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TRIP6 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  TRIP6
Gene Name:  thyroid hormone receptor interactor 6
Sequence             
Original Clone ID: FLH269325.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329829

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1431nts         Open reading frame : 1 to 1431
Reference Sequence Alignment

ATGTCGGGGCCCACCTGGCTGCCCCCGAAGCAGCCGGAGCCCGCCAGAGCCCCTCAGGGG
AGGGCGATCCCCCGCGGCACCCCGGGGCCACCACCGGCCCACGGAGCAGCACTCCAGCCC
CACCCCAGGGTCAATTTTTGCCCCCTTCCATCTGAGCAGTGTTACCAGGCCCCAGGGGGA
CCGGAGGATCGGGGGCCGGCGTGGGTGGGGTCCCATGGAGTACTCCAGCACACGCAGGGG
CTCCCTGCAGACAGGGGGGGCCTTCGCCCTGGAAGCCTGGACGCCGAGATAGACTTGCTG
AGCAGCACGCTGGCCGAGCTGAATGGGGGTCGGGGTCATGCGTCACGGCGACCAGACCGA
CAGGCATATGAGCCCCCGCCACCTCCTGCCTACCGCACGGGCTCCCTGAAGCCAAATCCA
GCCTCGCCGCTCCCAGCGTCTCCCTATGGGGGCCCCACTCCAGCCTCTTACACTACCGCC
AGCACCCCGGCTGGCCCAGCCTTCCCCGTGCAAGTGAAGGTGGCACAGCCAGTGAGGGGC
TGCGGCCCACCCAGGCGGGGAGCCTCTCAGGCCTCTGGGCCCCTCCCGGGCCCCCACTTT
CCTCTCCCAGGCCGAGGTGAAGTCTGGGGGCCTGGCTATAGGAGCCAGAGAGAGCCAGGG
CCAGGGGCCAAAGAGGAAGCTGCTGGGGTCTCTGGCCCTGCAGGAAGAGGAAGAGGAGGC
GAGCACGGGCCCCAGGTGCCCCTGAGCCAGCCTCCAGAGGATGAGCTGGATAGGCTGACG
AAGAAGCTGGTTCACGACATGAACCACCCGCCCAGCGGGGAGTACTTTGGCCAGTGTGGT
GGCTGCGGAGAAGATGTGGTTGGGGATGGGGCTGGGGTTGTGGCCCTTGATCGCGTCTTT
CACGTGGGCTGCTTTGTATGTTCTACATGCCGGGCCCAGCTTCGCGGCCAGCATTTCTAC
GCCGTGGAGAGGAGGGCATATTGCGAGGGCTGCTACGTGGCCACCCTGGAGAAATGTGCC
ACGTGCTCCCAGCCCATCCTGGACCGGATCCTGCGGGCTATGGGGAAGGCCTACCACCCT
GGCTGCTTCACCTGCGTGGTGTGTCACCGCGGCCTCGACGGCATCCCCTTCACAGTGGAT
GCTACGAGCCAGATCCACTGCATTGAGGACTTTCACAGGAAGTTTGCCCCAAGATGCTCA
GTGTGCGGTGGGGCCATAATGCCTGAGCCAGGTCAGGAGGAGACTGTGAGAATTGTTGCT
CTGGATCGAAGTTTTCACATTGGCTGTTACAAGTGCGAGGAGTGTGGGCTGCTGCTCTCC
TCTGAGGGCGAGTGTCAGGGCTGCTACCCGCTGGATGGGCACATCTTGTGCAAGGCCTGC
AGCGCCTGGCGCATCCAGGAGCTCTCAGCCACCGTCACCACTGACTGCTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1500
Start on reference sequence 70
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : TRIP6
Symbol Nomenclature : TRIP6
SYNONYM : OIP-1; OIP1; TRIP-6; TRIP6i2; ZRP-1
Designation : OPA-interacting protein 1|TR-interacting protein 6|thyroid hormone receptor interacting protein 6|thyroid receptor-interacting protein 6|zyxin related protein 1
Full Nomenclature : thyroid hormone receptor interactor 6
GENEID : 7205
Locus Tag : tcag7.1300
HGNC : 12311
MIM : 602933
Vega : OTTHUMG00000157029
Target GenBank: BC004999

 

Reference Sequence Alignment

  

HsCD00329829      ATGTCGGGGCCCACCTGGCTGCCCCCGAAGCAGCCGGAGCCCGCCAGAGCCCCTCAGGGG
NM_003302.2       ATGTCGGGGCCCACCTGGCTGCCCCCGAAGCAGCCGGAGCCCGCCAGAGCCCCTCAGGGG
                  ************************************************************

HsCD00329829      AGGGCGATCCCCCGCGGCACCCCGGGGCCACCACCGGCCCACGGAGCAGCACTCCAGCCC
NM_003302.2       AGGGCGATCCCCCGCGGCACCCCGGGGCCACCACCGGCCCACGGAGCAGCACTCCAGCCC
                  ************************************************************

HsCD00329829      CACCCCAGGGTCAATTTTTGCCCCCTTCCATCTGAGCAGTGTTACCAGGCCCCAGGGGGA
NM_003302.2       CACCCCAGGGTCAATTTTTGCCCCCTTCCATCTGAGCAGTGTTACCAGGCCCCAGGGGGA
                  ************************************************************

HsCD00329829      CCGGAGGATCGGGGGCCGGCGTGGGTGGGGTCCCATGGAGTACTCCAGCACACGCAGGGG
NM_003302.2       CCGGAGGATCGGGGGCCGGCGTGGGTGGGGTCCCATGGAGTACTCCAGCACACGCAGGGG
                  ************************************************************

HsCD00329829      CTCCCTGCAGACAGGGGGGGCCTTCGCCCTGGAAGCCTGGACGCCGAGATAGACTTGCTG
NM_003302.2       CTCCCTGCAGACAGGGGGGGCCTTCGCCCTGGAAGCCTGGACGCCGAGATAGACTTGCTG
                  ************************************************************

HsCD00329829      AGCAGCACGCTGGCCGAGCTGAATGGGGGTCGGGGTCATGCGTCACGGCGACCAGACCGA
NM_003302.2       AGCAGCACGCTGGCCGAGCTGAATGGGGGTCGGGGTCATGCGTCACGGCGACCAGACCGA
                  ************************************************************

HsCD00329829      CAGGCATATGAGCCCCCGCCACCTCCTGCCTACCGCACGGGCTCCCTGAAGCCAAATCCA
NM_003302.2       CAGGCATATGAGCCCCCGCCACCTCCTGCCTACCGCACGGGCTCCCTGAAGCCAAATCCA
                  ************************************************************

HsCD00329829      GCCTCGCCGCTCCCAGCGTCTCCCTATGGGGGCCCCACTCCAGCCTCTTACACTACCGCC
NM_003302.2       GCCTCGCCGCTCCCAGCGTCTCCCTATGGGGGCCCCACTCCAGCCTCTTACACTACCGCC
                  ************************************************************

HsCD00329829      AGCACCCCGGCTGGCCCAGCCTTCCCCGTGCAAGTGAAGGTGGCACAGCCAGTGAGGGGC
NM_003302.2       AGCACCCCGGCTGGCCCAGCCTTCCCCGTGCAAGTGAAGGTGGCACAGCCAGTGAGGGGC
                  ************************************************************

HsCD00329829      TGCGGCCCACCCAGGCGGGGAGCCTCTCAGGCCTCTGGGCCCCTCCCGGGCCCCCACTTT
NM_003302.2       TGCGGCCCACCCAGGCGGGGAGCCTCTCAGGCCTCTGGGCCCCTCCCGGGCCCCCACTTT
                  ************************************************************

HsCD00329829      CCTCTCCCAGGCCGAGGTGAAGTCTGGGGGCCTGGCTATAGGAGCCAGAGAGAGCCAGGG
NM_003302.2       CCTCTCCCAGGCCGAGGTGAAGTCTGGGGGCCTGGCTATAGGAGCCAGAGAGAGCCAGGG
                  ************************************************************

HsCD00329829      CCAGGGGCCAAAGAGGAAGCTGCTGGGGTCTCTGGCCCTGCAGGAAGAGGAAGAGGAGGC
NM_003302.2       CCAGGGGCCAAAGAGGAAGCTGCTGGGGTCTCTGGCCCTGCAGGAAGAGGAAGAGGAGGC
                  ************************************************************

HsCD00329829      GAGCACGGGCCCCAGGTGCCCCTGAGCCAGCCTCCAGAGGATGAGCTGGATAGGCTGACG
NM_003302.2       GAGCACGGGCCCCAGGTGCCCCTGAGCCAGCCTCCAGAGGATGAGCTGGATAGGCTGACG
                  ************************************************************

HsCD00329829      AAGAAGCTGGTTCACGACATGAACCACCCGCCCAGCGGGGAGTACTTTGGCCAGTGTGGT
NM_003302.2       AAGAAGCTGGTTCACGACATGAACCACCCGCCCAGCGGGGAGTACTTTGGCCAGTGTGGT
                  ************************************************************

HsCD00329829      GGCTGCGGAGAAGATGTGGTTGGGGATGGGGCTGGGGTTGTGGCCCTTGATCGCGTCTTT
NM_003302.2       GGCTGCGGAGAAGATGTGGTTGGGGATGGGGCTGGGGTTGTGGCCCTTGATCGCGTCTTT
                  ************************************************************

HsCD00329829      CACGTGGGCTGCTTTGTATGTTCTACATGCCGGGCCCAGCTTCGCGGCCAGCATTTCTAC
NM_003302.2       CACGTGGGCTGCTTTGTATGTTCTACATGCCGGGCCCAGCTTCGCGGCCAGCATTTCTAC
                  ************************************************************

HsCD00329829      GCCGTGGAGAGGAGGGCATATTGCGAGGGCTGCTACGTGGCCACCCTGGAGAAATGTGCC
NM_003302.2       GCCGTGGAGAGGAGGGCATATTGCGAGGGCTGCTACGTGGCCACCCTGGAGAAATGTGCC
                  ************************************************************

HsCD00329829      ACGTGCTCCCAGCCCATCCTGGACCGGATCCTGCGGGCTATGGGGAAGGCCTACCACCCT
NM_003302.2       ACGTGCTCCCAGCCCATCCTGGACCGGATCCTGCGGGCTATGGGGAAGGCCTACCACCCT
                  ************************************************************

HsCD00329829      GGCTGCTTCACCTGCGTGGTGTGTCACCGCGGCCTCGACGGCATCCCCTTCACAGTGGAT
NM_003302.2       GGCTGCTTCACCTGCGTGGTGTGTCACCGCGGCCTCGACGGCATCCCCTTCACAGTGGAT
                  ************************************************************

HsCD00329829      GCTACGAGCCAGATCCACTGCATTGAGGACTTTCACAGGAAGTTTGCCCCAAGATGCTCA
NM_003302.2       GCTACGAGCCAGATCCACTGCATTGAGGACTTTCACAGGAAGTTTGCCCCAAGATGCTCA
                  ************************************************************

HsCD00329829      GTGTGCGGTGGGGCCATAATGCCTGAGCCAGGTCAGGAGGAGACTGTGAGAATTGTTGCT
NM_003302.2       GTGTGCGGTGGGGCCATAATGCCTGAGCCAGGTCAGGAGGAGACTGTGAGAATTGTTGCT
                  ************************************************************

HsCD00329829      CTGGATCGAAGTTTTCACATTGGCTGTTACAAGTGCGAGGAGTGTGGGCTGCTGCTCTCC
NM_003302.2       CTGGATCGAAGTTTTCACATTGGCTGTTACAAGTGCGAGGAGTGTGGGCTGCTGCTCTCC
                  ************************************************************

HsCD00329829      TCTGAGGGCGAGTGTCAGGGCTGCTACCCGCTGGATGGGCACATCTTGTGCAAGGCCTGC
NM_003302.2       TCTGAGGGCGAGTGTCAGGGCTGCTACCCGCTGGATGGGCACATCTTGTGCAAGGCCTGC
                  ************************************************************

HsCD00329829      AGCGCCTGGCGCATCCAGGAGCTCTCAGCCACCGTCACCACTGACTGCTTG
NM_003302.2       AGCGCCTGGCGCATCCAGGAGCTCTCAGCCACCGTCACCACTGACTGCTGA
                  ************************************************* .

Pathways

NCI : AP-1 transcription factor network
NCI : LPA receptor mediated events

Clone

Cloning Information : 269325
HIP Master Clone ID : 165652
Original Clone ID : FLH269325.01L

Organism


TAX_ID : 9606
Species Specific ID: 7205

Genome

Chromosome : 7
Map Location : 7q22
Ensembl : ENSG00000087077

Reagents

Labome : TRIP6-antibody