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GIPC1 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  GIPC1
Gene Name:  GIPC PDZ domain containing family, member 1
Sequence             
Original Clone ID: FLH269316.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329820

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1002nts         Open reading frame : 1 to 1002
Reference Sequence Alignment

ATGCCGCTGGGACTGGGGCGGCGGAAAAAGGCGCCCCCTCTAGTGGAAAATGAGGAGGCT
GAGCCAGGCCGTGGAGGGCTGGGCGTGGGGGAGCCAGGGCCTCTGGGCGGAGGTGGGTCG
GGGGGCCCCCAAATGGGCTTGCCCCCCCCTCCCCCAGCCCTGCGGCCCCGCCTCGTGTTC
CACACCCAGCTGGCCCATGGCAGTCCCACTGGCCGCATCGAGGGCTTCACCAACGTCAAG
GAGCTGTATGGCAAGATCGCCGAGGCCTTCCGCCTGCCAACTGCCGAGGTGATGTTCTGC
ACCCTGAACACCCACAAAGTGGACATGGACAAGCTCCTGGGGGGCCAGATCGGGCTGGAG
GACTTCATCTTCGCCCACGTGAAGGGGCAGCGCAAGGAGGTGGAGGTGTTCAAGTCGGAG
GATGCACTCGGGCTCACCATCACGGACAACGGGGCTGGCTACGCCTTCATCAAGCGCATC
AAGGAGGGCAGCGTGATCGACCACATCCACCTCATCAGCGTGGGCGACATGATCGAGGCC
ATTAACGGGCAGAGCCTGCTGGGCTGCCGGCACTACGAGGTGGCCCGGCTGCTCAAGGAG
CTGCCCCGAGGCCGTACCTTCACGCTGAAGCTCACGGAGCCTCGCAAGGCCTTCGACATG
ATCAGCCAGCGTTCAGCGGGTGGCCGCCCTGGCTCTGGCCCACAACTGGGCACTGGCCGA
GGGACCCTGCGGCTCCGATCCCGGGGCCCCGCCACGGTGGAGGATCTGCCCTCTGCCTTT
GAAGAGAAGGCCATTGAGAAGGTGGATGACCTGCTGGAGAGTTACATGGGTATCAGGGAC
ACGGAGCTGGCGGCCACCATGGTGGAGCTGGGAAAGGACAAAAGGAACCCGGATGAGCTG
GCCGAGGCCCTGGACGAACGGCTGGGTGACTTTGCCTTCCCTGACGAGTTCGTCTTTGAC
GTCTGGGGCGCCATTGGGGACGCCAAGGTCGGCCGCTACTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1270
Start on reference sequence 269
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : GIPC1
Symbol Nomenclature : GIPC1
SYNONYM : C19orf3; GIPC; GLUT1CBP; Hs.6454; IIP-1; NIP; RGS19IP1; SEMCAP; SYNECTIIN; SYNECTIN; TIP-2
Designation : GAIP C-terminus-interacting protein|GLUT1 C-terminal binding protein|IGF-1 receptor interacting protein 1|PDZ domain-containing protein GIPC1|RGS-GAIP-interacting protein|RGS19-interacting protein 1|regulator of G-protein signalling 19 interacting protein 1|tax interaction protein 2
Full Nomenclature : GIPC PDZ domain containing family, member 1
GENEID : 10755
HGNC : 1226
MIM : 605072
Vega : OTTHUMG00000182248
Target GenBank: BC000410

 

Reference Sequence Alignment

  

HsCD00329820      ATGCCGCTGGGACTGGGGCGGCGGAAAAAGGCGCCCCCTCTAGTGGAAAATGAGGAGGCT
NM_202468.2       ATGCCGCTGGGACTGGGGCGGCGGAAAAAGGCGCCCCCTCTAGTGGAAAATGAGGAGGCT
NM_005716.3       ATGCCGCTGGGACTGGGGCGGCGGAAAAAGGCGCCCCCTCTAGTGGAAAATGAGGAGGCT
NM_202470.2       ATGCCGCTGGGACTGGGGCGGCGGAAAAAGGCGCCCCCTCTAGTGGAAAATGAGGAGGCT
                  ************************************************************

HsCD00329820      GAGCCAGGCCGTGGAGGGCTGGGCGTGGGGGAGCCAGGGCCTCTGGGCGGAGGTGGGTCG
NM_202468.2       GAGCCAGGCCGTGGAGGGCTGGGCGTGGGGGAGCCAGGGCCTCTGGGCGGAGGTGGGTCG
NM_005716.3       GAGCCAGGCCGTGGAGGGCTGGGCGTGGGGGAGCCAGGGCCTCTGGGCGGAGGTGGGTCG
NM_202470.2       GAGCCAGGCCGTGGAGGGCTGGGCGTGGGGGAGCCAGGGCCTCTGGGCGGAGGTGGGTCG
                  ************************************************************

HsCD00329820      GGGGGCCCCCAAATGGGCTTGCCCCCCCCTCCCCCAGCCCTGCGGCCCCGCCTCGTGTTC
NM_202468.2       GGGGGCCCCCAAATGGGCTTGCCCCCCCCTCCCCCAGCCCTGCGGCCCCGCCTCGTGTTC
NM_005716.3       GGGGGCCCCCAAATGGGCTTGCCCCCCCCTCCCCCAGCCCTGCGGCCCCGCCTCGTGTTC
NM_202470.2       GGGGGCCCCCAAATGGGCTTGCCCCCCCCTCCCCCAGCCCTGCGGCCCCGCCTCGTGTTC
                  ************************************************************

HsCD00329820      CACACCCAGCTGGCCCATGGCAGTCCCACTGGCCGCATCGAGGGCTTCACCAACGTCAAG
NM_202468.2       CACACCCAGCTGGCCCATGGCAGTCCCACTGGCCGCATCGAGGGCTTCACCAACGTCAAG
NM_005716.3       CACACCCAGCTGGCCCATGGCAGTCCCACTGGCCGCATCGAGGGCTTCACCAACGTCAAG
NM_202470.2       CACACCCAGCTGGCCCATGGCAGTCCCACTGGCCGCATCGAGGGCTTCACCAACGTCAAG
                  ************************************************************

HsCD00329820      GAGCTGTATGGCAAGATCGCCGAGGCCTTCCGCCTGCCAACTGCCGAGGTGATGTTCTGC
NM_202468.2       GAGCTGTATGGCAAGATCGCCGAGGCCTTCCGCCTGCCAACTGCCGAGGTGATGTTCTGC
NM_005716.3       GAGCTGTATGGCAAGATCGCCGAGGCCTTCCGCCTGCCAACTGCCGAGGTGATGTTCTGC
NM_202470.2       GAGCTGTATGGCAAGATCGCCGAGGCCTTCCGCCTGCCAACTGCCGAGGTGATGTTCTGC
                  ************************************************************

HsCD00329820      ACCCTGAACACCCACAAAGTGGACATGGACAAGCTCCTGGGGGGCCAGATCGGGCTGGAG
NM_202468.2       ACCCTGAACACCCACAAAGTGGACATGGACAAGCTCCTGGGGGGCCAGATCGGGCTGGAG
NM_005716.3       ACCCTGAACACCCACAAAGTGGACATGGACAAGCTCCTGGGGGGCCAGATCGGGCTGGAG
NM_202470.2       ACCCTGAACACCCACAAAGTGGACATGGACAAGCTCCTGGGGGGCCAGATCGGGCTGGAG
                  ************************************************************

HsCD00329820      GACTTCATCTTCGCCCACGTGAAGGGGCAGCGCAAGGAGGTGGAGGTGTTCAAGTCGGAG
NM_202468.2       GACTTCATCTTCGCCCACGTGAAGGGGCAGCGCAAGGAGGTGGAGGTGTTCAAGTCGGAG
NM_005716.3       GACTTCATCTTCGCCCACGTGAAGGGGCAGCGCAAGGAGGTGGAGGTGTTCAAGTCGGAG
NM_202470.2       GACTTCATCTTCGCCCACGTGAAGGGGCAGCGCAAGGAGGTGGAGGTGTTCAAGTCGGAG
                  ************************************************************

HsCD00329820      GATGCACTCGGGCTCACCATCACGGACAACGGGGCTGGCTACGCCTTCATCAAGCGCATC
NM_202468.2       GATGCACTCGGGCTCACCATCACGGACAACGGGGCTGGCTACGCCTTCATCAAGCGCATC
NM_005716.3       GATGCACTCGGGCTCACCATCACGGACAACGGGGCTGGCTACGCCTTCATCAAGCGCATC
NM_202470.2       GATGCACTCGGGCTCACCATCACGGACAACGGGGCTGGCTACGCCTTCATCAAGCGCATC
                  ************************************************************

HsCD00329820      AAGGAGGGCAGCGTGATCGACCACATCCACCTCATCAGCGTGGGCGACATGATCGAGGCC
NM_202468.2       AAGGAGGGCAGCGTGATCGACCACATCCACCTCATCAGCGTGGGCGACATGATCGAGGCC
NM_005716.3       AAGGAGGGCAGCGTGATCGACCACATCCACCTCATCAGCGTGGGCGACATGATCGAGGCC
NM_202470.2       AAGGAGGGCAGCGTGATCGACCACATCCACCTCATCAGCGTGGGCGACATGATCGAGGCC
                  ************************************************************

HsCD00329820      ATTAACGGGCAGAGCCTGCTGGGCTGCCGGCACTACGAGGTGGCCCGGCTGCTCAAGGAG
NM_202468.2       ATTAACGGGCAGAGCCTGCTGGGCTGCCGGCACTACGAGGTGGCCCGGCTGCTCAAGGAG
NM_005716.3       ATTAACGGGCAGAGCCTGCTGGGCTGCCGGCACTACGAGGTGGCCCGGCTGCTCAAGGAG
NM_202470.2       ATTAACGGGCAGAGCCTGCTGGGCTGCCGGCACTACGAGGTGGCCCGGCTGCTCAAGGAG
                  ************************************************************

HsCD00329820      CTGCCCCGAGGCCGTACCTTCACGCTGAAGCTCACGGAGCCTCGCAAGGCCTTCGACATG
NM_202468.2       CTGCCCCGAGGCCGTACCTTCACGCTGAAGCTCACGGAGCCTCGCAAGGCCTTCGACATG
NM_005716.3       CTGCCCCGAGGCCGTACCTTCACGCTGAAGCTCACGGAGCCTCGCAAGGCCTTCGACATG
NM_202470.2       CTGCCCCGAGGCCGTACCTTCACGCTGAAGCTCACGGAGCCTCGCAAGGCCTTCGACATG
                  ************************************************************

HsCD00329820      ATCAGCCAGCGTTCAGCGGGTGGCCGCCCTGGCTCTGGCCCACAACTGGGCACTGGCCGA
NM_202468.2       ATCAGCCAGCGTTCAGCGGGTGGCCGCCCTGGCTCTGGCCCACAACTGGGCACTGGCCGA
NM_005716.3       ATCAGCCAGCGTTCAGCGGGTGGCCGCCCTGGCTCTGGCCCACAACTGGGCACTGGCCGA
NM_202470.2       ATCAGCCAGCGTTCAGCGGGTGGCCGCCCTGGCTCTGGCCCACAACTGGGCACTGGCCGA
                  ************************************************************

HsCD00329820      GGGACCCTGCGGCTCCGATCCCGGGGCCCCGCCACGGTGGAGGATCTGCCCTCTGCCTTT
NM_202468.2       GGGACCCTGCGGCTCCGATCCCGGGGCCCCGCCACGGTGGAGGATCTGCCCTCTGCCTTT
NM_005716.3       GGGACCCTGCGGCTCCGATCCCGGGGCCCCGCCACGGTGGAGGATCTGCCCTCTGCCTTT
NM_202470.2       GGGACCCTGCGGCTCCGATCCCGGGGCCCCGCCACGGTGGAGGATCTGCCCTCTGCCTTT
                  ************************************************************

HsCD00329820      GAAGAGAAGGCCATTGAGAAGGTGGATGACCTGCTGGAGAGTTACATGGGTATCAGGGAC
NM_202468.2       GAAGAGAAGGCCATTGAGAAGGTGGATGACCTGCTGGAGAGTTACATGGGTATCAGGGAC
NM_005716.3       GAAGAGAAGGCCATTGAGAAGGTGGATGACCTGCTGGAGAGTTACATGGGTATCAGGGAC
NM_202470.2       GAAGAGAAGGCCATTGAGAAGGTGGATGACCTGCTGGAGAGTTACATGGGTATCAGGGAC
                  ************************************************************

HsCD00329820      ACGGAGCTGGCGGCCACCATGGTGGAGCTGGGAAAGGACAAAAGGAACCCGGATGAGCTG
NM_202468.2       ACGGAGCTGGCGGCCACCATGGTGGAGCTGGGAAAGGACAAAAGGAACCCGGATGAGCTG
NM_005716.3       ACGGAGCTGGCGGCCACCATGGTGGAGCTGGGAAAGGACAAAAGGAACCCGGATGAGCTG
NM_202470.2       ACGGAGCTGGCGGCCACCATGGTGGAGCTGGGAAAGGACAAAAGGAACCCGGATGAGCTG
                  ************************************************************

HsCD00329820      GCCGAGGCCCTGGACGAACGGCTGGGTGACTTTGCCTTCCCTGACGAGTTCGTCTTTGAC
NM_202468.2       GCCGAGGCCCTGGACGAACGGCTGGGTGACTTTGCCTTCCCTGACGAGTTCGTCTTTGAC
NM_005716.3       GCCGAGGCCCTGGACGAACGGCTGGGTGACTTTGCCTTCCCTGACGAGTTCGTCTTTGAC
NM_202470.2       GCCGAGGCCCTGGACGAACGGCTGGGTGACTTTGCCTTCCCTGACGAGTTCGTCTTTGAC
                  ************************************************************

HsCD00329820      GTCTGGGGCGCCATTGGGGACGCCAAGGTCGGCCGCTACTTG
NM_202468.2       GTCTGGGGCGCCATTGGGGACGCCAAGGTCGGCCGCTACTAG
NM_005716.3       GTCTGGGGCGCCATTGGGGACGCCAAGGTCGGCCGCTACTAG
NM_202470.2       GTCTGGGGCGCCATTGGGGACGCCAAGGTCGGCCGCTACTAG
                  ****************************************:*

Protein

SMART domain : PDZ : Domain present in PSD-95, Dlg, and ZO-1/2.
UniProt : O14908
UniProt : A8K4I3
HPRD : 05462

Clone

Cloning Information : 269316
HIP Master Clone ID : 151320
Original Clone ID : FLH269316.01L

Organism


TAX_ID : 9606
Species Specific ID: None

Genome

Chromosome : 19
Map Location : 19p13.1
Ensembl : ENSG00000123159

Reagents

Labome : GIPC-antibody