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CRIP2 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  CRIP2
Gene Name:  cysteine-rich protein 2
Sequence             
Original Clone ID: FLH269343.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors:
HsCD00329845

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 627nts         Open reading frame : 1 to 627
Reference Sequence Alignment

ATGGCCTCCAAATGCCCCAAGTGCGACAAGACCGTGTACTTCGCCGAGAAGGTGAGCTCC
CTGGGGAAGGACTGGCACAAGTTCTGCCTCAAGTGCGAGCGCTGCAACAAGACGCTGACG
CCCGGGGGCCACGCCGAGCATGACGGGAAGCCGTTCTGCCACAAGCCGTGCTACGCCACC
CTGTTCGGACCCAAAGGCGTGAACATCGGGGGCGCGGGCTCCTACATCTACGAGAAGCCC
CTGGCGGAGGGGCCGCAGGTCACCGGCCCCATCGAGGTCCCCGCGGCCCGAGCAGAGGAG
CGGAAGGCGAGCGGCCCCCCGAAGGGGCCCAGCAGAGCCTCCAGTGTCACCACTTTCACC
GGGGAGCCCAACACGTGCCCGCGCTGCAGCAAGAAGGTGTACTTCGCTGAGAAGGTGACG
TCTCTGGGCAAGGATTGGCACCGGCCCTGCCTGCGCTGCGAGCGCTGCGGGAAGACACTG
ACCCCCGGCGGGCACGCGGAGCACGACGGCCAGCCCTACTGCCACAAGCCCTGCTATGGA
ATCCTCTTCGGACCCAAGGGAGTGAACACCGGTGCGGTGGGCAGCTACATCTATGACCGG
GACCCCGAAGGCAAGGTCCAGCCCTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 653
Start on reference sequence 27
Mutations:
None
Discrepancy:
g107a,S36N
                                

5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CRIP2
Symbol Nomenclature : CRIP2
SYNONYM : CRIP; CRP2; ESP1
Designation : Cysteine-rich intestinal protein|LIM domain protein ESP1/CRP2
Full Nomenclature : cysteine-rich protein 2
GENEID : 1397
HGNC : 2361
MIM : 601183
Vega : OTTHUMG00000029906
Target GenBank: NM_001312

 

Reference Sequence Alignment

  

HsCD00329845        ------------------------------------------------------------
NM_001312.3         ------------------------------------------------------------
NM_001270837.1      ATGCCTCCTCACCATCTTCTGCCTTGGCTGGCACAGGTCCCAAGTGCAGAGGGGGAGCTG
                                                                                

HsCD00329845        ------------------------------------------------------------
NM_001312.3         ------------------------------------------------------------
NM_001270837.1      GTCAGGCTGGTCAGTCGGGCGGGGGGGCGGGGGGCATGTTTCTGGCCTGCTGTCACCATG
                                                                                

HsCD00329845        ------------------------------------------------------------
NM_001312.3         ------------------------------------------------------------
NM_001270837.1      GAAATGGCAGTGGCTGCAGGGTGTGTGTGCAAGGGAGGAGGGTGCTGCCACAGAGAGCCC
                                                                                

HsCD00329845        ------------------------------------------ATGGCCTCCAAATGCCCC
NM_001312.3         ------------------------------------------ATGGCCTCCAAATGCCCC
NM_001270837.1      AGCCAGGACCACCATGAAAGCCAGGAGCACCGAGGGCCTCTGGTTGGCTCACAAACATGC
                                                              .* * ***..**: .  *

HsCD00329845        AAGTGCGACAAGACCGTGTACTTCGCCGAGAAGGTGAGCTCCCTGGGGAAGGACTGGCAC
NM_001312.3         AAGTGCGACAAGACCGTGTACTTCGCCGAGAAGGTGAGCTCCCTGGGGAAGGACTGGCAC
NM_001270837.1      CTGGTGCACCAGGCAGAGGGAACAGCCGAGAAGGTGAGCTCCCTGGGGAAGGACTGGCAC
                    .:*    **.**.*.*:* ..: .************************************

HsCD00329845        AAGTTCTGCCTCAAGTGCGAGCGCTGCAACAAGACGCTGACGCCCGGGGGCCACGCCGAG
NM_001312.3         AAGTTCTGCCTCAAGTGCGAGCGCTGCAGCAAGACGCTGACGCCCGGGGGCCACGCCGAG
NM_001270837.1      AAGTTCTGCCTCAAGTGCGAGCGCTGCAGCAAGACGCTGACGCCCGGGGGCCACGCCGAG
                    ****************************.*******************************

HsCD00329845        CATGACGGGAAGCCGTTCTGCCACAAGCCGTGCTACGCCACCCTGTTCGGACCCAAAGGC
NM_001312.3         CATGACGGGAAGCCGTTCTGCCACAAGCCGTGCTACGCCACCCTGTTCGGACCCAAAGGC
NM_001270837.1      CATGACGGGAAGCCGTTCTGCCACAAGCCGTGCTACGCCACCCTGTTCGGACCCAAAGGC
                    ************************************************************

HsCD00329845        GTGAACATCGGGGGCGCGGGCTCCTACATCTACGAGAAGCCCCTGGCGGAGGGGCCGCAG
NM_001312.3         GTGAACATCGGGGGCGCGGGCTCCTACATCTACGAGAAGCCCCTGGCGGAGGGGCCGCAG
NM_001270837.1      GTGAACATCGGGGGCGCGGGCTCCTACATCTACGAGAAGCCCCTGGCGGAGGGGCCGCAG
                    ************************************************************

HsCD00329845        GTCACCGGCCCCATCGAGGTCCCCGCGGCCCGAGCAGAGGAGCGGAAGGCGAGCGGCCCC
NM_001312.3         GTCACCGGCCCCATCGAGGTCCCCGCGGCCCGAGCAGAGGAGCGGAAGGCGAGCGGCCCC
NM_001270837.1      GTCACCGGCCCCATCGAGGTCCCCGCGGCCCGAGCAGAGGAGCGGAAGGCGAGCGGCCCC
                    ************************************************************

HsCD00329845        CCGAAGGGGCCCAGCAGAGCCTCCAGTGTCACCACTTTCACCGGGGAGCCCAACACGTGC
NM_001312.3         CCGAAGGGGCCCAGCAGAGCCTCCAGTGTCACCACTTTCACCGGGGAGCCCAACACGTGC
NM_001270837.1      CCGAAGGGGCCCAGCAGAGCCTCCAGTGTCACCACTTTCACCGGGGAGCCCAACACGTGC
                    ************************************************************

HsCD00329845        CCGCGCTGCAGCAAGAAGGTGTACTTCGCTGAGAAGGTGACGTCTCTGGGCAAGGATTGG
NM_001312.3         CCGCGCTGCAGCAAGAAGGTGTACTTCGCTGAGAAGGTGACGTCTCTGGGCAAGGATTGG
NM_001270837.1      CCGCGCTGCAGCAAGAAGGTGTACTTCGCTGAGAAGGTGACGTCTCTGGGCAAGGATTGG
                    ************************************************************

HsCD00329845        CACCGGCCCTGCCTGCGCTGCGAGCGCTGCGGGAAGACACTGACCCCCGGCGGGCACGCG
NM_001312.3         CACCGGCCCTGCCTGCGCTGCGAGCGCTGCGGGAAGACACTGACCCCCGGCGGGCACGCG
NM_001270837.1      CACCGGCCCTGCCTGCGCTGCGAGCGCTGCGGGAAGACACTGACCCCCGGCGGGCACGCG
                    ************************************************************

HsCD00329845        GAGCACGACGGCCAGCCCTACTGCCACAAGCCCTGCTATGGAATCCTCTTCGGACCCAAG
NM_001312.3         GAGCACGACGGCCAGCCCTACTGCCACAAGCCCTGCTATGGAATCCTCTTCGGACCCAAG
NM_001270837.1      GAGCACGACGGCCAGCCCTACTGCCACAAGCCCTGCTATGGAATCCTCTTCGGACCCAAG
                    ************************************************************

HsCD00329845        GGAGTGAACACCGGTGCGGTGGGCAGCTACATCTATGACCGGGACCCCGAAGGCAAGGTC
NM_001312.3         GGAGTGAACACCGGTGCGGTGGGCAGCTACATCTATGACCGGGACCCCGAAGGCAAGGTC
NM_001270837.1      GGAGTGAACACCGGTGCGGTGGGCAGCTACATCTATGACCGGGACCCCGAAGGCAAGGTC
                    ************************************************************

HsCD00329845        CAGCCCTTG
NM_001312.3         CAGCCCTAG
NM_001270837.1      CAGCCCTAG
                    *******:*

Clone

Cloning Information : 269343
HIP Master Clone ID : 10678
Original Clone ID : FLH269343.01L

Organism


TAX_ID : 9606
Species Specific ID: 1397

Genome

Chromosome : 14
Map Location : 14q32.3
Ensembl : ENSG00000182809

Reagents

Labome : CRIP2-antibody