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RNF13 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  RNF13
Gene Name:  ring finger protein 13
Sequence             
Original Clone ID: FLH268512.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors:
HsCD00329771

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1146nts         Open reading frame : 1 to 1146
Reference Sequence Alignment

ATGCTGCTCTCCATAGGGATGCTCATGCTGTCAGCCACACAAGTCTACACCATCCTGACT
GTCCAGCTCTTTGCATTCTTAAACCTACTGCCTGTAGAAGCAGACATTTTAGCATATAAC
TTTGAAAATGCATCTCAGACATTTGATGACCTCCCTGCAAGATTTGGTTATAGACTTCCA
GCTGAAGGTTTAAAGGGTTTTTTGATTAACTCAAAACCAGAGAATGCCTGTGAACCCATA
GTGCCTCCACCAGTAAAAGACAATTCATCTGGCACTTTCATCGTGTTAATTAGAAGACTT
GATTGTAATTTTGATATAAAGGTTTTAAATGCACAGAGAGCAGGATACAAGGCAGCCATA
GTTCACAATGTTGATTCTGATGACCTCATTAGCATGGGATCCAACGACATTGAGGTACTA
AAGAAAATTGACATTCCATCTGTCTTTATTGGTGAATCATCAGCTAATTCTCTGAAAGAT
GAATTCACATATGAAAAAGGGGGCCACCTTATCTTAGTTCCAGAATTTAGTCTTCCTTTG
GAATACTACCTAATTCCCTTCCTTATCATAGTGGGCATCTGTCTCATCTTGATAGTCATT
TTCATGATCACAAAATTTGTCCAGGATAGACATAGAGCTAGAAGAAACAGACTTCGTAAA
GATCAACTTAAGAAACTTCCTGTACATAAATTCAAGAAAGGAGATGAGTATGATGTATGT
GCCATTTGTTTGGATGAGTATGAAGATGGAGACAAACTCAGAATCCTTCCCTGTTCCCAT
GCTTATCATTGCAAGTGTGTAGACCCTTGGCTAACTAAAACCAAAAAAACCTGTCCAGTG
TGCAAGCAAAAAGTTGTTCCTTCTCAAGGCGATTCAGACTCTGACACAGACAGTAGTCAA
GAAGAAAATGAAGTGACAGAACATACCCCTTTACTGAGACCTTTAGCTTCTGTCAGTGCC
CAGTCATTTGGGGCTTTATCGGAATCCCGCTCACATCAGAACATGACAGAATCTTCAGAC
TATGAGGAAGACGACAATGAAGATACTGACAGTAGTGATGCAGAAAATGAAATTAATGAA
CATGATGTCGTGGTCCAGTTGCAGCCTAATGGTGAACGGGATTACAACATAGCAAATACT
GTTTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1408
Start on reference sequence 263
Mutations:
None
Discrepancy:
t55c
                                

5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : RNF13
Symbol Nomenclature : RNF13
SYNONYM : RZF
Designation : E3 ubiquitin-protein ligase RNF13|RING zinc finger protein
Full Nomenclature : ring finger protein 13
GENEID : 11342
HGNC : 10057
MIM : 609247
Vega : OTTHUMG00000150338
Target GenBank: BC009803

 

Reference Sequence Alignment

  

HsCD00329771      ATGCTGCTCTCCATAGGGATGCTCATGCTGTCAGCCACACAAGTCTACACCATCCTGACT
NM_007282.4       ATGCTGCTCTCCATAGGGATGCTCATGCTGTCAGCCACACAAGTCTACACCATCTTGACT
NM_183381.2       ATGCTGCTCTCCATAGGGATGCTCATGCTGTCAGCCACACAAGTCTACACCATCTTGACT
                  ****************************************************** *****

HsCD00329771      GTCCAGCTCTTTGCATTCTTAAACCTACTGCCTGTAGAAGCAGACATTTTAGCATATAAC
NM_007282.4       GTCCAGCTCTTTGCATTCTTAAACCTACTGCCTGTAGAAGCAGACATTTTAGCATATAAC
NM_183381.2       GTCCAGCTCTTTGCATTCTTAAACCTACTGCCTGTAGAAGCAGACATTTTAGCATATAAC
                  ************************************************************

HsCD00329771      TTTGAAAATGCATCTCAGACATTTGATGACCTCCCTGCAAGATTTGGTTATAGACTTCCA
NM_007282.4       TTTGAAAATGCATCTCAGACATTTGATGACCTCCCTGCAAGATTTGGTTATAGACTTCCA
NM_183381.2       TTTGAAAATGCATCTCAGACATTTGATGACCTCCCTGCAAGATTTGGTTATAGACTTCCA
                  ************************************************************

HsCD00329771      GCTGAAGGTTTAAAGGGTTTTTTGATTAACTCAAAACCAGAGAATGCCTGTGAACCCATA
NM_007282.4       GCTGAAGGTTTAAAGGGTTTTTTGATTAACTCAAAACCAGAGAATGCCTGTGAACCCATA
NM_183381.2       GCTGAAGGTTTAAAGGGTTTTTTGATTAACTCAAAACCAGAGAATGCCTGTGAACCCATA
                  ************************************************************

HsCD00329771      GTGCCTCCACCAGTAAAAGACAATTCATCTGGCACTTTCATCGTGTTAATTAGAAGACTT
NM_007282.4       GTGCCTCCACCAGTAAAAGACAATTCATCTGGCACTTTCATCGTGTTAATTAGAAGACTT
NM_183381.2       GTGCCTCCACCAGTAAAAGACAATTCATCTGGCACTTTCATCGTGTTAATTAGAAGACTT
                  ************************************************************

HsCD00329771      GATTGTAATTTTGATATAAAGGTTTTAAATGCACAGAGAGCAGGATACAAGGCAGCCATA
NM_007282.4       GATTGTAATTTTGATATAAAGGTTTTAAATGCACAGAGAGCAGGATACAAGGCAGCCATA
NM_183381.2       GATTGTAATTTTGATATAAAGGTTTTAAATGCACAGAGAGCAGGATACAAGGCAGCCATA
                  ************************************************************

HsCD00329771      GTTCACAATGTTGATTCTGATGACCTCATTAGCATGGGATCCAACGACATTGAGGTACTA
NM_007282.4       GTTCACAATGTTGATTCTGATGACCTCATTAGCATGGGATCCAACGACATTGAGGTACTA
NM_183381.2       GTTCACAATGTTGATTCTGATGACCTCATTAGCATGGGATCCAACGACATTGAGGTACTA
                  ************************************************************

HsCD00329771      AAGAAAATTGACATTCCATCTGTCTTTATTGGTGAATCATCAGCTAATTCTCTGAAAGAT
NM_007282.4       AAGAAAATTGACATTCCATCTGTCTTTATTGGTGAATCATCAGCTAATTCTCTGAAAGAT
NM_183381.2       AAGAAAATTGACATTCCATCTGTCTTTATTGGTGAATCATCAGCTAATTCTCTGAAAGAT
                  ************************************************************

HsCD00329771      GAATTCACATATGAAAAAGGGGGCCACCTTATCTTAGTTCCAGAATTTAGTCTTCCTTTG
NM_007282.4       GAATTCACATATGAAAAAGGGGGCCACCTTATCTTAGTTCCAGAATTTAGTCTTCCTTTG
NM_183381.2       GAATTCACATATGAAAAAGGGGGCCACCTTATCTTAGTTCCAGAATTTAGTCTTCCTTTG
                  ************************************************************

HsCD00329771      GAATACTACCTAATTCCCTTCCTTATCATAGTGGGCATCTGTCTCATCTTGATAGTCATT
NM_007282.4       GAATACTACCTAATTCCCTTCCTTATCATAGTGGGCATCTGTCTCATCTTGATAGTCATT
NM_183381.2       GAATACTACCTAATTCCCTTCCTTATCATAGTGGGCATCTGTCTCATCTTGATAGTCATT
                  ************************************************************

HsCD00329771      TTCATGATCACAAAATTTGTCCAGGATAGACATAGAGCTAGAAGAAACAGACTTCGTAAA
NM_007282.4       TTCATGATCACAAAATTTGTCCAGGATAGACATAGAGCTAGAAGAAACAGACTTCGTAAA
NM_183381.2       TTCATGATCACAAAATTTGTCCAGGATAGACATAGAGCTAGAAGAAACAGACTTCGTAAA
                  ************************************************************

HsCD00329771      GATCAACTTAAGAAACTTCCTGTACATAAATTCAAGAAAGGAGATGAGTATGATGTATGT
NM_007282.4       GATCAACTTAAGAAACTTCCTGTACATAAATTCAAGAAAGGAGATGAGTATGATGTATGT
NM_183381.2       GATCAACTTAAGAAACTTCCTGTACATAAATTCAAGAAAGGAGATGAGTATGATGTATGT
                  ************************************************************

HsCD00329771      GCCATTTGTTTGGATGAGTATGAAGATGGAGACAAACTCAGAATCCTTCCCTGTTCCCAT
NM_007282.4       GCCATTTGTTTGGATGAGTATGAAGATGGAGACAAACTCAGAATCCTTCCCTGTTCCCAT
NM_183381.2       GCCATTTGTTTGGATGAGTATGAAGATGGAGACAAACTCAGAATCCTTCCCTGTTCCCAT
                  ************************************************************

HsCD00329771      GCTTATCATTGCAAGTGTGTAGACCCTTGGCTAACTAAAACCAAAAAAACCTGTCCAGTG
NM_007282.4       GCTTATCACTGCAAGTGTGTAGACCCTTGGCTAACTAAAACCAAAAAAACCTGTCCAGTG
NM_183381.2       GCTTATCACTGCAAGTGTGTAGACCCTTGGCTAACTAAAACCAAAAAAACCTGTCCAGTG
                  ******** ***************************************************

HsCD00329771      TGCAAGCAAAAAGTTGTTCCTTCTCAAGGCGATTCAGACTCTGACACAGACAGTAGTCAA
NM_007282.4       TGCAAGCAAAAAGTTGTTCCTTCTCAAGGCGATTCAGACTCTGACACAGACAGTAGTCAA
NM_183381.2       TGCAAGCAAAAAGTTGTTCCTTCTCAAGGCGATTCAGACTCTGACACAGACAGTAGTCAA
                  ************************************************************

HsCD00329771      GAAGAAAATGAAGTGACAGAACATACCCCTTTACTGAGACCTTTAGCTTCTGTCAGTGCC
NM_007282.4       GAAGAAAATGAAGTGACAGAACATACCCCTTTACTGAGACCTTTAGCTTCTGTCAGTGCC
NM_183381.2       GAAGAAAATGAAGTGACAGAACATACCCCTTTACTGAGACCTTTAGCTTCTGTCAGTGCC
                  ************************************************************

HsCD00329771      CAGTCATTTGGGGCTTTATCGGAATCCCGCTCACATCAGAACATGACAGAATCTTCAGAC
NM_007282.4       CAGTCATTTGGGGCTTTATCGGAATCCCGCTCACATCAGAACATGACAGAATCTTCAGAC
NM_183381.2       CAGTCATTTGGGGCTTTATCGGAATCCCGCTCACATCAGAACATGACAGAATCTTCAGAC
                  ************************************************************

HsCD00329771      TATGAGGAAGACGACAATGAAGATACTGACAGTAGTGATGCAGAAAATGAAATTAATGAA
NM_007282.4       TATGAGGAAGACGACAATGAAGATACTGACAGTAGTGATGCAGAAAATGAAATTAATGAA
NM_183381.2       TATGAGGAAGACGACAATGAAGATACTGACAGTAGTGATGCAGAAAATGAAATTAATGAA
                  ************************************************************

HsCD00329771      CATGATGTCGTGGTCCAGTTGCAGCCTAATGGTGAACGGGATTACAACATAGCAAATACT
NM_007282.4       CATGATGTCGTGGTCCAGTTGCAGCCTAATGGTGAACGGGATTACAACATAGCAAATACT
NM_183381.2       CATGATGTCGTGGTCCAGTTGCAGCCTAATGGTGAACGGGATTACAACATAGCAAATACT
                  ************************************************************

HsCD00329771      GTTTTG
NM_007282.4       GTTTGA
NM_183381.2       GTTTGA
                  **** .

Clone

Cloning Information : 268512
HIP Master Clone ID : 193592
Original Clone ID : FLH268512.01L

Organism


TAX_ID : 9606
Species Specific ID: 11342

Genome

Chromosome : 3
Map Location : 3q25.1
Ensembl : ENSG00000082996

Reagents

Labome : RNF13-antibody