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SSX2 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  SSX2B
Gene Name:  synovial sarcoma, X breakpoint 2B
Sequence             
Original Clone ID: FLH268525.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329784

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 672nts         Open reading frame : 1 to 672
Reference Sequence Alignment

ATGAACGGAGACGACGCCTTTGCAAGGAGACCCACGGTTGGTGCTCAAATACCAGAGAAG
ATCCAAAAGGCCTTCGATGATATTGCCAAATACTTCTCTAAGGAAGAGTGGGAAAAGATG
AAAGCCTCGGAGAAAATCTTCTATGTGTATATGAAGAGAAAGTATGAGGCTATGACTAAA
CTAGGTTTCAAGGCCACCCTCCCACCTTTCATGTGTAATAAACGGGCCGAAGACTTCCAG
GGGAATGATTTGGATAATGACCCTAACCGTGGGAATCAGGTTGAACGTCCTCAGATGACT
TTCGGCAGGCTCCAGGGAATCTCCCCGAAGATCATGCCCAAGAAGCCAGCAGAGGAAGGA
AATGATTCGGAGGAAGTGCCAGAAGCATCTGGCCCACAAAATGATGGGAAAGAGCTGTGC
CCCCCGGGAAAACCAACTACCTCTGAGAAGATTCACGAGAGATCTGGAAATAGGGAGGCC
CAAGAAAAGGAAGAGAGACGCGGAACAGCTCATCGGTGGAGCAGTCAGAACACACACAAC
ATTGGTCGATTCAGTTTGTCAACTTCTATGGGTGCAGTTCATGGTACCCCCAAAACAATT
ACACACAACAGGGACCCAAAAGGGGGGAACATGCCTGGACCCACAGACTGCGTGAGAGAA
AACAGCTGGTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 742
Start on reference sequence 71
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : SSX2B
Symbol Nomenclature : SSX2B
SYNONYM : CT5.2; CT5.2b; HOM-MEL-40; SSX
Designation : cancer/testis antigen 5.2|cancer/testis antigen family 5, member 2b|protein SSX2|tumor antigen HOM-MEL-40
Full Nomenclature : synovial sarcoma, X breakpoint 2B
GENEID : 727837
Locus Tag : RP13-77O11.9
HGNC : 22263
Vega : OTTHUMG00000021593
Target GenBank: BC071827

 

Reference Sequence Alignment

  

HsCD00329784        ATGAACGGAGACGACGCCTTTGCAAGGAGACCCACGGTTGGTGCTCAAATACCAGAGAAG
XM_005262001.1      ATGAACGGAGACGACGCCTTTGCAAGGAGACCTAGGGCTGGTGCTCAAATACCAGAGAAG
NM_001278701.1      ATGAACGGAGACGACGCCTTTGCAAGGAGACCCACGGTTGGTGCTCAAATACCAGAGAAG
NM_003147.5         ATGAACGGAGACGACGCCTTTGCAAGGAGACCCACGGTTGGTGCTCAAATACCAGAGAAG
                    ******************************** * ** **********************

HsCD00329784        ATCCAAAAG---------------------------------------------------
XM_005262001.1      ATCCAAAAGCATCCTTGGAGACAAGTCTGTGACCGTGCACTACATTTGGTGAATCTCAGT
NM_001278701.1      ATCCAAAAG---------------------------------------------------
NM_003147.5         ATCCAAAAG---------------------------------------------------
                    *********                                                   

HsCD00329784        ------------------------------------------------------------
XM_005262001.1      CCATTCTGGAAAGTGGGAAGAGAGTCAGTCAGCAGCATTAAAGCCCTACTGTGTGGCAGG
NM_001278701.1      ------------------------------------------------------------
NM_003147.5         ------------------------------------------------------------
                                                                                

HsCD00329784        ------------GCCTTCGATGATATTGCCAAATACTTCTCTAAGGAAGAGTGGGAAAAG
XM_005262001.1      GGTGAAGCTAGGTCCTTCGATGATATTGCCAAATACTTCTCTAAGAAAGAGTGGGAAAAG
NM_001278701.1      ------------GCCTTCGATGATATTGCCAAATACTTCTCTAAGGAAGAGTGGGAAAAG
NM_003147.5         ------------GCCTTCGATGATATTGCCAAATACTTCTCTAAGGAAGAGTGGGAAAAG
                                 ********************************.**************

HsCD00329784        ATGAAAGCCTCGGAGAAAATCTTCTATGTGTATATGAAGAGAAAGTATGAGGCTATGACT
XM_005262001.1      ATGAAATCCTTGGAGAAAATCAGCTATGTGTATATGAAGAGAAAGTATGAGGCCATGACT
NM_001278701.1      ATGAAAGCCTCGGAGAAAATCTTCTATGTGTATATGAAGAGAAAGTATGAGGCTATGACT
NM_003147.5         ATGAAAGCCTCGGAGAAAATCTTCTATGTGTATATGAAGAGAAAGTATGAGGCTATGACT
                    ****** *** **********: ****************************** ******

HsCD00329784        AAACTAGGTTTCAAGGCCACCCTCCCACCTTTCATGTGTAATAAACGGGCCGAAGACTTC
XM_005262001.1      AAACTAGGCTTCAAGGCCACCCTCCCACCTTTCATGCATAATACAGGGGCCACAGACCTC
NM_001278701.1      AAACTAGGTTTCAAGGCCACCCTCCCACCTTTCATGTGTAATAAACGGGCCGAAGACTTC
NM_003147.5         AAACTAGGTTTCAAGGCCACCCTCCCACCTTTCATGTGTAATAAACGGGCCGAAGACTTC
                    ******** *************************** .*****.* *****..**** **

HsCD00329784        CAGGGGAATGATTTGGATAATGACCCTAACCGTGGGAATCAGGTTGAACGTCCTCAGATG
XM_005262001.1      CAGGGGAATGATTTTGATAATGACCGTAACCAAGGGAATCAGGTTGAACGTCCTCAGATG
NM_001278701.1      CAGGGGAATGATTTGGATAATGACCCTAACCGTGGGAATCAGGTTGAACGTCCTCAGATG
NM_003147.5         CAGGGGAATGATTTGGATAATGACCCTAACCGTGGGAATCAGGTTGAACGTCCTCAGATG
                    ************** ********** *****.:***************************

HsCD00329784        ACTTTCGGCAGGCTCCAGGGAATCTCCCCGAAGATCATGCCCAAGAAGCCAGCAGAGGAA
XM_005262001.1      ACTTTTTGCAGGCTCCAGAGAATCTTCCCGAAGATCATGCCCAAGAAGCCAGCAGAGGAA
NM_001278701.1      ACTTTCGGCAGGCTCCAGGGAATCTCCCCGAAGATCATGCCCAAGAAGCCAGCAGAGGAA
NM_003147.5         ACTTTCGGCAGGCTCCAGGGAATCTCCCCGAAGATCATGCCCAAGAAGCCAGCAGAGGAA
                    *****  ***********.****** **********************************

HsCD00329784        GGAAATGATTCGGAGGAAGTGCCAGAAGCATCTGGCCCACAAAATGATGGGAAAGAGCTG
XM_005262001.1      GGAAATGATTCGAAGGGAGTGCCAGAAGCATCTGGCTCACAGAACGATGGGAAACACCTG
NM_001278701.1      GGAAATGATTCGGAGGAAGTGCCAGAAGCATCTGGCCCACAAAATGATGGGAAAGAGCTG
NM_003147.5         GGAAATGATTCGGAGGAAGTGCCAGAAGCATCTGGCCCACAAAATGATGGGAAAGAGCTG
                    ************.***.******************* ****.** ********* * ***

HsCD00329784        TGCCCCCCGGGAAAACCAACTACCTCTGAGAAGATTCACGAGAGATCTGGAAATAGGGAG
XM_005262001.1      TGCCCTCCAGGAAAACCAAGTACCTCTGAGAAGATTAACAAGACATCCG----------G
NM_001278701.1      TGCCCCCCGGGAAAACCAACTACCTCTGAGAAGATTCACGAGAGATCTGGAAATAGGGAG
NM_003147.5         TGCCCCCCGGGAAAACCAACTACCTCTGAGAAGATTCACGAGAGATCTGGAAATAGGGAG
                    ***** **.********** ****************.**.*** *** *          *

HsCD00329784        GCCCAAGAAAAGGAAGAGAGACGCGGAACAGCTCATCGGTGGAGCAGTCAGAACACACAC
XM_005262001.1      ACCCAAAAGGGGGAAACATGCCTGG-------------------------ACCCACAGAC
NM_001278701.1      GCCCAAGAAAAGGAAGAGAGACGCGGAACAGCTCATCGGTGGAGCAGTCAGAACACACAC
NM_003147.5         GCCCAAGAAAAGGAAGAGAGACGCGGAACAGCTCATCGGTGGAGCAGTCAGAACACACAC
                    .*****.*...****...:*.*  *                         ...**** **

HsCD00329784        AACATTGGTCGATTCAGTTTGTCAACTTCTATGGGTGCAGTTCATGGTACCCCCAAAACA
XM_005262001.1      TGCGTGAGAGAAAGCAGCTGGTGATTTATGAAG---------------------------
NM_001278701.1      AACATTGGTCGATTCAGTTTGTCAACTTCTATGGGTGCAGTTCATGGTACCCCCAAAACA
NM_003147.5         AACATTGGTCGATTCAGTTTGTCAACTTCTATGGGTGCAGTTCATGGTACCCCCAAAACA
                    :.*.* .*: .*: *** * ** *: *:  *:*                           

HsCD00329784        ATTACACACAACAGGGACCCAAAAGGGGGGAACATGCCTGGACCCACAGACTGCGTGAGA
XM_005262001.1      -------AGATCAGCGACCCTGAAGAAGACGACGAGTAA---------------------
NM_001278701.1      ATTACACACAACAGGGACCCAAAAGGGGGGAACATGCCTGGACCCACAGACTGCGTGAGA
NM_003147.5         ATTACACACAACAGGGACCCAAAAGGGGGGAACATGCCTGGACCCACAGACTGCGTGAGA
                           * *:*** *****:.***..*. .**.:* .:                     

HsCD00329784        GAAAACAGCTGGTTG
XM_005262001.1      ---------------
NM_001278701.1      GAAAACAGCTGGTGA
NM_003147.5         GAAAACAGCTGGTGA
                                   

Protein

SMART domain : KRAB : krueppel associated box
UniProt : Q16385

Clone

Cloning Information : 268525
HIP Master Clone ID : 194768
Original Clone ID : FLH268525.01L

Organism


TAX_ID : 9606
Species Specific ID: 6757

Genome

Chromosome : X
Map Location : Xp11.22
Ensembl : ENSG00000157950

Reagents

Labome : SSX2-antibody