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NR2E1 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  NR2E1
Gene Name:  nuclear receptor subfamily 2, group E, member 1
Sequence             
Original Clone ID: FLH269331.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329835

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1158nts         Open reading frame : 1 to 1158
Reference Sequence Alignment

ATGAGCAAGCCAGCCGGATCAACAAGCCGCATTTTAGATATCCCCTGCAAAGTGTGTGGC
GACCGCAGCTCGGGGAAGCACTACGGGGTCTACGCCTGCGACGGCTGCTCAGGTTTTTTC
AAACGGAGCATCCGAAGGAATAGGACCTATGTCTGCAAATCTGGAAACCAGGGAGGCTGT
CCGGTGGACAAGACGCACAGAAACCAGTGCAGGGCGTGTCGGCTGAAGAAGTGTTTGGAA
GTCAACATGAACAAAGACGCCGTGCAGCACGAGCGGGGGCCTCGGACGTCCACCATCCGC
AAGCAAGTGGCCCTCTACTTCCGTGGACACAAGGAGGAGAACGGGGCCGCCGCGCACTTT
CCCTCGGCGGCGCTCCCTGCGCCGGCCTTCTTCACCGCGGTCACGCAGCTGGAGCCGCAC
GGCCTGGAGCTGGCCGCGGTGTCCACCACTCCAGAGCGGCAGACCCTCGTGAGCCTGGCT
CAGCCCACGCCCAAGTACCCCCATGAAGTGAATGGGACCCCAATGTATCTCTATGAAGTG
GCCACGGAGTCGGTGTGTGAATCAGCTGCCAGACTTCTCTTCATGAGCATCAAGTGGGCT
AAGAGTGTGCCAGCCTTCTCCACGCTGTCTTTGCAAGACCAGCTGATGCTTTTGGAAGAT
GCTTGGAGAGAACTGTTTGTTCTAGGAATAGCACAATGGGCCATTCCGGTTGATGCTAAC
ACTCTACTGGCTGTATCTGGCATGAACGGTGACAACACAGATTCCCAGAAGCTGAACAAG
ATCATATCTGAAATACAGGCTTTACAAGAGGTGGTGGCTCGATTTAGACAACTCCGGTTA
GATGCTACTGAATTTGCCTGTCTAAAATGCATCGTCACTTTCAAAGCCGTTCCTACACAT
AGTGGTTCTGAACTGAGAAGTTTCCGGAATGCTGCCGCCATTGCAGCCCTTCAAGATGAG
GCTCAGCTAACGCTCAACAGCTACATCCATACCAGATATCCCACTCAACCCTGTCGCTTT
GGAAAACTCCTGTTGCTTTTGCCAGCTTTACGTTCTATTAGCCCATCAACTATAGAAGAA
GTGTTTTTCAAAAAAACCATCGGCAATGTGCCAATTACAAGACTGCTTTCAGATATGTAC
AAATCCAGTGATATCTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1326
Start on reference sequence 169
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : NR2E1
Symbol Nomenclature : NR2E1
SYNONYM : TLL; TLX; XTLL
Designation : nuclear receptor TLX|nuclear receptor subfamily 2 group E member 1|protein tailless homolog|tailes-related receptor|tailless homolog
Full Nomenclature : nuclear receptor subfamily 2, group E, member 1
GENEID : 7101
Locus Tag : RP3-429G5.1
HGNC : 7973
MIM : 603849
Vega : OTTHUMG00000015319
Target GenBank: NM_003269

 

Reference Sequence Alignment

  

HsCD00329835        ------------------------------------------------------------
NM_003269.4         ------------------------------------------------------------
NM_001286102.1      ATGTTTCGAGCTGGGGCAGAGGGAGCAGAGAAGGAGCCCTCACCGCGGCCGGAATGCAGA
                                                                                

HsCD00329835        ---------------------------------------------------ATGAGCAAG
NM_003269.4         ---------------------------------------------------ATGAGCAAG
NM_001286102.1      GCGGACCCTGGCCCAGGACTGGGTTTCCCTTTAGGCTCGGGCCTACCCTGGCCCTCGCTG
                                                                       .  :  .:*

HsCD00329835        CCAGCCGGATCAACAAGCCGCATTTTAGATATCCCCTGCAAAGTGTGTGGCGACCGCAGC
NM_003269.4         CCAGCCGGATCAACAAGCCGCATTTTAGATATCCCCTGCAAAGTGTGTGGCGACCGCAGC
NM_001286102.1      TTGGAATCTCCAGGAGGCCGCATTTTAGATATCCCCTGCAAAGTGTGTGGCGACCGCAGC
                      .*..  : **. *.********************************************

HsCD00329835        TCGGGGAAGCACTACGGGGTCTACGCCTGCGACGGCTGCTCAGGTTTTTTCAAACGGAGC
NM_003269.4         TCGGGGAAGCACTACGGGGTCTACGCCTGCGACGGCTGCTCAGGTTTTTTCAAACGGAGC
NM_001286102.1      TCGGGGAAGCACTACGGGGTCTACGCCTGCGACGGCTGCTCAGGTTTTTTCAAACGGAGC
                    ************************************************************

HsCD00329835        ATCCGAAGGAATAGGACCTATGTCTGCAAATCTGGAAACCAGGGAGGCTGTCCGGTGGAC
NM_003269.4         ATCCGAAGGAATAGGACCTATGTCTGCAAATCTGGAAACCAGGGAGGCTGTCCGGTGGAC
NM_001286102.1      ATCCGAAGGAATAGGACCTATGTCTGCAAATCTGGAAACCAGGGAGGCTGTCCGGTGGAC
                    ************************************************************

HsCD00329835        AAGACGCACAGAAACCAGTGCAGGGCGTGTCGGCTGAAGAAGTGTTTGGAAGTCAACATG
NM_003269.4         AAGACGCACAGAAACCAGTGCAGGGCGTGTCGGCTGAAGAAGTGTTTGGAAGTCAACATG
NM_001286102.1      AAGACGCACAGAAACCAGTGCAGGGCGTGTCGGCTGAAGAAGTGTTTGGAAGTCAACATG
                    ************************************************************

HsCD00329835        AACAAAGACGCCGTGCAGCACGAGCGGGGGCCTCGGACGTCCACCATCCGCAAGCAAGTG
NM_003269.4         AACAAAGACGCCGTGCAGCACGAGCGGGGGCCTCGGACGTCCACCATCCGCAAGCAAGTG
NM_001286102.1      AACAAAGACGCCGTGCAGCACGAGCGGGGGCCTCGGACGTCCACCATCCGCAAGCAAGTG
                    ************************************************************

HsCD00329835        GCCCTCTACTTCCGTGGACACAAGGAGGAGAACGGGGCCGCCGCGCACTTTCCCTCGGCG
NM_003269.4         GCCCTCTACTTCCGTGGACACAAGGAGGAGAACGGGGCCGCCGCGCACTTTCCCTCGGCG
NM_001286102.1      GCCCTCTACTTCCGTGGACACAAGGAGGAGAACGGGGCCGCCGCGCACTTTCCCTCGGCG
                    ************************************************************

HsCD00329835        GCGCTCCCTGCGCCGGCCTTCTTCACCGCGGTCACGCAGCTGGAGCCGCACGGCCTGGAG
NM_003269.4         GCGCTCCCTGCGCCGGCCTTCTTCACCGCGGTCACGCAGCTGGAGCCGCACGGCCTGGAG
NM_001286102.1      GCGCTCCCTGCGCCGGCCTTCTTCACCGCGGTCACGCAGCTGGAGCCGCACGGCCTGGAG
                    ************************************************************

HsCD00329835        CTGGCCGCGGTGTCCACCACTCCAGAGCGGCAGACCCTCGTGAGCCTGGCTCAGCCCACG
NM_003269.4         CTGGCCGCGGTGTCCACCACTCCAGAGCGGCAGACCCTCGTGAGCCTGGCTCAGCCCACG
NM_001286102.1      CTGGCCGCGGTGTCCACCACTCCAGAGCGGCAGACCCTCGTGAGCCTGGCTCAGCCCACG
                    ************************************************************

HsCD00329835        CCCAAGTACCCCCATGAAGTGAATGGGACCCCAATGTATCTCTATGAAGTGGCCACGGAG
NM_003269.4         CCCAAGTACCCCCATGAAGTGAATGGGACCCCAATGTATCTCTATGAAGTGGCCACGGAG
NM_001286102.1      CCCAAGTACCCCCATGAAGTGAATGGGACCCCAATGTATCTCTATGAAGTGGCCACGGAG
                    ************************************************************

HsCD00329835        TCGGTGTGTGAATCAGCTGCCAGACTTCTCTTCATGAGCATCAAGTGGGCTAAGAGTGTG
NM_003269.4         TCGGTGTGTGAATCAGCTGCCAGACTTCTCTTCATGAGCATCAAGTGGGCTAAGAGTGTG
NM_001286102.1      TCGGTGTGTGAATCAGCTGCCAGACTTCTCTTCATGAGCATCAAGTGGGCTAAGAGTGTG
                    ************************************************************

HsCD00329835        CCAGCCTTCTCCACGCTGTCTTTGCAAGACCAGCTGATGCTTTTGGAAGATGCTTGGAGA
NM_003269.4         CCAGCCTTCTCCACGCTGTCTTTGCAAGACCAGCTGATGCTTTTGGAAGATGCTTGGAGA
NM_001286102.1      CCAGCCTTCTCCACGCTGTCTTTGCAAGACCAGCTGATGCTTTTGGAAGATGCTTGGAGA
                    ************************************************************

HsCD00329835        GAACTGTTTGTTCTAGGAATAGCACAATGGGCCATTCCGGTTGATGCTAACACTCTACTG
NM_003269.4         GAACTGTTTGTTCTAGGAATAGCACAATGGGCCATTCCGGTTGATGCTAACACTCTACTG
NM_001286102.1      GAACTGTTTGTTCTAGGAATAGCACAATGGGCCATTCCGGTTGATGCTAACACTCTACTG
                    ************************************************************

HsCD00329835        GCTGTATCTGGCATGAACGGTGACAACACAGATTCCCAGAAGCTGAACAAGATCATATCT
NM_003269.4         GCTGTATCTGGCATGAACGGTGACAACACAGATTCCCAGAAGCTGAACAAGATCATATCT
NM_001286102.1      GCTGTATCTGGCATGAACGGTGACAACACAGATTCCCAGAAGCTGAACAAGATCATATCT
                    ************************************************************

HsCD00329835        GAAATACAGGCTTTACAAGAGGTGGTGGCTCGATTTAGACAACTCCGGTTAGATGCTACT
NM_003269.4         GAAATACAGGCTTTACAAGAGGTGGTGGCTCGATTTAGACAACTCCGGTTAGATGCTACT
NM_001286102.1      GAAATACAGGCTTTACAAGAGGTGGTGGCTCGATTTAGACAACTCCGGTTAGATGCTACT
                    ************************************************************

HsCD00329835        GAATTTGCCTGTCTAAAATGCATCGTCACTTTCAAAGCCGTTCCTACACATAGTGGTTCT
NM_003269.4         GAATTTGCCTGTCTAAAATGCATCGTCACTTTCAAAGCCGTTCCTACACATAGTGGTTCT
NM_001286102.1      GAATTTGCCTGTCTAAAATGCATCGTCACTTTCAAAGCCGTTCCTACACATAGTGGTTCT
                    ************************************************************

HsCD00329835        GAACTGAGAAGTTTCCGGAATGCTGCCGCCATTGCAGCCCTTCAAGATGAGGCTCAGCTA
NM_003269.4         GAACTGAGAAGTTTCCGGAATGCTGCCGCCATTGCAGCCCTTCAAGATGAGGCTCAGCTA
NM_001286102.1      GAACTGAGAAGTTTCCGGAATGCTGCCGCCATTGCAGCCCTTCAAGATGAGGCTCAGCTA
                    ************************************************************

HsCD00329835        ACGCTCAACAGCTACATCCATACCAGATATCCCACTCAACCCTGTCGCTTTGGAAAACTC
NM_003269.4         ACGCTCAACAGCTACATCCATACCAGATATCCCACTCAACCCTGTCGCTTTGGAAAACTC
NM_001286102.1      ACGCTCAACAGCTACATCCATACCAGATATCCCACTCAACCCTGTCGCTTTGGAAAACTC
                    ************************************************************

HsCD00329835        CTGTTGCTTTTGCCAGCTTTACGTTCTATTAGCCCATCAACTATAGAAGAAGTGTTTTTC
NM_003269.4         CTGTTGCTTTTGCCAGCTTTACGTTCTATTAGCCCATCAACTATAGAAGAAGTGTTTTTC
NM_001286102.1      CTGTTGCTTTTGCCAGCTTTACGTTCTATTAGCCCATCAACTATAGAAGAAGTGTTTTTC
                    ************************************************************

HsCD00329835        AAAAAAACCATCGGCAATGTGCCAATTACAAGACTGCTTTCAGATATGTACAAATCCAGT
NM_003269.4         AAAAAAACCATCGGCAATGTGCCAATTACAAGACTGCTTTCAGATATGTACAAATCCAGT
NM_001286102.1      AAAAAAACCATCGGCAATGTGCCAATTACAAGACTGCTTTCAGATATGTACAAATCCAGT
                    ************************************************************

HsCD00329835        GATATCTTG
NM_003269.4         GATATCTAA
NM_001286102.1      GATATCTAA
                    *******:.

Pathways

Reactome : Gene Expression
Reactome : Generic Transcription Pathway
Reactome : Nuclear Receptor transcription pathway
WikiPathway : Nuclear Receptors

Protein

SMART domain : HOLI : Ligand binding domain of hormone receptors
SMART domain : ZnF_C4 : c4 zinc finger in nuclear hormone receptors
UniProt : Q9Y466
UniProt : B6ZGT9
HPRD : 07230

Clone

Cloning Information : 269331
HIP Master Clone ID : 10570
Original Clone ID : FLH269331.01L

Organism


TAX_ID : 9606
Species Specific ID: 7101

Genome

Chromosome : 6
Map Location : 6q21
Ensembl : ENSG00000112333

Reagents

Labome : NR2E1-antibody