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ZNF397 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  ZNF397
Gene Name:  zinc finger protein 397
Sequence             
Original Clone ID: FLH268530.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329789

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 828nts         Open reading frame : 1 to 828
Reference Sequence Alignment

ATGGCTGTGGAATCTGGAGTGATTTCAACCCTGATACCTCAGGATCCTCCGGAACAAGAA
CTAATACTAGTGAAAGTAGAAGATAACTTTTCCTGGGATGAGAAATTTAAGCAGAATGGG
AGTACTCAATCCTGCCAAGAATTGTTTCGTCAGCAATTCAGAAAATTTTGCTACCAGGAG
ACACCTGGGCCCCGGGAGGCTCTGAGCCGACTCCAGGAACTTTGCTATCAGTGGCTAATG
CCAGAGTTGCACACAAAGGAGCAGATCTTAGAACTGCTGGTACTGGAGCAGTTCCTGAGC
ATTCTGCCTGAGGAGCTGCAGATCTGGGTTCAGCAACATAATCCAGAAAGCGGCGAGGAA
GCTGTGACCCTGTTGGAGGATTTAGAGAGGGAGTTTGATGACCCAGGGCAGCAGGTCCCA
GCTAGTCCACAGGGACCAGCAGTGCCATGGAAGGATTTAACATGTCTCAGAGCATCCCAA
GAGTCAACAGACATCCACCTCCAGCCCTTAAAGACACAGCTGAAATCCTGGAAACCATGC
CTTTCCCCTAAAAGTGATTGTGAGAACAGTGAAACAGCAACAAAAGAGGGCATCTCAGAA
GAAAAATCACAGGGACTCCCTCAGGAACCTTCATTTCGAGGAATTAAGTTGTCCAGACCT
CCCAAAGCTTCTTCAGCTATCCGTTGGGAATGTGTTTCTCCAGGAAGTTTTCCCGGCGAT
ATCATAGCTGCTGAGGCTACACATTCAACAATTTCTTGCTTTGCCATCAACACTTTGCCA
GCCACCATCCTGCCATCTAAAAATGTGAATAGAAAGTATTTTTCCTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 963
Start on reference sequence 136
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ZNF397
Symbol Nomenclature : ZNF397
SYNONYM : ZNF47; ZSCAN15
Designation : zinc finger and SCAN domain-containing protein 15|zinc finger protein 47
Full Nomenclature : zinc finger protein 397
GENEID : 84307
HGNC : 18818
MIM : 609601
Vega : OTTHUMG00000178532
Target GenBank: BC006172

 

Reference Sequence Alignment

  

HsCD00329789      ATGGCTGTGGAATCTGGAGTGATTTCAACCCTGATACCTCAGGATCCTCCGGAACAAGAA
NM_032347.2       ATGGCTGTGGAATCTGGAGTGATTTCAACCCTGATACCTCAGGATCCTCCGGAACAAGAA
                  ************************************************************

HsCD00329789      CTAATACTAGTGAAAGTAGAAGATAACTTTTCCTGGGATGAGAAATTTAAGCAGAATGGG
NM_032347.2       CTAATACTAGTGAAAGTAGAAGATAACTTTTCCTGGGATGAGAAATTTAAGCAGAATGGG
                  ************************************************************

HsCD00329789      AGTACTCAATCCTGCCAAGAATTGTTTCGTCAGCAATTCAGAAAATTTTGCTACCAGGAG
NM_032347.2       AGTACTCAATCCTGCCAAGAATTGTTTCGTCAGCAATTCAGAAAATTTTGCTACCAGGAG
                  ************************************************************

HsCD00329789      ACACCTGGGCCCCGGGAGGCTCTGAGCCGACTCCAGGAACTTTGCTATCAGTGGCTAATG
NM_032347.2       ACACCTGGGCCCCGGGAGGCTCTGAGCCGACTCCAGGAACTTTGCTATCAGTGGCTAATG
                  ************************************************************

HsCD00329789      CCAGAGTTGCACACAAAGGAGCAGATCTTAGAACTGCTGGTACTGGAGCAGTTCCTGAGC
NM_032347.2       CCAGAGTTGCACACAAAGGAGCAGATCTTAGAACTGCTGGTACTGGAGCAGTTCCTGAGC
                  ************************************************************

HsCD00329789      ATTCTGCCTGAGGAGCTGCAGATCTGGGTTCAGCAACATAATCCAGAAAGCGGCGAGGAA
NM_032347.2       ATTCTGCCTGAGGAGCTGCAGATCTGGGTTCAGCAACATAATCCAGAAAGCGGCGAGGAA
                  ************************************************************

HsCD00329789      GCTGTGACCCTGTTGGAGGATTTAGAGAGGGAGTTTGATGACCCAGGGCAGCAGGTCCCA
NM_032347.2       GCTGTGACCCTGTTGGAGGATTTAGAGAGGGAGTTTGATGACCCAGGGCAGCAGGTCCCA
                  ************************************************************

HsCD00329789      GCTAGTCCACAGGGACCAGCAGTGCCATGGAAGGATTTAACATGTCTCAGAGCATCCCAA
NM_032347.2       GCTAGTCCACAGGGACCAGCAGTGCCATGGAAGGATTTAACATGTCTCAGAGCATCCCAA
                  ************************************************************

HsCD00329789      GAGTCAACAGACATCCACCTCCAGCCCTTAAAGACACAGCTGAAATCCTGGAAACCATGC
NM_032347.2       GAGTCAACAGACATCCACCTCCAGCCCTTAAAGACACAGCTGAAATCCTGGAAACCATGC
                  ************************************************************

HsCD00329789      CTTTCCCCTAAAAGTGATTGTGAGAACAGTGAAACAGCAACAAAAGAGGGCATCTCAGAA
NM_032347.2       CTTTCCCCTAAAAGTGATTGTGAGAACAGTGAAACAGCAACAAAAGAGGGCATCTCAGAA
                  ************************************************************

HsCD00329789      GAAAAATCACAGGGACTCCCTCAGGAACCTTCATTTCGAGGAATTAAGTTGTCCAGACCT
NM_032347.2       GAAAAATCACAGGGACTCCCTCAGGAACCTTCATTTCGAGGAATTAAGTTGTCCAGACCT
                  ************************************************************

HsCD00329789      CCCAAAGCTTCTTCAGCTATCCGTTGGGAATGTGTTTCTCCAGGAAGTTTTCCCGGCGAT
NM_032347.2       CCCAAAGCTTCTTCAGCTATCCGTTGGGAATGTGTTTCTCCAGGAAGTTTTCCCGGCGAT
                  ************************************************************

HsCD00329789      ATCATAGCTGCTGAGGCTACACATTCAACAATTTCTTGCTTTGCCATCAACACTTTGCCA
NM_032347.2       ATCATAGCTGCTGAGGCTACACATTCAACAATTTCTTGCTTTGCCATCAACACTTTGCCA
                  ************************************************************

HsCD00329789      GCCACCATCCTGCCATCTAAAAATGTGAATAGAAAGTATTTTTCCTTG
NM_032347.2       GCCACCATCCTGCCATCTAAAAATGTGAATAGAAAGTATTTTTCCTGA
                  ********************************************** .

Protein

SMART domain : SCAN : leucine rich region
UniProt : Q8NF99
HPRD : 18335

Clone

Cloning Information : 268530
HIP Master Clone ID : 194782
Original Clone ID : FLH268530.01L

Organism


TAX_ID : 9606
Species Specific ID: 84307

Genome

Chromosome : 18
Map Location : 18q12.2
Ensembl : ENSG00000186812

Reagents

Labome : ZNF397-antibody