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ZNF193 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  ZSCAN9
Gene Name:  zinc finger and SCAN domain containing 9
Sequence             
Original Clone ID: FLH268516.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329775

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1185nts         Open reading frame : 1 to 1185
Reference Sequence Alignment

ATGAATACAAACTCAAAGGAGGTTTTATCCCTGGGTGTTCAAGTTCCCGAGGCATGGGAA
GAACTTCTGACAATGAAAGTGGAAGCAAAAAGTCACCTTCAATGGCAGGAATCCAGACTG
AAACGCAGTAATCCACTGGCAAGGGAAATCTTCCGAAGGCACTTTCGACAGCTGTGCTAC
CAAGAGACCCCTGGACCAAGGGAGGCTCTTACTCGACTCCAGGAACTTTGCTACCAGTGG
TTGAGGCCACATGTGAGCACAAAGGAGCAGATTTTGGATCTGCTGGTGCTGGAGCAGTTT
CTATCCATTCTGCCCAAGGAGCTCCAGGGCTGGGTGAGGGAACACTGTCCAGAGAGTGGA
GAAGAGGCTGTGATTTTGCTGGAGGATCTGGAGAGAGAGCTCGATGAACCACAACATGAG
ATGGTGGCCCACAGACACAGACAAGAAGTCCTCTGTAAAGAGATGGTGCCTCTAGCAGAG
CAGACACCACTGACCCTTCAGTCCCAGCCTAAGGAGCCACAGCTCACATGTGACTCTGCT
CAGAAGTGCCATTCTATTGGAGAGACAGATGAAGTAACCAAGACTGAGGACAGAGAGTTG
GTGCTAAGGAAAGACTGTCCTAAGATAGTGGAACCACATGGGAAAATGTTTAATGAGCAG
ACCTGGGAGGTATCACAGCAGGATCCCTCACATGGAGAAGTTGGTGAACATAAGGATAGG
ATAGAGAGGCAGTGGGGAAACCTCTTAGGAGAGGGGCAACACAAATGTGATGAATGTGGG
AAGAGCTTTACTCAGAGCTCAGGTCTCATTCGACATCAAAGAATTCATACTGGAGAAAGA
CCTTATGAATGTAATGAATGTGGGAAAGCCTTCAGTCGAAGTTCTGGTCTTTTTAATCAC
CGAGGAATCCACAATATACAGAAACGGTACCACTGCAAGGAGTGTGGGAAGGTCTTCAGT
CAGAGTGCGGGTCTTATCCAGCATCAGAGAATCCACAAAGGAGAAAAGCCGTATCAGTGC
AGCCAGTGCAGTAAGAGCTACAGTCGGCGTTCATTTCTCATTGAACATCAGAGAAGCCAC
ACAGGGGAGCGACCTCACCAGTGCATTGAATGTGGGAAAAGCTTTAATCGACACTGCAAC
CTCATTCGCCATCAGAAGATCCACACAGTGGCTGAGCTGGTCTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1334
Start on reference sequence 150
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ZSCAN9
Symbol Nomenclature : ZSCAN9
SYNONYM : PRD51; ZNF193
Designation : cell proliferation-inducing gene 12 protein|cell proliferation-inducing protein 12|zinc finger and SCAN domain-containing protein 9|zinc finger protein 193
Full Nomenclature : zinc finger and SCAN domain containing 9
GENEID : 7746
Locus Tag : PIG12
HGNC : 12984
MIM : 602246
Vega : OTTHUMG00000014515
Target GenBank: BC010374

 

Reference Sequence Alignment

  

HsCD00329775        ATGAATACAAACTCAAAGGAGGTTTTATCCCTGGGTGTTCAAGTTCCCGAGGCATGGGAA
NM_006299.4         ATGAATACAAACTCAAAGGAGGTTTTATCCCTGGGTGTTCAAGTTCCCGAGGCATGGGAA
NM_001199480.1      ATGAATACAAACTCAAAGGAGGTTTTATCCCTGGGTGTTCAAGTTCCCGAGGCATGGGAA
                    ************************************************************

HsCD00329775        GAACTTCTGACAATGAAAGTGGAAGCAAAAAGTCACCTTCAATGGCAGGAATCCAGACTG
NM_006299.4         GAACTTCTGACAATGAAAGTGGAAGCAAAAAGTCACCTTCAATGGCAGGAATCCAGACTG
NM_001199480.1      GAACTTCTGACAATGAAAGTGGAAGCAAAAAGTCACCTTCAATGGCAGGAATCCAGACTG
                    ************************************************************

HsCD00329775        AAACGCAGTAATCCACTGGCAAGGGAAATCTTCCGAAGGCACTTTCGACAGCTGTGCTAC
NM_006299.4         AAACGCAGTAATCCACTGGCAAGGGAAATCTTCCGAAGGCACTTTCGACAGCTGTGCTAC
NM_001199480.1      AAACGCAGTAATCCACTGGCAAGGGAAATCTTCCGAAGGCACTTTCGACAGCTGTGCTAC
                    ************************************************************

HsCD00329775        CAAGAGACCCCTGGACCAAGGGAGGCTCTTACTCGACTCCAGGAACTTTGCTACCAGTGG
NM_006299.4         CAAGAGACCCCTGGACCAAGGGAGGCTCTTACTCGACTCCAGGAACTTTGCTACCAGTGG
NM_001199480.1      CAAGAGACCCCTGGACCAAGGGAGGCTCTTACTCGACTCCAGGAACTTTGCTACCAGTGG
                    ************************************************************

HsCD00329775        TTGAGGCCACATGTGAGCACAAAGGAGCAGATTTTGGATCTGCTGGTGCTGGAGCAGTTT
NM_006299.4         TTGAGGCCACATGTGAGCACAAAGGAGCAGATTTTGGATCTGCTGGTGCTGGAGCAGTTT
NM_001199480.1      TTGAGGCCACATGTGAGCACAAAGGAGCAGATTTTGGATCTGCTGGTGCTGGAGCAGTTT
                    ************************************************************

HsCD00329775        CTATCCATTCTGCCCAAGGAGCTCCAGGGCTGGGTGAGGGAACACTGTCCAGAGAGTGGA
NM_006299.4         CTATCCATTCTGCCCAAGGAGCTCCAGGGCTGGGTGAGGGAACACTGTCCAGAGAGTGGA
NM_001199480.1      CTATCCATTCTGCCCAAGGAGCTCCAGGGCTGGGTGAGGGAACACTGTCCAGAGAGTGGA
                    ************************************************************

HsCD00329775        GAAGAGGCTGTGATTTTGCTGGAGGATCTGGAGAGAGAGCTCGATGAACCACAACATGAG
NM_006299.4         GAAGAGGCTGTGATTTTGCTGGAGGATCTGGAGAGAGAGCTCGATGAACCACAACATGAG
NM_001199480.1      GAAGAGGCTGTGATTTTGCTGGAGGATCTGGAGAGAGAGCTCGATGAACCACAACATGAG
                    ************************************************************

HsCD00329775        ATGGTGGCCCACAGACACAGACAAGAAGTCCTCTGTAAAGAGATGGTGCCTCTAGCAGAG
NM_006299.4         ATGGTGGCCCACAGACACAGACAAGAAGTCCTCTGTAAAGAGATGGTGCCTCTAGCAGAG
NM_001199480.1      ATGGTGGCCCACAGACACAGACAAGAAGTCCTCTGTAAAGAGATGGTGCCTCTAGCAGAG
                    ************************************************************

HsCD00329775        CAGACACCACTGACCCTTCAGTCCCAGCCTAAGGAGCCACAGCTCACATGTGACTCTGCT
NM_006299.4         CAGACACCACTGACCCTTCAGTCCCAGCCTAAGGAGCCACAGCTCACATGTGACTCTGCT
NM_001199480.1      CAGACACCACTGACCCTTCAGTCCCAGCCTAAGGAGCCACAGCTCACATGTGACTCTGCT
                    ************************************************************

HsCD00329775        CAGAAGTGCCATTCTATTGGAGAGACAGATGAAGTAACCAAGACTGAGGACAGAGAGTTG
NM_006299.4         CAGAAGTGCCATTCTATTGGAGAGACAGATGAAGTAACCAAGACTGAGGACAGAGAGTTG
NM_001199480.1      CAGAAGTGCCATTCTATTGGAGAGACAGATGAAGTAACCAAGACTGAGGACAGAGAGTTG
                    ************************************************************

HsCD00329775        GTGCTAAGGAAAGACTGTCCTAAGATAGTGGAACCACATGGGAAAATGTTTAATGAGCAG
NM_006299.4         GTGCTAAGGAAAGACTGTCCTAAGATAGTGGAACCACATGGGAAAATGTTTAATGAGCAG
NM_001199480.1      GTGCTAAGGAAAGACTGTCCTAAGATAGTGGAACCACATGGGAAAATGTTTAATGAGCAG
                    ************************************************************

HsCD00329775        ACCTGGGAGGTATCACAGCAGGATCCCTCACATGGAGAAGTTGGTGAACATAAGGATAGG
NM_006299.4         ACCTGGGAGGTATCACAGCAGGATCCCTCACATGGAGAAGTTGGTGAACATAAGGATAGG
NM_001199480.1      ACCTGGGAGGTATCACAGCAGGATCCCTCACATGGAGAAGTTGGTGAACATAAGGATAGG
                    ************************************************************

HsCD00329775        ATAGAGAGGCAGTGGGGAAACCTCTTAGGAGAGGGGCAACACAAATGTGATGAATGTGGG
NM_006299.4         ATAGAGAGGCAGTGGGGAAACCTCTTAGGAGAGGGGCAACACAAATGTGATGAATGTGGG
NM_001199480.1      ATAGAGAGGCAGTGGGGAAACCTCTTAGGAGAGGGGCAACACAAATGTGATGAATGTGGG
                    ************************************************************

HsCD00329775        AAGAGCTTTACTCAGAGCTCAGGTCTCATTCGACATCAAAGAATTCATACTGGAGAAAGA
NM_006299.4         AAGAGCTTTACTCAGAGCTCAGGTCTCATTCGACATCAAAGAATTCATACTGGAGAAAGA
NM_001199480.1      AAGAGCTTTACTCAGAGCTCAGGTCTCATTCGACATCAAAGAATTCATACTGGAGAAAGA
                    ************************************************************

HsCD00329775        CCTTATGAATGTAATGAATGTGGGAAAGCCTTCAGTCGAAGTTCTGGTCTTTTTAATCAC
NM_006299.4         CCTTATGAATGTAATGAATGTGGGAAAGCCTTCAGTCGAAGTTCTGGTCTTTTTAATCAC
NM_001199480.1      CCTTATGAATGTAATGAATGTGGGAAAGCCTTCAGTCGAAGTTCTGGTCTTTTTAATCAC
                    ************************************************************

HsCD00329775        CGAGGAATCCACAATATACAGAAACGGTACCACTGCAAGGAGTGTGGGAAGGTCTTCAGT
NM_006299.4         CGAGGAATCCACAATATACAGAAACGGTACCACTGCAAGGAGTGTGGGAAGGTCTTCAGT
NM_001199480.1      CGAGGAATCCACAATATACAGAAACGGTACCACTGCAAGGAGTGTGGGAAGGTCTTCAGT
                    ************************************************************

HsCD00329775        CAGAGTGCGGGTCTTATCCAGCATCAGAGAATCCACAAAGGAGAAAAGCCGTATCAGTGC
NM_006299.4         CAGAGTGCGGGTCTTATCCAGCATCAGAGAATCCACAAAGGAGAAAAGCCGTATCAGTGC
NM_001199480.1      CAGAGTGCGGGTCTTATCCAGCATCAGAGAATCCACAAAGGAGAAAAGCCGTATCAGTGC
                    ************************************************************

HsCD00329775        AGCCAGTGCAGTAAGAGCTACAGTCGGCGTTCATTTCTCATTGAACATCAGAGAAGCCAC
NM_006299.4         AGCCAGTGCAGTAAGAGCTACAGTCGGCGTTCATTTCTCATTGAACATCAGAGAAGCCAC
NM_001199480.1      AGCCAGTGCAGTAAGAGCTACAGTCGGCGTTCATTTCTCATTGAACATCAGAGAAGCCAC
                    ************************************************************

HsCD00329775        ACAGGGGAGCGACCTCACCAGTGCATTGAATGTGGGAAAAGCTTTAATCGACACTGCAAC
NM_006299.4         ACAGGGGAGCGACCTCACCAGTGCATTGAATGTGGGAAAAGCTTTAATCGACACTGCAAC
NM_001199480.1      ACAGGGGAGCGACCTCACCAGTGCATTGAATGTGGGAAAAGCTTTAATCGACACTGCAAC
                    ************************************************************

HsCD00329775        CTCATTCGCCATCAGAAGATCCACACAGTGGCTGAGCTGGTCTTG
NM_006299.4         CTCATTCGCCATCAGAAGATCCACACAGTGGCTGAGCTGGTCTAG
NM_001199480.1      CTCATTCGCCATCAGAAGATCCACACAGTGGCTGAGCTGGTCTAG
                    *******************************************:*

Protein

SMART domain : ZnF_C2H2 : zinc finger
SMART domain : SCAN : leucine rich region
UniProt : O15535
UniProt : E7EVQ2
UniProt : B4E1W6
HPRD : 03766

Clone

Cloning Information : 268516
HIP Master Clone ID : 193600
Original Clone ID : FLH268516.01L

Organism


TAX_ID : 9606
Species Specific ID: 7746

Genome

Chromosome : 6
Map Location : 6p21.3
Ensembl : ENSG00000137185

Reagents

Labome : ZSCAN9-antibody