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GFI1 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  GFI1
Gene Name:  growth factor independent 1 transcription repressor
Sequence             
Original Clone ID: FLH269199.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329795

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1269nts         Open reading frame : 1 to 1269
Reference Sequence Alignment

ATGCCGCGCTCATTTCTCGTCAAAAGCAAGAAGGCTCACAGCTACCACCAGCCGCGCTCC
CCAGGACCAGACTATTCCCTCCGTTTAGAGAATGTACCGGCGCCTAGCCGAGCAGACAGC
ACTTCAAATGCAGGCGGGGCGAAGGCGGAGCCCCGGGACCGTTTGTCCCCCGAATCGCAG
CTGACCGAAGCCCCAGACAGAGCCTCCGCATCCCCAGACAGCTGCGAAGGCAGCGTCTGC
GAACGGAGCTCGGAGTTTGAGGACTTCTGGAGGCCCCCGTCACCCTCCGCGTCTCCAGCC
TCGGAGAAGTCAATGTGCCCATCGCTGGACGAAGCCCAGCCCTTCCCCCTGCCTTTCAAA
CCGTACTCATGGAGCGGCCTGGCGGGTTCTGACCTGCGGCACCTGGTGCAGAGCTACCGA
CCGTGTGGGGCCCTGGAGCGTGGCGCTGGCCTGGGCCTCTTCTGTGAACCCGCCCCGGAG
CCTGGCCACCCGGCCGCGCTGTACGGCCCGAAGCGGGCTGCCGGCGGCGCGGGGGCCGGG
GCGCCAGGGAGCTGCAGCGCAGGGGCCGGTGCCACCGCTGGCCCTGGCCTAGGGCTCTAC
GGCGACTTCGGGTCTGCGGCAGCCGGGCTGTATGAGAGGCCCACGGCAGCGGCGGGCTTG
CTGTACCCCGAGCGTGGCCACGGGCTGCACGCAGACAAGGGCGCTGGCGTCAAGGTGGAG
TCGGAGCTGCTGTGCACCCGCCTGCTGCTGGGCGGCGGCTCCTACAAGTGCATCAAGTGC
AGCAAGGTGTTCTCCACGCCGCACGGGCTCGAGGTGCACGTGCGCAGGTCCCACAGCGGT
ACCAGACCCTTTGCCTGCGAGATGTGCGGCAAGACCTTCGGGCACGCGGTGAGCCTGGAG
CAGCACAAAGCCGTGCACTCGCAGGAACGGAGCTTTGACTGTAAGATCTGTGGGAAGAGC
TTCAAGAGGTCATCCACACTGTCCACACACCTGCTTATCCACTCAGACACTCGGCCCTAC
CCCTGTCAGTACTGTGGCAAGAGGTTCCACCAGAAGTCAGACATGAAGAAACACACTTTC
ATCCACACTGGTGAGAAGCCTCACAAGTGCCAGGTGTGCGGCAAGGCATTCAGCCAGAGC
TCCAACCTCATCACCCACAGCCGCAAACACACAGGCTTCAAGCCCTTCGGCTGCGACCTC
TGTGGGAAGGGTTTCCAGAGGAAGGTGGACCTCCGAAGGCACCGGGAGACGCAGCATGGG
CTCAAATTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1403
Start on reference sequence 135
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : GFI1
Symbol Nomenclature : GFI1
SYNONYM : GFI-1; GFI1A; SCN2; ZNF163
Designation : growth factor independence-1|growth factor independent protein 1|zinc finger protein 163|zinc finger protein Gfi-1
Full Nomenclature : growth factor independent 1 transcription repressor
GENEID : 2672
HGNC : 4237
MIM : 600871
Vega : OTTHUMG00000010897
Target GenBank: BC032751

 

Reference Sequence Alignment

  

HsCD00329795        ATGCCGCGCTCATTTCTCGTCAAAAGCAAGAAGGCTCACAGCTACCACCAGCCGCGCTCC
NM_001127216.1      ATGCCGCGCTCATTTCTCGTCAAAAGCAAGAAGGCTCACAGCTACCACCAGCCGCGCTCC
NM_005263.3         ATGCCGCGCTCATTTCTCGTCAAAAGCAAGAAGGCTCACAGCTACCACCAGCCGCGCTCC
NM_001127215.1      ATGCCGCGCTCATTTCTCGTCAAAAGCAAGAAGGCTCACAGCTACCACCAGCCGCGCTCC
                    ************************************************************

HsCD00329795        CCAGGACCAGACTATTCCCTCCGTTTAGAGAATGTACCGGCGCCTAGCCGAGCAGACAGC
NM_001127216.1      CCAGGACCAGACTATTCCCTCCGTTTAGAGAATGTACCGGCGCCTAGCCGAGCAGACAGC
NM_005263.3         CCAGGACCAGACTATTCCCTCCGTTTAGAGAATGTACCGGCGCCTAGCCGAGCAGACAGC
NM_001127215.1      CCAGGACCAGACTATTCCCTCCGTTTAGAGAATGTACCGGCGCCTAGCCGAGCAGACAGC
                    ************************************************************

HsCD00329795        ACTTCAAATGCAGGCGGGGCGAAGGCGGAGCCCCGGGACCGTTTGTCCCCCGAATCGCAG
NM_001127216.1      ACTTCAAATGCAGGCGGGGCGAAGGCGGAGCCCCGGGACCGTTTGTCCCCCGAATCGCAG
NM_005263.3         ACTTCAAATGCAGGCGGGGCGAAGGCGGAGCCCCGGGACCGTTTGTCCCCCGAATCGCAG
NM_001127215.1      ACTTCAAATGCAGGCGGGGCGAAGGCGGAGCCCCGGGACCGTTTGTCCCCCGAATCGCAG
                    ************************************************************

HsCD00329795        CTGACCGAAGCCCCAGACAGAGCCTCCGCATCCCCAGACAGCTGCGAAGGCAGCGTCTGC
NM_001127216.1      CTGACCGAAGCCCCAGACAGAGCCTCCGCATCCCCAGACAGCTGCGAAGGCAGCGTCTGC
NM_005263.3         CTGACCGAAGCCCCAGACAGAGCCTCCGCATCCCCAGACAGCTGCGAAGGCAGCGTCTGC
NM_001127215.1      CTGACCGAAGCCCCAGACAGAGCCTCCGCATCCCCAGACAGCTGCGAAGGCAGCGTCTGC
                    ************************************************************

HsCD00329795        GAACGGAGCTCGGAGTTTGAGGACTTCTGGAGGCCCCCGTCACCCTCCGCGTCTCCAGCC
NM_001127216.1      GAACGGAGCTCGGAGTTTGAGGACTTCTGGAGGCCCCCGTCACCCTCCGCGTCTCCAGCC
NM_005263.3         GAACGGAGCTCGGAGTTTGAGGACTTCTGGAGGCCCCCGTCACCCTCCGCGTCTCCAGCC
NM_001127215.1      GAACGGAGCTCGGAGTTTGAGGACTTCTGGAGGCCCCCGTCACCCTCCGCGTCTCCAGCC
                    ************************************************************

HsCD00329795        TCGGAGAAGTCAATGTGCCCATCGCTGGACGAAGCCCAGCCCTTCCCCCTGCCTTTCAAA
NM_001127216.1      TCGGAGAAGTCAATGTGCCCATCGCTGGACGAAGCCCAGCCCTTCCCCCTGCCTTTCAAA
NM_005263.3         TCGGAGAAGTCAATGTGCCCATCGCTGGACGAAGCCCAGCCCTTCCCCCTGCCTTTCAAA
NM_001127215.1      TCGGAGAAGTCAATGTGCCCATCGCTGGACGAAGCCCAGCCCTTCCCCCTGCCTTTCAAA
                    ************************************************************

HsCD00329795        CCGTACTCATGGAGCGGCCTGGCGGGTTCTGACCTGCGGCACCTGGTGCAGAGCTACCGA
NM_001127216.1      CCGTACTCATGGAGCGGCCTGGCGGGTTCTGACCTGCGGCACCTGGTGCAGAGCTACCGA
NM_005263.3         CCGTACTCATGGAGCGGCCTGGCGGGTTCTGACCTGCGGCACCTGGTGCAGAGCTACCGA
NM_001127215.1      CCGTACTCATGGAGCGGCCTGGCGGGTTCTGACCTGCGGCACCTGGTGCAGAGCTACCGA
                    ************************************************************

HsCD00329795        CCGTGTGGGGCCCTGGAGCGTGGCGCTGGCCTGGGCCTCTTCTGTGAACCCGCCCCGGAG
NM_001127216.1      CCGTGTGGGGCCCTGGAGCGTGGCGCTGGCCTGGGCCTCTTCTGCGAACCCGCCCCGGAG
NM_005263.3         CCGTGTGGGGCCCTGGAGCGTGGCGCTGGCCTGGGCCTCTTCTGCGAACCCGCCCCGGAG
NM_001127215.1      CCGTGTGGGGCCCTGGAGCGTGGCGCTGGCCTGGGCCTCTTCTGCGAACCCGCCCCGGAG
                    ******************************************** ***************

HsCD00329795        CCTGGCCACCCGGCCGCGCTGTACGGCCCGAAGCGGGCTGCCGGCGGCGCGGGGGCCGGG
NM_001127216.1      CCTGGCCACCCGGCCGCGCTGTACGGCCCGAAGCGGGCTGCCGGCGGCGCGGGGGCCGGG
NM_005263.3         CCTGGCCACCCGGCCGCGCTGTACGGCCCGAAGCGGGCTGCCGGCGGCGCGGGGGCCGGG
NM_001127215.1      CCTGGCCACCCGGCCGCGCTGTACGGCCCGAAGCGGGCTGCCGGCGGCGCGGGGGCCGGG
                    ************************************************************

HsCD00329795        GCGCCAGGGAGCTGCAGCGCAGGGGCCGGTGCCACCGCTGGCCCTGGCCTAGGGCTCTAC
NM_001127216.1      GCGCCAGGGAGCTGCAGCGCAGGGGCCGGTGCCACCGCTGGCCCTGGCCTAGGGCTCTAC
NM_005263.3         GCGCCAGGGAGCTGCAGCGCAGGGGCCGGTGCCACCGCTGGCCCTGGCCTAGGGCTCTAC
NM_001127215.1      GCGCCAGGGAGCTGCAGCGCAGGGGCCGGTGCCACCGCTGGCCCTGGCCTAGGGCTCTAC
                    ************************************************************

HsCD00329795        GGCGACTTCGGGTCTGCGGCAGCCGGGCTGTATGAGAGGCCCACGGCAGCGGCGGGCTTG
NM_001127216.1      GGCGACTTCGGGTCTGCGGCAGCCGGGCTGTATGAGAGGCCCACGGCAGCGGCGGGCTTG
NM_005263.3         GGCGACTTCGGGTCTGCGGCAGCCGGGCTGTATGAGAGGCCCACGGCAGCGGCGGGCTTG
NM_001127215.1      GGCGACTTCGGGTCTGCGGCAGCCGGGCTGTATGAGAGGCCCACGGCAGCGGCGGGCTTG
                    ************************************************************

HsCD00329795        CTGTACCCCGAGCGTGGCCACGGGCTGCACGCAGACAAGGGCGCTGGCGTCAAGGTGGAG
NM_001127216.1      CTGTACCCCGAGCGTGGCCACGGGCTGCACGCAGACAAGGGCGCTGGCGTCAAGGTGGAG
NM_005263.3         CTGTACCCCGAGCGTGGCCACGGGCTGCACGCAGACAAGGGCGCTGGCGTCAAGGTGGAG
NM_001127215.1      CTGTACCCCGAGCGTGGCCACGGGCTGCACGCAGACAAGGGCGCTGGCGTCAAGGTGGAG
                    ************************************************************

HsCD00329795        TCGGAGCTGCTGTGCACCCGCCTGCTGCTGGGCGGCGGCTCCTACAAGTGCATCAAGTGC
NM_001127216.1      TCGGAGCTGCTGTGCACCCGCCTGCTGCTGGGCGGCGGCTCCTACAAGTGCATCAAGTGC
NM_005263.3         TCGGAGCTGCTGTGCACCCGCCTGCTGCTGGGCGGCGGCTCCTACAAGTGCATCAAGTGC
NM_001127215.1      TCGGAGCTGCTGTGCACCCGCCTGCTGCTGGGCGGCGGCTCCTACAAGTGCATCAAGTGC
                    ************************************************************

HsCD00329795        AGCAAGGTGTTCTCCACGCCGCACGGGCTCGAGGTGCACGTGCGCAGGTCCCACAGCGGT
NM_001127216.1      AGCAAGGTGTTCTCCACGCCGCACGGGCTCGAGGTGCACGTGCGCAGGTCCCACAGCGGT
NM_005263.3         AGCAAGGTGTTCTCCACGCCGCACGGGCTCGAGGTGCACGTGCGCAGGTCCCACAGCGGT
NM_001127215.1      AGCAAGGTGTTCTCCACGCCGCACGGGCTCGAGGTGCACGTGCGCAGGTCCCACAGCGGT
                    ************************************************************

HsCD00329795        ACCAGACCCTTTGCCTGCGAGATGTGCGGCAAGACCTTCGGGCACGCGGTGAGCCTGGAG
NM_001127216.1      ACCAGACCCTTTGCCTGCGAGATGTGCGGCAAGACCTTCGGGCACGCGGTGAGCCTGGAG
NM_005263.3         ACCAGACCCTTTGCCTGCGAGATGTGCGGCAAGACCTTCGGGCACGCGGTGAGCCTGGAG
NM_001127215.1      ACCAGACCCTTTGCCTGCGAGATGTGCGGCAAGACCTTCGGGCACGCGGTGAGCCTGGAG
                    ************************************************************

HsCD00329795        CAGCACAAAGCCGTGCACTCGCAGGAACGGAGCTTTGACTGTAAGATCTGTGGGAAGAGC
NM_001127216.1      CAGCACAAAGCCGTGCACTCGCAGGAACGGAGCTTTGACTGTAAGATCTGTGGGAAGAGC
NM_005263.3         CAGCACAAAGCCGTGCACTCGCAGGAACGGAGCTTTGACTGTAAGATCTGTGGGAAGAGC
NM_001127215.1      CAGCACAAAGCCGTGCACTCGCAGGAACGGAGCTTTGACTGTAAGATCTGTGGGAAGAGC
                    ************************************************************

HsCD00329795        TTCAAGAGGTCATCCACACTGTCCACACACCTGCTTATCCACTCAGACACTCGGCCCTAC
NM_001127216.1      TTCAAGAGGTCATCCACACTGTCCACACACCTGCTTATCCACTCAGACACTCGGCCCTAC
NM_005263.3         TTCAAGAGGTCATCCACACTGTCCACACACCTGCTTATCCACTCAGACACTCGGCCCTAC
NM_001127215.1      TTCAAGAGGTCATCCACACTGTCCACACACCTGCTTATCCACTCAGACACTCGGCCCTAC
                    ************************************************************

HsCD00329795        CCCTGTCAGTACTGTGGCAAGAGGTTCCACCAGAAGTCAGACATGAAGAAACACACTTTC
NM_001127216.1      CCCTGTCAGTACTGTGGCAAGAGGTTCCACCAGAAGTCAGACATGAAGAAACACACTTTC
NM_005263.3         CCCTGTCAGTACTGTGGCAAGAGGTTCCACCAGAAGTCAGACATGAAGAAACACACTTTC
NM_001127215.1      CCCTGTCAGTACTGTGGCAAGAGGTTCCACCAGAAGTCAGACATGAAGAAACACACTTTC
                    ************************************************************

HsCD00329795        ATCCACACTGGTGAGAAGCCTCACAAGTGCCAGGTGTGCGGCAAGGCATTCAGCCAGAGC
NM_001127216.1      ATCCACACTGGTGAGAAGCCTCACAAGTGCCAGGTGTGCGGCAAGGCATTCAGCCAGAGC
NM_005263.3         ATCCACACTGGTGAGAAGCCTCACAAGTGCCAGGTGTGCGGCAAGGCATTCAGCCAGAGC
NM_001127215.1      ATCCACACTGGTGAGAAGCCTCACAAGTGCCAGGTGTGCGGCAAGGCATTCAGCCAGAGC
                    ************************************************************

HsCD00329795        TCCAACCTCATCACCCACAGCCGCAAACACACAGGCTTCAAGCCCTTCGGCTGCGACCTC
NM_001127216.1      TCCAACCTCATCACCCACAGCCGCAAACACACAGGCTTCAAGCCCTTCGGCTGCGACCTC
NM_005263.3         TCCAACCTCATCACCCACAGCCGCAAACACACAGGCTTCAAGCCCTTCGGCTGCGACCTC
NM_001127215.1      TCCAACCTCATCACCCACAGCCGCAAACACACAGGCTTCAAGCCCTTCGGCTGCGACCTC
                    ************************************************************

HsCD00329795        TGTGGGAAGGGTTTCCAGAGGAAGGTGGACCTCCGAAGGCACCGGGAGACGCAGCATGGG
NM_001127216.1      TGTGGGAAGGGTTTCCAGAGGAAGGTGGACCTCCGAAGGCACCGGGAGACGCAGCATGGG
NM_005263.3         TGTGGGAAGGGTTTCCAGAGGAAGGTGGACCTCCGAAGGCACCGGGAGACGCAGCATGGG
NM_001127215.1      TGTGGGAAGGGTTTCCAGAGGAAGGTGGACCTCCGAAGGCACCGGGAGACGCAGCATGGG
                    ************************************************************

HsCD00329795        CTCAAATTG
NM_001127216.1      CTCAAATGA
NM_005263.3         CTCAAATGA
NM_001127215.1      CTCAAATGA
                    ******* .

Pathways

NCI : Validated targets of C-MYC transcriptional repression

Protein

SMART domain : ZnF_C2H2 : zinc finger
UniProt : B3KVN4
UniProt : Q99684
HPRD : 07527

Clone

Cloning Information : 269199
HIP Master Clone ID : 144097
Original Clone ID : FLH269199.01L

Organism


TAX_ID : 9606
Species Specific ID: 2672

Genome

Chromosome : 1
Map Location : 1p22
Ensembl : ENSG00000162676

Reagents

Labome : GFI1-antibody