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CITED1 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  CITED1
Gene Name:  Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 1
Sequence             
Original Clone ID: FLH269213.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329809

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 582nts         Open reading frame : 1 to 582
Reference Sequence Alignment

ATGCCAACAACGTCGAGGCCTGCACTTGATGTCAAGGGTGGCACCTCACCTGCGAAGGAG
GATGCCAACCAAGAGATGAGCTCCGTGGCCTACTCCAACCTTGCGGTGAAAGATCGCAAA
GCAGTGGCCATTCTGCACTACCCTGGGGTAGCCTCAAATGGAACCAAGGCCAGTGGGGCT
CCCACTAGTTCCTCGGGATCTCCAATAGGCTCTCCTACAACCACCCCTCCCACTAAACCC
CCATCCTTCAACCTGCACCCCGCCCCTCACTTGCTGGCTAGTATGCAGCTGCAGAAACTT
AATAGCCAGTATCAGGGGATGGCTGCTGCCACTCCAGGCCAACCCGGGGAGGCAGGACCC
CTGCAAAACTGGGACTTTGGGGCCCAGGCGGGAGGGGCAGAATCACTCTCTCCTTCTGCT
GGTGCCCAGAGCCCTGCTATCATCGATTCGGACCCAGTGGATGAGGAAGTGCTGATGTCG
CTGGTGGTGGAACTGGGGTTGGACCGAGCCAATGAGCTTCCGGAGCTGTGGCTGGGGCAG
AATGAGTTTGACTTCACTGCGGACTTTCCATCTAGCTGCTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1111
Start on reference sequence 530
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CITED1
Symbol Nomenclature : CITED1
SYNONYM : MSG1
Designation : cbp/p300-interacting transactivator 1|melanocyte-specific gene 1|melanocyte-specific protein 1
Full Nomenclature : Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 1
GENEID : 4435
HGNC : 1986
MIM : 300149
Vega : OTTHUMG00000021812
Target GenBank: BC004240

 

Reference Sequence Alignment

  

HsCD00329809        ------------------------------------------------------------
NM_001144886.1      ------------------------------------------------------------
NM_004143.3         ------------------------------------------------------------
NM_001144885.1      ATGGAACCATCCGCACAACAGCTCCAGCTGGCAGCATCACTTCCCGCCAATTTATCCAAC
NM_001144887.1      ------------------------------------------------------------
                                                                                

HsCD00329809        ------------------ATGCCAACAACGTCGAGGCCTGCACTTGATGTCAAGGGTGGC
NM_001144886.1      ------------------ATGCCAACAACGTCGAGGCCTGCACTTGATGTCAAGGGTGGC
NM_004143.3         ------------------ATGCCAACAACGTCGAGGCCTGCACTTGATGTCAAGGGTGGC
NM_001144885.1      TTCTGCCAAGGCTCTGAAATGCCAACAACGTCGAGGCCTGCACTTGATGTCAAGGGTGGC
NM_001144887.1      ------------------ATGCCAACAACGTCGAGGCCTGCACTTGATGTCAAGGGTGGC
                                      ******************************************

HsCD00329809        ACCTCACCTGCGAAGGAGGATGCCAACCAAGAGATGAGCTCCGTGGCCTACTCCAACCTT
NM_001144886.1      ACCTCACCTGCGAAGGAGGATGCCAACCAAGAGATGAGCTCCGTGGCCTACTCCAACCTT
NM_004143.3         ACCTCACCTGCGAAGGAGGATGCCAACCAAGAGATGAGCTCCGTGGCCTACTCCAACCTT
NM_001144885.1      ACCTCACCTGCGAAGGAGGATGCCAACCAAGAGATGAGCTCCGTGGCCTACTCCAACCTT
NM_001144887.1      ACCTCACCTGCGAAGGAGGATGCCAACCAAGAGATGAGCTCCGTGGCCTACTCCAACCTT
                    ************************************************************

HsCD00329809        GCGGTGAAAGATCGCAAAGCAGTGGCCATTCTGCACTACCCTGGGGTAGCCTCAAATGGA
NM_001144886.1      GCGGTGAAAGATCGCAAAGCAGTGGCCATTCTGCACTACCCTGGGGTAGCCTCAAATGGA
NM_004143.3         GCGGTGAAAGATCGCAAAGCAGTGGCCATTCTGCACTACCCTGGGGTAGCCTCAAATGGA
NM_001144885.1      GCGGTGAAAGATCGCAAAGCAGTGGCCATTCTGCACTACCCTGGGGTAGCCTCAAATGGA
NM_001144887.1      GCGGTGAAAGATCGCAAAGCAGTGGCCATTCTGCACTACCCTGGGGTAGCCTCAAATGGA
                    ************************************************************

HsCD00329809        ACCAAGGCCAGTGGGGCTCCCACTAGTTCCTCGGGATCTCCAATAGGCTCTCCTACAACC
NM_001144886.1      ACCAAGGCCAGTGGGGCTCCCACTAGTTCCTCGGGATCTCCAATAGGCTCTCCTACAACC
NM_004143.3         ACCAAGGCCAGTGGGGCTCCCACTAGTTCCTCGGGATCTCCAATAGGCTCTCCTACAACC
NM_001144885.1      ACCAAGGCCAGTGGGGCTCCCACTAGTTCCTCGGGATCTCCAATAGGCTCTCCTACAACC
NM_001144887.1      ACCAAGGCCAGTGGGGCTCCCACTAGTTCCTCGGGATCTCCAATAGGCTCTCCTACAACC
                    ************************************************************

HsCD00329809        ACCCCTCCCACTAAACCCCCATCCTTCAACCTGCACCCCGCCCCTCACTTGCTGGCTAGT
NM_001144886.1      ACCCCTCCCACTAAACCCCCATCCTTCAACCTGCACCCCGCCCCTCACTTGCTGGCTAGT
NM_004143.3         ACCCCTCCCACTAAACCCCCATCCTTCAACCTGCACCCCGCCCCTCACTTGCTGGCTAGT
NM_001144885.1      ACCCCTCCCACTAAACCCCCATCCTTCAACCTGCACCCCGCCCCTCACTTGCTGGCTAGT
NM_001144887.1      ACCCCTCCCACTAAACCCCCATCCTTCAACCTGCACCCCGCCCCTCACTTGCTGGCTAGT
                    ************************************************************

HsCD00329809        ATGCAGCTGCAGAAACTTAATAGCCAGTATCAGGGGATGGCTGCTGCCACTCCAGGCCAA
NM_001144886.1      ATGCACCTGCAGAAACTTAATAGCCAGTATCAGGGGATGGCTGCTGCCACTCCAGGCCAA
NM_004143.3         ATGCACCTGCAGAAACTTAATAGCCAGTATCAGGGGATGGCTGCTGCCACTCCAGGCCAA
NM_001144885.1      ATGCACCTGCAGAAACTTAATAGCCAGTATCAGGGGATGGCTGCTGCCACTCCAGGCCAA
NM_001144887.1      ATGCACCTGCAGAAACTTAATAGCCAGTATCAGGGGATGGCTGCTGCCACTCCAGGCCAA
                    ***** ******************************************************

HsCD00329809        CCCGGGGAGGCAGGACCCCTGCAAAACTGGGACTTTGGGGCCCAGGCGGGAGGGGCAGAA
NM_001144886.1      CCCGGGGAGGCAGGACCCCTGCAAAACTGGGACTTTGGGGCCCAGGCGGGAGGGGCAGAA
NM_004143.3         CCCGGGGAGGCAGGACCCCTGCAAAACTGGGACTTTGGGGCCCAGGCGGGAGGGGCAGAA
NM_001144885.1      CCCGGGGAGGCAGGACCCCTGCAAAACTGGGACTTTGGGGCCCAGGCGGGAGGGGCAGAA
NM_001144887.1      CCCGGGGAGGCAGGACCCCTGCAAAACTGGGACTTTGGGGCCCAGGCGGGAGGGGCAGAA
                    ************************************************************

HsCD00329809        TCACTCTCTCCTTCTGCTGGTGCCCAGAGCCCTGCTATCATCGATTCGGACCCAGTGGAT
NM_001144886.1      TCACTCTCTCCTTCTGCTGGTGCCCAGAGCCCTGCTATCATCGATTCGGACCCAGTGGAT
NM_004143.3         TCACTCTCTCCTTCTGCTGGTGCCCAGAGCCCTGCTATCATCGATTCGGACCCAGTGGAT
NM_001144885.1      TCACTCTCTCCTTCTGCTGGTGCCCAGAGCCCTGCTATCATCGATTCGGACCCAGTGGAT
NM_001144887.1      TCACTCTCTCCTTCTGCTGGTGCCCAGAGCCCTGCTATCATCGATTCGGACCCAGTGGAT
                    ************************************************************

HsCD00329809        GAGGAAGTGCTGATGTCGCTGGTGGTGGAACTGGGGTTGGACCGAGCCAATGAGCTTCCG
NM_001144886.1      GAGGAAGTGCTGATGTCGCTGGTGGTGGAACTGGGGTTGGACCGAGCCAATGAGCTTCCG
NM_004143.3         GAGGAAGTGCTGATGTCGCTGGTGGTGGAACTGGGGTTGGACCGAGCCAATGAGCTTCCG
NM_001144885.1      GAGGAAGTGCTGATGTCGCTGGTGGTGGAACTGGGGTTGGACCGAGCCAATGAGCTTCCG
NM_001144887.1      GAGGAAGTGCTGATGTCGCTGGTGGTGGAACTGGGGTTGGACCGAGCCAATGAGCTTCCG
                    ************************************************************

HsCD00329809        GAGCTGTGGCTGGGGCAGAATGAGTTTGACTTCACTGCGGACTTTCCATCTAGCTGCTTG
NM_001144886.1      GAGCTGTGGCTGGGGCAGAATGAGTTTGACTTCACTGCGGACTTTCCATCTAGCTGCTAA
NM_004143.3         GAGCTGTGGCTGGGGCAGAATGAGTTTGACTTCACTGCGGACTTTCCATCTAGCTGCTAA
NM_001144885.1      GAGCTGTGGCTGGGGCAGAATGAGTTTGACTTCACTGCGGACTTTCCATCTAGCTGCTAA
NM_001144887.1      GAGCTGTGGCTGGGGCAGAATGAGTTTGACTTCACTGCGGACTTTCCATCTAGCTGCTAA
                    **********************************************************:.

Pathways

NCI : Regulation of nuclear SMAD2/3 signaling
Panther : TGF-beta signaling pathway
WikiPathway : TGF beta Signaling Pathway

Protein

UniProt : Q99966
HPRD : 02146

Clone

Cloning Information : 269213
HIP Master Clone ID : 166341
Original Clone ID : FLH269213.01L

Organism


TAX_ID : 9606
Species Specific ID: 4435

Genome

Chromosome : X
Map Location : Xq13.1
Ensembl : ENSG00000125931

Reagents

Labome : MSG1-antibody