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MGC16733 (H. sapiens ) in pLenti6.2/V5-DEST

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Explanation of Terms


Gene: Gene Symbol:  INTS4
Gene Name:  integrator complex subunit 4
Sequence             
Original Clone ID: FLH268529.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors:
HsCD00329788

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1518nts         Open reading frame : 1 to 1518
Reference Sequence Alignment

ATGGCGGCGCACCTTAAGAAGCGGGTTTATGAGGAATTCACGAAAGTGGTTCAGCCACAG
GAGGAAATTGCTACTAAGAAACTCCGACTAACAAAACCAAGTAAATCTGCAGCACTCCAC
ATAGATCTGTGTAAAGCTACCTCCCCAGCAGATGCTTTGCAATACTTGCTCCAGTTTGCC
AGGAAGCCTGTCGAGGCGGAAAGCGTAGAGGGAGTAGTCAGGATTCTCTTGGAACATTAT
TACAAGGAGAATGATCCATCTGTGAGACTGAAAATTGCATCATTGTTGGGTTTATTATCA
AAGACAGCAGGATTTTCACCAGACTGCATTATGGATGATGCCATCAACATCCTGCAGAAT
GAAAAGTCTCATCAAGTCCTAGCTCAACTGCTGGATACTTTGCTTGCAATTGGCACTAAG
CTACCAGAGAATCAAGCTATCCAAATGCGATTAGTTGATGTGGCCTGCAAGCATCTGACA
GATACGTCTCATGGTGTAAGAAATAAGTGCCTGCAGTTACTTGGCAATCTTGGCTCTTTG
GAGAAAAGTGTCACAAAAGATGCAGAAGGCCTAGCTGCCAGAGATGTCCAGAAGATTATA
GGGGATTACTTCAGTGACCAAGACCCACGTGTCAGAACAGCAGCTATAAAAGCCATGTTG
CAGCTCCATGAAAGAGGACTGAAATTACACCAAACAATTTATAATCAGGCCTGTAAATTA
CTCTCTGATGACTATGAACAAGTGCGCAGTGCTGCAGTCCAGCTTATCTGGGTCGTCAGT
CAGCTCTATCCTGAAAGCATTGTCCCAATTCCTTCTTCTAATGAAGAAATACGCTTAGTT
GATGATGCGTTTGGCAAAATTTGTCACATGGTCAGTGATGGCTCTTGGGTGGTTCGTGTT
CAGGCAGCAAAACTGTTGGGCTCTATGGAGCAAGTCAGTTCTCATTTCTTGGAGCAGACC
CTTGACAAGAAGCTGATGTCAGATCTGAGGAGGAAACGTACTGCACATGAGCGTGCCAAG
GAACTTTACAGTTCGGGGGAGTTTTCCAGTGGCAGAAAGTGGGGAGATGATGCTCCCAAG
GAAGAAGTAGATACCGGGGCTGTGAACTTGATTGAGTCAGGAGCTTGTGGAGCTTTTGTT
CATGGGTTGGAAGATGAGATGTATGAGGTTCGTATTGCTGCTGTGGAGGCCCTCTGCATG
TTGGCCCAGTCTTCACCCTCTTATGCTGAGAAGTGCCTTGATTTCCTAGTTGACATGTTC
AACGATGAAATTGAGGAAGTACGTCTGCAGTCTATACATACCATGAGAAAAATCTCTAAC
AACATCACCCTCCGAGAAGATCAGCTTGACACTGTCCTGGCTGTGCTAGAGGATTCATCC
AGAGATATTCGAGAGGCTCTTCATGAACTCTTATGCTGTACTAATGTTTCAACCAAAGAA
GGGATTCATCTTGCATTGGTGGAGCTGCTGAAAAATTTAACCAAGTACCCTACTGATAGG
GACTCCATATGGAAGTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1550
Start on reference sequence 33
Mutations:
None
Discrepancy:
t1223a,F408Y
                                

5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : INTS4
Symbol Nomenclature : INTS4
SYNONYM : INT4; MST093
Designation : MSTP093
Full Nomenclature : integrator complex subunit 4
GENEID : 92105
Locus Tag : MSTP093
HGNC : 25048
MIM : 611348
Vega : OTTHUMG00000166629
Target GenBank: BC009995

 

Reference Sequence Alignment

  

HsCD00329788      ATGGCGGCGCACCTTAAGAAGCGGGTTTATGAGGAATTCACGAAAGTGGTTCAGCCACAG
NM_033547.3       ATGGCGGCGCACCTTAAGAAGCGGGTTTATGAGGAATTCACGAAAGTGGTTCAGCCACAG
                  ************************************************************

HsCD00329788      GAGGAAATTGCTACTAAGAAACTCCGACTAACAAAACCAAGTAAATCTGCAGCACTCCAC
NM_033547.3       GAGGAAATTGCTACTAAGAAACTCCGACTAACAAAACCAAGTAAATCTGCAGCACTCCAC
                  ************************************************************

HsCD00329788      ATAGATCTGTGTAAAGCTACCTCCCCAGCAGATGCTTTGCAATACTTGCTCCAGTTTGCC
NM_033547.3       ATAGATCTGTGTAAAGCTACCTCCCCAGCAGATGCTTTGCAATACTTGCTCCAGTTTGCC
                  ************************************************************

HsCD00329788      AGGAAGCCTGTCGAGGCGGAAAGCGTAGAGGGAGTAGTCAGGATTCTCTTGGAACATTAT
NM_033547.3       AGGAAGCCTGTCGAGGCGGAAAGCGTAGAGGGAGTAGTCAGGATTCTCTTGGAACATTAT
                  ************************************************************

HsCD00329788      TACAAGGAGAATGATCCATCTGTGAGACTGAAAATTGCATCATTGTTGGGTTTATTATCA
NM_033547.3       TACAAGGAGAATGATCCATCTGTGAGACTGAAAATTGCATCATTGTTGGGTTTATTATCA
                  ************************************************************

HsCD00329788      AAGACAGCAGGATTTTCACCAGACTGCATTATGGATGATGCCATCAACATCCTGCAGAAT
NM_033547.3       AAGACAGCAGGATTTTCACCAGACTGCATTATGGATGATGCCATCAACATCCTGCAGAAT
                  ************************************************************

HsCD00329788      GAAAAGTCTCATCAAGTCCTAGCTCAACTGCTGGATACTTTGCTTGCAATTGGCACTAAG
NM_033547.3       GAAAAGTCTCATCAAGTCCTAGCTCAACTGCTGGATACTTTGCTTGCAATTGGCACTAAG
                  ************************************************************

HsCD00329788      CTACCAGAGAATCAAGCTATCCAAATGCGATTAGTTGATGTGGCCTGCAAGCATCTGACA
NM_033547.3       CTACCAGAGAATCAAGCTATCCAAATGCGATTAGTTGATGTGGCCTGCAAGCATCTGACA
                  ************************************************************

HsCD00329788      GATACGTCTCATGGTGTAAGAAATAAGTGCCTGCAGTTACTTGGCAATCTTGGCTCTTTG
NM_033547.3       GATACGTCTCATGGTGTAAGAAATAAGTGCCTGCAGTTACTTGGCAATCTTGGCTCTTTG
                  ************************************************************

HsCD00329788      GAGAAAAGTGTCACAAAAGATGCAGAAGGCCTAGCTGCCAGAGATGTCCAGAAGATTATA
NM_033547.3       GAGAAAAGTGTCACAAAAGATGCAGAAGGCCTAGCTGCCAGAGATGTCCAGAAGATTATA
                  ************************************************************

HsCD00329788      GGGGATTACTTCAGTGACCAAGACCCACGTGTCAGAACAGCAGCTATAAAAGCCATGTTG
NM_033547.3       GGGGATTACTTCAGTGACCAAGACCCACGTGTCAGAACAGCAGCTATAAAAGCCATGTTG
                  ************************************************************

HsCD00329788      CAGCTCCATGAAAGAGGACTGAAATTACACCAAACAATTTATAATCAGGCCTGTAAATTA
NM_033547.3       CAGCTCCATGAAAGAGGACTGAAATTACACCAAACAATTTATAATCAGGCCTGTAAATTA
                  ************************************************************

HsCD00329788      CTCTCTGATGACTATGAACAAGTGCGCAGTGCTGCAGTCCAGCTTATCTGGGTCGTCAGT
NM_033547.3       CTCTCTGATGACTATGAACAAGTGCGCAGTGCTGCAGTCCAGCTTATCTGGGTCGTCAGT
                  ************************************************************

HsCD00329788      CAGCTCTATCCTGAAAGCATTGTCCCAATTCCTTCTTCTAATGAAGAAATACGCTTAGTT
NM_033547.3       CAGCTCTATCCTGAAAGCATTGTCCCAATTCCTTCTTCTAATGAAGAAATACGCTTAGTT
                  ************************************************************

HsCD00329788      GATGATGCGTTTGGCAAAATTTGTCACATGGTCAGTGATGGCTCTTGGGTGGTTCGTGTT
NM_033547.3       GATGATGCGTTTGGCAAAATTTGTCACATGGTCAGTGATGGCTCTTGGGTGGTTCGTGTT
                  ************************************************************

HsCD00329788      CAGGCAGCAAAACTGTTGGGCTCTATGGAGCAAGTCAGTTCTCATTTCTTGGAGCAGACC
NM_033547.3       CAGGCAGCAAAACTGTTGGGCTCTATGGAGCAAGTCAGTTCTCATTTCTTGGAGCAGACC
                  ************************************************************

HsCD00329788      CTTGACAAGAAGCTGATGTCAGATCTGAGGAGGAAACGTACTGCACATGAGCGTGCCAAG
NM_033547.3       CTTGACAAGAAGCTGATGTCAGATCTGAGGAGGAAACGTACTGCACATGAGCGTGCCAAG
                  ************************************************************

HsCD00329788      GAACTTTACAGTTCGGGGGAGTTTTCCAGTGGCAGAAAGTGGGGAGATGATGCTCCCAAG
NM_033547.3       GAACTTTACAGTTCGGGGGAGTTTTCCAGTGGCAGAAAGTGGGGAGATGATGCTCCCAAG
                  ************************************************************

HsCD00329788      GAAGAAGTAGATACCGGGGCTGTGAACTTGATTGAGTCAGGAGCTTGTGGAGCTTTTGTT
NM_033547.3       GAAGAAGTAGATACCGGGGCTGTGAACTTGATTGAGTCAGGAGCTTGTGGAGCTTTTGTT
                  ************************************************************

HsCD00329788      CATGGGTTGGAAGATGAGATGTATGAGGTTCGTATTGCTGCTGTGGAGGCCCTCTGCATG
NM_033547.3       CATGGGTTGGAAGATGAGATGTATGAGGTTCGTATTGCTGCTGTGGAGGCCCTCTGCATG
                  ************************************************************

HsCD00329788      TTGGCCCAGTCTTCACCCTCTTATGCTGAGAAGTGCCTTGATTTCCTAGTTGACATGTTC
NM_033547.3       TTGGCCCAGTCTTCACCCTCTTTTGCTGAGAAGTGCCTTGATTTCCTAGTTGACATGTTC
                  **********************:*************************************

HsCD00329788      AACGATGAAATTGAGGAAGTACGTCTGCAGTCTATACATACCATGAGAAAAATCTCTAAC
NM_033547.3       AACGATGAAATTGAGGAAGTACGTCTGCAGTCTATACATACCATGAGAAAAATCTCTAAC
                  ************************************************************

HsCD00329788      AACATCACCCTCCGAGAAGATCAGCTTGACACTGTCCTGGCTGTGCTAGAGGATTCATCC
NM_033547.3       AACATCACCCTCCGAGAAGATCAGCTTGACACTGTCCTGGCTGTGCTAGAGGATTCATCC
                  ************************************************************

HsCD00329788      AGAGATATTCGAGAGGCTCTTCATGAACTCTTATGCTGTACTAATGTTTCAACCAAAGAA
NM_033547.3       AGAGATATTCGAGAGGCTCTTCATGAACTCTTATGCTGTACTAATGTTTCAACCAAAGAA
                  ************************************************************

HsCD00329788      GGGATTCATCTTGCATTGGTGGAGCTGCTGAAAAATTTAACCAAGTACCCTACTGATAGG
NM_033547.3       GGGATTCATCTTGCATTGGTGGAGCTGCTGAAAAATTTAACCAAGTACCCTACTGATAGG
                  ************************************************************

HsCD00329788      GACTCCATATGGAAGTTG------------------------------------------
NM_033547.3       GACTCCATATGGAAGTGCTTGAAGTTTCTGGGAAGTCGGCATCCAACCCTGGTGCTTCCC
                  ****************                                            

HsCD00329788      ------------------------------------------------------------
NM_033547.3       TTGGTGCCAGAGCTTCTGAGCACCCACCCATTTTTTGACACAGCTGAACCAGACATGGAT
                                                                              

HsCD00329788      ------------------------------------------------------------
NM_033547.3       GATCCAGCTTATATTGCAGTTTTGGTACTTATTTTCAATGCTGCTAAAACCTGTCCAACA
                                                                              

HsCD00329788      ------------------------------------------------------------
NM_033547.3       ATGCCAGCATTGTTCTCAGATCACACCTTCAGGCACTATGCCTACCTCCGAGACAGTCTT
                                                                              

HsCD00329788      ------------------------------------------------------------
NM_033547.3       TCTCATCTTGTTCCTGCCTTGAGGTTACCAGGTAGAAAACTGGTGTCATCAGCTGTTTCT
                                                                              

HsCD00329788      ------------------------------------------------------------
NM_033547.3       CCCAGCATCATACCTCAAGAGGATCCTTCCCAGCAGTTCCTGCAGCAGAGCCTTGAAAGA
                                                                              

HsCD00329788      ------------------------------------------------------------
NM_033547.3       GTGTATAGTCTTCAGCACTTGGACCCTCAGGGAGCCCAGGAGCTGCTGGAATTCACCATC
                                                                              

HsCD00329788      ------------------------------------------------------------
NM_033547.3       AGGGATCTGCAAAGACTTGGAGAACTTCAATCTGAATTGGCAGGAGTAGCTGATTTCTCT
                                                                              

HsCD00329788      ------------------------------------------------------------
NM_033547.3       GCCACCTATCTTCGCTGTCAACTACTTCTCATCAAGGCCTTGCAGGAAAAGTTGTGGAAT
                                                                              

HsCD00329788      ------------------------------------------------------------
NM_033547.3       GTAGCTGCCCCTTTGTATTTGAAGCAGAGTGATTTGGCCTCAGCAGCAGCGAAACAGATT
                                                                              

HsCD00329788      ------------------------------------------------------------
NM_033547.3       ATGGAAGAGACCTACAAAATGGAATTCATGTACAGTGGTGTGGAGAATAAGCAGGTGGTG
                                                                              

HsCD00329788      ------------------------------------------------------------
NM_033547.3       ATTATACATCACATGAGGCTGCAGGCCAAAGCTTTGCAACTTATAGTAACAGCACGAACT
                                                                              

HsCD00329788      ------------------------------------------------------------
NM_033547.3       ACACGAGGACTTGACCCCTTATTTGGGATGTGTGAAAAATTTTTACAGGAAGTAGACTTT
                                                                              

HsCD00329788      ------------------------------------------------------------
NM_033547.3       TTTCAGAGGTATTTCATCGCTGATTTGCCCCACTTGCAGGACAGCTTTGTGGACAAACTC
                                                                              

HsCD00329788      ------------------------------------------------------------
NM_033547.3       CTTGACCTTATGCCCCGACTCATGACATCCAAACCTGCAGAAGTGGTCAAAATTCTACAG
                                                                              

HsCD00329788      ------------------------------------------------------------
NM_033547.3       ACCATGCTGCGACAGAGTGCCTTTCTGCATCTCCCGCTTCCAGAGCAGATCCACAAAGCC
                                                                              

HsCD00329788      ------------------------------------------------------------
NM_033547.3       TCAGCCACCATCATCGAGCCAGCGGGCGAGTCAGACAACCCTTTGCGGTTTACCTCTGGG
                                                                              

HsCD00329788      ------------------------------------------------------------
NM_033547.3       TTGGTGGTTGCCCTGGATGTTGATGCAACCCTGGAGCATGTGCAGGATCCTCAGAACACT
                                                                              

HsCD00329788      ------------------------------------------------------------
NM_033547.3       GTTAAGGTCCAGGTCTTATATCCAGATGGCCAGGCTCAGATGATTCACCCCAAGCCTGCA
                                                                              

HsCD00329788      ------------------------------------------------------------
NM_033547.3       GACTTCCGGAATCCTGGCCCAGGGCGGCACCGGCTCATCACTCAGGTTTATCTCTCCCAC
                                                                              

HsCD00329788      ------------------------------------------------------------
NM_033547.3       ACCGCTTGGACAGAGGCATGCCAGGTGGAAGTGAGGCTGCTGCTGGCCTACAACTCCAGT
                                                                              

HsCD00329788      ------------------------------------------------------------
NM_033547.3       GCTCGCATTCCAAAATGCCCCTGGATGGAGGGTGGTGAGATGTCACCACAGGTGGAAACC
                                                                              

HsCD00329788      ------------------------------------------------------------
NM_033547.3       AGCATCGAGGGCACCATTCCCTTCAGCAAGCCTGTAAAAGTTTATATAATGCCCAAACCT
                                                                              

HsCD00329788      ------------
NM_033547.3       GCACGGCGCTAA
                              

Protein

UniProt : Q96HW7
HPRD : 12386

Clone

Cloning Information : 268529
HIP Master Clone ID : 194781
Original Clone ID : FLH268529.01L

Organism


TAX_ID : 9606
Species Specific ID: 92105

Genome

Chromosome : 11
Map Location : 11q14.1
Ensembl : ENSG00000149262

Reagents

Labome : INTS4-antibody