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TCEB3 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  TCEB3
Gene Name:  transcription elongation factor B (SIII), polypeptide 3 (110kDa, elongin A)
Sequence             
Original Clone ID: FLH269204.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329800

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 2319nts         Open reading frame : 1 to 2319
Reference Sequence Alignment

ATGGCGGCGGAGTCGGCGCTCCAAGTTGTGGAGAAGCTGCAGGCGCGCCTGGCCGCGAAC
CCGGACCCTAAGAAGCTATTGAAATATTTGAAGAAACTCTCCACCCTGCCTATTACAGTA
GACATTCTTGCGGAGACTGGGGTTGGGAAAACAGTAAATAGCTTGCGAAAACACGAGCAT
GTTGGAAGCTTTGCCAGGGACCTAGTGGCCCAGTGGAAGAAGCTGGTTCCTGTGGAACGA
AATGCTGAGCCTGATGAACAGGACTTTGAGAAGAGCAATTCCCGAAAGCGCCCTCGGGAT
GCCCTGCAGAAGGAGGAGGAGATGGAGGGGGACTACCAAGAAACCTGGAAAGCCACGGGG
AGCCGATCCTATAGCCCTGACCACAGGCAGAAGAAACATAGGAAACTCTCGGAGCTCGAG
AGACCTCACAAAGTGTCTCACGGTCATGAGAGGAGAGATGAGAGAAAGAGGTGTCACAGA
ATGTCACCAACTTACTCTTCAGACCCTGAGTCTTCTGATTATGGCCATGTTCAATCCCCT
CCATCTTGTACCAGTCCTCATCAGATGTACGTCGACCACTACAGATCCCTGGAGGAGGAC
CAGGAGCCCATTGTTTCACACCAGAAGCCTGGGAAAGGCCACAGCAATGCCTTTCAGGAC
AGACTCGGGGCCAGCCAAGAACGACACCTGGGTGAACCCCATGGGAAAGGGGTTGTGAGT
CAAAACAAGGAGCACAAATCTTCCCACAAGGACAAACGCCCCGTGGATGCCAAGAGTGAT
GAGAAGGCCTCTGTGGTGAGCAGAGAGAAATCACACAAGGCCCTCTCCAAAGAGGAGAAC
CGAAGGCCACCCTCAGGGGACAATGCAAGGGAGAAACCGCCCTCTAGTGGCGTAAAGAAA
GAGAAGGACAGAGAGGGCAGCAGCCTGAAGAAGAAGTGTTTGCCTCCCTCAGAGGCCGCT
TCAGACAACCACCTGAAAAAGCCAAAGCACAGAGACCCAGAGAAAGCCAAATTGGACAAA
AGCAAGCAAGGTCTGGACAGCTTTGACACAGGAAAAGGAGCAGGAGACCTGTTGCCCAAG
GTAAAAGAGAAGGGTTCTAACAACCTAAAGACTCCAGAAGGGAAAGTCAAAACTAATTTG
GATAGAAAGTCACTGGGCTCCCTCCCTAAAGTTGAGGAGACAGATATGGAGGATGAATTC
GAGCAGCCAACCATGTCTTTTGAATCCTACCTCAGCTATGACCAGCCCCGGAAGAAAAAG
AAAAAGATTGTGAAAACTTCAGCCACGGCACTTGGAGATAAAGGACTTAAAAAAAATGAC
TCTAAAAGCACTGGTAAAAACTTGGACTCAGTTCAGAAATTACCCAAGGTGAACAAAACC
AAGTCAGAGAAGCCGGCTGGAGCTGATTTAGCCAAGCTGAGAAAGGTGCCTGATGTGTTG
CCAGTGTTGCCAGACCTCCCGTTACCCGCGATACAGGCCAATTACCGTCCACTGCCTTCC
CTCGAGCTGATATCCTCCTTCCAGCCAAAGCGAAAAGCGTTCTCTTCACCCCAGGAAGAA
GAAGAAGCTGGATTTACTGGGCGCAGAATGAATTCCAAGATGCAGGTGTATTCTGGTTCC
AAGTGTGCCTATCTCCCTAAAATGATGACCTTGCACCAGCAATGCATCCGAGTACTTAAA
AACAACATCGATTCAATCTTTGAAGTGGGAGGAGTCCCATACTCTGTTCTTGAACCCGTT
TTGGAGAGGTGTACACCTGATCAGCTGTATCGCATAGAGGAATACAATCATGTATTAATT
GAAGAAACAGATCAATTATGGAAAGTTCATTGTCACCGAGACTTTAAGGAAGAAAGACCC
GAAGAGTATGAGTCGTGGCGAGAGATGTACCTGCGGCTTCAGGACGCCCGAGAGCAGCGG
CTACGAGTACTAACAAAGAATATCCAGTTCGCACATGCCAATAAGCCCAAAGGCCGACAA
GCAAAGATGGCCTTTGTCAACTCTGTGGCCAAGCCACCTCGTGACGTCCGGAGGAGGCAG
GAAAAGTTTGGAACGGGAGGAGCAGCTGTCCCTGAGAAAATCAAGATCAAGCCAGCCCCG
TACCCCATGGGAAGCAGCCATGCTTCCGCCAGTAGTATCAGCTTTAACCCCAGCCCTGAG
GAGCCGGCCTATGATGGCCCAAGCACCAGCAGTGCCCACTTGGCACCAGTGGTCAGCAGC
ACTGTTTCCTATGATCCTAGGAAACCCACTGTGAAGAAAATTGCCCCAATGATGGCCAAG
ACAATTAAAGCTTTCAAGAACAGATTCTCCCGACGATTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 2350
Start on reference sequence 32
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : TCEB3
Symbol Nomenclature : TCEB3
SYNONYM : EloA; SIII; SIII p110; TCEB3A
Designation : RNA polymerase II transcription factor SIII subunit A1|elongin 110 kDa subunit|elongin-A|transcription elongation factor B alpha subunit|transcription elongation factor B polypeptide 3
Full Nomenclature : transcription elongation factor B (SIII), polypeptide 3 (110kDa, elongin A)
GENEID : 6924
Locus Tag : MSTP059
HGNC : 11620
MIM : 600786
Vega : OTTHUMG00000002957
Target GenBank: BC002883

 

Reference Sequence Alignment

  

HsCD00329800      ------------------------------------------------------------
NM_003198.2       ATGCACGGAGGGCGGAGCTGCGGCCCGAGGACGCGACGCGAGCCCAGTTCCGGCGAGGAG
                                                                              

HsCD00329800      ------------------ATGGCGGCGGAGTCGGCGCTCCAAGTTGTGGAGAAGCTGCAG
NM_003198.2       GCCGCGCCAGTGACAGCGATGGCGGCGGAGTCGGCGCTCCAAGTTGTGGAGAAGCTGCAG
                                    ******************************************

HsCD00329800      GCGCGCCTGGCCGCGAACCCGGACCCTAAGAAGCTATTGAAATATTTGAAGAAACTCTCC
NM_003198.2       GCGCGCCTGGCCGCGAACCCGGACCCTAAGAAGCTATTGAAATATTTGAAGAAACTCTCC
                  ************************************************************

HsCD00329800      ACCCTGCCTATTACAGTAGACATTCTTGCGGAGACTGGGGTTGGGAAAACAGTAAATAGC
NM_003198.2       ACCCTGCCTATTACAGTAGACATTCTTGCGGAGACTGGGGTTGGGAAAACAGTAAATAGC
                  ************************************************************

HsCD00329800      TTGCGAAAACACGAGCATGTTGGAAGCTTTGCCAGGGACCTAGTGGCCCAGTGGAAGAAG
NM_003198.2       TTGCGAAAACACGAGCATGTTGGAAGCTTTGCCAGGGACCTAGTGGCCCAGTGGAAGAAG
                  ************************************************************

HsCD00329800      CTGGTTCCTGTGGAACGAAATGCTGAGCCTGATGAACAGGACTTTGAGAAGAGCAATTCC
NM_003198.2       CTGGTTCCTGTGGAACGAAATGCTGAGCCTGATGAACAGGACTTTGAGAAGAGCAATTCC
                  ************************************************************

HsCD00329800      CGAAAGCGCCCTCGGGATGCCCTGCAGAAGGAGGAGGAGATGGAGGGGGACTACCAAGAA
NM_003198.2       CGAAAGCGCCCTCGGGATGCCCTGCAGAAGGAGGAGGAGATGGAGGGGGACTACCAAGAA
                  ************************************************************

HsCD00329800      ACCTGGAAAGCCACGGGGAGCCGATCCTATAGCCCTGACCACAGGCAGAAGAAACATAGG
NM_003198.2       ACCTGGAAAGCCACGGGGAGCCGATCCTATAGCCCTGACCACAGGCAGAAGAAACATAGG
                  ************************************************************

HsCD00329800      AAACTCTCGGAGCTCGAGAGACCTCACAAAGTGTCTCACGGTCATGAGAGGAGAGATGAG
NM_003198.2       AAACTCTCGGAGCTCGAGAGACCTCACAAAGTGTCTCACGGTCATGAGAGGAGAGATGAG
                  ************************************************************

HsCD00329800      AGAAAGAGGTGTCACAGAATGTCACCAACTTACTCTTCAGACCCTGAGTCTTCTGATTAT
NM_003198.2       AGAAAGAGGTGTCACAGAATGTCACCAACTTACTCTTCAGACCCTGAGTCTTCTGATTAT
                  ************************************************************

HsCD00329800      GGCCATGTTCAATCCCCTCCATCTTGTACCAGTCCTCATCAGATGTACGTCGACCACTAC
NM_003198.2       GGCCATGTTCAATCCCCTCCATCTTGTACCAGTCCTCATCAGATGTACGTCGACCACTAC
                  ************************************************************

HsCD00329800      AGATCCCTGGAGGAGGACCAGGAGCCCATTGTTTCACACCAGAAGCCTGGGAAAGGCCAC
NM_003198.2       AGATCCCTGGAGGAGGACCAGGAGCCCATTGTTTCACACCAGAAGCCTGGGAAAGGCCAC
                  ************************************************************

HsCD00329800      AGCAATGCCTTTCAGGACAGACTCGGGGCCAGCCAAGAACGACACCTGGGTGAACCCCAT
NM_003198.2       AGCAATGCCTTTCAGGACAGACTCGGGGCCAGCCAAGAACGACACCTGGGTGAACCCCAT
                  ************************************************************

HsCD00329800      GGGAAAGGGGTTGTGAGTCAAAACAAGGAGCACAAATCTTCCCACAAGGACAAACGCCCC
NM_003198.2       GGGAAAGGGGTTGTGAGTCAAAACAAGGAGCACAAATCTTCCCACAAGGACAAACGCCCC
                  ************************************************************

HsCD00329800      GTGGATGCCAAGAGTGATGAGAAGGCCTCTGTGGTGAGCAGAGAGAAATCACACAAGGCC
NM_003198.2       GTGGATGCCAAGAGTGATGAGAAGGCCTCTGTGGTGAGCAGAGAGAAATCACACAAGGCC
                  ************************************************************

HsCD00329800      CTCTCCAAAGAGGAGAACCGAAGGCCACCCTCAGGGGACAATGCAAGGGAGAAACCGCCC
NM_003198.2       CTCTCCAAAGAGGAGAACCGAAGGCCACCCTCAGGGGACAATGCAAGGGAGAAACCGCCC
                  ************************************************************

HsCD00329800      TCTAGTGGCGTAAAGAAAGAGAAGGACAGAGAGGGCAGCAGCCTGAAGAAGAAGTGTTTG
NM_003198.2       TCTAGTGGCGTAAAGAAAGAGAAGGACAGAGAGGGCAGCAGCCTGAAGAAGAAGTGTTTG
                  ************************************************************

HsCD00329800      CCTCCCTCAGAGGCCGCTTCAGACAACCACCTGAAAAAGCCAAAGCACAGAGACCCAGAG
NM_003198.2       CCTCCCTCAGAGGCCGCTTCAGACAACCACCTGAAAAAGCCAAAGCACAGAGACCCAGAG
                  ************************************************************

HsCD00329800      AAAGCCAAATTGGACAAAAGCAAGCAAGGTCTGGACAGCTTTGACACAGGAAAAGGAGCA
NM_003198.2       AAAGCCAAATTGGACAAAAGCAAGCAAGGTCTGGACAGCTTTGACACAGGAAAAGGAGCA
                  ************************************************************

HsCD00329800      GGAGACCTGTTGCCCAAGGTAAAAGAGAAGGGTTCTAACAACCTAAAGACTCCAGAAGGG
NM_003198.2       GGAGACCTGTTGCCCAAGGTAAAAGAGAAGGGTTCTAACAACCTAAAGACTCCAGAAGGG
                  ************************************************************

HsCD00329800      AAAGTCAAAACTAATTTGGATAGAAAGTCACTGGGCTCCCTCCCTAAAGTTGAGGAGACA
NM_003198.2       AAAGTCAAAACTAATTTGGATAGAAAGTCACTGGGCTCCCTCCCTAAAGTTGAGGAGACA
                  ************************************************************

HsCD00329800      GATATGGAGGATGAATTCGAGCAGCCAACCATGTCTTTTGAATCCTACCTCAGCTATGAC
NM_003198.2       GATATGGAGGATGAATTCGAGCAGCCAACCATGTCTTTTGAATCCTACCTCAGCTATGAC
                  ************************************************************

HsCD00329800      CAGCCCCGGAAGAAAAAGAAAAAGATTGTGAAAACTTCAGCCACGGCACTTGGAGATAAA
NM_003198.2       CAGCCCCGGAAGAAAAAGAAAAAGATTGTGAAAACTTCAGCCACGGCACTTGGAGATAAA
                  ************************************************************

HsCD00329800      GGACTTAAAAAAAATGACTCTAAAAGCACTGGTAAAAACTTGGACTCAGTTCAGAAATTA
NM_003198.2       GGACTTAAAAAAAATGACTCTAAAAGCACTGGTAAAAACTTGGACTCAGTTCAGAAATTA
                  ************************************************************

HsCD00329800      CCCAAGGTGAACAAAACCAAGTCAGAGAAGCCGGCTGGAGCTGATTTAGCCAAGCTGAGA
NM_003198.2       CCCAAGGTGAACAAAACCAAGTCAGAGAAGCCGGCTGGAGCTGATTTAGCCAAGCTGAGA
                  ************************************************************

HsCD00329800      AAGGTGCCTGATGTGTTGCCAGTGTTGCCAGACCTCCCGTTACCCGCGATACAGGCCAAT
NM_003198.2       AAGGTGCCTGATGTGTTGCCAGTGTTGCCAGACCTCCCGTTACCCGCGATACAGGCCAAT
                  ************************************************************

HsCD00329800      TACCGTCCACTGCCTTCCCTCGAGCTGATATCCTCCTTCCAGCCAAAGCGAAAAGCGTTC
NM_003198.2       TACCGTCCACTGCCTTCCCTCGAGCTGATATCCTCCTTCCAGCCAAAGCGAAAAGCGTTC
                  ************************************************************

HsCD00329800      TCTTCACCCCAGGAAGAAGAAGAAGCTGGATTTACTGGGCGCAGAATGAATTCCAAGATG
NM_003198.2       TCTTCACCCCAGGAAGAAGAAGAAGCTGGATTTACTGGGCGCAGAATGAATTCCAAGATG
                  ************************************************************

HsCD00329800      CAGGTGTATTCTGGTTCCAAGTGTGCCTATCTCCCTAAAATGATGACCTTGCACCAGCAA
NM_003198.2       CAGGTGTATTCTGGTTCCAAGTGTGCCTATCTCCCTAAAATGATGACCTTGCACCAGCAA
                  ************************************************************

HsCD00329800      TGCATCCGAGTACTTAAAAACAACATCGATTCAATCTTTGAAGTGGGAGGAGTCCCATAC
NM_003198.2       TGCATCCGAGTACTTAAAAACAACATCGATTCAATCTTTGAAGTGGGAGGAGTCCCATAC
                  ************************************************************

HsCD00329800      TCTGTTCTTGAACCCGTTTTGGAGAGGTGTACACCTGATCAGCTGTATCGCATAGAGGAA
NM_003198.2       TCTGTTCTTGAACCCGTTTTGGAGAGGTGTACACCTGATCAGCTGTATCGCATAGAGGAA
                  ************************************************************

HsCD00329800      TACAATCATGTATTAATTGAAGAAACAGATCAATTATGGAAAGTTCATTGTCACCGAGAC
NM_003198.2       TACAATCATGTATTAATTGAAGAAACAGATCAATTATGGAAAGTTCATTGTCACCGAGAC
                  ************************************************************

HsCD00329800      TTTAAGGAAGAAAGACCCGAAGAGTATGAGTCGTGGCGAGAGATGTACCTGCGGCTTCAG
NM_003198.2       TTTAAGGAAGAAAGACCCGAAGAGTATGAGTCGTGGCGAGAGATGTACCTGCGGCTTCAG
                  ************************************************************

HsCD00329800      GACGCCCGAGAGCAGCGGCTACGAGTACTAACAAAGAATATCCAGTTCGCACATGCCAAT
NM_003198.2       GACGCCCGAGAGCAGCGGCTACGAGTACTAACAAAGAATATCCAGTTCGCACATGCCAAT
                  ************************************************************

HsCD00329800      AAGCCCAAAGGCCGACAAGCAAAGATGGCCTTTGTCAACTCTGTGGCCAAGCCACCTCGT
NM_003198.2       AAGCCCAAAGGCCGACAAGCAAAGATGGCCTTTGTCAACTCTGTGGCCAAGCCACCTCGT
                  ************************************************************

HsCD00329800      GACGTCCGGAGGAGGCAGGAAAAGTTTGGAACGGGAGGAGCAGCTGTCCCTGAGAAAATC
NM_003198.2       GACGTCCGGAGGAGGCAGGAAAAGTTTGGAACGGGAGGAGCAGCTGTCCCTGAGAAAATC
                  ************************************************************

HsCD00329800      AAGATCAAGCCAGCCCCGTACCCCATGGGAAGCAGCCATGCTTCCGCCAGTAGTATCAGC
NM_003198.2       AAGATCAAGCCAGCCCCGTACCCCATGGGAAGCAGCCATGCTTCCGCCAGTAGCATCAGC
                  ***************************************************** ******

HsCD00329800      TTTAACCCCAGCCCTGAGGAGCCGGCCTATGATGGCCCAAGCACCAGCAGTGCCCACTTG
NM_003198.2       TTTAACCCCAGCCCTGAGGAGCCGGCCTATGATGGCCCAAGCACCAGCAGTGCCCACTTG
                  ************************************************************

HsCD00329800      GCACCAGTGGTCAGCAGCACTGTTTCCTATGATCCTAGGAAACCCACTGTGAAGAAAATT
NM_003198.2       GCACCAGTGGTCAGCAGCACTGTTTCCTATGATCCTAGGAAACCCACTGTGAAGAAAATT
                  ************************************************************

HsCD00329800      GCCCCAATGATGGCCAAGACAATTAAAGCTTTCAAGAACAGATTCTCCCGACGATTG
NM_003198.2       GCCCCAATGATGGCCAAGACAATTAAAGCTTTCAAGAACAGATTCTCCCGACGATAA
                  *******************************************************:.

Pathways

Reactome : Disease
Reactome : Elongation arrest and recovery
Reactome : Formation of HIV elongation complex in the absence of HIV Tat
Reactome : Formation of HIV-1 elongation complex containing HIV-1 Tat
Reactome : Formation of RNA Pol II elongation complex
Reactome : Gene Expression
Reactome : HIV Infection
Reactome : HIV Life Cycle
Reactome : HIV Transcription Elongation
Reactome : HIV elongation arrest and recovery
Reactome : Late Phase of HIV Life Cycle
Reactome : Pausing and recovery of HIV elongation
Reactome : Pausing and recovery of Tat-mediated HIV elongation
Reactome : RNA Polymerase II Pre-transcription Events
Reactome : RNA Polymerase II Transcription
Reactome : RNA Polymerase II Transcription Elongation
Reactome : Tat-mediated HIV elongation arrest and recovery
Reactome : Tat-mediated elongation of the HIV-1 transcript
Reactome : Transcription
Reactome : Transcription of the HIV genome

Clone

Cloning Information : 269204
HIP Master Clone ID : 166738
Original Clone ID : FLH269204.01L

Organism


TAX_ID : 9606
Species Specific ID: 6924

Genome

Chromosome : 1
Map Location : 1p36.1
Ensembl : ENSG00000011007

Reagents

Labome : TCEB3-antibody