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NBL1 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  MINOS1-NBL1
Gene Name:  MINOS1-NBL1 readthrough
Sequence             
Original Clone ID: FLH269337.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329841

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 543nts         Open reading frame : 1 to 543
Reference Sequence Alignment

ATGCTTCGGGTCCTGGTGGGGGCTGTCCTCCCTGCCATGCTACTGGCTGCCCCACCACCC
ATCAACAAGCTGGCACTGTTCCCAGATAAGAGTGCCTGGTGCGAAGCCAAGAACATCACC
CAGATCGTGGGCCACAGCGGCTGTGAGGCCAAGTCCATCCAGAACAGGGCGTGCCTAGGA
CAGTGCTTCAGCTACAGCGTCCCCAACACCTTCCCACAGTCCACAGAGTCCCTGGTTCAC
TGTGACTCCTGCATGCCAGCCCAGTCCATGTGGGAGATTGTGACGCTGGAGTGCCCGGGC
CACGAGGAGGTGCCCAGGGTGGACAAGCTGGTGGAGAAGATCCTGCACTGTAGCTGCCAG
GCCTGCGGCAAGGAGCCTAGTCACGAGGGGCTGAGCGTCTATGTGCAGGGCGAGGACGGG
CCGGGATCCCAGCCCGGCACCCACCCTCACCCCCATCCCCACCCCCATCCTGGCGGGCAG
ACCCCTGAGCCCGAGGACCCCCCTGGGGCCCCCCACACAGAGGAAGAGGGGGCTGAGGAC
GAC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 691
Start on reference sequence 149
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : MINOS1-NBL1
Symbol Nomenclature : MINOS1-NBL1
SYNONYM : C1orf151-NBL1
Designation : C1orf151-NBL1 read-through protein
Full Nomenclature : MINOS1-NBL1 readthrough
GENEID : 100532736
HGNC : 48338
Vega : OTTHUMG00000183956
Target GenBank: NM_005380

 

Reference Sequence Alignment

  

HsCD00329841        ------------------------------------------------------------
NM_005380.7         ------------------------------------------------------------
NM_001204085.1      ------------------------------------------------------------
NM_001204084.2      ------------------------------------------------------------
NM_001278166.1      ------------------------------------------------------------
NM_001204089.1      ------------------------------------------------------------
NM_001278165.1      ------------------------------------------------------------
NM_001204086.2      ---ATGTGCAGCCTGGCCCTGCAGCAGCTCATTAGCATGGATTTGTTTTGGAGACTGTTT
NM_001204088.1      ------------------------------------------------------------
NM_001278164.1      ------------------------------------------------------------
NM_182744.3         ATGCCCGGCAACCTCATGAGTCAGACAAGCAGGGCTGTGTCTATCTGGAAGTTTCCAGCC
                                                                                

HsCD00329841        ------------------------------------------------ATGCTTCGGGTC
NM_005380.7         ---------------------------------------------ATGATGCTTCGGGTC
NM_001204085.1      ---------------------------------------------ATGATGCTTCGGGTC
NM_001204084.2      ---------------------------------------------ATGATGCTTCGGGTC
NM_001278166.1      ---------------------------------------------ATGATGCTTCGGGTC
NM_001204089.1      ---------------------------------------------ATGATGCTTCGGGTC
NM_001278165.1      ---------------------------------------------ATGATGCTTCGGGTC
NM_001204086.2      TTCGTTTGCGGGCAGGAGGGTCTGCGGGCTCTGGAGGCCACGGGCATGATGCTTCGGGTC
NM_001204088.1      ---ATGGTGAATGCACAGCAACTTCTGGCTCTGGAGGCCACGGGCATGATGCTTCGGGTC
NM_001278164.1      ---------------------------------------------ATGATGCTTCGGGTC
NM_182744.3         AAGCTAGGTAAAACACATGGACACAGGGCTCTGGAGGCCACGGGCATGATGCTTCGGGTC
                                                                    ************

HsCD00329841        CTGGTGGGGGCTGTCCTCCCTGCCATGCTACTGGCTGCCCCACCACCCATCAACAAGCTG
NM_005380.7         CTGGTGGGGGCTGTCCTCCCTGCCATGCTACTGGCTGCCCCACCACCCATCAACAAGCTG
NM_001204085.1      CTGGTGGGGGCTGTCCTCCCTGCCATGCTACTGGCTGCCCCACCACCCATCAACAAGCTG
NM_001204084.2      CTGGTGGGGGCTGTCCTCCCTGCCATGCTACTGGCTGCCCCACCACCCATCAACAAGCTG
NM_001278166.1      CTGGTGGGGGCTGTCCTCCCTGCCATGCTACTGGCTGCCCCACCACCCATCAACAAGCTG
NM_001204089.1      CTGGTGGGGGCTGTCCTCCCTGCCATGCTACTGGCTGCCCCACCACCCATCAACAAGCTG
NM_001278165.1      CTGGTGGGGGCTGTCCTCCCTGCCATGCTACTGGCTGCCCCACCACCCATCAACAAGCTG
NM_001204086.2      CTGGTGGGGGCTGTCCTCCCTGCCATGCTACTGGCTGCCCCACCACCCATCAACAAGCTG
NM_001204088.1      CTGGTGGGGGCTGTCCTCCCTGCCATGCTACTGGCTGCCCCACCACCCATCAACAAGCTG
NM_001278164.1      CTGGTGGGGGCTGTCCTCCCTGCCATGCTACTGGCTGCCCCACCACCCATCAACAAGCTG
NM_182744.3         CTGGTGGGGGCTGTCCTCCCTGCCATGCTACTGGCTGCCCCACCACCCATCAACAAGCTG
                    ************************************************************

HsCD00329841        GCACTGTTCCCAGATAAGAGTGCCTGGTGCGAAGCCAAGAACATCACCCAGATCGTGGGC
NM_005380.7         GCACTGTTCCCAGATAAGAGTGCCTGGTGCGAAGCCAAGAACATCACCCAGATCGTGGGC
NM_001204085.1      GCACTGTTCCCAGATAAGAGTGCCTGGTGCGAAGCCAAGAACATCACCCAGATCGTGGGC
NM_001204084.2      GCACTGTTCCCAGATAAGAGTGCCTGGTGCGAAGCCAAGAACATCACCCAGATCGTGGGC
NM_001278166.1      GCACTGTTCCCAGATAAGAGTGCCTGGTGCGAAGCCAAGAACATCACCCAGATCGTGGGC
NM_001204089.1      GCACTGTTCCCAGATAAGAGTGCCTGGTGCGAAGCCAAGAACATCACCCAGATCGTGGGC
NM_001278165.1      GCACTGTTCCCAGATAAGAGTGCCTGGTGCGAAGCCAAGAACATCACCCAGATCGTGGGC
NM_001204086.2      GCACTGTTCCCAGATAAGAGTGCCTGGTGCGAAGCCAAGAACATCACCCAGATCGTGGGC
NM_001204088.1      GCACTGTTCCCAGATAAGAGTGCCTGGTGCGAAGCCAAGAACATCACCCAGATCGTGGGC
NM_001278164.1      GCACTGTTCCCAGATAAGAGTGCCTGGTGCGAAGCCAAGAACATCACCCAGATCGTGGGC
NM_182744.3         GCACTGTTCCCAGATAAGAGTGCCTGGTGCGAAGCCAAGAACATCACCCAGATCGTGGGC
                    ************************************************************

HsCD00329841        CACAGCGGCTGTGAGGCCAAGTCCATCCAGAACAGGGCGTGCCTAGGACAGTGCTTCAGC
NM_005380.7         CACAGCGGCTGTGAGGCCAAGTCCATCCAGAACAGGGCGTGCCTAGGACAGTGCTTCAGC
NM_001204085.1      CACAGCGGCTGTGAGGCCAAGTCCATCCAGAACAGGGCGTGCCTAGGACAGTGCTTCAGC
NM_001204084.2      CACAGCGGCTGTGAGGCCAAGTCCATCCAGAACAGGGCGTGCCTAGGACAGTGCTTCAGC
NM_001278166.1      CACAGCGGCTGTGAGGCCAAGTCCATCCAGAACAGGGCGTGCCTAGGACAGTGCTTCAGC
NM_001204089.1      CACAGCGGCTGTGAGGCCAAGTCCATCCAGAACAGGGCGTGCCTAGGACAGTGCTTCAGC
NM_001278165.1      CACAGCGGCTGTGAGGCCAAGTCCATCCAGAACAGGGCGTGCCTAGGACAGTGCTTCAGC
NM_001204086.2      CACAGCGGCTGTGAGGCCAAGTCCATCCAGAACAGGGCGTGCCTAGGACAGTGCTTCAGC
NM_001204088.1      CACAGCGGCTGTGAGGCCAAGTCCATCCAGAACAGGGCGTGCCTAGGACAGTGCTTCAGC
NM_001278164.1      CACAGCGGCTGTGAGGCCAAGTCCATCCAGAACAGGGCGTGCCTAGGACAGTGCTTCAGC
NM_182744.3         CACAGCGGCTGTGAGGCCAAGTCCATCCAGAACAGGGCGTGCCTAGGACAGTGCTTCAGC
                    ************************************************************

HsCD00329841        TACAGCGTCCCCAACACCTTCCCACAGTCCACAGAGTCCCTGGTTCACTGTGACTCCTGC
NM_005380.7         TACAGCGTCCCCAACACCTTCCCACAGTCCACAGAGTCCCTGGTTCACTGTGACTCCTGC
NM_001204085.1      TACAGCGTCCCCAACACCTTCCCACAGTCCACAGAGTCCCTGGTTCACTGTGACTCCTGC
NM_001204084.2      TACAGCGTCCCCAACACCTTCCCACAGTCCACAGAGTCCCTGGTTCACTGTGACTCCTGC
NM_001278166.1      TACAGCGTCCCCAACACCTTCCCACAGTCCACAGAGTCCCTGGTTCACTGTGACTCCTGC
NM_001204089.1      TACAGCGTCCCCAACACCTTCCCACAGTCCACAGAGTCCCTGGTTCACTGTGACTCCTGC
NM_001278165.1      TACAGCGTCCCCAACACCTTCCCACAGTCCACAGAGTCCCTGGTTCACTGTGACTCCTGC
NM_001204086.2      TACAGCGTCCCCAACACCTTCCCACAGTCCACAGAGTCCCTGGTTCACTGTGACTCCTGC
NM_001204088.1      TACAGCGTCCCCAACACCTTCCCACAGTCCACAGAGTCCCTGGTTCACTGTGACTCCTGC
NM_001278164.1      TACAGCGTCCCCAACACCTTCCCACAGTCCACAGAGTCCCTGGTTCACTGTGACTCCTGC
NM_182744.3         TACAGCGTCCCCAACACCTTCCCACAGTCCACAGAGTCCCTGGTTCACTGTGACTCCTGC
                    ************************************************************

HsCD00329841        ATGCCAGCCCAGTCCATGTGGGAGATTGTGACGCTGGAGTGCCCGGGCCACGAGGAGGTG
NM_005380.7         ATGCCAGCCCAGTCCATGTGGGAGATTGTGACGCTGGAGTGCCCGGGCCACGAGGAGGTG
NM_001204085.1      ATGCCAGCCCAGTCCATGTGGGAGATTGTGACGCTGGAGTGCCCGGGCCACGAGGAGGTG
NM_001204084.2      ATGCCAGCCCAGTCCATGTGGGAGATTGTGACGCTGGAGTGCCCGGGCCACGAGGAGGTG
NM_001278166.1      ATGCCAGCCCAGTCCATGTGGGAGATTGTGACGCTGGAGTGCCCGGGCCACGAGGAGGTG
NM_001204089.1      ATGCCAGCCCAGTCCATGTGGGAGATTGTGACGCTGGAGTGCCCGGGCCACGAGGAGGTG
NM_001278165.1      ATGCCAGCCCAGTCCATGTGGGAGATTGTGACGCTGGAGTGCCCGGGCCACGAGGAGGTG
NM_001204086.2      ATGCCAGCCCAGTCCATGTGGGAGATTGTGACGCTGGAGTGCCCGGGCCACGAGGAGGTG
NM_001204088.1      ATGCCAGCCCAGTCCATGTGGGAGATTGTGACGCTGGAGTGCCCGGGCCACGAGGAGGTG
NM_001278164.1      ATGCCAGCCCAGTCCATGTGGGAGATTGTGACGCTGGAGTGCCCGGGCCACGAGGAGGTG
NM_182744.3         ATGCCAGCCCAGTCCATGTGGGAGATTGTGACGCTGGAGTGCCCGGGCCACGAGGAGGTG
                    ************************************************************

HsCD00329841        CCCAGGGTGGACAAGCTGGTGGAGAAGATCCTGCACTGTAGCTGCCAGGCCTGCGGCAAG
NM_005380.7         CCCAGGGTGGACAAGCTGGTGGAGAAGATCCTGCACTGTAGCTGCCAGGCCTGCGGCAAG
NM_001204085.1      CCCAGGGTGGACAAGCTGGTGGAGAAGATCCTGCACTGTAGCTGCCAGGCCTGCGGCAAG
NM_001204084.2      CCCAGGGTGGACAAGCTGGTGGAGAAGATCCTGCACTGTAGCTGCCAGGCCTGCGGCAAG
NM_001278166.1      CCCAGGGTGGACAAGCTGGTGGAGAAGATCCTGCACTGTAGCTGCCAGGCCTGCGGCAAG
NM_001204089.1      CCCAGGGTGGACAAGCTGGTGGAGAAGATCCTGCACTGTAGCTGCCAGGCCTGCGGCAAG
NM_001278165.1      CCCAGGGTGGACAAGCTGGTGGAGAAGATCCTGCACTGTAGCTGCCAGGCCTGCGGCAAG
NM_001204086.2      CCCAGGGTGGACAAGCTGGTGGAGAAGATCCTGCACTGTAGCTGCCAGGCCTGCGGCAAG
NM_001204088.1      CCCAGGGTGGACAAGCTGGTGGAGAAGATCCTGCACTGTAGCTGCCAGGCCTGCGGCAAG
NM_001278164.1      CCCAGGGTGGACAAGCTGGTGGAGAAGATCCTGCACTGTAGCTGCCAGGCCTGCGGCAAG
NM_182744.3         CCCAGGGTGGACAAGCTGGTGGAGAAGATCCTGCACTGTAGCTGCCAGGCCTGCGGCAAG
                    ************************************************************

HsCD00329841        GAGCCTAGTCACGAGGGGCTGAGCGTCTATGTGCAGGGCGAGGACGGGCCGGGATCCCAG
NM_005380.7         GAGCCTAGTCACGAGGGGCTGAGCGTCTATGTGCAGGGCGAGGACGGGCCGGGATCCCAG
NM_001204085.1      GAGCCTAGTCACGAGGGGCTGAGCGTCTATGTGCAGGGCGAGGACGGGCCGGGATCCCAG
NM_001204084.2      GAGCCTAGTCACGAGGGGCTGAGCGTCTATGTGCAGGGCGAGGACGGGCCGGGATCCCAG
NM_001278166.1      GAGCCTAGTCACGAGGGGCTGAGCGTCTATGTGCAGGGCGAGGACGGGCCGGGATCCCAG
NM_001204089.1      GAGCCTAGTCACGAGGGGCTGAGCGTCTATGTGCAGGGCGAGGACGGGCCGGGATCCCAG
NM_001278165.1      GAGCCTAGTCACGAGGGGCTGAGCGTCTATGTGCAGGGCGAGGACGGGCCGGGATCCCAG
NM_001204086.2      GAGCCTAGTCACGAGGGGCTGAGCGTCTATGTGCAGGGCGAGGACGGGCCGGGATCCCAG
NM_001204088.1      GAGCCTAGTCACGAGGGGCTGAGCGTCTATGTGCAGGGCGAGGACGGGCCGGGATCCCAG
NM_001278164.1      GAGCCTAGTCACGAGGGGCTGAGCGTCTATGTGCAGGGCGAGGACGGGCCGGGATCCCAG
NM_182744.3         GAGCCTAGTCACGAGGGGCTGAGCGTCTATGTGCAGGGCGAGGACGGGCCGGGATCCCAG
                    ************************************************************

HsCD00329841        CCCGGCACCCACCCTCACCCCCATCCCCACCCCCATCCTGGCGGGCAGACCCCTGAGCCC
NM_005380.7         CCCGGCACCCACCCTCACCCCCATCCCCACCCCCATCCTGGCGGGCAGACCCCTGAGCCC
NM_001204085.1      CCCGGCACCCACCCTCACCCCCATCCCCACCCCCATCCTGGCGGGCAGACCCCTGAGCCC
NM_001204084.2      CCCGGCACCCACCCTCACCCCCATCCCCACCCCCATCCTGGCGGGCAGACCCCTGAGCCC
NM_001278166.1      CCCGGCACCCACCCTCACCCCCATCCCCACCCCCATCCTGGCGGGCAGACCCCTGAGCCC
NM_001204089.1      CCCGGCACCCACCCTCACCCCCATCCCCACCCCCATCCTGGCGGGCAGACCCCTGAGCCC
NM_001278165.1      CCCGGCACCCACCCTCACCCCCATCCCCACCCCCATCCTGGCGGGCAGACCCCTGAGCCC
NM_001204086.2      CCCGGCACCCACCCTCACCCCCATCCCCACCCCCATCCTGGCGGGCAGACCCCTGAGCCC
NM_001204088.1      CCCGGCACCCACCCTCACCCCCATCCCCACCCCCATCCTGGCGGGCAGACCCCTGAGCCC
NM_001278164.1      CCCGGCACCCACCCTCACCCCCATCCCCACCCCCATCCTGGCGGGCAGACCCCTGAGCCC
NM_182744.3         CCCGGCACCCACCCTCACCCCCATCCCCACCCCCATCCTGGCGGGCAGACCCCTGAGCCC
                    ************************************************************

HsCD00329841        GAGGACCCCCCTGGGGCCCCCCACACAGAGGAAGAGGGGGCTGAGGACGAC
NM_005380.7         GAGGACCCCCCTGGGGCCCCCCACACAGAGGAAGAGGGGGCTGAGGACTGA
NM_001204085.1      GAGGACCCCCCTGGGGCCCCCCACACAGAGGAAGAGGGGGCTGAGGACTGA
NM_001204084.2      GAGGACCCCCCTGGGGCCCCCCACACAGAGGAAGAGGGGGCTGAGGACTGA
NM_001278166.1      GAGGACCCCCCTGGGGCCCCCCACACAGAGGAAGAGGGGGCTGAGGACTGA
NM_001204089.1      GAGGACCCCCCTGGGGCCCCCCACACAGAGGAAGAGGGGGCTGAGGACTGA
NM_001278165.1      GAGGACCCCCCTGGGGCCCCCCACACAGAGGAAGAGGGGGCTGAGGACTGA
NM_001204086.2      GAGGACCCCCCTGGGGCCCCCCACACAGAGGAAGAGGGGGCTGAGGACTGA
NM_001204088.1      GAGGACCCCCCTGGGGCCCCCCACACAGAGGAAGAGGGGGCTGAGGACTGA
NM_001278164.1      GAGGACCCCCCTGGGGCCCCCCACACAGAGGAAGAGGGGGCTGAGGACTGA
NM_182744.3         GAGGACCCCCCTGGGGCCCCCCACACAGAGGAAGAGGGGGCTGAGGACTGA
                    ************************************************ ..

Clone

Cloning Information : 269337
HIP Master Clone ID : 34670
Original Clone ID : FLH269337.01L

Organism


TAX_ID : 9606
Species Specific ID: 4681

Genome

Chromosome : 1
Map Location : 1p36.13
Ensembl : ENSG00000270136

Reagents

Labome : NBL1-antibody