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CFDP1 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  CFDP1
Gene Name:  craniofacial development protein 1
Sequence             
Original Clone ID: FLH269313.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329817

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 900nts         Open reading frame : 1 to 900
Reference Sequence Alignment

ATGGAGGAATTCGACTCCGAAGACTTCTCTACGTCGGAGGAGGACGAGGACTACGTGCCG
TCGGGTGGAGAGTATAGTGAAGATGATGTAAATGAATTAGTGAAGGAAGATGAAGTGGAT
GGTGAAGAGCAGACACAGAAAACCCAAGGGAAAAAAAGAAAGGCCCAGAGCATTCCAGCC
AGGAAGAGAAGACAAGGTGGCCTCTCATTAGAAGAAGAGGAAGAGGAGGATGCCAATTCA
GAATCTGAGGGAAGCAGTAGTGAGGAGGAAGATGACGCTGCAGAGCAGGAAAAAGGCATT
GGATCAGAGGATGCCAGGAAAAAGAAGGAGGACGAACTCTGGGCCAGCTTCCTCAATGAT
GTGGGACCAAAATCAAAAGTGCCCCCAAGTACACAAGTTAAGAAAGGAGAGGAGACTGAA
GAGACAAGTTCAAGTAAATTGTTGGTAAAAGCAGAAGAGCTAGAGAAACCTAAAGAAACA
GAAAAAGTTAAAATCACCAAGGTGTTTGATTTTGCTGGTGAAGAAGTAAGGGTAACTAAG
GAAGTGGATGCTACATCTAAAGAGGCCAAATCCTTCTTCAAGCAGAATGAGAAAGAAAAA
CCACAGGCTAATGTTCCTTCAGCTCTGCCATCACTCCCTGCCGGGTCAGGGTTAAAAAGA
TCAAGTGGCATGAGCAGCCTTTTGGGGAAAATTGGTGCCAAGAAGCAGAAAATGAGCACC
CTTGAGAAGTCCAAACTGGACTGGGAGAGCTTCAAGGAGGAAGAGGGGATTGGTGAAGAA
CTGGCCATCCATAATCGAGGGAAAGAGGGGTACATTGAACGGAAAGCCTTCCTCGACCGA
GTGGATCACAGGCAGTTTGAAATTGAGCGAGATCTCAGGCTGAGCAAAATGAAACCTTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1033
Start on reference sequence 134
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CFDP1
Symbol Nomenclature : CFDP1
SYNONYM : BCNT; BUCENTAUR; CENP-29; CP27; SWC5; Yeti; p97
Designation : centromere protein 29|phosphoprotein (Bucentaur)
Full Nomenclature : craniofacial development protein 1
GENEID : 10428
HGNC : 1873
MIM : 608108
Vega : OTTHUMG00000137615
Target GenBank: BC000991

 

Reference Sequence Alignment

  

HsCD00329817      ATGGAGGAATTCGACTCCGAAGACTTCTCTACGTCGGAGGAGGACGAGGACTACGTGCCG
NM_006324.2       ATGGAGGAATTCGACTCCGAAGACTTCTCTACGTCGGAGGAGGACGAGGACTACGTGCCG
                  ************************************************************

HsCD00329817      TCGGGTGGAGAGTATAGTGAAGATGATGTAAATGAATTAGTGAAGGAAGATGAAGTGGAT
NM_006324.2       TCGGGTGGAGAGTATAGTGAAGATGATGTAAATGAATTAGTGAAGGAAGATGAAGTGGAT
                  ************************************************************

HsCD00329817      GGTGAAGAGCAGACACAGAAAACCCAAGGGAAAAAAAGAAAGGCCCAGAGCATTCCAGCC
NM_006324.2       GGTGAAGAGCAGACACAGAAAACCCAAGGGAAAAAAAGAAAGGCCCAGAGCATTCCAGCC
                  ************************************************************

HsCD00329817      AGGAAGAGAAGACAAGGTGGCCTCTCATTAGAAGAAGAGGAAGAGGAGGATGCCAATTCA
NM_006324.2       AGGAAGAGAAGACAAGGTGGCCTCTCATTAGAAGAAGAGGAAGAGGAGGATGCCAATTCA
                  ************************************************************

HsCD00329817      GAATCTGAGGGAAGCAGTAGTGAGGAGGAAGATGACGCTGCAGAGCAGGAAAAAGGCATT
NM_006324.2       GAATCTGAGGGAAGCAGTAGTGAGGAGGAAGATGACGCTGCAGAGCAGGAAAAAGGCATT
                  ************************************************************

HsCD00329817      GGATCAGAGGATGCCAGGAAAAAGAAGGAGGACGAACTCTGGGCCAGCTTCCTCAATGAT
NM_006324.2       GGATCAGAGGATGCCAGGAAAAAGAAGGAGGACGAACTCTGGGCCAGCTTCCTCAATGAT
                  ************************************************************

HsCD00329817      GTGGGACCAAAATCAAAAGTGCCCCCAAGTACACAAGTTAAGAAAGGAGAGGAGACTGAA
NM_006324.2       GTGGGACCAAAATCAAAAGTGCCCCCAAGTACACAAGTTAAGAAAGGAGAGGAGACTGAA
                  ************************************************************

HsCD00329817      GAGACAAGTTCAAGTAAATTGTTGGTAAAAGCAGAAGAGCTAGAGAAACCTAAAGAAACA
NM_006324.2       GAGACAAGTTCAAGTAAATTGTTGGTAAAAGCAGAAGAGCTAGAGAAACCTAAAGAAACA
                  ************************************************************

HsCD00329817      GAAAAAGTTAAAATCACCAAGGTGTTTGATTTTGCTGGTGAAGAAGTAAGGGTAACTAAG
NM_006324.2       GAAAAAGTTAAAATCACCAAGGTGTTTGATTTTGCTGGTGAAGAAGTAAGGGTAACTAAG
                  ************************************************************

HsCD00329817      GAAGTGGATGCTACATCTAAAGAGGCCAAATCCTTCTTCAAGCAGAATGAGAAAGAAAAA
NM_006324.2       GAAGTGGATGCTACATCTAAAGAGGCCAAATCCTTCTTCAAGCAGAATGAGAAAGAAAAA
                  ************************************************************

HsCD00329817      CCACAGGCTAATGTTCCTTCAGCTCTGCCATCACTCCCTGCCGGGTCAGGGTTAAAAAGA
NM_006324.2       CCACAGGCTAATGTTCCTTCAGCTCTGCCATCACTCCCTGCCGGGTCAGGGTTAAAAAGA
                  ************************************************************

HsCD00329817      TCAAGTGGCATGAGCAGCCTTTTGGGGAAAATTGGTGCCAAGAAGCAGAAAATGAGCACC
NM_006324.2       TCAAGTGGCATGAGCAGCCTTTTGGGGAAAATTGGTGCCAAGAAGCAGAAAATGAGCACC
                  ************************************************************

HsCD00329817      CTTGAGAAGTCCAAACTGGACTGGGAGAGCTTCAAGGAGGAAGAGGGGATTGGTGAAGAA
NM_006324.2       CTTGAGAAGTCCAAACTGGACTGGGAGAGCTTCAAGGAGGAAGAGGGGATTGGTGAAGAA
                  ************************************************************

HsCD00329817      CTGGCCATCCATAATCGAGGGAAAGAGGGGTACATTGAACGGAAAGCCTTCCTCGACCGA
NM_006324.2       CTGGCCATCCATAATCGAGGGAAAGAGGGGTACATTGAACGGAAAGCCTTCCTTGACCGA
                  ***************************************************** ******

HsCD00329817      GTGGATCACAGGCAGTTTGAAATTGAGCGAGATCTCAGGCTGAGCAAAATGAAACCTTTG
NM_006324.2       GTGGATCACAGGCAGTTTGAAATTGAGCGAGATCTCAGGCTGAGCAAAATGAAACCTTGA
                  ********************************************************** .

Protein

UniProt : Q9UEE9
HPRD : 16282

Clone

Cloning Information : 269313
HIP Master Clone ID : 141834
Original Clone ID : FLH269313.01L

Organism


TAX_ID : 9606
Species Specific ID: 10428

Genome

Chromosome : 16
Map Location : 16q22.2-q22.3
Ensembl : ENSG00000153774

Reagents

Labome : CFDP1-antibody