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IFI35 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  IFI35
Gene Name:  interferon-induced protein 35
Sequence             
Original Clone ID: FLH269334.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329838

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 867nts         Open reading frame : 1 to 867
Reference Sequence Alignment

ATGTCAGCCCCACTGGATGCCGCCCTCCACGCCCTTCAGGAGGAGCAGGCCAGACTCAAG
ATGAGGCTGTGGGACCTGCAGCAGCTGAGAAAGGAGCTCGGGGACTCCCCCAAAGACAAG
GTCCCATTTTCAGTGCCCAAGATCCCCCTGGTATTCCGAGGACACACCCAGCAGGACCCG
GAAGTGCCTAAGTCTTTAGTTTCCAATTTGCGGATCCACTGCCCTCTGCTTGCGGGCTCT
GCTCTGATCACCTTTGATGACCCCAAAGTGGCTGAGCAGGTGCTGCAACAAAAGGAGCAC
ACGATCAACATGGAGGAGTGCCGGCTGCGGGTGCAGGTCCAGCCCTTGGAGCTGCCCATG
GTCACCACCATCCAGGTGATGGTGTCCAGCCAGTTGAGTGGCCGGAGGGTGTTGGTCACT
GGATTTCCTGCCAGCCTCAGGCTGAGTGAGGAGGAGCTGCTGGACAAGCTAGAGATCTTC
TTTGGCAAGACTAGGAACGGAGGTGGCGATGTGGACGTTCGGGAGCTACTGCCAGGGAGT
GTCATGCTGGGGTTTGCTAGGGATGGAGTGGCTCAGCGTCTGTGCCAAATCGGCCAGTTC
ACAGTGCCACTGGGTGGGCAGCAAGTCCCTCTGAGAGTCTCTCCGTATGTGAATGGGGAG
ATCCAGAAGGCTGAGATCAGGTCGCAGCCAGTTCCCCGCTCGGTACTGGTGCTCAACATT
CCTGATATCTTGGATGGCCCGGAGCTGCATGACGTCCTGGAGATCCACTTCCAGAAGCCC
ACCCGCGGGGGCGGGGAGGTAGAGGCCCTGACAGTCGTACCCCAAGGACAGCAGGGCCTA
GCAGTCTTCACCTCTGAGTCAGGCTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1016
Start on reference sequence 150
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : IFI35
Symbol Nomenclature : IFI35
SYNONYM : IFP35
Designation : IFP 35|ifi-35|interferon-induced 35 kDa protein
Full Nomenclature : interferon-induced protein 35
GENEID : 3430
HGNC : 5399
MIM : 600735
Vega : OTTHUMG00000167720
Target GenBank: BC001356

 

Reference Sequence Alignment

  

HsCD00329838      ATGTCAGCCCCACTGGATGCCGCCCTCCACGCCCTTCAGGAGGAGCAGGCCAGACTCAAG
NM_005533.4       ATGTCAGCCCCACTGGATGCCGCCCTCCACGCCCTTCAGGAGGAGCAGGCCAGACTCAAG
                  ************************************************************

HsCD00329838      ATGAGGCTGTGGGACCTGCAGCAGCTGAGAAAGGAGCTCGGGGACTCCCCCAAAGACAAG
NM_005533.4       ATGAGGCTGTGGGACCTGCAGCAGCTGAGAAAGGAGCTCGGGGACTCCCCCAAAGACAAG
                  ************************************************************

HsCD00329838      GTCCCATTTTCAGTGCCCAAGATCCCCCTGGTATTCCGAGGACACACCCAGCAGGACCCG
NM_005533.4       GTCCCATTTTCAGTGCCCAAGATCCCCCTGGTATTCCGAGGACACACCCAGCAGGACCCG
                  ************************************************************

HsCD00329838      GAAGTGCCTAAGTCTTTAGTTTCCAATTTGCGGATCCACTGCCCTCTGCTTGCGGGCTCT
NM_005533.4       GAAGTGCCTAAGTCTTTAGTTTCCAATTTGCGGATCCACTGCCCTCTGCTTGCGGGCTCT
                  ************************************************************

HsCD00329838      GCTCTGATCACCTTTGATGACCCCAAAGTGGCTGAGCAGGTGCTGCAACAAAAGGAGCAC
NM_005533.4       GCTCTGATCACCTTTGATGACCCCAAAGTGGCTGAGCAGGTGCTGCAACAAAAGGAGCAC
                  ************************************************************

HsCD00329838      ACGATCAACATGGAGGAGTGCCGGCTGCGGGTGCAGGTCCAGCCCTTGGAGCTGCCCATG
NM_005533.4       ACGATCAACATGGAGGAGTGCCGGCTGCGGGTGCAGGTCCAGCCCTTGGAGCTGCCCATG
                  ************************************************************

HsCD00329838      GTCACCACCATCCAGGTGATGGTGTCCAGCCAGTTGAGTGGCCGGAGGGTGTTGGTCACT
NM_005533.4       GTCACCACCATCCAGGTGATGATGTCCAGCCAGTTGAGTGGCCGGAGGGTGTTGGTCACT
                  *********************.**************************************

HsCD00329838      GGATTTCCTGCCAGCCTCAGGCTGAGTGAGGAGGAGCTGCTGGACAAGCTAGAGATCTTC
NM_005533.4       GGATTTCCTGCCAGCCTCAGGCTGAGTGAGGAGGAGCTGCTGGACAAGCTAGAGATCTTC
                  ************************************************************

HsCD00329838      TTTGGCAAGACTAGGAACGGAGGTGGCGATGTGGACGTTCGGGAGCTACTGCCAGGGAGT
NM_005533.4       TTTGGCAAGACTAGGAACGGAGGTGGCGATGTGGACGTTCGGGAGCTACTGCCAGGGAGT
                  ************************************************************

HsCD00329838      GTCATGCTGGGGTTTGCTAGGGATGGAGTGGCTCAGCGTCTGTGCCAAATCGGCCAGTTC
NM_005533.4       GTCATGCTGGGGTTTGCTAGGGATGGAGTGGCTCAGCGTCTGTGCCAAATCGGCCAGTTC
                  ************************************************************

HsCD00329838      ACAGTGCCACTGGGTGGGCAGCAAGTCCCTCTGAGAGTCTCTCCGTATGTGAATGGGGAG
NM_005533.4       ACAGTGCCACTGGGTGGGCAGCAAGTCCCTCTGAGAGTCTCTCCGTATGTGAACGGGGAG
                  ***************************************************** ******

HsCD00329838      ATCCAGAAGGCTGAGATCAGGTCGCAGCCAGTTCCCCGCTCGGTACTGGTGCTCAACATT
NM_005533.4       ATCCAGAAGGCTGAGATCAGGTCGCAGCCAGTTCCCCGCTCGGTACTGGTGCTCAACATT
                  ************************************************************

HsCD00329838      CCTGATATCTTGGATGGCCCGGAGCTGCATGACGTCCTGGAGATCCACTTCCAGAAGCCC
NM_005533.4       CCTGATATCTTGGATGGCCCGGAGCTGCATGACGTCCTGGAGATCCACTTCCAGAAGCCC
                  ************************************************************

HsCD00329838      ACCCGCGGGGGCGGGGAGGTAGAGGCCCTGACAGTCGTACCCCAAGGACAGCAGGGCCTA
NM_005533.4       ACCCGCGGGGGCGGGGAGGTAGAGGCCCTGACAGTCGTACCCCAAGGACAGCAGGGCCTA
                  ************************************************************

HsCD00329838      GCAGTCTTCACCTCTGAGTCAGGCTTG
NM_005533.4       GCAGTCTTCACCTCTGAGTCAGGCTAG
                  *************************:*

Pathways

Reactome : Cytokine Signaling in Immune system
Reactome : Immune System
Reactome : Interferon Signaling
Reactome : Interferon alpha/beta signaling

Protein

UniProt : P80217
HPRD : 02844

Clone

Cloning Information : 269334
HIP Master Clone ID : 10211
Original Clone ID : FLH269334.01L

Organism


TAX_ID : 9606
Species Specific ID: 3430

Genome

Chromosome : 17
Map Location : 17q21
Ensembl : ENSG00000068079

Reagents

Labome : IFI35-antibody