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KBTBD7 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  KBTBD7
Gene Name:  kelch repeat and BTB (POZ) domain containing 7
Sequence             
Original Clone ID: FLH269203.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329799

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 2055nts         Open reading frame : 1 to 2055
Reference Sequence Alignment

ATGCAGTCCCGGGAAGACGTCCCGCGCTCTCGCCGCCTCGCCAGTCCCCGTGGTGGGAGG
CGGCCCAAGAGGATTTCCAAGCCCTCGGTTTCGGCCTTTTTCACGGGTCCAGAGGAGTTA
AAGGACACGGCCCATTCTGCAGCCCTGCTGGCACAGCTCAAGTCCTTCTACGACGCGCGG
CTGCTGTGTGATGTGACCATCGAGGTGGTGACGCCTGGCAGCGGGCCTGGCACGGGTCGC
CTCTTTTCCTGCAATCGCAACGTGCTAGCAGCTGCGTGTCCCTACTTCAAGAGCATGTTC
ACAGGTGGCATGTACGAGAGCCAGCAGGCCAGCGTGACCATGCACGATGTGGACGCCGAG
TCCTTCGAGGTGTTGGTCGACTACTGCTACACGGGTCGTGTGTCTCTCAGTGAGGCCAAT
GTGCAGCGCCTGTACGCGGCCTCCGACATGCTACAGCTGGAATATGTGCGGGAAGCCTGT
GCCTCCTTCTTAGCCCGACGTCTTGACCTGACCAACTGCACCGCCATCCTCAAGTTTGCA
GACGCCTTCGACCATCACAAGCTTCGATCTCAGGCCCAGTCCTACATAGCTCACAACTTC
AAGCAGCTCAGCCGAATGGGTTCAATTCGGGAGGAGACTCTAGCAGATCTAACCCTGGCC
CAGCTGCTGGCTGTCCTACGCCTGGATAGTCTGGACATAGAGAGTGAGCGGACTGTATGC
CATGTAGCTGTGCAGTGGCTGGAGGCTGCTGCCAAAGAGCGGGGTCCCAGTGCTGCAGAA
GTCTTCAAGTGCGTGCGCTGGATGCACTTCACTGAAGAAGATCAGGACTACTTAGAAGGG
CTGCTGACCAAGCCCATCGTGAAGAAGTACTGCCTGGACGTTATTGAAGGGGCCCTGCAG
ATGCGCTATGGTGACCTGTTGTACAAGTCTCTGGTGCCAGTGCCAAACAGCAGCAGCAGC
AGTAGCAGCAGCAACTCTCTTGTATCTGCAGCAGAAAATCCACCCCAGAGACTGGGTATG
TGTGCCAAGGAGATGGTGATCTTCTTTGGACATCCTAGAGATCCCTTTCTCTGCTATGAC
CCTTACTCGGGGGACATTTACACAATGCCATCCCCTTTGACCAGCTTTGCTCACACTAAG
ACTGTCACCTCCTCAGCTGTCTGTGTGTCCCCAGACCATGACATCTATCTAGCTGCTCAG
CCCAGGAAAGACCTCTGGGTGTATAAACCAGCTCAGAATAGTTGGCAGCAACTTGCAGAT
CGCTTGCTGTGTCGTGAGGGCATGGATGTGGCATATCTCAATGGCTACATCTACATTTTG
GGGGGACGAGACCCTATTACTGGAGTTAAGTTGAAGGAAGTGGAATGCTACAGTGTTCAG
AGAAACCAGTGGGCATTGGTGGCTCCTGTCCCTCATTCCTTCTATTCCTTTGAACTCATA
GTGGTTCAGAACTATCTTTATGCTGTCAACAGTAAGCGCATGCTTTGCTATGATCCTAGC
CACAATATGTGGCTGAACTGTGCTTCTCTTAAACGTAGTGACTTTCAGGAAGCATGTGTC
TTCAATGATGAAATCTATTGTATCTGTGACATCCCAGTCATGAAGGTCTACAACCCAGCT
AGGGGAGAATGGAGGCGGATTAGTAATATTCCTTTGGATTCAGAGACCCACAACTACCAG
ATTGTCAATCATGACCAAAAGTTGCTTCTCATCACTTCTACAACCCCACAATGGAAAAAG
AACCGAGTGACAGTGTATGAGTATGATACTAGGGAAGATCAGTGGATTAATATAGGTACC
ATGTTAGGCCTTTTGCAGTTTGACTCTGGCTTTATTTGCCTTTGTGCTCGTGTTTATCCT
TCCTGCCTTGAACCTGGTCAGAGTTTTATTACTGAGGAAGATGATGCACGGAGTGAGTCT
AGTACTGAATGGGACTTAGATGGATTCAGTGAGCTGGACTCTGAGTCAGGAAGTTCAAGT
TCTTTTTCAGATGATGAAGTCTGGGTGCAAGTAGCACCTCAGCGAAATGCACAGGATCAG
CAGGGTTCTTTGTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 2369
Start on reference sequence 315
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : KBTBD7
Symbol Nomenclature : KBTBD7
Designation : kelch repeat and BTB domain-containing protein 7
Full Nomenclature : kelch repeat and BTB (POZ) domain containing 7
GENEID : 84078
HGNC : 25266
Target GenBank: BC022033

 

Reference Sequence Alignment

  

HsCD00329799      ATGCAGTCCCGGGAAGACGTCCCGCGCTCTCGCCGCCTCGCCAGTCCCCGTGGTGGGAGG
NM_032138.4       ATGCAGTCCCGGGAAGACGTCCCGCGCTCTCGCCGCCTCGCCAGTCCCCGTGGTGGGAGG
                  ************************************************************

HsCD00329799      CGGCCCAAGAGGATTTCCAAGCCCTCGGTTTCGGCCTTTTTCACGGGTCCAGAGGAGTTA
NM_032138.4       CGGCCCAAGAGGATTTCCAAGCCCTCGGTTTCGGCCTTTTTCACGGGTCCAGAGGAGTTA
                  ************************************************************

HsCD00329799      AAGGACACGGCCCATTCTGCAGCCCTGCTGGCACAGCTCAAGTCCTTCTACGACGCGCGG
NM_032138.4       AAGGACACGGCCCATTCTGCAGCCCTGCTGGCACAGCTCAAGTCCTTCTACGACGCGCGG
                  ************************************************************

HsCD00329799      CTGCTGTGTGATGTGACCATCGAGGTGGTGACGCCTGGCAGCGGGCCTGGCACGGGTCGC
NM_032138.4       CTGCTGTGTGATGTGACCATCGAGGTGGTGACGCCTGGCAGCGGGCCTGGCACGGGTCGC
                  ************************************************************

HsCD00329799      CTCTTTTCCTGCAATCGCAACGTGCTAGCAGCTGCGTGTCCCTACTTCAAGAGCATGTTC
NM_032138.4       CTCTTTTCCTGCAATCGCAACGTGCTAGCAGCTGCGTGTCCCTACTTCAAGAGCATGTTC
                  ************************************************************

HsCD00329799      ACAGGTGGCATGTACGAGAGCCAGCAGGCCAGCGTGACCATGCACGATGTGGACGCCGAG
NM_032138.4       ACAGGTGGCATGTACGAGAGCCAGCAGGCCAGCGTGACCATGCACGATGTGGACGCCGAG
                  ************************************************************

HsCD00329799      TCCTTCGAGGTGTTGGTCGACTACTGCTACACGGGTCGTGTGTCTCTCAGTGAGGCCAAT
NM_032138.4       TCCTTCGAGGTGTTGGTCGACTACTGCTACACGGGTCGTGTGTCTCTCAGTGAGGCCAAT
                  ************************************************************

HsCD00329799      GTGCAGCGCCTGTACGCGGCCTCCGACATGCTACAGCTGGAATATGTGCGGGAAGCCTGT
NM_032138.4       GTGCAGCGCCTGTACGCGGCCTCCGACATGCTACAGCTGGAATATGTGCGGGAAGCCTGT
                  ************************************************************

HsCD00329799      GCCTCCTTCTTAGCCCGACGTCTTGACCTGACCAACTGCACCGCCATCCTCAAGTTTGCA
NM_032138.4       GCCTCCTTCTTAGCCCGACGTCTTGACCTGACCAACTGCACCGCCATCCTCAAGTTTGCA
                  ************************************************************

HsCD00329799      GACGCCTTCGACCATCACAAGCTTCGATCTCAGGCCCAGTCCTACATAGCTCACAACTTC
NM_032138.4       GACGCCTTCGACCATCACAAGCTTCGATCTCAGGCCCAGTCCTACATAGCTCACAACTTC
                  ************************************************************

HsCD00329799      AAGCAGCTCAGCCGAATGGGTTCAATTCGGGAGGAGACTCTAGCAGATCTAACCCTGGCC
NM_032138.4       AAGCAGCTCAGCCGAATGGGTTCAATTCGGGAGGAGACTCTAGCAGATCTAACCCTGGCC
                  ************************************************************

HsCD00329799      CAGCTGCTGGCTGTCCTACGCCTGGATAGTCTGGACATAGAGAGTGAGCGGACTGTATGC
NM_032138.4       CAGCTGCTGGCTGTCCTACGCCTGGATAGTCTGGACATAGAGAGTGAGCGGACTGTATGC
                  ************************************************************

HsCD00329799      CATGTAGCTGTGCAGTGGCTGGAGGCTGCTGCCAAAGAGCGGGGTCCCAGTGCTGCAGAA
NM_032138.4       CATGTAGCTGTGCAGTGGCTGGAGGCTGCTGCCAAAGAGCGGGGTCCCAGTGCTGCAGAA
                  ************************************************************

HsCD00329799      GTCTTCAAGTGCGTGCGCTGGATGCACTTCACTGAAGAAGATCAGGACTACTTAGAAGGG
NM_032138.4       GTCTTCAAGTGCGTGCGCTGGATGCACTTCACTGAAGAAGATCAGGACTACTTAGAAGGG
                  ************************************************************

HsCD00329799      CTGCTGACCAAGCCCATCGTGAAGAAGTACTGCCTGGACGTTATTGAAGGGGCCCTGCAG
NM_032138.4       CTGCTGACCAAGCCCATCGTGAAGAAGTACTGCCTGGACGTTATTGAAGGGGCCCTGCAG
                  ************************************************************

HsCD00329799      ATGCGCTATGGTGACCTGTTGTACAAGTCTCTGGTGCCAGTGCCAAACAGCAGCAGCAGC
NM_032138.4       ATGCGCTATGGTGACCTGTTGTACAAGTCTCTGGTGCCAGTGCCAAACAGCAGCAGCAGC
                  ************************************************************

HsCD00329799      AGTAGCAGCAGCAACTCTCTTGTATCTGCAGCAGAAAATCCACCCCAGAGACTGGGTATG
NM_032138.4       AGTAGCAGCAGCAACTCTCTTGTATCTGCAGCAGAAAATCCACCCCAGAGACTGGGTATG
                  ************************************************************

HsCD00329799      TGTGCCAAGGAGATGGTGATCTTCTTTGGACATCCTAGAGATCCCTTTCTCTGCTATGAC
NM_032138.4       TGTGCCAAGGAGATGGTGATCTTCTTTGGACATCCTAGAGATCCCTTTCTCTGCTATGAC
                  ************************************************************

HsCD00329799      CCTTACTCGGGGGACATTTACACAATGCCATCCCCTTTGACCAGCTTTGCTCACACTAAG
NM_032138.4       CCTTACTCGGGGGACATTTACACAATGCCATCCCCTTTGACCAGCTTTGCTCACACTAAG
                  ************************************************************

HsCD00329799      ACTGTCACCTCCTCAGCTGTCTGTGTGTCCCCAGACCATGACATCTATCTAGCTGCTCAG
NM_032138.4       ACTGTCACCTCCTCAGCTGTCTGTGTGTCCCCAGACCATGACATCTATCTAGCTGCTCAG
                  ************************************************************

HsCD00329799      CCCAGGAAAGACCTCTGGGTGTATAAACCAGCTCAGAATAGTTGGCAGCAACTTGCAGAT
NM_032138.4       CCCAGGAAAGACCTCTGGGTGTATAAACCAGCTCAGAATAGTTGGCAGCAACTTGCAGAT
                  ************************************************************

HsCD00329799      CGCTTGCTGTGTCGTGAGGGCATGGATGTGGCATATCTCAATGGCTACATCTACATTTTG
NM_032138.4       CGCTTGCTGTGTCGTGAGGGCATGGATGTGGCATATCTCAATGGCTACATCTACATTTTG
                  ************************************************************

HsCD00329799      GGGGGACGAGACCCTATTACTGGAGTTAAGTTGAAGGAAGTGGAATGCTACAGTGTTCAG
NM_032138.4       GGGGGACGAGACCCTATTACTGGAGTTAAGTTGAAGGAAGTGGAATGCTACAGTGTTCAG
                  ************************************************************

HsCD00329799      AGAAACCAGTGGGCATTGGTGGCTCCTGTCCCTCATTCCTTCTATTCCTTTGAACTCATA
NM_032138.4       AGAAACCAGTGGGCATTGGTGGCTCCTGTCCCTCATTCCTTCTATTCCTTTGAACTCATA
                  ************************************************************

HsCD00329799      GTGGTTCAGAACTATCTTTATGCTGTCAACAGTAAGCGCATGCTTTGCTATGATCCTAGC
NM_032138.4       GTGGTTCAGAACTATCTTTATGCTGTCAACAGTAAGCGCATGCTTTGCTATGATCCTAGC
                  ************************************************************

HsCD00329799      CACAATATGTGGCTGAACTGTGCTTCTCTTAAACGTAGTGACTTTCAGGAAGCATGTGTC
NM_032138.4       CACAATATGTGGCTGAACTGTGCTTCTCTTAAACGTAGTGACTTTCAGGAAGCATGTGTC
                  ************************************************************

HsCD00329799      TTCAATGATGAAATCTATTGTATCTGTGACATCCCAGTCATGAAGGTCTACAACCCAGCT
NM_032138.4       TTCAATGATGAAATCTATTGTATCTGTGACATCCCAGTCATGAAGGTCTACAACCCAGCT
                  ************************************************************

HsCD00329799      AGGGGAGAATGGAGGCGGATTAGTAATATTCCTTTGGATTCAGAGACCCACAACTACCAG
NM_032138.4       AGGGGAGAATGGAGGCGGATTAGTAATATTCCTTTGGATTCAGAGACCCACAACTACCAG
                  ************************************************************

HsCD00329799      ATTGTCAATCATGACCAAAAGTTGCTTCTCATCACTTCTACAACCCCACAATGGAAAAAG
NM_032138.4       ATTGTCAATCATGACCAAAAGTTGCTTCTCATCACTTCTACAACCCCACAATGGAAAAAG
                  ************************************************************

HsCD00329799      AACCGAGTGACAGTGTATGAGTATGATACTAGGGAAGATCAGTGGATTAATATAGGTACC
NM_032138.4       AACCGAGTGACAGTGTATGAGTATGATACTAGGGAAGATCAGTGGATTAATATAGGTACC
                  ************************************************************

HsCD00329799      ATGTTAGGCCTTTTGCAGTTTGACTCTGGCTTTATTTGCCTTTGTGCTCGTGTTTATCCT
NM_032138.4       ATGTTAGGCCTTTTGCAGTTTGACTCTGGCTTTATTTGCCTTTGTGCTCGTGTTTATCCT
                  ************************************************************

HsCD00329799      TCCTGCCTTGAACCTGGTCAGAGTTTTATTACTGAGGAAGATGATGCACGGAGTGAGTCT
NM_032138.4       TCCTGCCTTGAACCTGGTCAGAGTTTTATTACTGAGGAAGATGATGCACGGAGTGAGTCT
                  ************************************************************

HsCD00329799      AGTACTGAATGGGACTTAGATGGATTCAGTGAGCTGGACTCTGAGTCAGGAAGTTCAAGT
NM_032138.4       AGTACTGAATGGGACTTAGATGGATTCAGTGAGCTGGACTCTGAGTCAGGAAGTTCAAGT
                  ************************************************************

HsCD00329799      TCTTTTTCAGATGATGAAGTCTGGGTGCAAGTAGCACCTCAGCGAAATGCACAGGATCAG
NM_032138.4       TCTTTTTCAGATGATGAAGTCTGGGTGCAAGTAGCACCTCAGCGAAATGCACAGGATCAG
                  ************************************************************

HsCD00329799      CAGGGTTCTTTGTTG
NM_032138.4       CAGGGTTCTTTGTAA
                  *************:.

Protein

SMART domain : BACK : BTB And C-terminal Kelch
SMART domain : BTB : Broad-Complex, Tramtrack and Bric a brac
SMART domain : Kelch :
UniProt : Q8WVZ9
HPRD : 13759

Clone

Cloning Information : 269203
HIP Master Clone ID : 139518
Original Clone ID : FLH269203.01L

Organism


TAX_ID : 9606
Species Specific ID: 84078

Genome

Chromosome : 13
Map Location : 13q14.11

Reagents

Labome : KBTBD7-antibody