DNASU Plasmid Repository • 480.965.5697 | Email

STAF42 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  TADA1
Gene Name:  transcriptional adaptor 1
Sequence             
Original Clone ID: FLH268509.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329768

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1008nts         Open reading frame : 1 to 1008
Reference Sequence Alignment

ATGGCGACCTTTGTGAGCGAGCTGGAGGCGGCCAAGAAGAACTTAAGCGAGGCCCTGGGG
GACAACGTGAAACAATACTGGGCTAACCTAAAGCTGTGGTTCAAGCAGAAGATCAGCAAA
GAGGAGTTTGACCTTGAAGCTCATAGACTTCTCACACAGGATAATGTCCATTCTCACAAT
GATTTCCTCCTGGCCATTCTCACGCGTTGTCAGATTTTGGTTTCTACACCAGATGGTGCT
GGATCTTTGCCTTGGCCAGGGGGTTCCGCAGCAAAACCTGGAAAACCCAAGGGAAAGAAA
AAGCTTTCTTCTGTTCGTCAGAAATTTGATCATAGATTCCAGCCTCAAAATCCTCTCTCA
GGAGCCCAGCAATTTGTGGCAAAGGATCCCCAAGATGATGACGACTTGAAACTTTGTTCC
CACACAATGATGCTTCCCACTCGAGGCCAGCTTGAAGGGAGAATGATAGTGACTGCTTAT
GAGCATGGGCTGGACAATGTCACCGAGGAGGCTGTTTCAGCTGTTGTCTATGCTGTGGAG
AATCACCTTAAAGATATACTGACGTCAGTTGTGTCAAGAAGGAAAGCTTATCGGTTACGA
GATGGTCATTTTAAATATGCCTTTGGCAGTAACGTGACCCCGCAGCCATACCTGAAGAAT
AGTGTAGTAGCTTACAACAACTTAATAGAAAGCCCTCCAGCTTTTACTGCTCCCTGTGCT
GGTCAGAATCCAGCTTCTCACCCACCCCCTGATGATGCTGAGCAGCAGGCTGCACTCCTG
CTGGCATGCTCCGGAGACACTCTACCTGCATCTTTGCCTCCGGTGAACATGTACGATCTT
TTTGAAGCTTTGCAGGTGCACAGGGAAGTCATCCCTACACATACTGTCTATGCTCTTAAC
ATTGAAAGGATCATCACGAAACTCTGGCATCCAAATCATGAAGAGCTGCAGCAAGACAAA
GTTCACCGCCAGCGCTTGGCAGCCAAGGAGGGGCTTTTGCTGTGCTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1029
Start on reference sequence 22
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : TADA1
Symbol Nomenclature : TADA1
SYNONYM : ADA1; HFI1; RP1-9E21.4; STAF42; TADA1L; hADA1
Designation : SPT3-associated factor 42 (STAF42)|transcriptional adapter 1|transcriptional adapter 1-like protein|transcriptional adaptor 1 (HFI1 homolog, yeast)
Full Nomenclature : transcriptional adaptor 1
GENEID : 117143
HGNC : 30631
MIM : 612763
Vega : OTTHUMG00000034321
Target GenBank: BC015401

 

Reference Sequence Alignment

  

HsCD00329768      ATGGCGACCTTTGTGAGCGAGCTGGAGGCGGCCAAGAAGAACTTAAGCGAGGCCCTGGGG
NM_053053.3       ATGGCGACCTTTGTGAGCGAGCTGGAGGCGGCCAAGAAGAACTTAAGCGAGGCCCTGGGG
                  ************************************************************

HsCD00329768      GACAACGTGAAACAATACTGGGCTAACCTAAAGCTGTGGTTCAAGCAGAAGATCAGCAAA
NM_053053.3       GACAACGTGAAACAATACTGGGCTAACCTAAAGCTGTGGTTCAAGCAGAAGATCAGCAAA
                  ************************************************************

HsCD00329768      GAGGAGTTTGACCTTGAAGCTCATAGACTTCTCACACAGGATAATGTCCATTCTCACAAT
NM_053053.3       GAGGAGTTTGACCTTGAAGCTCATAGACTTCTCACACAGGATAATGTCCATTCTCACAAT
                  ************************************************************

HsCD00329768      GATTTCCTCCTGGCCATTCTCACGCGTTGTCAGATTTTGGTTTCTACACCAGATGGTGCT
NM_053053.3       GATTTCCTCCTGGCCATTCTCACGCGTTGTCAGATTTTGGTTTCTACACCAGATGGTGCT
                  ************************************************************

HsCD00329768      GGATCTTTGCCTTGGCCAGGGGGTTCCGCAGCAAAACCTGGAAAACCCAAGGGAAAGAAA
NM_053053.3       GGATCTTTGCCTTGGCCAGGGGGTTCCGCAGCAAAACCTGGAAAACCCAAGGGAAAGAAA
                  ************************************************************

HsCD00329768      AAGCTTTCTTCTGTTCGTCAGAAATTTGATCATAGATTCCAGCCTCAAAATCCTCTCTCA
NM_053053.3       AAGCTTTCTTCTGTTCGTCAGAAATTTGATCATAGATTCCAGCCTCAAAATCCTCTCTCA
                  ************************************************************

HsCD00329768      GGAGCCCAGCAATTTGTGGCAAAGGATCCCCAAGATGATGACGACTTGAAACTTTGTTCC
NM_053053.3       GGAGCCCAGCAATTTGTGGCAAAGGATCCCCAAGATGATGACGACTTGAAACTTTGTTCC
                  ************************************************************

HsCD00329768      CACACAATGATGCTTCCCACTCGAGGCCAGCTTGAAGGGAGAATGATAGTGACTGCTTAT
NM_053053.3       CACACAATGATGCTTCCCACTCGAGGCCAGCTTGAAGGGAGAATGATAGTGACTGCTTAT
                  ************************************************************

HsCD00329768      GAGCATGGGCTGGACAATGTCACCGAGGAGGCTGTTTCAGCTGTTGTCTATGCTGTGGAG
NM_053053.3       GAGCATGGGCTGGACAATGTCACCGAGGAGGCTGTTTCAGCTGTTGTCTATGCTGTGGAG
                  ************************************************************

HsCD00329768      AATCACCTTAAAGATATACTGACGTCAGTTGTGTCAAGAAGGAAAGCTTATCGGTTACGA
NM_053053.3       AATCACCTTAAAGATATACTGACGTCAGTTGTGTCAAGAAGGAAAGCTTATCGGTTACGA
                  ************************************************************

HsCD00329768      GATGGTCATTTTAAATATGCCTTTGGCAGTAACGTGACCCCGCAGCCATACCTGAAGAAT
NM_053053.3       GATGGTCATTTTAAATATGCCTTTGGCAGTAACGTGACCCCGCAGCCATACCTGAAGAAT
                  ************************************************************

HsCD00329768      AGTGTAGTAGCTTACAACAACTTAATAGAAAGCCCTCCAGCTTTTACTGCTCCCTGTGCT
NM_053053.3       AGTGTAGTAGCTTACAACAACTTAATAGAAAGCCCTCCAGCTTTTACTGCTCCCTGTGCT
                  ************************************************************

HsCD00329768      GGTCAGAATCCAGCTTCTCACCCACCCCCTGATGATGCTGAGCAGCAGGCTGCACTCCTG
NM_053053.3       GGTCAGAATCCAGCTTCTCACCCACCCCCTGATGATGCTGAGCAGCAGGCTGCACTCCTG
                  ************************************************************

HsCD00329768      CTGGCATGCTCCGGAGACACTCTACCTGCATCTTTGCCTCCGGTGAACATGTACGATCTT
NM_053053.3       CTGGCATGCTCCGGAGACACTCTACCTGCATCTTTGCCTCCGGTGAACATGTACGATCTT
                  ************************************************************

HsCD00329768      TTTGAAGCTTTGCAGGTGCACAGGGAAGTCATCCCTACACATACTGTCTATGCTCTTAAC
NM_053053.3       TTTGAAGCTTTGCAGGTGCACAGGGAAGTCATCCCTACACATACTGTCTATGCTCTTAAC
                  ************************************************************

HsCD00329768      ATTGAAAGGATCATCACGAAACTCTGGCATCCAAATCATGAAGAGCTGCAGCAAGACAAA
NM_053053.3       ATTGAAAGGATCATCACGAAACTCTGGCATCCAAATCATGAAGAGCTGCAGCAAGACAAA
                  ************************************************************

HsCD00329768      GTTCACCGCCAGCGCTTGGCAGCCAAGGAGGGGCTTTTGCTGTGCTTG
NM_053053.3       GTTCACCGCCAGCGCTTGGCAGCCAAGGAGGGGCTTTTGCTGTGCTAA
                  **********************************************:.

Pathways

Reactome : Chromatin modifying enzymes
Reactome : Chromatin organization
Reactome : HATs acetylate histones

Protein

UniProt : Q96BN2
HPRD : 18114

Clone

Cloning Information : 268509
HIP Master Clone ID : 193582
Original Clone ID : FLH268509.01L

Organism


TAX_ID : 9606
Species Specific ID: 117143

Genome

Chromosome : 1
Map Location : 1q24.1
Ensembl : ENSG00000152382

Reagents

Labome : TADA1-antibody