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C16orf44 (H. sapiens ) in pLenti6.2/V5-DEST

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Explanation of Terms


Gene: Gene Symbol:  KLHL36
Gene Name:  kelch-like family member 36
Sequence             
Original Clone ID: FLH268277.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00330102

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1851nts         Open reading frame : 1 to 1851
Reference Sequence Alignment

ATGATGGAGGGAAGCAGGCAGACGCGAGTGTCTCGGCCATACAAGATCAGCGAATCATCA
AAGGTATACCGCTGGGCCGACCACTCAAGCACGGTGCTGCAGCGGCTGAACGAGCAGCGT
CTCCGCGGGCTCTTCTGCGACGTCGTCCTGGTGGCCGATGAGCAGCGTGTGCCAGCCCAT
CGCAACCTGCTGGCCGTGTGCAGCGACTACTTCAACTCCATGTTCACCATCGGCATGCGG
GAAGCTTTCCAGAAGGAGGTGGAGCTGATCGGCGCCTCCTACATTGGGCTCAAGGCCGTG
GTGGACTTCCTGTACGGCGGGGAGCTGGTGCTGGATGGCGGCAACATTGACTACGTCCTG
GAGACGGCTCACCTGCTGCAGATCTGGACGGTGGTAGACTTCTGCTGTGAGTACCTGGAG
CAGGAGGTGAGCGAGGACAACTACCTGTACCTGCAGGAGCTGGCCTCCATCTACAGCCTC
AAGCGGCTTGATGCCTTCATCGATGGCTTCATCCTGAACCACTTCGGCACGCTGTCCTTT
ACGCCCGACTTCCTGCAGAACGTCTCCATGCAGAAGCTGTGTGTCTACCTGAGCAGCAGC
GAGGTGCAGCGGGAGTGTGAGCACGACCTCTTGCAGGCCGCCCTGCAGTGGCTGACGCAG
CAGCCCGAGCGCGAGGCCCACGCCCGCCAGGTGCTGGAGAACATCCACTTCCCGCTCATC
CCCAAGAACGACCTGCTGCACCGCGTCAAGCCGGCCGTGTGCTCGCTGCTGCCCAAGGAG
GCCAACTGCGAGGGCTTCATCGAGGAGGCCGTGCGCTACCACAACAACCTGGCGGCCCAG
CCCGTCATGCAGACCAAGCGCACGGCGCTGCGCACCAACCAGGAGCGCCTGCTGTTTGTG
GGCGGCGAGGTCTCCGAGCGGTGTCTGGAGCTCAGTGACGACACCTGCTACCTGGACGCC
AAGAGCGAGCAGTGGGTCAAAGAGACGCCGCTGCCCGCCCGGCGGAGCCACCACTGTGTC
GCGGTGCTGGGGGGCTTCATCTTCATCGCCGGCGGCAGCTTCTCACGGGACAACGGAGGG
GATGCGGCCTCCAATCTTCTTTATAGGTATGACCCCCGCTGTAAACAGTGGATCAAGGTG
GCCTCCATGAACCAGCGCCGTGTGGATTTCTACCTTGCCTCCATCGAAGACATGCTGGTG
GCCATCGGCGGCCGGAATGAGAACGGAGCGCTCTCTTCAGTAGAGACGTACAGTCCCAAG
ACTGACTCCTGGTCCTATGTGGCCGGCTTGCCAAGGTTCACGTACGGCCACGCGGGCACC
ATCTACAAAGACTTCGTGTACATCTCGGGGGGCCACGACTACCAAATTGGCCCCTACCGC
AAGAACCTGCTATGCTACGACCACCGGACAGACGTGTGGGAGGAGCGGCGGCCCATGACC
ACGGCGCGCGGCTGGCACAGCATGTGCAGCCTGGGTGACAGCATCTACTCCATCGGGGGC
AGCGATGACAACATCGAGTCCATGGAGCGCTTCGACGTGCTGGGCGTGGAGGCCTACAGC
CCGCAGTGCAACCAGTGGACCCGCGTGGCGCCGCTGCTGCACGCCAACAGCGAGTCGGGC
GTGGCAGTGTGGGAGGGCCGCATCTACATCCTGGGCGGCTACAGCTGGGAGAACACTGCC
TTCTCCAAGACCGTGCAGGTGTACGACCGCGAGGCCGACAAGTGGAGCAGGGGCGTCGAC
CTGCCCAAGGCCATCGCTGGCGGGTCCGCCTGTGTCTGCGCCCTGGAGCCACGGCCAGAG
GACAAGAAGAAGAAAGGCAAAGGCAAGAGGCACCAGGACCGGGGCCAGTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1992
Start on reference sequence 142
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : KLHL36
Symbol Nomenclature : KLHL36
SYNONYM : C16orf44
Designation : kelch-like 36|kelch-like protein 36
Full Nomenclature : kelch-like family member 36
GENEID : 79786
HGNC : 17844
Vega : OTTHUMG00000137642
Target GenBank: BC033821

 

Reference Sequence Alignment

  

HsCD00330102        ATGATGGAGGGAAGCAGGCAGACGCGAGTGTCTCGGCCATACAAGATCAGCGAATCATCA
NM_024731.2         ATGATGGAGGGAAGCAGGCAGACGCGAGTGTCTCGGCCATACAAGATCAGCGAATCATCA
XM_005256149.1      ATGATGGAGGGAAGCAGGCAGACGCGAGTGTCTCGGCCATACAAGATCAGCGAATCATCA
                    ************************************************************

HsCD00330102        AAGGTATACCGCTGGGCCGACCACTCAAGCACGGTGCTGCAGCGGCTGAACGAGCAGCGT
NM_024731.2         AAGGTATACCGCTGGGCCGACCACTCAAGCACGGTGCTGCAGCGGCTGAACGAGCAGCGT
XM_005256149.1      AAGGTATACCGCTGGGCCGACCACTCAAGCACGGTGCTGCAGCGGCTGAACGAGCAGCGT
                    ************************************************************

HsCD00330102        CTCCGCGGGCTCTTCTGCGACGTCGTCCTGGTGGCCGATGAGCAGCGTGTGCCAGCCCAT
NM_024731.2         CTCCGCGGGCTCTTCTGCGACGTCGTCCTGGTGGCCGATGAGCAGCGTGTGCCAGCCCAT
XM_005256149.1      CTCCGCGGGCTCTTCTGCGACGTCGTCCTGGTGGCCGATGAGCAGCGTGTGCCAGCCCAT
                    ************************************************************

HsCD00330102        CGCAACCTGCTGGCCGTGTGCAGCGACTACTTCAACTCCATGTTCACCATCGGCATGCGG
NM_024731.2         CGCAACCTGCTGGCCGTGTGCAGCGACTACTTCAACTCCATGTTCACCATCGGCATGCGG
XM_005256149.1      CGCAACCTGCTGGCCGTGTGCAGCGACTACTTCAACTCCATGTTCACCATCGGCATGCGG
                    ************************************************************

HsCD00330102        GAAGCTTTCCAGAAGGAGGTGGAGCTGATCGGCGCCTCCTACATTGGGCTCAAGGCCGTG
NM_024731.2         GAAGCTTTCCAGAAGGAGGTGGAGCTGATCGGCGCCTCCTACATTGGGCTCAAGGCCGTG
XM_005256149.1      GAAGCTTTCCAGAAGGAGGTGGAGCTGATCGGCGCCTCCTACATTGGGCTCAAGGCCGTG
                    ************************************************************

HsCD00330102        GTGGACTTCCTGTACGGCGGGGAGCTGGTGCTGGATGGCGGCAACATTGACTACGTCCTG
NM_024731.2         GTGGACTTCCTGTACGGCGGGGAGCTGGTGCTGGATGGCGGCAACATTGACTACGTCCTG
XM_005256149.1      GTGGACTTCCTGTACGGCGGGGAGCTGGTGCTGGATGGCGGCAACATTGACTACGTCCTG
                    ************************************************************

HsCD00330102        GAGACGGCTCACCTGCTGCAGATCTGGACGGTGGTAGACTTCTGCTGTGAGTACCTGGAG
NM_024731.2         GAGACGGCTCACCTGCTGCAGATCTGGACGGTGGTAGACTTCTGCTGTGAGTACCTGGAG
XM_005256149.1      GAGACGGCTCACCTGCTGCAGATCTGGACGGTGGTAGACTTCTGCTGTGAGTACCTGGAG
                    ************************************************************

HsCD00330102        CAGGAGGTGAGCGAGGACAACTACCTGTACCTGCAGGAGCTGGCCTCCATCTACAGCCTC
NM_024731.2         CAGGAGGTGAGCGAGGACAACTACCTGTACCTGCAGGAGCTGGCCTCCATCTACAGCCTC
XM_005256149.1      CAGGAGGTGAGCGAGGACAACTACCTGTACCTGCAGGAGCTGGCCTCCATCTACAGCCTC
                    ************************************************************

HsCD00330102        AAGCGGCTTGATGCCTTCATCGATGGCTTCATCCTGAACCACTTCGGCACGCTGTCCTTT
NM_024731.2         AAGCGGCTTGATGCCTTCATCGATGGCTTCATCCTGAACCACTTCGGCACGCTGTCCTTT
XM_005256149.1      AAGCGGCTTGATGCCTTCATCGATGGCTTCATCCTGAACCACTTCGGCACGCTGTCCTTT
                    ************************************************************

HsCD00330102        ACGCCCGACTTCCTGCAGAACGTCTCCATGCAGAAGCTGTGTGTCTACCTGAGCAGCAGC
NM_024731.2         ACGCCCGACTTCCTGCAGAACGTCTCCATGCAGAAGCTGTGTGTCTACCTGAGCAGCAGC
XM_005256149.1      ACGCCCGACTTCCTGCAGAACGTCTCCATGCAGAAGCTGTGTGTCTACCTGAGCAGCAGC
                    ************************************************************

HsCD00330102        GAGGTGCAGCGGGAGTGTGAGCACGACCTCTTGCAGGCCGCCCTGCAGTGGCTGACGCAG
NM_024731.2         GAGGTGCAGCGGGAGTGTGAGCACGACCTCTTGCAGGCCGCCCTGCAGTGGCTGACGCAG
XM_005256149.1      GAGGTGCAGCGGGAGTGTGAGCACGACCTCCTGCAGGCCGCCCTGCAGTGGCTGACGCAG
                    ****************************** *****************************

HsCD00330102        CAGCCCGAGCGCGAGGCCCACGCCCGCCAGGTGCTGGAGAACATCCACTTCCCGCTCATC
NM_024731.2         CAGCCCGAGCGCGAGGCCCACGCCCGCCAGGTGCTGGAGAACATCCACTTCCCGCTCATC
XM_005256149.1      CAGCCCGAGCGCGAGGCCCACGCCCGCCAGGTGCTGGAGAACATCCACTTCCCGCTCATC
                    ************************************************************

HsCD00330102        CCCAAGAACGACCTGCTGCACCGCGTCAAGCCGGCCGTGTGCTCGCTGCTGCCCAAGGAG
NM_024731.2         CCCAAGAACGACCTGCTGCACCGCGTCAAGCCGGCCGTGTGCTCGCTGCTGCCCAAGGAG
XM_005256149.1      CCCAAGAACGACCTGCTGCACCGCGTCAAGCCGGCCGTGTGCTCGCTGCTGCCCAAGGAG
                    ************************************************************

HsCD00330102        GCCAACTGCGAGGGCTTCATCGAGGAGGCCGTGCGCTACCACAACAACCTGGCGGCCCAG
NM_024731.2         GCCAACTGCGAGGGCTTCATCGAGGAGGCCGTGCGCTACCACAACAACCTGGCGGCCCAG
XM_005256149.1      GCCAACTGCGAGGGCTTCATCGAGGAGGCCGTGCGCTACCACAACAACCTGGCGGCCCAG
                    ************************************************************

HsCD00330102        CCCGTCATGCAGACCAAGCGCACGGCGCTGCGCACCAACCAGGAGCGCCTGCTGTTTGTG
NM_024731.2         CCCGTCATGCAGACCAAGCGCACGGCGCTGCGCACCAACCAGGAGCGCCTGCTGTTTGTG
XM_005256149.1      CCCGTCATGCAGACCAAGCGCACGGCGCTGCGCACCAACCAGGAGCGCCTGCTGTTTGTG
                    ************************************************************

HsCD00330102        GGCGGCGAGGTCTCCGAGCGGTGTCTGGAGCTCAGTGACGACACCTGCTACCTGGACGCC
NM_024731.2         GGCGGCGAGGTCTCCGAGCGGTGTCTGGAGCTCAGTGACGACACCTGCTACCTGGACGCC
XM_005256149.1      GGCGGCGAGGTCTCCGAGCGGTGTCTGGAGCTCAGTGACGACACCTGCTACCTGGACGCC
                    ************************************************************

HsCD00330102        AAGAGCGAGCAGTGGGTCAAAGAGACGCCGCTGCCCGCCCGGCGGAGCCACCACTGTGTC
NM_024731.2         AAGAGCGAGCAGTGGGTCAAAGAGACGCCGCTGCCCGCCCGGCGGAGCCACCACTGTGTC
XM_005256149.1      AAGAGCGAGCAGTGGGTCAAAGAGACGCCGCTGCCCGCCCGGCGGAGCCACCACTGTGTC
                    ************************************************************

HsCD00330102        GCGGTGCTGGGGGGCTTCATCTTCATCGCCGGCGGCAGCTTCTCACGGGACAACGGAGGG
NM_024731.2         GCGGTGCTGGGGGGCTTCATCTTCATCGCCGGCGGCAGCTTCTCACGGGACAACGGAGGG
XM_005256149.1      GCGGTGCTGGGGGGCTTCATCTTCATCGCCGGCGGCAGCTTCTCACGGGACAACGGAGGG
                    ************************************************************

HsCD00330102        GATGCGGCCTCCAATCTTCTTTATAGGTATGACCCCCGCTGTAAACAGTGGATCAAGGTG
NM_024731.2         GATGCGGCCTCCAATCTTCTTTATAGGTATGACCCCCGCTGTAAACAGTGGATCAAGGTG
XM_005256149.1      GATGCGGCCTCCAATCTTCTTTATAGGTATGACCCCCGCTGTAAACAGTGGATCAAGGTG
                    ************************************************************

HsCD00330102        GCCTCCATGAACCAGCGCCGTGTGGATTTCTACCTTGCCTCCATCGAAGACATGCTGGTG
NM_024731.2         GCCTCCATGAACCAGCGCCGTGTGGATTTCTACCTTGCCTCCATCGAAGACATGCTGGTG
XM_005256149.1      GCCTCCATGAACCAGCGCCGTGTGGATTTCTACCTTGCCTCCATCGAAGACATGCTGGTG
                    ************************************************************

HsCD00330102        GCCATCGGCGGCCGGAATGAGAACGGAGCGCTCTCTTCAGTAGAGACGTACAGTCCCAAG
NM_024731.2         GCCATCGGCGGCCGGAATGAGAACGGAGCGCTCTCTTCAGTAGAGACGTACAGTCCCAAG
XM_005256149.1      GCCATCGGCGGCCGGAATGAGAACGGAGCGCTCTCTTCAGTAGAGACGTACAGTCCCAAG
                    ************************************************************

HsCD00330102        ACTGACTCCTGGTCCTATGTGGCCGGCTTGCCAAGGTTCACGTACGGCCACGCGGGCACC
NM_024731.2         ACTGACTCCTGGTCCTATGTGGCCGGCTTGCCAAGGTTCACGTACGGCCACGCGGGCACC
XM_005256149.1      ACTGACTCCTGGTCCTATGTGGCCGGCTTGCCAAGGTTCACGTACGGCCACGCGGGCACC
                    ************************************************************

HsCD00330102        ATCTACAAAGACTTCGTGTACATCTCGGGGGGCCACGACTACCAAATTGGCCCCTACCGC
NM_024731.2         ATCTACAAAGACTTCGTGTACATCTCGGGGGGCCACGACTACCAAATTGGCCCCTACCGC
XM_005256149.1      ATCTACAAAGACTTCGTGTACATCTCGGGGGGCCACGACTACCAAATTGGCCCCTACCGC
                    ************************************************************

HsCD00330102        AAGAACCTGCTATGCTACGACCACCGGACAGACGTGTGGGAGGAGCGGCGGCCCATGACC
NM_024731.2         AAGAACCTGCTATGCTACGACCACCGGACAGACGTGTGGGAGGAGCGGCGGCCCATGACC
XM_005256149.1      AAGAACCTGCTATGCTACGACCACCGGACAGACGTGTGGGAGGAGCGGCGGCCCATGACC
                    ************************************************************

HsCD00330102        ACGGCGCGCGGCTGGCACAGCATGTGCAGCCTGGGTGACAGCATCTACTCCATCGGGGGC
NM_024731.2         ACGGCGCGCGGCTGGCACAGCATGTGCAGCCTGGGTGACAGCATCTACTCCATCGGGGGC
XM_005256149.1      ACGGCGCGCGGCTGGCACAGCATGTGCAGCCTGGGTGACAGCATCTACTCCATCGGGGGC
                    ************************************************************

HsCD00330102        AGCGATGACAACATCGAGTCCATGGAGCGCTTCGACGTGCTGGGCGTGGAGGCCTACAGC
NM_024731.2         AGCGATGACAACATCGAGTCCATGGAGCGCTTCGACGTGCTGGGCGTGGAGGCCTACAGC
XM_005256149.1      AGCGATGACAACATCGAGTCCATGGAGCGCTTCGACGTGCTGGGCGTGGAGGCCTACAGC
                    ************************************************************

HsCD00330102        CCGCAGTGCAACCAGTGGACCCGCGTGGCGCCGCTGCTGCACGCCAACAGCGAGTCGGGC
NM_024731.2         CCGCAGTGCAACCAGTGGACCCGCGTGGCGCCGCTGCTGCACGCCAACAGCGAGTCGGGC
XM_005256149.1      CCGCAGTGCAACCAGTGGACCCGCGTGGCGCCGCTGCTGCACGCCAACAGCGAGTCGGGC
                    ************************************************************

HsCD00330102        GTGGCAGTGTGGGAGGGCCGCATCTACATCCTGGGCGGCTACAGCTGGGAGAACACTGCC
NM_024731.2         GTGGCAGTGTGGGAGGGCCGCATCTACATCCTGGGCGGCTACAGCTGGGAGAACACTGCC
XM_005256149.1      GTGGCAGTGTGGGAGGGCCGCATCTACATCCTGGGCGGCTACAGCTGGGAGAACACTGCC
                    ************************************************************

HsCD00330102        TTCTCCAAGACCGTGCAGGTGTACGACCGCGAGGCCGACAAGTGGAGCAGGGGCGTCGAC
NM_024731.2         TTCTCCAAGACCGTGCAGGTGTACGACCGCGAGGCCGACAAGTGGAGCAGGGGCGTCGAC
XM_005256149.1      TTCTCCAAGACCGTGCAGGTGTACGACCGCGAGGCCGACAAGTGGAGCAGGGGCGTCGAC
                    ************************************************************

HsCD00330102        CTGCCCAAGGCCATCGCTGGCGGGTCCGCCTGTGTCTGCGCCCTGGAGCCACGGCCAGAG
NM_024731.2         CTGCCCAAGGCCATCGCTGGCGGGTCCGCCTGTGTCTGCGCCCTGGAGCCACGGCCAGAG
XM_005256149.1      CTGCCCAAGGCCATCGCTGGCGGGTCCGCCTGTGTCTGCGCCCTGGAGCCACGGCCAGAG
                    ************************************************************

HsCD00330102        GACAAGAAGAAGAAAGGCAAAGGCAAGAGGCACCAGGACCGGGGCCAGTTG
NM_024731.2         GACAAGAAGAAGAAAGGCAAAGGCAAGAGGCACCAGGACCGGGGCCAGTGA
XM_005256149.1      GACAAGAAGAAGAAAGGCAAAGGCAAGAGGCACCAGGACCGGGGCCAGTGA
                    ************************************************* .

Protein

SMART domain : BACK : BTB And C-terminal Kelch
SMART domain : Kelch :
SMART domain : BTB : Broad-Complex, Tramtrack and Bric a brac
UniProt : Q8N4N3
HPRD : 12675

Clone

Cloning Information : 268277
HIP Master Clone ID : 195079
Original Clone ID : FLH268277.01L

Organism


TAX_ID : 9606
Species Specific ID: 79786

Genome

Chromosome : 16
Map Location : 16q24.1
Ensembl : ENSG00000135686

Reagents

Labome : KLHL36-antibody