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ID1 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  ID1
Gene Name:  inhibitor of DNA binding 1, dominant negative helix-loop-helix protein
Sequence             
Original Clone ID: FLH269326.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors:
HsCD00329830

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 468nts         Open reading frame : 1 to 468
Reference Sequence Alignment

ATGAAAGTCGCCAGTGGCAGCACCGCCACCGCCGCCGCGGGCCCCAGCTGCGCGCTGAAG
GCCGGCAAGACAGCGAGCGGTGCGGGCGAGGTGGTGCGCTGTCTGTCTGAGCAGAGCGTG
GCCATCTCGCGCTGCGCCGGGGGCGCCGGGGCGCGCCTGCCTGCCCTGCTGGACGAGCAG
CAGGTAAACGTGCTGCTCTACGACATGAACGGCTGTTACTCACGCCTCAAGGAGCTGGTG
CCCACCCTGCCCCAGAACCGCAAGGTGAGCAAGGTGGAGATTCTCCAGCACGTCATCGAC
TACATCAGGGACCTTCAGTTGGAGCTGAACTCGGAATCCGAAGTTGGGACCCCCGGGGGC
CGAGGGCTGCCGGTCCGGGCTCCGCTCAGCACCCTCAACGGCGAGATCAGCGCCCTGACG
GCCGAGGCGGCATGCGTTCCTGCGGACGATCGCATCTTGTGTCGCGAC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 554
Start on reference sequence 87
Mutations:
None
Discrepancy:
a348g
                                

5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ID1
Symbol Nomenclature : ID1
SYNONYM : ID; bHLHb24
Designation : DNA-binding protein inhibitor ID-1|class B basic helix-loop-helix protein 24|dJ857M17.1.2 (inhibitor of DNA binding 1, dominant negative helix-loop-helix protein)|inhibitor of differentiation 1
Full Nomenclature : inhibitor of DNA binding 1, dominant negative helix-loop-helix protein
GENEID : 3397
HGNC : 5360
MIM : 600349
Vega : OTTHUMG00000032181
Target GenBank: BC012420

 

Reference Sequence Alignment

  

HsCD00329830      ATGAAAGTCGCCAGTGGCAGCACCGCCACCGCCGCCGCGGGCCCCAGCTGCGCGCTGAAG
NM_002165.3       ATGAAAGTCGCCAGTGGCAGCACCGCCACCGCCGCCGCGGGCCCCAGCTGCGCGCTGAAG
NM_181353.2       ATGAAAGTCGCCAGTGGCAGCACCGCCACCGCCGCCGCGGGCCCCAGCTGCGCGCTGAAG
                  ************************************************************

HsCD00329830      GCCGGCAAGACAGCGAGCGGTGCGGGCGAGGTGGTGCGCTGTCTGTCTGAGCAGAGCGTG
NM_002165.3       GCCGGCAAGACAGCGAGCGGTGCGGGCGAGGTGGTGCGCTGTCTGTCTGAGCAGAGCGTG
NM_181353.2       GCCGGCAAGACAGCGAGCGGTGCGGGCGAGGTGGTGCGCTGTCTGTCTGAGCAGAGCGTG
                  ************************************************************

HsCD00329830      GCCATCTCGCGCTGCGCCGGGGGCGCCGGGGCGCGCCTGCCTGCCCTGCTGGACGAGCAG
NM_002165.3       GCCATCTCGCGCTGCGCCGGGGGCGCCGGGGCGCGCCTGCCTGCCCTGCTGGACGAGCAG
NM_181353.2       GCCATCTCGCGCTGCGCCGGGGGCGCCGGGGCGCGCCTGCCTGCCCTGCTGGACGAGCAG
                  ************************************************************

HsCD00329830      CAGGTAAACGTGCTGCTCTACGACATGAACGGCTGTTACTCACGCCTCAAGGAGCTGGTG
NM_002165.3       CAGGTAAACGTGCTGCTCTACGACATGAACGGCTGTTACTCACGCCTCAAGGAGCTGGTG
NM_181353.2       CAGGTAAACGTGCTGCTCTACGACATGAACGGCTGTTACTCACGCCTCAAGGAGCTGGTG
                  ************************************************************

HsCD00329830      CCCACCCTGCCCCAGAACCGCAAGGTGAGCAAGGTGGAGATTCTCCAGCACGTCATCGAC
NM_002165.3       CCCACCCTGCCCCAGAACCGCAAGGTGAGCAAGGTGGAGATTCTCCAGCACGTCATCGAC
NM_181353.2       CCCACCCTGCCCCAGAACCGCAAGGTGAGCAAGGTGGAGATTCTCCAGCACGTCATCGAC
                  ************************************************************

HsCD00329830      TACATCAGGGACCTTCAGTTGGAGCTGAACTCGGAATCCGAAGTTGGGACCCCCGGGGGC
NM_002165.3       TACATCAGGGACCTTCAGTTGGAGCTGAACTCGGAATCCGAAGTTGGAACCCCCGGGGGC
NM_181353.2       TACATCAGGGACCTTCAGTTGGAGCTGAACTCGGAATCCGAAGTTGGAACCCCCGGGGGC
                  ***********************************************.************

HsCD00329830      CGAGGGCTGCCGGTCCGGGCTCCGCTCAGCACCCTCAACGGCGAGATCAGCGCCCTGACG
NM_002165.3       CGAGGGCTGCCGGTCCGGGCTCCGCTCAGCACCCTCAACGGCGAGATCAGCGCCCTGACG
NM_181353.2       CGAGGGCTGCCGGTCCGGGCTCCGCTCAGCACCCTCAACGGCGAGATCAGCGCCCTGACG
                  ************************************************************

HsCD00329830      GCCGAGGCGGCATGCGTTCCTGCGGACGATCGCATCTTGTGTCGCGAC
NM_002165.3       GCCGAGGCGGCATGCGTTCCTGCGGACGATCGCATCTTGTGTCGCTGA
NM_181353.2       GCCGAGGTGAGATCCAGATCCGACCACTAG------------------
                  ******* *. ** *. : * *.  ** *                   

Pathways

NCI : ALK1 signaling events
NCI : IL3-mediated signaling events
NCI : Notch-mediated HES/HEY network
Reactome : Cellular Senescence
Reactome : Cellular responses to stress
Reactome : Oncogene Induced Senescence
WikiPathway : ID signaling pathway
WikiPathway : Neural Crest Differentiation

Protein

SMART domain : HLH : helix loop helix domain
UniProt : P41134
HPRD : 08980

Clone

Cloning Information : 269326
HIP Master Clone ID : 34651
Original Clone ID : FLH269326.01L

Organism


TAX_ID : 9606
Species Specific ID: 3397

Genome

Chromosome : 20
Map Location : 20q11
Ensembl : ENSG00000125968

Reagents

Labome : ID1-antibody