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MGC4618 (H. sapiens ) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  TMEM175
Gene Name:  transmembrane protein 175
Sequence             
Original Clone ID: FLH269323.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329827

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1515nts         Open reading frame : 1 to 1515
Reference Sequence Alignment

ATGTCCCAGCCCCGGACCCCAGAGCAGGCACTGGATACACCGGGGGACTGCCCCCCAGGC
AGGAGAGACGAGGACGCTGGGGAGGGGATCCAGTGCTCCCAACGCATGCTCAGCTTCAGT
GACGCCCTGCTGTCCATCATCGCCACCGTCATGATCCTGCCTGTGACCCACACGGAGATC
TCCCCAGAACAGCAGTTCGACAGAAGTGTACAGAGGCTTCTGGCAACACGGATTGCCGTC
TACCTGATGACCTTTCTCATCGTGACAGTGGCCTGGGCAGCACACACAAGGTTGTTCCAA
GTTGTTGGGAAAACAGACGACACACTTGCCCTGCTCAACCTGGCCTGCATGATGACCATC
ACCTTCCTGCCTTACACGTTTTCGTTAATGGTGACCTTCCCTGATGTGCCTCTGGGCATC
TTCTTGTTCTGTGTGTGTGTGATCGCCATCGGGGTCGTGCAGGCACTGATTGTGGGGTAC
GCATTCCACTTCCCGCACCTGCTGAGCCCGCAGATCCAGCGCTCTGCCCACAGGGCTCTG
TACCGACGACACGTCCTGGGCATCGTCCTCCAAGGCCCGGCCCTGTGCTTTGCAGCGGCC
ATCTTCTCTCTCTTCTTTGTCCCCTTGTCTTACCTGCTGATGGTGACTGTCATCCTCCTC
CCCTATGTCAGCAAGGTCACCGGCTGGTGCAGAGACAGGCTCCTGGGCCACAGGGAGCCC
TCGGCTCACCCAGTGGAAGTCTTCTCGTTTGACCTCCACGAGCCACTCAGCAAGGAGCGC
GTGGAAGCCTTCAGCGACGGAGTCTACGCCATCGTGGCCACGCTTCTCATCCTGGACATC
TGCGAAGACAACGTCCCGGACCCCAAGGATGTGAAGGAGAGGTTCAGCGGCAGCCTCGTG
GCCGCCCTGAGTGCGACCGGGCCGCGCTTCCTGGCGTACTTCGGCTCCTTCGCCACAGTG
GGACTGCTGTGGTTCGCCCACCACTCACTCTTCCTGCATGTGCGCAAGGCCACGCGGGCC
ATGGGGCTGCTGAACACGCTCTCGCTGGCCTTCGTGGGTGGCCTCCCACTAGCCTACCAG
CAGACCTCGGCCTTCGCCCGGCAGCCCCGCGATGAGCTGGAGCGCGTGCGTGTCAGCTGC
ACCATCATCTTCCTGGCCAGCATCTTCCAGCTGGCCATGTGGACCACGGCGCTGCTGCAC
CAGGCGGAGACGCTGCAGCCCTCGGTGTGGTTTGGCGGCCGGGAGCATGTGCTCATGTTC
GCCAAGCTGGCGCTGTACCCCTGTGCCAGCCTGCTGGCCTTCGCCTCCACCTGCCTGCTG
AGCAGGTTCAGTGTGGGCATCTTCCACCTCATGCAGATCGCCGTGCCCTGCGCCTTCCTG
TTGCTGCGCCTGCTCGTGGGCCTGGCCCTGGCCACCCTGCGGGTCCTGCGGGGCCTCGCC
CGGCCCGAACACCCCCCGCCAGCCCCCACGGGCCAGGACGACCCACAGTCCCAGCTCCTC
CCTGCCCCCTGCTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1622
Start on reference sequence 108
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : TMEM175
Symbol Nomenclature : TMEM175
Full Nomenclature : transmembrane protein 175
GENEID : 84286
HGNC : 28709
Vega : OTTHUMG00000118996
Target GenBank: BC005158

 

Reference Sequence Alignment

  

HsCD00329827        ATGTCCCAGCCCCGGACCCCAGAGCAGGCACTGGATACACCGGGGGACTGCCCCCCAGGC
XM_005272301.1      ATGTCCCAGCCCCGGACCCCAGAGCAGGCACTGGATACACCGGGGGACTGCCCCCCAGGC
NM_032326.2         ATGTCCCAGCCCCGGACCCCAGAGCAGGCACTGGATACACCGGGGGACTGCCCCCCAGGC
                    ************************************************************

HsCD00329827        AGGAGAGACGAGGACGCTGGGGAGGGGATCCAGTGCTCCCAACGCATGCTCAGCTTCAGT
XM_005272301.1      AGGAGAGACGAGGACGCTGGGGAGGGGATCCAGTGCTCCCAACGCATGCTCAGCTTCAGT
NM_032326.2         AGGAGAGACGAGGACGCTGGGGAGGGGATCCAGTGCTCCCAACGCATGCTCAGCTTCAGT
                    ************************************************************

HsCD00329827        GACGCCCTGCTGTCCATCATCGCCACCGTCATGATCCTGCCTGTGACCCACACGGAGATC
XM_005272301.1      GACGCCCTGCTGTCCATCATCGCCACCGTCATGATCCTGCCTGTGACCCACACGGAGATC
NM_032326.2         GACGCCCTGCTGTCCATCATCGCCACCGTCATGATCCTGCCTGTGACCCACACGGAGATC
                    ************************************************************

HsCD00329827        TCCCCAGAACAGCAGTTCGACAGAAGTGTACAGAGGCTTCTGGCAACACGGATTGCCGTC
XM_005272301.1      TCCCCAGAACAGCAGTTCGACAGAAGTGTACAGAGGCTTCTGGCAACACGGATTGCCGTC
NM_032326.2         TCCCCAGAACAGCAGTTCGACAGAAGTGTACAGAGGCTTCTGGCAACACGGATTGCCGTC
                    ************************************************************

HsCD00329827        TACCTGATGACCTTTCTCATCGTGACAGTGGCCTGGGCAGCACACACAAGGTTGTTCCAA
XM_005272301.1      TACCTGATGACCTTTCTCATCGTGACAGTGGCCTGGGCAGCACACACAAGGTTGTTCCAA
NM_032326.2         TACCTGATGACCTTTCTCATCGTGACAGTGGCCTGGGCAGCACACACAAGGTTGTTCCAA
                    ************************************************************

HsCD00329827        GTTGTTGGGAAAACAGACGACACACTTGCCCTGCTCAACCTGGCCTGCATGATGACCATC
XM_005272301.1      GTTGTTGGGAAAACAGACGACACACTTGCCCTGCTCAACCTGGCCTGCATGATGACCATC
NM_032326.2         GTTGTTGGGAAAACAGACGACACACTTGCCCTGCTCAACCTGGCCTGCATGATGACCATC
                    ************************************************************

HsCD00329827        ACCTTCCTGCCTTACACGTTTTCGTTAATGGTGACCTTCCCTGATGTGCCTCTGGGCATC
XM_005272301.1      ACCTTCCTGCCTTACACG------------------------------------------
NM_032326.2         ACCTTCCTGCCTTACACGTTTTCGTTAATGGTGACCTTCCCTGATGTGCCTCTGGGCATC
                    ******************                                          

HsCD00329827        TTCTTGTTCTGTGTGTGTGTGATCGCCATCGGGGTCGTGCAGGCACTGATTGTGGGGTAC
XM_005272301.1      ------------------------------------------GCACTGATTGTGGGGTAC
NM_032326.2         TTCTTGTTCTGTGTGTGTGTGATCGCCATCGGGGTCGTGCAGGCACTGATTGTGGGGTAC
                                                              ******************

HsCD00329827        GCATTCCACTTCCCGCACCTGCTGAGCCCGCAGATCCAGCGCTCTGCCCACAGGGCTCTG
XM_005272301.1      GCATTCCACTTCCCGCACCTGCTGAGCCCGCAGATCCAGCGCTCTGCCCACAGGGCTCTG
NM_032326.2         GCATTCCACTTCCCGCACCTGCTGAGCCCGCAGATCCAGCGCTCTGCCCACAGGGCTCTG
                    ************************************************************

HsCD00329827        TACCGACGACACGTCCTGGGCATCGTCCTCCAAGGCCCGGCCCTGTGCTTTGCAGCGGCC
XM_005272301.1      TACCGACGACACGTCCTGGGCATCGTCCTCCAAGGCCCGGCCCTGTGCTTTGCAGCGGCC
NM_032326.2         TACCGACGACACGTCCTGGGCATCGTCCTCCAAGGCCCGGCCCTGTGCTTTGCAGCGGCC
                    ************************************************************

HsCD00329827        ATCTTCTCTCTCTTCTTTGTCCCCTTGTCTTACCTGCTGATGGTGACTGTCATCCTCCTC
XM_005272301.1      ATCTTCTCTCTCTTCTTTGTCCCCTTGTCTTACCTGCTGATGGTGACTGTCATCCTCCTC
NM_032326.2         ATCTTCTCTCTCTTCTTTGTCCCCTTGTCTTACCTGCTGATGGTGACTGTCATCCTCCTC
                    ************************************************************

HsCD00329827        CCCTATGTCAGCAAGGTCACCGGCTGGTGCAGAGACAGGCTCCTGGGCCACAGGGAGCCC
XM_005272301.1      CCCTATGTCAGCAAGGTCACCGGCTGGTGCAGAGACAGGCTCCTGGGCCACAGGGAGCCC
NM_032326.2         CCCTATGTCAGCAAGGTCACCGGCTGGTGCAGAGACAGGCTCCTGGGCCACAGGGAGCCC
                    ************************************************************

HsCD00329827        TCGGCTCACCCAGTGGAAGTCTTCTCGTTTGACCTCCACGAGCCACTCAGCAAGGAGCGC
XM_005272301.1      TCGGCTCACCCAGTGGAAGTCTTCTCGTTTGACCTCCACGAGCCACTCAGCAAGGAGCGC
NM_032326.2         TCGGCTCACCCAGTGGAAGTCTTCTCGTTTGACCTCCACGAGCCACTCAGCAAGGAGCGC
                    ************************************************************

HsCD00329827        GTGGAAGCCTTCAGCGACGGAGTCTACGCCATCGTGGCCACGCTTCTCATCCTGGACATC
XM_005272301.1      GTGGAAGCCTTCAGCGACGGAGTCTACGCCATCGTGGCCACGCTTCTCATCCTGGACATC
NM_032326.2         GTGGAAGCCTTCAGCGACGGAGTCTACGCCATCGTGGCCACGCTTCTCATCCTGGACATC
                    ************************************************************

HsCD00329827        TGCGAAGACAACGTCCCGGACCCCAAGGATGTGAAGGAGAGGTTCAGCGGCAGCCTCGTG
XM_005272301.1      TGCGAAGACAACGTCCCGGACCCCAAGGATGTGAAGGAGAGGTTCAGCGGCAGCCTCGTG
NM_032326.2         TGCGAAGACAACGTCCCGGACCCCAAGGATGTGAAGGAGAGGTTCAGCGGCAGCCTCGTG
                    ************************************************************

HsCD00329827        GCCGCCCTGAGTGCGACCGGGCCGCGCTTCCTGGCGTACTTCGGCTCCTTCGCCACAGTG
XM_005272301.1      GCCGCCCTGAGTGCGACCGGGCCGCGCTTCCTGGCGTACTTCGGCTCCTTCGCCACAGTG
NM_032326.2         GCCGCCCTGAGTGCGACCGGGCCGCGCTTCCTGGCGTACTTCGGCTCCTTCGCCACAGTG
                    ************************************************************

HsCD00329827        GGACTGCTGTGGTTCGCCCACCACTCACTCTTCCTGCATGTGCGCAAGGCCACGCGGGCC
XM_005272301.1      GGACTGCTGTGGTTCGCCCACCACTCACTCTTCCTGCATGTGCGCAAGGCCACGCGGGCC
NM_032326.2         GGACTGCTGTGGTTCGCCCACCACTCACTCTTCCTGCATGTGCGCAAGGCCACGCGGGCC
                    ************************************************************

HsCD00329827        ATGGGGCTGCTGAACACGCTCTCGCTGGCCTTCGTGGGTGGCCTCCCACTAGCCTACCAG
XM_005272301.1      ATGGGGCTGCTGAACACGCTCTCGCTGGCCTTCGTGGGTGGCCTCCCACTAGCCTACCAG
NM_032326.2         ATGGGGCTGCTGAACACGCTCTCGCTGGCCTTCGTGGGTGGCCTCCCACTAGCCTACCAG
                    ************************************************************

HsCD00329827        CAGACCTCGGCCTTCGCCCGGCAGCCCCGCGATGAGCTGGAGCGCGTGCGTGTCAGCTGC
XM_005272301.1      CAGACCTCGGCCTTCGCCCGGCAGCCCCGCGATGAGCTGGAGCGCGTGCGTGTCAGCTGC
NM_032326.2         CAGACCTCGGCCTTCGCCCGGCAGCCCCGCGATGAGCTGGAGCGCGTGCGTGTCAGCTGC
                    ************************************************************

HsCD00329827        ACCATCATCTTCCTGGCCAGCATCTTCCAGCTGGCCATGTGGACCACGGCGCTGCTGCAC
XM_005272301.1      ACCATCATCTTCCTGGCCAGCATCTTCCAGCTGGCCATGTGGACCACGGCGCTGCTGCAC
NM_032326.2         ACCATCATCTTCCTGGCCAGCATCTTCCAGCTGGCCATGTGGACCACGGCGCTGCTGCAC
                    ************************************************************

HsCD00329827        CAGGCGGAGACGCTGCAGCCCTCGGTGTGGTTTGGCGGCCGGGAGCATGTGCTCATGTTC
XM_005272301.1      CAGGCGGAGACGCTGCAGCCCTCGGTGTGGTTTGGCGGCCGGGAGCATGTGCTCATGTTC
NM_032326.2         CAGGCGGAGACGCTGCAGCCCTCGGTGTGGTTTGGCGGCCGGGAGCATGTGCTCATGTTC
                    ************************************************************

HsCD00329827        GCCAAGCTGGCGCTGTACCCCTGTGCCAGCCTGCTGGCCTTCGCCTCCACCTGCCTGCTG
XM_005272301.1      GCCAAGCTGGCGCTGTACCCCTGTGCCAGCCTGCTGGCCTTCGCCTCCACCTGCCTGCTG
NM_032326.2         GCCAAGCTGGCGCTGTACCCCTGTGCCAGCCTGCTGGCCTTCGCCTCCACCTGCCTGCTG
                    ************************************************************

HsCD00329827        AGCAGGTTCAGTGTGGGCATCTTCCACCTCATGCAGATCGCCGTGCCCTGCGCCTTCCTG
XM_005272301.1      AGCAGGTTCAGTGTGGGCATCTTCCACCTCATGCAGATCGCCGTGCCCTGCGCCTTCCTG
NM_032326.2         AGCAGGTTCAGTGTGGGCATCTTCCACCTCATGCAGATCGCCGTGCCCTGCGCCTTCCTG
                    ************************************************************

HsCD00329827        TTGCTGCGCCTGCTCGTGGGCCTGGCCCTGGCCACCCTGCGGGTCCTGCGGGGCCTCGCC
XM_005272301.1      TTGCTGCGCCTGCTCGTGGGCCTGGCCCTGGCCACCCTGCGGGTCCTGCGGGGCCTCGCC
NM_032326.2         TTGCTGCGCCTGCTCGTGGGCCTGGCCCTGGCCACCCTGCGGGTCCTGCGGGGCCTCGCC
                    ************************************************************

HsCD00329827        CGGCCCGAACACCCCCCGCCAGCCCCCACGGGCCAGGACGACCCACAGTCCCAGCTCCTC
XM_005272301.1      CGGCCCGAACACCCCCCGCCAGCCCCCACGGGCCAGGACGACCCACAGTCCCAGCTCCTC
NM_032326.2         CGGCCCGAACACCCCCCGCCAGCCCCCACGGGCCAGGACGACCCACAGTCCCAGCTCCTC
                    ************************************************************

HsCD00329827        CCTGCCCCCTGCTTG
XM_005272301.1      CCTGCCCCCTGCTAG
NM_032326.2         CCTGCCCCCTGCTAG
                    *************:*

Protein

UniProt : Q9BSA9
HPRD : 14648

Clone

Cloning Information : 269323
HIP Master Clone ID : 165732
Original Clone ID : FLH269323.01L

Organism


TAX_ID : 9606
Species Specific ID: 84286

Genome

Chromosome : 4
Map Location : 4p16.3
Ensembl : ENSG00000127419

Reagents

Labome : TMEM175-antibody