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TRIM35 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  TRIM35
Gene Name:  tripartite motif containing 35
Sequence             
Original Clone ID: FLH268528.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329787

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1482nts         Open reading frame : 1 to 1482
Reference Sequence Alignment

ATGGAGCGGAGTCCCGACGTGTCCCCCGGGCCTTCCCGCTCCTTCAAGGAGGAGTTGCTC
TGCGCCGTCTGCTACGACCCCTTCCGCGACGCAGTCACTCTGCGCTGCGGCCACAACTTC
TGCCGCGGGTGCGTGAGCCGCTGCTGGGAGGTGCAGGTGTCGCCCACCTGCCCAGTGTGC
AAAGACCGCGCGTCACCCGCCGACCTGCGCACCAACCACACCCTCAACAACCTGGTGGAG
AAGCTGCTGCGCGAGGAGGCCGAGGGCGCGCGCTGGACCAGCTACCGCTTCTCGCGTGTC
TGCCGCCTGCACCGCGGACAGCTCAGCCTCTTCTGCCTCGAGGACAAGGAGCTGCTGTGC
TGCTCCTGCCAGGCCGACCCCCGACACCAGGGGCACCGCGTGCAGCCGGTGAAGGACACT
GCCCACGACTTTCGGGCCAAGTGCAGGAACATGGAGCATGCACTGCGGGAGAAGGCCAAG
GCCTTCTGGGCCATGCGGCGCTCCTATGAGGCCATCGCCAAGCACAATCAGGTGGAGGCT
GCATGGCTGGAAGGCCGGATCCGGCAGGAGTTTGATAAGCTTCGCGAGTTCTTGAGAGTG
GAGGAGCAGGCCATTCTGGATGCCATGGCCGAGGAGACAAGGCAGAAGCAACTTCTGGCC
GACGAGAAGATGAAGCAGCTCACAGAGGAGACGGAGGTGCTGGCACATGAGATCGAGCGG
CTGCAGATGGAGATGAAGGAGGACGACGTTTCTTTTCTCATGAAACACAAGAGCCGAAAA
CGCCGACTCTTCTGCACCATGGAGCCAGAGCCAGTCCAGCCCGGCATGCTTATCGATGTC
TGCAAGTACCTGGGCTCCCTGCAGTACCGCGTCTGGAAGAAGATGCTTGCATCTGTGGAA
TCTGTACCCTTCAGCTTTGACCCCAACACCGCAGCTGGCTGGCTCTCCGTGTCTGACGAC
CTCACCAGCGTCACCAACCATGGCTACCGCGTGCAGGTGGAGAACCCGGAACGCTTCTCC
TCGGCGCCCTGCCTGCTGGGCTCCCGTGTCTTCTCACAGGGCTCGCACGCCTGGGAGGTG
GCCCTTGGGGGGCTGCAGAGCTGGAGGGTGGGCGTGGTACGTGTGCGCCAGGACTCGGGC
GCTGAGGGCCACTCACACAGCTGCTACCACGACACACGCTCGGGCTTCTGGTATGTCTGC
CGCACGCAGGGCGTGGAGGGGGACCACTGCGTGACCTCGGACCCAGCCACGTCGCCCCTG
GTCCTGGCCATCCCACGCCGCCTGCGTGTGGAGCTGGAGTGTGAGGAGGGCGAGCTGTCT
TTCTATGACGCGGAGCGCCACTGCCACCTGTACACCTTCCACGCCCGCTTTGGGGAGGTT
CGCCCCTACTTCTACCTGGGGGGTGCACGGGGCGCCGGGCCTCCAGAGCCTTTGCGCATC
TGCCCCTTGCACATCAGTGTCAAGGAAGAACTGGATGGCTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1508
Start on reference sequence 27
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : TRIM35
Symbol Nomenclature : TRIM35
SYNONYM : HLS5; MAIR
Designation : hemopoietic lineage switch protein 5|tripartite motif-containing 35|tripartite motif-containing protein 35
Full Nomenclature : tripartite motif containing 35
GENEID : 23087
HGNC : 16285
Vega : OTTHUMG00000102047
Target GenBank: BC069226

 

Reference Sequence Alignment

  

HsCD00329787        ATGGAGCGGAGTCCCGACGTGTCCCCCGGGCCTTCCCGCTCCTTCAAGGAGGAGTTGCTC
NM_171982.3         ATGGAGCGGAGTCCCGACGTGTCCCCCGGGCCTTCCCGCTCCTTCAAGGAGGAGTTGCTC
XM_005273451.1      ATGGAGCGGAGTCCCGACGTGTCCCCCGGGCCTTCCCGCTCCTTCAAGGAGGAGTTGCTC
                    ************************************************************

HsCD00329787        TGCGCCGTCTGCTACGACCCCTTCCGCGACGCAGTCACTCTGCGCTGCGGCCACAACTTC
NM_171982.3         TGCGCCGTCTGCTACGACCCCTTCCGCGACGCAGTCACTCTGCGCTGCGGCCACAACTTC
XM_005273451.1      TGCGCCGTCTGCTACGACCCCTTCCGCGACGCAGTCACTCTGCGCTGCGGCCACAACTTC
                    ************************************************************

HsCD00329787        TGCCGCGGGTGCGTGAGCCGCTGCTGGGAGGTGCAGGTGTCGCCCACCTGCCCAGTGTGC
NM_171982.3         TGCCGCGGGTGCGTGAGCCGCTGCTGGGAGGTGCAGGTGTCGCCCACCTGCCCAGTGTGC
XM_005273451.1      TGCCGCGGGTGCGTGAGCCGCTGCTGGGAGGTGCAGGTGTCGCCCACCTGCCCAGTGTGC
                    ************************************************************

HsCD00329787        AAAGACCGCGCGTCACCCGCCGACCTGCGCACCAACCACACCCTCAACAACCTGGTGGAG
NM_171982.3         AAAGACCGCGCGTCACCCGCCGACCTGCGCACCAACCACACCCTCAACAACCTGGTGGAG
XM_005273451.1      AAAGACCGCGCGTCACCCGCCGACCTGCGCACCAACCACACCCTCAACAACCTGGTGGAG
                    ************************************************************

HsCD00329787        AAGCTGCTGCGCGAGGAGGCCGAGGGCGCGCGCTGGACCAGCTACCGCTTCTCGCGTGTC
NM_171982.3         AAGCTGCTGCGCGAGGAGGCCGAGGGCGCGCGCTGGACCAGCTACCGCTTCTCGCGTGTC
XM_005273451.1      AAGCTGCTGCGCGAGGAGGCCGAGGGCGCGCGCTGGACCAGCTACCGCTTCTCGCGTGTC
                    ************************************************************

HsCD00329787        TGCCGCCTGCACCGCGGACAGCTCAGCCTCTTCTGCCTCGAGGACAAGGAGCTGCTGTGC
NM_171982.3         TGCCGCCTGCACCGCGGACAGCTCAGCCTCTTCTGCCTCGAGGACAAGGAGCTGCTGTGC
XM_005273451.1      TGCCGCCTGCACCGCGGACAGCTCAGCCTCTTCTGCCTCGAGGACAAGGAGCTGCTGTGC
                    ************************************************************

HsCD00329787        TGCTCCTGCCAGGCCGACCCCCGACACCAGGGGCACCGCGTGCAGCCGGTGAAGGACACT
NM_171982.3         TGCTCCTGCCAGGCCGACCCCCGACACCAGGGGCACCGCGTGCAGCCGGTGAAGGACACT
XM_005273451.1      TGCTCCTGCCAGGCCGACCCCCGACACCAGGGGCACCGCGTGCAGCCGGTGAAGGACACT
                    ************************************************************

HsCD00329787        GCCCACGACTTTCGGGCCAAGTGCAGGAACATGGAGCATGCACTGCGGGAGAAGGCCAAG
NM_171982.3         GCCCACGACTTTCGGGCCAAGTGCAGGAACATGGAGCATGCACTGCGGGAGAAGGCCAAG
XM_005273451.1      GCCCACGACTTTCGG---------------------------------------------
                    ***************                                             

HsCD00329787        GCCTTCTGGGCCATGCGGCGCTCCTATGAGGCCATCGCCAAGCACAATCAGGTGGAGGCT
NM_171982.3         GCCTTCTGGGCCATGCGGCGCTCCTATGAGGCCATCGCCAAGCACAATCAGGTGGAGGCT
XM_005273451.1      ---------------------------------------------------GTGGAGGCT
                                                                       *********

HsCD00329787        GCATGGCTGGAAGGCCGGATCCGGCAGGAGTTTGATAAGCTTCGCGAGTTCTTGAGAGTG
NM_171982.3         GCATGGCTGGAAGGCCGGATCCGGCAGGAGTTTGATAAGCTTCGCGAGTTCTTGAGAGTG
XM_005273451.1      GCATGGCTGGAAGGCCGGATCCGGCAGGAGTTTGATAAGCTTCGCGAGTTCTTGAGAGTG
                    ************************************************************

HsCD00329787        GAGGAGCAGGCCATTCTGGATGCCATGGCCGAGGAGACAAGGCAGAAGCAACTTCTGGCC
NM_171982.3         GAGGAGCAGGCCATTCTGGATGCCATGGCCGAGGAGACAAGGCAGAAGCAACTTCTGGCC
XM_005273451.1      GAGGAGCAGGCCATTCTGGATGCCATGGCCGAGGAGACAAGGCAGAAGCAACTTCTGGCC
                    ************************************************************

HsCD00329787        GACGAGAAGATGAAGCAGCTCACAGAGGAGACGGAGGTGCTGGCACATGAGATCGAGCGG
NM_171982.3         GACGAGAAGATGAAGCAGCTCACAGAGGAGACGGAGGTGCTGGCACATGAGATCGAGCGG
XM_005273451.1      GACGAGAAGATGAAGCAGCTCACAGAGGAGACGGAGGTGCTGGCACATGAGATCGAGCGG
                    ************************************************************

HsCD00329787        CTGCAGATGGAGATGAAGGAGGACGACGTTTCTTTTCTCATGAAACACAAGAGCCGAAAA
NM_171982.3         CTGCAGATGGAGATGAAGGAGGACGACGTTTCTTTTCTCATGAAACACAAGAGCCGAAAA
XM_005273451.1      CTGCAGATGGAGATGAAGGAGGACGACGTTTCTTTTCTCATGAAACACAAGAGCCGAAAA
                    ************************************************************

HsCD00329787        CGCCGACTCTTCTGCACCATGGAGCCAGAGCCAGTCCAGCCCGGCATGCTTATCGATGTC
NM_171982.3         CGCCGACTCTTCTGCACCATGGAGCCAGAGCCAGTCCAGCCCGGCATGCTTATCGATGTC
XM_005273451.1      CGCCGACTCTTCTGCACCATGGAGCCAGAGCCAGTCCAGCCCGGCATGCTTATCGATGTC
                    ************************************************************

HsCD00329787        TGCAAGTACCTGGGCTCCCTGCAGTACCGCGTCTGGAAGAAGATGCTTGCATCTGTGGAA
NM_171982.3         TGCAAGTACCTGGGCTCCCTGCAGTACCGCGTCTGGAAGAAGATGCTTGCATCTGTGGAA
XM_005273451.1      TGCAAGTACCTGGGCTCCCTGCAGTACCGCGTCTGGAAGAAGATGCTTGCATCTGTGGAA
                    ************************************************************

HsCD00329787        TCTGTACCCTTCAGCTTTGACCCCAACACCGCAGCTGGCTGGCTCTCCGTGTCTGACGAC
NM_171982.3         TCTGTACCCTTCAGCTTTGACCCCAACACCGCAGCTGGCTGGCTCTCCGTGTCTGACGAC
XM_005273451.1      TCTGTACCCTTCAGCTTTGACCCCAACACCGCAGCTGGCTGGCTCTCCGTGTCTGACGAC
                    ************************************************************

HsCD00329787        CTCACCAGCGTCACCAACCATGGCTACCGCGTGCAGGTGGAGAACCCGGAACGCTTCTCC
NM_171982.3         CTCACCAGCGTCACCAACCATGGCTACCGCGTGCAGGTGGAGAACCCGGAACGCTTCTCC
XM_005273451.1      CTCACCAGCGTCACCAACCATGGCTACCGCGTGCAGGTGGAGAACCCGGAACGCTTCTCC
                    ************************************************************

HsCD00329787        TCGGCGCCCTGCCTGCTGGGCTCCCGTGTCTTCTCACAGGGCTCGCACGCCTGGGAGGTG
NM_171982.3         TCGGCGCCCTGCCTGCTGGGCTCCCGTGTCTTCTCACAGGGCTCGCACGCCTGGGAGGTG
XM_005273451.1      TCGGCGCCCTGCCTGCTGGGCTCCCGTGTCTTCTCACAGGGCTCGCACGCCTGGGAGGTG
                    ************************************************************

HsCD00329787        GCCCTTGGGGGGCTGCAGAGCTGGAGGGTGGGCGTGGTACGTGTGCGCCAGGACTCGGGC
NM_171982.3         GCCCTTGGGGGGCTGCAGAGCTGGAGGGTGGGCGTGGTACGTGTGCGCCAGGACTCGGGC
XM_005273451.1      GCCCTTGGGGGGCTGCAGAGCTGGAGGGTGGGCGTGGTACGTGTGCGCCAGGACTCGGGC
                    ************************************************************

HsCD00329787        GCTGAGGGCCACTCACACAGCTGCTACCACGACACACGCTCGGGCTTCTGGTATGTCTGC
NM_171982.3         GCTGAGGGCCACTCACACAGCTGCTACCACGACACACGCTCGGGCTTCTGGTATGTCTGC
XM_005273451.1      GCTGAGGGCCACTCACACAGCTGCTACCACGACACACGCTCGGGCTTCTGGTATGTCTGC
                    ************************************************************

HsCD00329787        CGCACGCAGGGCGTGGAGGGGGACCACTGCGTGACCTCGGACCCAGCCACGTCGCCCCTG
NM_171982.3         CGCACGCAGGGCGTGGAGGGGGACCACTGCGTGACCTCGGACCCAGCCACGTCGCCCCTG
XM_005273451.1      CGCACGCAGGGCGTGGAGGGGGACCACTGCGTGACCTCGGACCCAGCCACGTCGCCCCTG
                    ************************************************************

HsCD00329787        GTCCTGGCCATCCCACGCCGCCTGCGTGTGGAGCTGGAGTGTGAGGAGGGCGAGCTGTCT
NM_171982.3         GTCCTGGCCATCCCACGCCGCCTGCGTGTGGAGCTGGAGTGTGAGGAGGGCGAGCTGTCT
XM_005273451.1      GTCCTGGCCATCCCACGCCGCCTGCGTGTGGAGCTGGAGTGTGAGGAGGGCGAGCTGTCT
                    ************************************************************

HsCD00329787        TTCTATGACGCGGAGCGCCACTGCCACCTGTACACCTTCCACGCCCGCTTTGGGGAGGTT
NM_171982.3         TTCTATGACGCGGAGCGCCACTGCCACCTGTACACCTTCCACGCCCGCTTTGGGGAGGTT
XM_005273451.1      TTCTATGACGCGGAGCGCCACTGCCACCTGTACACCTTCCACGCCCGCTTTGGGGAGGTT
                    ************************************************************

HsCD00329787        CGCCCCTACTTCTACCTGGGGGGTGCACGGGGCGCCGGGCCTCCAGAGCCTTTGCGCATC
NM_171982.3         CGCCCCTACTTCTACCTGGGGGGTGCACGGGGCGCCGGGCCTCCAGAGCCTTTGCGCATC
XM_005273451.1      CGCCCCTACTTCTACCTGGGGGGTGCACGGGGCGCCGGGCCTCCAGAGCCTTTGCGCATC
                    ************************************************************

HsCD00329787        TGCCCCTTGCACATCAGTGTCAAGGAAGAACTGGATGGCTTG
NM_171982.3         TGCCCCTTGCACATCAGTGTCAAGGAAGAACTGGATGGCTGA
XM_005273451.1      TGCCCCTTGCACATCAGTGTCAAGGAAGAACTGGATGGCTGA
                    **************************************** .

Protein

SMART domain : SPRY : Domain in SPla and the RYanodine Receptor.
SMART domain : BBOX : B-Box-type zinc finger
SMART domain : PRY :
SMART domain : RING : Ring finger
UniProt : Q9UPQ4
HPRD : 15553

Clone

Cloning Information : 268528
HIP Master Clone ID : 194773
Original Clone ID : FLH268528.01L

Organism


TAX_ID : 9606
Species Specific ID: 23087

Genome

Chromosome : 8
Map Location : 8p21.2
Ensembl : ENSG00000104228

Reagents

Labome : HLS5-antibody