAGGTGAGAGACCTTATAAATGCAAAGAATGTGGGAAAGCCTTT
NM_016536.3       CACCTGAGAGTTCATTCAGGTGAGAGACCTTATAAATGCAAAGAATGTGGGAAAGCCTTT
                  ************************************************************

HsCD00329766      ATTTCTAATTCTAATCTTATTCAACATCAAAGAATTCATACCGGAGAGAAGCCCTACAAA
NM_016536.3       ATTTCTAATTCTAATCTTATTCAACATCAAAGAATTCATACCGGAGAGAAGCCCTACAAA
                  ************************************************************

HsCD00329766      TGTAAGGAATGTGGAAAGGCCTTTATTTGTGGCAAACAACTTAGTGAACATCAGAGAATT
NM_016536.3       TGTAAGGAATGTGGAAAGGCCTTTATTTGTGGCAAACAACTTAGTGAACATCAGAGAATT
                  ************************************************************

HsCD00329766      CATACAGGTGAGAAACCCTTTGAATGTAAGGAATGTGGAAAGGCCTTTATTCGTGTTGCA
NM_016536.3       CATACAGGTGAGAAACCCTTTGAATGTAAGGAATGTGGAAAGGCCTTTATTCGTGTTGCA
                  ************************************************************

HsCD00329766      TATCTTACTCAACATGAGAAAATTCATGGTGAGAAACATTATGAATGTAAGGAATGTGGG
NM_016536.3       TATCTTACTCAACATGAGAAAATTCATGGTGAGAAACATTATGAATGTAAGGAATGTGGG
                  ************************************************************

HsCD00329766      AAGACCTTTGTACGTGCTACACAACTTACATATCATCAAAGAATTCATACAGGTGAAAAG
NM_016536.3       AAGACCTTTGTACGTGCTACACAACTTACATATCATCAAAGAATTCATACAGGTGAAAAG
                  ************************************************************

HsCD00329766      CCCTACAAATGTAAGGAATGTGACAAGGCCTTTATTTATGGCTCACAACTTAGTGAACAT
NM_016536.3       CCCTACAAATGTAAGGAATGTGACAAGGCCTTTATTTATGGCTCACAACTTAGTGAACAT
                  ************************************************************

HsCD00329766      CAGAGAATTCACAGAGGTGAAAAACCTTATGAATGTAAACAGTGTGGGAAGGCTTTTATT
NM_016536.3       CAGAGAATTCACAGAGGTGAAAAACCTTATGAATGTAAACAGTGTGGGAAGGCTTTTATT
                  ************************************************************

HsCD00329766      CGTGGCTCACACCTTACTGAACATCTGAGAACTCATACTGGAGAGAAACCCTATGAATGT
NM_016536.3       CGTGGCTCACACCTTACTGAACATCTGAGAACTCATACTGGAGAGAAACCCTATGAATGT
                  ************************************************************

HsCD00329766      AAGGAATGTGGGAGGGCCTTTAGTCGTGGCTCAGAACTTACTCTGCATCAAAGGATCCAT
NM_016536.3       AAGGAATGTGGGAGGGCCTTTAGTCGTGGCTCAGAACTTACTCTGCATCAAAGGATCCAT
                  ************************************************************

HsCD00329766      ACTGGTGAGAAACCCTATACATGTGTCCAGTGTGGTAAAGACTTTAGATGTCCTTCACAA
NM_016536.3       ACTGGTGAGAAACCCTATACATGTGTCCAGTGTGGTAAAGACTTTAGATGTCCTTCACAA
                  ************************************************************

HsCD00329766      CTTACTCAACATACAAGGCTTCATAATTTG
NM_016536.3       CTTACTCAACATACAAGGCTTCATAATTGA
                  **************************** .

Pathways

Reactome : Gene Expression
Reactome : Generic Transcription Pathway

Protein

SMART domain : ZnF_C2H2 : zinc finger
SMART domain : KRAB : krueppel associated box
UniProt : Q7Z3V5
HPRD : 15850

Clone

Cloning Information : 268507
HIP Master Clone ID : 193577
Original Clone ID : FLH268507.01L

Organism


TAX_ID : 9606
Species Specific ID: 51276

Genome

Chromosome : 19
Map Location : 19q13.12
Ensembl : ENSG00000180479

Reagents

Labome : ZNF571-antibody