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TRIM22 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  TRIM22
Gene Name:  tripartite motif containing 22
Sequence             
Original Clone ID: FLH269201.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329797

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1497nts         Open reading frame : 1 to 1497
Reference Sequence Alignment

ATGGATTTCTCAGTAAAGGTAGACATAGAGAAGGAGGTGACCTGCCCCATCTGCCTGGAG
CTCCTGACAGAACCTCTGAGCCTAGATTGTGGCCACAGCTTCTGCCAAGCCTGCATCACT
GCAAAGATCAAGGAGTCAGTGATCATCTCAAGAGGGGAAAGCAGCTGTCCTGTGTGTCAG
ACCAGATTCCAGCCTGGGAACCTCCGACCTAATCGGCATCTGGCCAACATAGTTGAGAGA
GTCAAAGAGGTCAAGATGAGCCCACAGGAGGGGCAGAAGAGAGATGTCTGTGAGCACCAT
GGAAAAAAACTCCAGATCTTCTGTAAGGAGGATGGAAAAGTCATTTGCTGGGTTTGTGAA
CTGTCTCAGGAACACCAAGGTCACCAAACATTCCGCATAAACGAGGTGGTCAAGGAATGT
CAGGAAAAGCTGCAGGTAGCCCTGCAGAGGCTGATAAAGGAGGATCAAGAGGCTGAGAAG
CTGGAAGATGACATCAGACAAGAGAGAACCGCCTGGAAGAATTATATCCAGATCGAGAGA
CAGAAGATTCTGAAAGGGTTCAATGAAATGAGAGTCATCTTGGACAATGAGGAGCAGAGA
GAGCTGCAAAAGCTGGAGGAAGGTGAGGTGAATGTGCTGGACAACCTGGCAGCAGCTACA
GACCAGCTGGTCCAGCAGAGGCAGGATGCCAGCACGCTCATCTCAGATCTCCAGCGGAGG
TTGAGGGGATCGTCAGTAGAGATGCTGCAGGATGTGATTGACGTCATGAAAAGGAGTGAA
AGCTGGACATTGAAGAAGCCAAAATCTGTTTCCAAGAAACTAAAGAGTGTATTCCGAGTA
CCAGATCTGAGTGGGATGCTGCAAGTTCTTAAAGAGCTGACAGATGTCCAGTACTACTGG
GTGGACGTGATGCTGAATCCAGGCAGTGCCACTTCGAATGTTGCTATTTCTGTGGATCAG
AGACAAGTGAAAACTGTACGCACCTGCACATTTAAGAATTCAAATCCATGTGATTTTTCT
GCTTTTGGTGTCTTCGGCTGCCAATATTTCTCTTCGGGGAAATATTACTGGGAAGTAGAT
GTGTCTGGAAAGATTGCCTGGATCCTGGGCGTACACAGTAAAATAAGTAGTCTGAATAAA
AGGAAGAGCTCTGGGTTTGCTTTTGATCCAAGTGTAAATTATTCAAAAGTTTACTCCAGA
TATAGACCTCAATATGGCTACTGGGTTATAGGATTACAGAATACATGTGAATATAATGCT
TTTGAGGACTCCTCCTCTTCTGATCCCAAGGTTTTGACTCTCTTTATGGCTGTGCCTCCC
TGTCGTATTGGGGTTTTCCTAGACTATGAGGCAGGCATTGTCTCATTTTTCAATGTCACA
AACCACGGAGCACTCATCTACAAGTTCTCTGGATGTCGCTTTTCTCGACCTGCTTATCCG
TATTTCAATCCTTGGAACTGCCTAGTCCCCATGACTGTGTGCCCACCGAGCTCCTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1649
Start on reference sequence 153
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : TRIM22
Symbol Nomenclature : TRIM22
SYNONYM : GPSTAF50; RNF94; STAF50
Designation : 50 kDa-stimulated trans-acting factor|E3 ubiquitin-protein ligase TRIM22|RING finger protein 94|staf-50|stimulated trans-acting factor (50 kDa)|tripartite binding motif 22|tripartite motif protein TRIM22|tripartite motif-containing 22|tripartite motif-containing protein 22
Full Nomenclature : tripartite motif containing 22
GENEID : 10346
HGNC : 16379
MIM : 606559
Vega : OTTHUMG00000066904
Target GenBank: BC035582

 

Reference Sequence Alignment

  

HsCD00329797        ATGGATTTCTCAGTAAAGGTAGACATAGAGAAGGAGGTGACCTGCCCCATCTGCCTGGAG
NM_006074.4         ATGGATTTCTCAGTAAAGGTAGACATAGAGAAGGAGGTGACCTGCCCCATCTGCCTGGAG
NM_001199573.1      ATGGATTTCTCAGTAAAGGTAGACATAGAGAAGGAGGTGACCTGCCCCATCTGCCTGGAG
                    ************************************************************

HsCD00329797        CTCCTGACAGAACCTCTGAGCCTAGATTGTGGCCACAGCTTCTGCCAAGCCTGCATCACT
NM_006074.4         CTCCTGACAGAACCTCTGAGCCTAGATTGTGGCCACAGCTTCTGCCAAGCCTGCATCACT
NM_001199573.1      CTCCTGACAGAACCTCTGAGCCTAGATTGTGGCCACAGCTTCTGCCAAGCCTGCATCACT
                    ************************************************************

HsCD00329797        GCAAAGATCAAGGAGTCAGTGATCATCTCAAGAGGGGAAAGCAGCTGTCCTGTGTGTCAG
NM_006074.4         GCAAAGATCAAGGAGTCAGTGATCATCTCAAGAGGGGAAAGCAGCTGTCCTGTGTGTCAG
NM_001199573.1      GCAAAGATCAAGGAGTCAGTGATCATCTCAAGAGGGGAAAGCAGCTGTCCTGTGTGTCAG
                    ************************************************************

HsCD00329797        ACCAGATTCCAGCCTGGGAACCTCCGACCTAATCGGCATCTGGCCAACATAGTTGAGAGA
NM_006074.4         ACCAGATTCCAGCCTGGGAACCTCCGACCTAATCGGCATCTGGCCAACATAGTTGAGAGA
NM_001199573.1      ACCAGATTCCAGCCTGGGAACCTCCGACCTAATCGGCATCTGGCCAACATAGTTGAGAGA
                    ************************************************************

HsCD00329797        GTCAAAGAGGTCAAGATGAGCCCACAGGAGGGGCAGAAGAGAGATGTCTGTGAGCACCAT
NM_006074.4         GTCAAAGAGGTCAAGATGAGCCCACAGGAGGGGCAGAAGAGAGATGTCTGTGAGCACCAT
NM_001199573.1      GTCAAAGAGGTCAAGATGAGCCCACAGGAGGGGCAGAAGAGAGATGTCTGTGAGCACCAT
                    ************************************************************

HsCD00329797        GGAAAAAAACTCCAGATCTTCTGTAAGGAGGATGGAAAAGTCATTTGCTGGGTTTGTGAA
NM_006074.4         GGAAAAAAACTCCAGATCTTCTGTAAGGAGGATGGAAAAGTCATTTGCTGGGTTTGTGAA
NM_001199573.1      GGAAAAAAACTCCAGATCTTCTGTAAGGAGGATGGAAAAGTCATTTGCTGGGTTTGTGAA
                    ************************************************************

HsCD00329797        CTGTCTCAGGAACACCAAGGTCACCAAACATTCCGCATAAACGAGGTGGTCAAGGAATGT
NM_006074.4         CTGTCTCAGGAACACCAAGGTCACCAAACATTCCGCATAAACGAGGTGGTCAAGGAATGT
NM_001199573.1      CTGTCTCAGGAACACCAAGGTCACCAAACATTCCGCATAAACGAGGTGGTCAAGGAATGT
                    ************************************************************

HsCD00329797        CAGGAAAAGCTGCAGGTAGCCCTGCAGAGGCTGATAAAGGAGGATCAAGAGGCTGAGAAG
NM_006074.4         CAGGAAAAGCTGCAGGTAGCCCTGCAGAGGCTGATAAAGGAGGATCAAGAGGCTGAGAAG
NM_001199573.1      CAGGAAAAGCTGCAGGTAGCCCTGCAGAGGCTGATAAAGGAGGATCAAGAGGCTGAGAAG
                    ************************************************************

HsCD00329797        CTGGAAGATGACATCAGACAAGAGAGAACCGCCTGGAAGAATTATATCCAGATCGAGAGA
NM_006074.4         CTGGAAGATGACATCAGACAAGAGAGAACCGCCTGGAAGAATTATATCCAGATCGAGAGA
NM_001199573.1      CTGGAAGATGACATCAGACAAGAGAGAACCGCCTG------------GAAGATCGAGAGA
                    ***********************************             .***********

HsCD00329797        CAGAAGATTCTGAAAGGGTTCAATGAAATGAGAGTCATCTTGGACAATGAGGAGCAGAGA
NM_006074.4         CAGAAGATTCTGAAAGGGTTCAATGAAATGAGAGTCATCTTGGACAATGAGGAGCAGAGA
NM_001199573.1      CAGAAGATTCTGAAAGGGTTCAATGAAATGAGAGTCATCTTGGACAATGAGGAGCAGAGA
                    ************************************************************

HsCD00329797        GAGCTGCAAAAGCTGGAGGAAGGTGAGGTGAATGTGCTGGACAACCTGGCAGCAGCTACA
NM_006074.4         GAGCTGCAAAAGCTGGAGGAAGGTGAGGTGAATGTGCTGGATAACCTGGCAGCAGCTACA
NM_001199573.1      GAGCTGCAAAAGCTGGAGGAAGGTGAGGTGAATGTGCTGGATAACCTGGCAGCAGCTACA
                    ***************************************** ******************

HsCD00329797        GACCAGCTGGTCCAGCAGAGGCAGGATGCCAGCACGCTCATCTCAGATCTCCAGCGGAGG
NM_006074.4         GACCAGCTGGTCCAGCAGAGGCAGGATGCCAGCACGCTCATCTCAGATCTCCAGCGGAGG
NM_001199573.1      GACCAGCTGGTCCAGCAGAGGCAGGATGCCAGCACGCTCATCTCAGATCTCCAGCGGAGG
                    ************************************************************

HsCD00329797        TTGAGGGGATCGTCAGTAGAGATGCTGCAGGATGTGATTGACGTCATGAAAAGGAGTGAA
NM_006074.4         TTGAGGGGATCGTCAGTAGAGATGCTGCAGGATGTGATTGACGTCATGAAAAGGAGTGAA
NM_001199573.1      TTGAGGGGATCGTCAGTAGAGATGCTGCAGGATGTGATTGACGTCATGAAAAGGAGTGAA
                    ************************************************************

HsCD00329797        AGCTGGACATTGAAGAAGCCAAAATCTGTTTCCAAGAAACTAAAGAGTGTATTCCGAGTA
NM_006074.4         AGCTGGACATTGAAGAAGCCAAAATCTGTTTCCAAGAAACTAAAGAGTGTATTCCGAGTA
NM_001199573.1      AGCTGGACATTGAAGAAGCCAAAATCTGTTTCCAAGAAACTAAAGAGTGTATTCCGAGTA
                    ************************************************************

HsCD00329797        CCAGATCTGAGTGGGATGCTGCAAGTTCTTAAAGAGCTGACAGATGTCCAGTACTACTGG
NM_006074.4         CCAGATCTGAGTGGGATGCTGCAAGTTCTTAAAGAGCTGACAGATGTCCAGTACTACTGG
NM_001199573.1      CCAGATCTGAGTGGGATGCTGCAAGTTCTTAAAGAGCTGACAGATGTCCAGTACTACTGG
                    ************************************************************

HsCD00329797        GTGGACGTGATGCTGAATCCAGGCAGTGCCACTTCGAATGTTGCTATTTCTGTGGATCAG
NM_006074.4         GTGGACGTGATGCTGAATCCAGGCAGTGCCACTTCGAATGTTGCTATTTCTGTGGATCAG
NM_001199573.1      GTGGACGTGATGCTGAATCCAGGCAGTGCCACTTCGAATGTTGCTATTTCTGTGGATCAG
                    ************************************************************

HsCD00329797        AGACAAGTGAAAACTGTACGCACCTGCACATTTAAGAATTCAAATCCATGTGATTTTTCT
NM_006074.4         AGACAAGTGAAAACTGTACGCACCTGCACATTTAAGAATTCAAATCCATGTGATTTTTCT
NM_001199573.1      AGACAAGTGAAAACTGTACGCACCTGCACATTTAAGAATTCAAATCCATGTGATTTTTCT
                    ************************************************************

HsCD00329797        GCTTTTGGTGTCTTCGGCTGCCAATATTTCTCTTCGGGGAAATATTACTGGGAAGTAGAT
NM_006074.4         GCTTTTGGTGTCTTCGGCTGCCAATATTTCTCTTCGGGGAAATATTACTGGGAAGTAGAT
NM_001199573.1      GCTTTTGGTGTCTTCGGCTGCCAATATTTCTCTTCGGGGAAATATTACTGGGAAGTAGAT
                    ************************************************************

HsCD00329797        GTGTCTGGAAAGATTGCCTGGATCCTGGGCGTACACAGTAAAATAAGTAGTCTGAATAAA
NM_006074.4         GTGTCTGGAAAGATTGCCTGGATCCTGGGCGTACACAGTAAAATAAGTAGTCTGAATAAA
NM_001199573.1      GTGTCTGGAAAGATTGCCTGGATCCTGGGCGTACACAGTAAAATAAGTAGTCTGAATAAA
                    ************************************************************

HsCD00329797        AGGAAGAGCTCTGGGTTTGCTTTTGATCCAAGTGTAAATTATTCAAAAGTTTACTCCAGA
NM_006074.4         AGGAAGAGCTCTGGGTTTGCTTTTGATCCAAGTGTAAATTATTCAAAAGTTTACTCCAGA
NM_001199573.1      AGGAAGAGCTCTGGGTTTGCTTTTGATCCAAGTGTAAATTATTCAAAAGTTTACTCCAGA
                    ************************************************************

HsCD00329797        TATAGACCTCAATATGGCTACTGGGTTATAGGATTACAGAATACATGTGAATATAATGCT
NM_006074.4         TATAGACCTCAATATGGCTACTGGGTTATAGGATTACAGAATACATGTGAATATAATGCT
NM_001199573.1      TATAGACCTCAATATGGCTACTGGGTTATAGGATTACAGAATACATGTGAATATAATGCT
                    ************************************************************

HsCD00329797        TTTGAGGACTCCTCCTCTTCTGATCCCAAGGTTTTGACTCTCTTTATGGCTGTGCCTCCC
NM_006074.4         TTTGAGGACTCCTCCTCTTCTGATCCCAAGGTTTTGACTCTCTTTATGGCTGTGCCTCCC
NM_001199573.1      TTTGAGGACTCCTCCTCTTCTGATCCCAAGGTTTTGACTCTCTTTATGGCTGTGCCTCCC
                    ************************************************************

HsCD00329797        TGTCGTATTGGGGTTTTCCTAGACTATGAGGCAGGCATTGTCTCATTTTTCAATGTCACA
NM_006074.4         TGTCGTATTGGGGTTTTCCTAGACTATGAGGCAGGCATTGTCTCATTTTTCAATGTCACA
NM_001199573.1      TGTCGTATTGGGGTTTTCCTAGACTATGAGGCAGGCATTGTCTCATTTTTCAATGTCACA
                    ************************************************************

HsCD00329797        AACCACGGAGCACTCATCTACAAGTTCTCTGGATGTCGCTTTTCTCGACCTGCTTATCCG
NM_006074.4         AACCACGGAGCACTCATCTACAAGTTCTCTGGATGTCGCTTTTCTCGACCTGCTTATCCG
NM_001199573.1      AACCACGGAGCACTCATCTACAAGTTCTCTGGATGTCGCTTTTCTCGACCTGCTTATCCG
                    ************************************************************

HsCD00329797        TATTTCAATCCTTGGAACTGCCTAGTCCCCATGACTGTGTGCCCACCGAGCTCCTTG
NM_006074.4         TATTTCAATCCTTGGAACTGCCTAGTCCCCATGACTGTGTGCCCACCGAGCTCCTGA
NM_001199573.1      TATTTCAATCCTTGGAACTGCCTAGTCCCCATGACTGTGTGCCCACCGAGCTCCTGA
                    ******************************************************* .

Protein

SMART domain : BBOX : B-Box-type zinc finger
SMART domain : SPRY : Domain in SPla and the RYanodine Receptor.
SMART domain : RING : Ring finger
UniProt : Q8IYM9
UniProt : B4DQS5
HPRD : 05951

Clone

Cloning Information : 269201
HIP Master Clone ID : 143574
Original Clone ID : FLH269201.01L

Organism


TAX_ID : 9606
Species Specific ID: 10346

Genome

Chromosome : 11
Map Location : 11p15
Ensembl : ENSG00000132274

Reagents

Labome : TRIM22-antibody