DNASU Plasmid Repository • 480.965.5697 | Email

BTF3 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  BTF3
Gene Name:  basic transcription factor 3
Sequence             
Original Clone ID: FLH269205.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329801

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 489nts         Open reading frame : 1 to 489
Reference Sequence Alignment

ATGAAAGAAACAATCATGAACCAGGAAAAACTCGCCAAACTGCAGGCACAAGTGCGCATT
GGTGGGAAAGGAACTGCTCGCAGAAAGAAGAAGGTGGTTCATAGAACAGCCACAGCAGAT
GACAAAAAACTTCAGTTCTCCTTAAAGAAGTTAGGGGTAAACAATATCTCTGGTATTGAA
GAGGTGAATATGTTTACAAACCAAGGAACAGTGATCCACTTTAACAACCCTAAAGTTCAG
GCATCTCTGGCAGCGAACACTTTCACCATTACAGGCCATGCTGAGACAAAGCAGCTGACA
GAAATGCTACCCAGCATCTTAAACCAGCTTGGTGCGGATAGTCTGACTAGTTTAAGGAGA
CTGGCCGAAGCTCTGCCCAAACAATCTGTGGATGGAAAAGCACCACTTGCTACTGGAGAG
GATGATGATGATGAAGTTCCAGATCTTGTGGAGAATTTTGATGAGGCTTCCAAGAATGAG
GCAAACTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 600
Start on reference sequence 112
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : BTF3
Symbol Nomenclature : BTF3
SYNONYM : BETA-NAC; BTF3a; BTF3b; NACB
Designation : RNA polymerase B transcription factor 3|nascent-polypeptide-associated complex beta polypeptide|transcription factor BTF3
Full Nomenclature : basic transcription factor 3
GENEID : 689
Locus Tag : OK/SW-cl.8
HGNC : 1125
MIM : 602542
Vega : OTTHUMG00000102031
Target GenBank: BC008062

 

Reference Sequence Alignment

  

HsCD00329801        ------------------------------------------------------------
NM_001207.4         ------------------------------------------------------------
NM_001037637.1      ATGCGACGGACAGGCGCACCCGCTCAGGCTGACTCTCGGGGGCGAGGTCGAGCCAGGGGC
                                                                                

HsCD00329801        ------------------------------------------------------------
NM_001207.4         ------------------------------------------------------------
NM_001037637.1      GGCTGCCCTGGGGGCGAGGCGACGCTGTCTCAACCTCCACCTCGCGGCGGAACCCGAGGA
                                                                                

HsCD00329801        ------------ATGAAAGAAACAATCATGAACCAGGAAAAACTCGCCAAACTGCAGGCA
NM_001207.4         ------------ATGAAAGAAACAATCATGAACCAGGAAAAACTCGCCAAACTGCAGGCA
NM_001037637.1      CAGGAGCCTCAGATGAAAGAAACAATCATGAACCAGGAAAAACTCGCCAAACTGCAGGCA
                                ************************************************

HsCD00329801        CAAGTGCGCATTGGTGGGAAAGGAACTGCTCGCAGAAAGAAGAAGGTGGTTCATAGAACA
NM_001207.4         CAAGTGCGCATTGGTGGGAAAGGAACTGCTCGCAGAAAGAAGAAGGTGGTTCATAGAACA
NM_001037637.1      CAAGTGCGCATTGGTGGGAAAGGAACTGCTCGCAGAAAGAAGAAGGTGGTTCATAGAACA
                    ************************************************************

HsCD00329801        GCCACAGCAGATGACAAAAAACTTCAGTTCTCCTTAAAGAAGTTAGGGGTAAACAATATC
NM_001207.4         GCCACAGCAGATGACAAAAAACTTCAGTTCTCCTTAAAGAAGTTAGGGGTAAACAATATC
NM_001037637.1      GCCACAGCAGATGACAAAAAACTTCAGTTCTCCTTAAAGAAGTTAGGGGTAAACAATATC
                    ************************************************************

HsCD00329801        TCTGGTATTGAAGAGGTGAATATGTTTACAAACCAAGGAACAGTGATCCACTTTAACAAC
NM_001207.4         TCTGGTATTGAAGAGGTGAATATGTTTACAAACCAAGGAACAGTGATCCACTTTAACAAC
NM_001037637.1      TCTGGTATTGAAGAGGTGAATATGTTTACAAACCAAGGAACAGTGATCCACTTTAACAAC
                    ************************************************************

HsCD00329801        CCTAAAGTTCAGGCATCTCTGGCAGCGAACACTTTCACCATTACAGGCCATGCTGAGACA
NM_001207.4         CCTAAAGTTCAGGCATCTCTGGCAGCGAACACTTTCACCATTACAGGCCATGCTGAGACA
NM_001037637.1      CCTAAAGTTCAGGCATCTCTGGCAGCGAACACTTTCACCATTACAGGCCATGCTGAGACA
                    ************************************************************

HsCD00329801        AAGCAGCTGACAGAAATGCTACCCAGCATCTTAAACCAGCTTGGTGCGGATAGTCTGACT
NM_001207.4         AAGCAGCTGACAGAAATGCTACCCAGCATCTTAAACCAGCTTGGTGCGGATAGTCTGACT
NM_001037637.1      AAGCAGCTGACAGAAATGCTACCCAGCATCTTAAACCAGCTTGGTGCGGATAGTCTGACT
                    ************************************************************

HsCD00329801        AGTTTAAGGAGACTGGCCGAAGCTCTGCCCAAACAATCTGTGGATGGAAAAGCACCACTT
NM_001207.4         AGTTTAAGGAGACTGGCCGAAGCTCTGCCCAAACAATCTGTGGATGGAAAAGCACCACTT
NM_001037637.1      AGTTTAAGGAGACTGGCCGAAGCTCTGCCCAAACAATCTGTGGATGGAAAAGCACCACTT
                    ************************************************************

HsCD00329801        GCTACTGGAGAGGATGATGATGATGAAGTTCCAGATCTTGTGGAGAATTTTGATGAGGCT
NM_001207.4         GCTACTGGAGAGGATGATGATGATGAAGTTCCAGATCTTGTGGAGAATTTTGATGAGGCT
NM_001037637.1      GCTACTGGAGAGGATGATGATGATGAAGTTCCAGATCTTGTGGAGAATTTTGATGAGGCT
                    ************************************************************

HsCD00329801        TCCAAGAATGAGGCAAACTTG
NM_001207.4         TCCAAGAATGAGGCAAACTGA
NM_001037637.1      TCCAAGAATGAGGCAAACTGA
                    ******************* .

Protein

UniProt : P20290
HPRD : 03966

Clone

Cloning Information : 269205
HIP Master Clone ID : 166277
Original Clone ID : FLH269205.01L

Organism


TAX_ID : 9606
Species Specific ID: 689

Genome

Chromosome : 5
Map Location : 5q13.2
Ensembl : ENSG00000145741

Reagents

Labome : BTF3-antibody