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ELF3 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  ELF3
Gene Name:  E74-like factor 3 (ets domain transcription factor, epithelial-specific )
Sequence             
Original Clone ID: FLH269339.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329843

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1116nts         Open reading frame : 1 to 1116
Reference Sequence Alignment

ATGGCTGCAACCTGTGAGATTAGCAACATTTTTAGCAACTACTTCAGTGCGATGTACAGC
TCGGAGGACTCCACCCTGGCCTCTGTTCCCCCTGCTGCCACCTTTGGGGCCGATGACTTG
GTACTGACCCTGAGCAACCCCCAGATGTCATTGGAGGGTACAGAGAAGGCCAGCTGGTTG
GGGGAACAGCCCCAGTTCTGGTCGAAGACGCAGGTTCTGGACTGGATCAGCTACCAAGTG
GAGAAGAACAAGTACGACGCAAGCGCCATTGACTTCTCACGATGTGACATGGATGGCGCC
ACCCTCTGCAATTGTGCCCTTGAGGAGCTGCGTCTGGTCTTTGGGCCTCTGGGGGACCAA
CTCCATGCCCAGCTGCGAGACCTCACTTCCAGCTCTTCTGATGAGCTCAGTTGGATCATT
GAGCTGCTGGAGAAGGATGGCATGGCCTTCCAGGAGGCCCTAGACCCAGGGCCCTTTGAC
CAGGGCAGCCCCTTTGCCCAGGAGCTGCTGGACGACGGTCAGCAAGCCAGCCCCTACCAC
CCCGGCAGCTGTGGCGCAGGAGCCCCCTCCCCTGGCAGCTCTGACGTCTCCACCGCAGGG
ACTGGTGCTTCTCGGAGCTCCCACTCCTCAGACTCCGGTGGAAGTGACGTGGACCTGGAT
CCCACTGATGGCAAGCTCTTCCCCAGCGATGGTTTTCGTGACTGCAAGAAGGGGGATCCC
AAGCACGGGAAGCGGAAACGAGGCCGGCCCCGAAAGCTGAGCAAAGAGTACTGGGACTGT
CTCGAGGGCAAGAAGAGCAAGCACGCGCCCAGAGGCACCCACCTGTGGGAGTTCATCCGG
GACATCCTCATCCACCCGGAGCTCAACGAGGGCCTCATGAAGTGGGAGAATCGGCATGAA
GGCGTCTTCAAGTTCCTGCGCTCCGAGGCTGTGGCCCAACTATGGGGCCAAAAGAAAAAG
AACAGCAACATGACCTACGAGAAGCTGAGCCGGGCCATGAGGTACTACTACAAACGGGAG
ATCCTGGAACGGGTGGATGGCCGGCGACTCGTCTACAAGTTTGGCAAAAACTCAAGCGGC
TGGAAGGAGGAAGAGGTTCTCCAGAGTCGGAACTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1237
Start on reference sequence 122
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ELF3
Symbol Nomenclature : ELF3
SYNONYM : EPR-1; ERT; ESE-1; ESX
Designation : E74-like factor 3 (ETS domain transcription factor, serine box, epithelial-specific)|E74-like factor 3 (ets domain transcription factor)|ETS-related transcription factor Elf-3|epithelial-restricted with serine box|epithelium-restricted Ets protein ESX|epithelium-specific Ets transcription factor 1|ets domain transcription factor, serine box (epithelial-specific)
Full Nomenclature : E74-like factor 3 (ets domain transcription factor, epithelial-specific )
GENEID : 1999
Locus Tag : RP11-510N19.1
HGNC : 3318
MIM : 602191
Vega : OTTHUMG00000035867
Target GenBank: BC003569

 

Reference Sequence Alignment

  

HsCD00329843        ATGGCTGCAACCTGTGAGATTAGCAACATTTTTAGCAACTACTTCAGTGCGATGTACAGC
NM_001114309.1      ATGGCTGCAACCTGTGAGATTAGCAACATTTTTAGCAACTACTTCAGTGCGATGTACAGC
NM_004433.4         ATGGCTGCAACCTGTGAGATTAGCAACATTTTTAGCAACTACTTCAGTGCGATGTACAGC
                    ************************************************************

HsCD00329843        TCGGAGGACTCCACCCTGGCCTCTGTTCCCCCTGCTGCCACCTTTGGGGCCGATGACTTG
NM_001114309.1      TCGGAGGACTCCACCCTGGCCTCTGTTCCCCCTGCTGCCACCTTTGGGGCCGATGACTTG
NM_004433.4         TCGGAGGACTCCACCCTGGCCTCTGTTCCCCCTGCTGCCACCTTTGGGGCCGATGACTTG
                    ************************************************************

HsCD00329843        GTACTGACCCTGAGCAACCCCCAGATGTCATTGGAGGGTACAGAGAAGGCCAGCTGGTTG
NM_001114309.1      GTACTGACCCTGAGCAACCCCCAGATGTCATTGGAGGGTACAGAGAAGGCCAGCTGGTTG
NM_004433.4         GTACTGACCCTGAGCAACCCCCAGATGTCATTGGAGGGTACAGAGAAGGCCAGCTGGTTG
                    ************************************************************

HsCD00329843        GGGGAACAGCCCCAGTTCTGGTCGAAGACGCAGGTTCTGGACTGGATCAGCTACCAAGTG
NM_001114309.1      GGGGAACAGCCCCAGTTCTGGTCGAAGACGCAGGTTCTGGACTGGATCAGCTACCAAGTG
NM_004433.4         GGGGAACAGCCCCAGTTCTGGTCGAAGACGCAGGTTCTGGACTGGATCAGCTACCAAGTG
                    ************************************************************

HsCD00329843        GAGAAGAACAAGTACGACGCAAGCGCCATTGACTTCTCACGATGTGACATGGATGGCGCC
NM_001114309.1      GAGAAGAACAAGTACGACGCAAGCGCCATTGACTTCTCACGATGTGACATGGATGGCGCC
NM_004433.4         GAGAAGAACAAGTACGACGCAAGCGCCATTGACTTCTCACGATGTGACATGGATGGCGCC
                    ************************************************************

HsCD00329843        ACCCTCTGCAATTGTGCCCTTGAGGAGCTGCGTCTGGTCTTTGGGCCTCTGGGGGACCAA
NM_001114309.1      ACCCTCTGCAATTGTGCCCTTGAGGAGCTGCGTCTGGTCTTTGGGCCTCTGGGGGACCAA
NM_004433.4         ACCCTCTGCAATTGTGCCCTTGAGGAGCTGCGTCTGGTCTTTGGGCCTCTGGGGGACCAA
                    ************************************************************

HsCD00329843        CTCCATGCCCAGCTGCGAGACCTCACTTCCAGCTCTTCTGATGAGCTCAGTTGGATCATT
NM_001114309.1      CTCCATGCCCAGCTGCGAGACCTCACTTCCAGCTCTTCTGATGAGCTCAGTTGGATCATT
NM_004433.4         CTCCATGCCCAGCTGCGAGACCTCACTTCCAGCTCTTCTGATGAGCTCAGTTGGATCATT
                    ************************************************************

HsCD00329843        GAGCTGCTGGAGAAGGATGGCATGGCCTTCCAGGAGGCCCTAGACCCAGGGCCCTTTGAC
NM_001114309.1      GAGCTGCTGGAGAAGGATGGCATGGCCTTCCAGGAGGCCCTAGACCCAGGGCCCTTTGAC
NM_004433.4         GAGCTGCTGGAGAAGGATGGCATGGCCTTCCAGGAGGCCCTAGACCCAGGGCCCTTTGAC
                    ************************************************************

HsCD00329843        CAGGGCAGCCCCTTTGCCCAGGAGCTGCTGGACGACGGTCAGCAAGCCAGCCCCTACCAC
NM_001114309.1      CAGGGCAGCCCCTTTGCCCAGGAGCTGCTGGACGACGGTCAGCAAGCCAGCCCCTACCAC
NM_004433.4         CAGGGCAGCCCCTTTGCCCAGGAGCTGCTGGACGACGGTCAGCAAGCCAGCCCCTACCAC
                    ************************************************************

HsCD00329843        CCCGGCAGCTGTGGCGCAGGAGCCCCCTCCCCTGGCAGCTCTGACGTCTCCACCGCAGGG
NM_001114309.1      CCCGGCAGCTGTGGCGCAGGAGCCCCCTCCCCTGGCAGCTCTGACGTCTCCACCGCAGGG
NM_004433.4         CCCGGCAGCTGTGGCGCAGGAGCCCCCTCCCCTGGCAGCTCTGACGTCTCCACCGCAGGG
                    ************************************************************

HsCD00329843        ACTGGTGCTTCTCGGAGCTCCCACTCCTCAGACTCCGGTGGAAGTGACGTGGACCTGGAT
NM_001114309.1      ACTGGTGCTTCTCGGAGCTCCCACTCCTCAGACTCCGGTGGAAGTGACGTGGACCTGGAT
NM_004433.4         ACTGGTGCTTCTCGGAGCTCCCACTCCTCAGACTCCGGTGGAAGTGACGTGGACCTGGAT
                    ************************************************************

HsCD00329843        CCCACTGATGGCAAGCTCTTCCCCAGCGATGGTTTTCGTGACTGCAAGAAGGGGGATCCC
NM_001114309.1      CCCACTGATGGCAAGCTCTTCCCCAGCGATGGTTTTCGTGACTGCAAGAAGGGGGATCCC
NM_004433.4         CCCACTGATGGCAAGCTCTTCCCCAGCGATGGTTTTCGTGACTGCAAGAAGGGGGATCCC
                    ************************************************************

HsCD00329843        AAGCACGGGAAGCGGAAACGAGGCCGGCCCCGAAAGCTGAGCAAAGAGTACTGGGACTGT
NM_001114309.1      AAGCACGGGAAGCGGAAACGAGGCCGGCCCCGAAAGCTGAGCAAAGAGTACTGGGACTGT
NM_004433.4         AAGCACGGGAAGCGGAAACGAGGCCGGCCCCGAAAGCTGAGCAAAGAGTACTGGGACTGT
                    ************************************************************

HsCD00329843        CTCGAGGGCAAGAAGAGCAAGCACGCGCCCAGAGGCACCCACCTGTGGGAGTTCATCCGG
NM_001114309.1      CTCGAGGGCAAGAAGAGCAAGCACGCGCCCAGAGGCACCCACCTGTGGGAGTTCATCCGG
NM_004433.4         CTCGAGGGCAAGAAGAGCAAGCACGCGCCCAGAGGCACCCACCTGTGGGAGTTCATCCGG
                    ************************************************************

HsCD00329843        GACATCCTCATCCACCCGGAGCTCAACGAGGGCCTCATGAAGTGGGAGAATCGGCATGAA
NM_001114309.1      GACATCCTCATCCACCCGGAGCTCAACGAGGGCCTCATGAAGTGGGAGAATCGGCATGAA
NM_004433.4         GACATCCTCATCCACCCGGAGCTCAACGAGGGCCTCATGAAGTGGGAGAATCGGCATGAA
                    ************************************************************

HsCD00329843        GGCGTCTTCAAGTTCCTGCGCTCCGAGGCTGTGGCCCAACTATGGGGCCAAAAGAAAAAG
NM_001114309.1      GGCGTCTTCAAGTTCCTGCGCTCCGAGGCTGTGGCCCAACTATGGGGCCAAAAGAAAAAG
NM_004433.4         GGCGTCTTCAAGTTCCTGCGCTCCGAGGCTGTGGCCCAACTATGGGGCCAAAAGAAAAAG
                    ************************************************************

HsCD00329843        AACAGCAACATGACCTACGAGAAGCTGAGCCGGGCCATGAGGTACTACTACAAACGGGAG
NM_001114309.1      AACAGCAACATGACCTACGAGAAGCTGAGCCGGGCCATGAGGTACTACTACAAACGGGAG
NM_004433.4         AACAGCAACATGACCTACGAGAAGCTGAGCCGGGCCATGAGGTACTACTACAAACGGGAG
                    ************************************************************

HsCD00329843        ATCCTGGAACGGGTGGATGGCCGGCGACTCGTCTACAAGTTTGGCAAAAACTCAAGCGGC
NM_001114309.1      ATCCTGGAACGGGTGGATGGCCGGCGACTCGTCTACAAGTTTGGCAAAAACTCAAGCGGC
NM_004433.4         ATCCTGGAACGGGTGGATGGCCGGCGACTCGTCTACAAGTTTGGCAAAAACTCAAGCGGC
                    ************************************************************

HsCD00329843        TGGAAGGAGGAAGAGGTTCTCCAGAGTCGGAACTTG
NM_001114309.1      TGGAAGGAGGAAGAGGTTCTCCAGAGTCGGAACTGA
NM_004433.4         TGGAAGGAGGAAGAGGTTCTCCAGAGTCGGAACTGA
                    ********************************** .

Pathways

Panther : PDGF signaling pathway

Clone

Cloning Information : 269339
HIP Master Clone ID : 38977
Original Clone ID : FLH269339.01L

Organism


TAX_ID : 9606
Species Specific ID: 1999

Genome

Chromosome : 1
Map Location : 1q32.2
Ensembl : ENSG00000163435

Reagents

Labome : ELF3-antibody