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PIAS3 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  PIAS3
Gene Name:  protein inhibitor of activated STAT, 3
Sequence             
Original Clone ID: FLH269418.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329904

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1860nts         Open reading frame : 1 to 1860
Reference Sequence Alignment

ATGGTGATGAGTTTCCGGGTGTCTGAGCTCCAGGTGCTTCTTGGCTTTGCTGGCCGGAAC
AAGAGTGGACGGAAGCACGAGCTCCTGGCCAAGGCTCTGCACCTCCTGAAGTCCAGCTGT
GCCCCTAGTGTCCAGATGAAGATCAAAGAGCTTTACCGACGACGCTTTCCCCGGAAGACC
CTGGGGCCCTCTGATCTCTCCCTTCTCTCTTTGCCCCCTGGCACCTCTCCTGTAGGCTCC
CCTGGTCCTCTAGCTCCCATTCCCCCAACGCTGTTGGCCCCTGGCACCCTGCTGGGCCCC
AAGCGTGAGGTGGACATGCACCCCCCTCTGCCCCAGCCTGTGCACCCTGATGTCACCATG
AAACCATTGCCCTTCTATGAAGTCTATGGGGAGCTCATCCGGCCCACCACCCTTGCATCC
ACTTCTAGCCAGCGGTTTGAGGAAGCGCACTTTACCTTTGCCCTCACACCCCAGCAAGTG
CAGCAGATTCTTACATCCAGAGAGGTTCTGCCAGGAGCCAAATGTGATTATACCATACAG
GTGCAGCTAAGGTTCTGTCTCTGTGAGACCAGCTGCCCCCAGGAAGATTATTTTCCCCCC
AACCTCTTTGTCAAGGTCAATGGGAAACTGTGCCCCCTGCCGGGTTACCTTCCCCCAACC
AAGAATGGGGCCGAGCCCAAGAGGCCCAGCCGCCCCATCAACATCACACCCCTGGCTCGA
CTCTCAGCCACTGTTCCCAACACCATTGTGGTCAATTGGTCATCTGAGTTCGGACGGAAT
TACTCCTTGTCTGTGTACCTGGTGAGGCAGTTGACTGCAGGAACCCTTCTACAAAAACTC
AGAGCAAAGGGTATCCGGAACCCAGACCACTCGCGGGCACTGATCAAGGAGAAATTGACT
GCTGACCCTGACAGTGAGGTGGCCACTACAAGTCTCCGGGTGTCACTCATGTGCCCGCTA
GGGAAGATGCGCCTGACTGTCCCTTGTCGTGCCCTCACCTGCGCCCACCTGCAGAGCTTC
GATGCTGCCCTTTATCTACAGATGAATGAGAAGAAGCCTACATGGACATGTCCTGTGTGT
GACAAGAAGGCTCCCTATGAATCTCTTATCATTGATGGTTTATTTATGGAGATTCTTAGT
TCCTGTTCAGATTGTGATGAGATCCAATTCATGGAAGATGGATCCTGGTGCCCAATGAAA
CCCAAGAAGGAGGCATCTGAGGTTTGCCCCCCGCCAGGGTATGGGCTGGATGGCCTCCAG
TACAGCCCAGTCCAGGGGGGAGATCCATCAGAGAATAAGAAGAAGGTCGAAGTTATTGAC
TTGACAATAGAAAGCTCATCAGATGAGGAGGATCTGCCCCCTACCAAGAAGCACTGTTCT
GTCACCTCAGCTGCCATCCCGGCCCTACCTGGAAGCAAAGGAGTCCTGACATCTGGCCAC
CAGCCATCCTCGGTGCTAAGGAGCCCTGCTATGGGCACGTTGGGTGGGGATTTCCTGTCC
AGTCTCCCACTACATGAGTACCCACCTGCCTTCCCACTGGGAGCCGACATCCAAGGTTTA
GATTTATTTTCATTTCTTCAGACAGAGAGTCAGCACTATGGCCCCTCTGTCATCACCTCA
CTAGATGAACAGGATGCCCTTGGCCACTTCTTCCAGTACCGAGGGACCCCTTCTCACTTT
CTGGGCCCACTGGCCCCCACGCTGGGGAGCTCCCACTGCAGCGCCACTCCGGCGCCCCCT
CCTGGCCGTGTCAGCAGCATTGTGGCCCCTGGGGGGGCCTTGAGGGAGGGGCATGGAGGA
CCCCTGCCCTCAGGTCCCTCTTTGACTGGCTGTCGGTCAGACATCATTTCCCTGGACTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1934
Start on reference sequence 75
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : PIAS3
Symbol Nomenclature : PIAS3
SYNONYM : ZMIZ5
Designation : E3 SUMO-protein ligase PIAS3|protein inhibitor of activated STAT protein 3|zinc finger, MIZ-type containing 5
Full Nomenclature : protein inhibitor of activated STAT, 3
GENEID : 10401
HGNC : 16861
MIM : 605987
Vega : OTTHUMG00000013750
Target GenBank: BC030556

 

Reference Sequence Alignment

  

HsCD00329904      ---------------------------ATGGTGATGAGTTTCCGGGTGTCTGAGCTCCAG
NM_006099.3       ATGGCGGAGCTGGGCGAATTAAAGCACATGGTGATGAGTTTCCGGGTGTCTGAGCTCCAG
                                             *********************************

HsCD00329904      GTGCTTCTTGGCTTTGCTGGCCGGAACAAGAGTGGACGGAAGCACGAGCTCCTGGCCAAG
NM_006099.3       GTGCTTCTTGGCTTTGCTGGCCGGAACAAGAGTGGACGGAAGCACGAGCTCCTGGCCAAG
                  ************************************************************

HsCD00329904      GCTCTGCACCTCCTGAAGTCCAGCTGTGCCCCTAGTGTCCAGATGAAGATCAAAGAGCTT
NM_006099.3       GCTCTGCACCTCCTGAAGTCCAGCTGTGCCCCTAGTGTCCAGATGAAGATCAAAGAGCTT
                  ************************************************************

HsCD00329904      TACCGACGACGCTTTCCCCGGAAGACCCTGGGGCCCTCTGATCTCTCCCTTCTCTCTTTG
NM_006099.3       TACCGACGACGCTTTCCCCGGAAGACCCTGGGGCCCTCTGATCTCTCCCTTCTCTCTTTG
                  ************************************************************

HsCD00329904      CCCCCTGGCACCTCTCCTGTAGGCTCCCCTGGTCCTCTAGCTCCCATTCCCCCAACGCTG
NM_006099.3       CCCCCTGGCACCTCTCCTGTAGGCTCCCCTGGTCCTCTAGCTCCCATTCCCCCAACGCTG
                  ************************************************************

HsCD00329904      TTGGCCCCTGGCACCCTGCTGGGCCCCAAGCGTGAGGTGGACATGCACCCCCCTCTGCCC
NM_006099.3       TTGGCCCCTGGCACCCTGCTGGGCCCCAAGCGTGAGGTGGACATGCACCCCCCTCTGCCC
                  ************************************************************

HsCD00329904      CAGCCTGTGCACCCTGATGTCACCATGAAACCATTGCCCTTCTATGAAGTCTATGGGGAG
NM_006099.3       CAGCCTGTGCACCCTGATGTCACCATGAAACCATTGCCCTTCTATGAAGTCTATGGGGAG
                  ************************************************************

HsCD00329904      CTCATCCGGCCCACCACCCTTGCATCCACTTCTAGCCAGCGGTTTGAGGAAGCGCACTTT
NM_006099.3       CTCATCCGGCCCACCACCCTTGCATCCACTTCTAGCCAGCGGTTTGAGGAAGCGCACTTT
                  ************************************************************

HsCD00329904      ACCTTTGCCCTCACACCCCAGCAAGTGCAGCAGATTCTTACATCCAGAGAGGTTCTGCCA
NM_006099.3       ACCTTTGCCCTCACACCCCAGCAAGTGCAGCAGATTCTTACATCCAGAGAGGTTCTGCCA
                  ************************************************************

HsCD00329904      GGAGCCAAATGTGATTATACCATACAGGTGCAGCTAAGGTTCTGTCTCTGTGAGACCAGC
NM_006099.3       GGAGCCAAATGTGATTATACCATACAGGTGCAGCTAAGGTTCTGTCTCTGTGAGACCAGC
                  ************************************************************

HsCD00329904      TGCCCCCAGGAAGATTATTTTCCCCCCAACCTCTTTGTCAAGGTCAATGGGAAACTGTGC
NM_006099.3       TGCCCCCAGGAAGATTATTTTCCCCCCAACCTCTTTGTCAAGGTCAATGGGAAACTGTGC
                  ************************************************************

HsCD00329904      CCCCTGCCGGGTTACCTTCCCCCAACCAAGAATGGGGCCGAGCCCAAGAGGCCCAGCCGC
NM_006099.3       CCCCTGCCGGGTTACCTTCCCCCAACCAAGAATGGGGCCGAGCCCAAGAGGCCCAGCCGC
                  ************************************************************

HsCD00329904      CCCATCAACATCACACCCCTGGCTCGACTCTCAGCCACTGTTCCCAACACCATTGTGGTC
NM_006099.3       CCCATCAACATCACACCCCTGGCTCGACTCTCAGCCACTGTTCCCAACACCATTGTGGTC
                  ************************************************************

HsCD00329904      AATTGGTCATCTGAGTTCGGACGGAATTACTCCTTGTCTGTGTACCTGGTGAGGCAGTTG
NM_006099.3       AATTGGTCATCTGAGTTCGGACGGAATTACTCCTTGTCTGTGTACCTGGTGAGGCAGTTG
                  ************************************************************

HsCD00329904      ACTGCAGGAACCCTTCTACAAAAACTCAGAGCAAAGGGTATCCGGAACCCAGACCACTCG
NM_006099.3       ACTGCAGGAACCCTTCTACAAAAACTCAGAGCAAAGGGTATCCGGAACCCAGACCACTCG
                  ************************************************************

HsCD00329904      CGGGCACTGATCAAGGAGAAATTGACTGCTGACCCTGACAGTGAGGTGGCCACTACAAGT
NM_006099.3       CGGGCACTGATCAAGGAGAAATTGACTGCTGACCCTGACAGTGAGGTGGCCACTACAAGT
                  ************************************************************

HsCD00329904      CTCCGGGTGTCACTCATGTGCCCGCTAGGGAAGATGCGCCTGACTGTCCCTTGTCGTGCC
NM_006099.3       CTCCGGGTGTCACTCATGTGCCCGCTAGGGAAGATGCGCCTGACTGTCCCTTGTCGTGCC
                  ************************************************************

HsCD00329904      CTCACCTGCGCCCACCTGCAGAGCTTCGATGCTGCCCTTTATCTACAGATGAATGAGAAG
NM_006099.3       CTCACCTGCGCCCACCTGCAGAGCTTCGATGCTGCCCTTTATCTACAGATGAATGAGAAG
                  ************************************************************

HsCD00329904      AAGCCTACATGGACATGTCCTGTGTGTGACAAGAAGGCTCCCTATGAATCTCTTATCATT
NM_006099.3       AAGCCTACATGGACATGTCCTGTGTGTGACAAGAAGGCTCCCTATGAATCTCTTATCATT
                  ************************************************************

HsCD00329904      GATGGTTTATTTATGGAGATTCTTAGTTCCTGTTCAGATTGTGATGAGATCCAATTCATG
NM_006099.3       GATGGTTTATTTATGGAGATTCTTAGTTCCTGTTCAGATTGTGATGAGATCCAATTCATG
                  ************************************************************

HsCD00329904      GAAGATGGATCCTGGTGCCCAATGAAACCCAAGAAGGAGGCATCTGAGGTTTGCCCCCCG
NM_006099.3       GAAGATGGATCCTGGTGCCCAATGAAACCCAAGAAGGAGGCATCTGAGGTTTGCCCCCCG
                  ************************************************************

HsCD00329904      CCAGGGTATGGGCTGGATGGCCTCCAGTACAGCCCAGTCCAGGGGGGAGATCCATCAGAG
NM_006099.3       CCAGGGTATGGGCTGGATGGCCTCCAGTACAGCCCAGTCCAGGGGGGAGATCCATCAGAG
                  ************************************************************

HsCD00329904      AATAAGAAGAAGGTCGAAGTTATTGACTTGACAATAGAAAGCTCATCAGATGAGGAGGAT
NM_006099.3       AATAAGAAGAAGGTCGAAGTTATTGACTTGACAATAGAAAGCTCATCAGATGAGGAGGAT
                  ************************************************************

HsCD00329904      CTGCCCCCTACCAAGAAGCACTGTTCTGTCACCTCAGCTGCCATCCCGGCCCTACCTGGA
NM_006099.3       CTGCCCCCTACCAAGAAGCACTGTTCTGTCACCTCAGCTGCCATCCCGGCCCTACCTGGA
                  ************************************************************

HsCD00329904      AGCAAAGGAGTCCTGACATCTGGCCACCAGCCATCCTCGGTGCTAAGGAGCCCTGCTATG
NM_006099.3       AGCAAAGGAGTCCTGACATCTGGCCACCAGCCATCCTCGGTGCTAAGGAGCCCTGCTATG
                  ************************************************************

HsCD00329904      GGCACGTTGGGTGGGGATTTCCTGTCCAGTCTCCCACTACATGAGTACCCACCTGCCTTC
NM_006099.3       GGCACGTTGGGTGGGGATTTCCTGTCCAGTCTCCCACTACATGAGTACCCACCTGCCTTC
                  ************************************************************

HsCD00329904      CCACTGGGAGCCGACATCCAAGGTTTAGATTTATTTTCATTTCTTCAGACAGAGAGTCAG
NM_006099.3       CCACTGGGAGCCGACATCCAAGGTTTAGATTTATTTTCATTTCTTCAGACAGAGAGTCAG
                  ************************************************************

HsCD00329904      CACTATGGCCCCTCTGTCATCACCTCACTAGATGAACAGGATGCCCTTGGCCACTTCTTC
NM_006099.3       CACTATGGCCCCTCTGTCATCACCTCACTAGATGAACAGGATGCCCTTGGCCACTTCTTC
                  ************************************************************

HsCD00329904      CAGTACCGAGGGACCCCTTCTCACTTTCTGGGCCCACTGGCCCCCACGCTGGGGAGCTCC
NM_006099.3       CAGTACCGAGGGACCCCTTCTCACTTTCTGGGCCCACTGGCCCCCACGCTGGGGAGCTCC
                  ************************************************************

HsCD00329904      CACTGCAGCGCCACTCCGGCGCCCCCTCCTGGCCGTGTCAGCAGCATTGTGGCCCCTGGG
NM_006099.3       CACTGCAGCGCCACTCCGGCGCCCCCTCCTGGCCGTGTCAGCAGCATTGTGGCCCCTGGG
                  ************************************************************

HsCD00329904      GGGGCCTTGAGGGAGGGGCATGGAGGACCCCTGCCCTCAGGTCCCTCTTTGACTGGCTGT
NM_006099.3       GGGGCCTTGAGGGAGGGGCATGGAGGACCCCTGCCCTCAGGTCCCTCTTTGACTGGCTGT
                  ************************************************************

HsCD00329904      CGGTCAGACATCATTTCCCTGGACTTG
NM_006099.3       CGGTCAGACATCATTTCCCTGGACTGA
                  ************************* .

Pathways

NCI : C-MYB transcription factor network
NCI : Coregulation of Androgen receptor activity
NCI : IL6-mediated signaling events
NCI : Regulation of nuclear SMAD2/3 signaling
Panther : Interferon-gamma signaling pathway
Panther : JAK/STAT signaling pathway
WikiPathway : Androgen receptor signaling pathway
WikiPathway : EGF/EGFR Signaling Pathway
WikiPathway : Prolactin Signaling Pathway

Clone

Cloning Information : 269418
HIP Master Clone ID : 196427
Original Clone ID : FLH269418.01L

Organism


TAX_ID : 9606
Species Specific ID: 10401

Genome

Chromosome : 1
Map Location : 1q21
Ensembl : ENSG00000131788

Reagents

Labome : PIAS3-antibody