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RING1 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  RING1
Gene Name:  ring finger protein 1
Sequence             
Original Clone ID: FLH269207.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329803

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1134nts         Open reading frame : 1 to 1134
Reference Sequence Alignment

ATGGATGGCACAGAGATTGCTGTTTCCCCTCGGTCACTGCATTCAGAACTCATGTGCCCT
ATCTGCCTGGACATGCTGAAGAATACGATGACCACCAAGGAGTGCCTCCACAGATTCTGC
TCTGACTGCATTGTCACAGCCCTACGGAGCGGGAACAAGGAGTGTCCTACCTGCCGAAAG
AAGCTGGTGTCCAAGCGATCCCTACGGCCAGACCCCAACTTTGATGCCCTGATCTCTAAG
ATCTATCCTAGCCGGGAGGAATACGAGGCCCATCAAGACCGAGTGCTTATCCGCCTGAGC
CGCCTGCACAACCAGCAGGCATTGAGCTCCAGCATTGAGGAGGGGCTACGCATGCAGGCC
ATGCACAGGGCCCAGCGTGTGAGGCGGCCGATACCAGGGTCAGATCAGACCACAACGATG
AGTGGGGGGGAAGGAGAGCCCGGGGAGGGAGAAGGGGATGGAGAAGATGTGAGCTCAGAC
TCCGCCCCTGACTCTGCCCCAGGCCCTGCTCCCAAGCGACCCCGTGGAGGGGGCGCAGGG
GGGAGCAGTGTAGGGACAGGGGGAGGCGGCACTGGTGGGGTGGGTGGGGGTGCCGGTTCG
GAAGACTCTGGTGACCGGGGAGGGACTCTGGGAGGGGGAACGCTGGGCCCCCCAAGCCCT
CCTGGGGCCCCCAGCCCCCCAGAGCCAGGTGGAGAAATTGAGCTCGTGTTCCGGCCCCAC
CCCCTGCTCGTGGAGAAGGGAGAATACTGCCAGACGAGGTATGTGAAGACAACTGGGAAT
GCCACAGTGGACCACCTCTCCAAGTACTTGGCCCTGCGCATTGCCCTCGAGCGGAGGCAA
CAGCAGGAAGCAGGGGAGCCAGGAGGGCCTGGAGGGGGCGCCTCTGACACCGGAGGACCT
GATGGGTGTGGCGGGGAGGGTGGGGGTGCCGGAGGAGGTGACGGTCCTGAGGAGCCTGCT
TTGCCCAGCCTGGAGGGCGTCAGTGAAAAGCAGTACACCATCTACATCGCACCTGGAGGC
GGGGCGTTCACGACGTTGAATGGCTCGCTGACCCTGGAGCTGGTGAATGAGAAATTCTGG
AAGGTGTCCCGGCCACTGGAGCTGTGCTATGCTCCCACCAAGGATCCAAAGTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1419
Start on reference sequence 286
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : RING1
Symbol Nomenclature : RING1
SYNONYM : RING1A; RNF1
Designation : E3 ubiquitin-protein ligase RING1|polycomb complex protein RING1|really interesting new gene 1 protein
Full Nomenclature : ring finger protein 1
GENEID : 6015
Locus Tag : DADB-100D22.6
HGNC : 10018
MIM : 602045
Vega : OTTHUMG00000031278
Target GenBank: BC002922

 

Reference Sequence Alignment

  

HsCD00329803      ------------------------------------------------------------
NM_002931.3       ATGACGACGCCGGCGAATGCCCAGAATGCCAGCAAAACGTGGGAACTGAGTCTGTATGAG
                                                                              

HsCD00329803      ---------------------------ATGGATGGCACAGAGATTGCTGTTTCCCCTCGG
NM_002931.3       CTGCACCGGACCCCGCAGGAAGCCATAATGGATGGCACAGAGATTGCTGTTTCCCCTCGG
                                             *********************************

HsCD00329803      TCACTGCATTCAGAACTCATGTGCCCTATCTGCCTGGACATGCTGAAGAATACGATGACC
NM_002931.3       TCACTGCATTCAGAACTCATGTGCCCTATCTGCCTGGACATGCTGAAGAATACGATGACC
                  ************************************************************

HsCD00329803      ACCAAGGAGTGCCTCCACAGATTCTGCTCTGACTGCATTGTCACAGCCCTACGGAGCGGG
NM_002931.3       ACCAAGGAGTGCCTCCACAGATTCTGCTCTGACTGCATTGTCACAGCCCTACGGAGCGGG
                  ************************************************************

HsCD00329803      AACAAGGAGTGTCCTACCTGCCGAAAGAAGCTGGTGTCCAAGCGATCCCTACGGCCAGAC
NM_002931.3       AACAAGGAGTGTCCTACCTGCCGAAAGAAGCTGGTGTCCAAGCGATCCCTACGGCCAGAC
                  ************************************************************

HsCD00329803      CCCAACTTTGATGCCCTGATCTCTAAGATCTATCCTAGCCGGGAGGAATACGAGGCCCAT
NM_002931.3       CCCAACTTTGATGCCCTGATCTCTAAGATCTATCCTAGCCGGGAGGAATACGAGGCCCAT
                  ************************************************************

HsCD00329803      CAAGACCGAGTGCTTATCCGCCTGAGCCGCCTGCACAACCAGCAGGCATTGAGCTCCAGC
NM_002931.3       CAAGACCGAGTGCTTATCCGCCTGAGCCGCCTGCACAACCAGCAGGCATTGAGCTCCAGC
                  ************************************************************

HsCD00329803      ATTGAGGAGGGGCTACGCATGCAGGCCATGCACAGGGCCCAGCGTGTGAGGCGGCCGATA
NM_002931.3       ATTGAGGAGGGGCTACGCATGCAGGCCATGCACAGGGCCCAGCGTGTGAGGCGGCCGATA
                  ************************************************************

HsCD00329803      CCAGGGTCAGATCAGACCACAACGATGAGTGGGGGGGAAGGAGAGCCCGGGGAGGGAGAA
NM_002931.3       CCAGGGTCAGATCAGACCACAACGATGAGTGGGGGGGAAGGAGAGCCCGGGGAGGGAGAA
                  ************************************************************

HsCD00329803      GGGGATGGAGAAGATGTGAGCTCAGACTCCGCCCCTGACTCTGCCCCAGGCCCTGCTCCC
NM_002931.3       GGGGATGGAGAAGATGTGAGCTCAGACTCCGCCCCTGACTCTGCCCCAGGCCCTGCTCCC
                  ************************************************************

HsCD00329803      AAGCGACCCCGTGGAGGGGGCGCAGGGGGGAGCAGTGTAGGGACAGGGGGAGGCGGCACT
NM_002931.3       AAGCGACCCCGTGGAGGGGGCGCAGGGGGGAGCAGTGTAGGGACAGGGGGAGGCGGCACT
                  ************************************************************

HsCD00329803      GGTGGGGTGGGTGGGGGTGCCGGTTCGGAAGACTCTGGTGACCGGGGAGGGACTCTGGGA
NM_002931.3       GGTGGGGTGGGTGGGGGTGCCGGTTCGGAAGACTCTGGTGACCGGGGAGGGACTCTGGGA
                  ************************************************************

HsCD00329803      GGGGGAACGCTGGGCCCCCCAAGCCCTCCTGGGGCCCCCAGCCCCCCAGAGCCAGGTGGA
NM_002931.3       GGGGGAACGCTGGGCCCCCCAAGCCCTCCTGGGGCCCCCAGCCCCCCAGAGCCAGGTGGA
                  ************************************************************

HsCD00329803      GAAATTGAGCTCGTGTTCCGGCCCCACCCCCTGCTCGTGGAGAAGGGAGAATACTGCCAG
NM_002931.3       GAAATTGAGCTCGTGTTCCGGCCCCACCCCCTGCTCGTGGAGAAGGGAGAATACTGCCAG
                  ************************************************************

HsCD00329803      ACGAGGTATGTGAAGACAACTGGGAATGCCACAGTGGACCACCTCTCCAAGTACTTGGCC
NM_002931.3       ACGAGGTATGTGAAGACAACTGGGAATGCCACAGTGGACCACCTCTCCAAGTACTTGGCC
                  ************************************************************

HsCD00329803      CTGCGCATTGCCCTCGAGCGGAGGCAACAGCAGGAAGCAGGGGAGCCAGGAGGGCCTGGA
NM_002931.3       CTGCGCATTGCCCTCGAGCGGAGGCAACAGCAGGAAGCAGGGGAGCCAGGAGGGCCTGGA
                  ************************************************************

HsCD00329803      GGGGGCGCCTCTGACACCGGAGGACCTGATGGGTGTGGCGGGGAGGGTGGGGGTGCCGGA
NM_002931.3       GGGGGCGCCTCTGACACCGGAGGACCTGATGGGTGTGGCGGGGAGGGTGGGGGTGCCGGA
                  ************************************************************

HsCD00329803      GGAGGTGACGGTCCTGAGGAGCCTGCTTTGCCCAGCCTGGAGGGCGTCAGTGAAAAGCAG
NM_002931.3       GGAGGTGACGGTCCTGAGGAGCCTGCTTTGCCCAGCCTGGAGGGCGTCAGTGAAAAGCAG
                  ************************************************************

HsCD00329803      TACACCATCTACATCGCACCTGGAGGCGGGGCGTTCACGACGTTGAATGGCTCGCTGACC
NM_002931.3       TACACCATCTACATCGCACCTGGAGGCGGGGCGTTCACGACGTTGAATGGCTCGCTGACC
                  ************************************************************

HsCD00329803      CTGGAGCTGGTGAATGAGAAATTCTGGAAGGTGTCCCGGCCACTGGAGCTGTGCTATGCT
NM_002931.3       CTGGAGCTGGTGAATGAGAAATTCTGGAAGGTGTCCCGGCCACTGGAGCTGTGCTATGCT
                  ************************************************************

HsCD00329803      CCCACCAAGGATCCAAAGTTG
NM_002931.3       CCCACCAAGGATCCAAAGTGA
                  ******************* .

Pathways

Reactome : Cellular Senescence
Reactome : Cellular responses to stress
Reactome : Oxidative Stress Induced Senescence
WikiPathway : Notch Signaling Pathway

Protein

SMART domain : RING : Ring finger
UniProt : A8JZZ0
UniProt : Q06587
HPRD : 03624

Clone

Cloning Information : 269207
HIP Master Clone ID : 166096
Original Clone ID : FLH269207.01L

Organism


TAX_ID : 9606
Species Specific ID: 6015

Genome

Chromosome : 6
Map Location : 6p21.3
Ensembl : ENSG00000204227

Reagents

Labome : RING1-antibody