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HTATIP2 (H. sapiens ) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  HTATIP2
Gene Name:  HIV-1 Tat interactive protein 2, 30kDa
Sequence             
Original Clone ID: FLH268327.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00330148

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 729nts         Open reading frame : 1 to 729
Reference Sequence Alignment

ATGGCCGAAACAGAAGCCCTGTCGAAGCTTCGGGAAGACTTCAGGATGCAGAATAAATCC
GTCTTTATTTTGGGCGCCAGCGGAGAAACCGGCAGAGTGCTCTTAAAGGAAATCCTGGAG
CAGGGCCTGTTTTCCAAAGTCACGCTCATTGGCCGGAGGAAGCTCACCTTCGACGAGGAA
GCTTATAAAAATGTGAATCAAGAAGTGGTGGACTTTGAAAAGTTGGATGACTACGCCTCT
GCCTTTCAAGGTCATGATGTTGGATTCTGTTGCCTGGGTACCACCAGAGGGAAAGCTGGG
GCGGAGGGATTTGTTCGTGTTGACCGAGATTATGTGCTGAAGTCTGCAGAGCTGGCAAAA
GCTGGAGGGTGCAAACATTTCAACTTGCTATCCTCTAAAGGAGCTGATAAATCAAGCAAT
TTTTTATATCTACAAGTTAAGGGAGAAGTAGAAGCCAAGGTTGAAGAATTAAAATTTGAT
CGTTACTCTGTATTTAGGCCTGGAGTTCTGTTATGTGATAGGCAAGAATCTCGCCCAGGT
GAATGGCTGGTTAGAAAGTTCTTTGGCTCCTTACCAGACTCTTGGGCCAGGGGGCATTCT
GTGCCTGTGGTGACCGTGGTTAGAGCAATGCTGAACAATGTGGTGAGACCAAGAGACAAG
CAGATGGAACTGCTGGAGAACAAGGCCATCCATGACCTGGGGAAAGCGCATGGCTCTCTC
AAGCCATTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 850
Start on reference sequence 122
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : HTATIP2
Symbol Nomenclature : HTATIP2
SYNONYM : CC3; SDR44U1; TIP30
Designation : 30 kDa HIV-1 TAT-interacting protein|HIV-1 TAT-interactive protein 2|Tat-interacting protein (30kD)|oxidoreductase HTATIP2|short chain dehydrogenase/reductase family 44U, member 1
Full Nomenclature : HIV-1 Tat interactive protein 2, 30kDa
GENEID : 10553
HGNC : 16637
MIM : 605628
Vega : OTTHUMG00000166015
Target GenBank: BC002439

 

Reference Sequence Alignment

  

HsCD00330148        ------------------------------------------------------------
NM_001098522.1      ------------------------------------------------------------
NM_001098521.1      ------------------------------------------------------------
NM_001098520.1      ATGGCCGGGCCTGCGGCGCTGAGCGCGGCGGCGGCGGCTGCTCTGGCGGCCGCCCTGCTC
NM_006410.4         ------------------------------------------------------------
                                                                                

HsCD00330148        ------------------------------------------ATGGCCGAAACAGAAGCC
NM_001098522.1      ------------------------------------------ATGGCCGAAACAGAAGCC
NM_001098521.1      ------------------------------------------ATGGCCGAAACAGAAGCC
NM_001098520.1      CTGCTGCGTCGTGAGGACCCGGGGCCGGGGGCTGGCCCCAGCATGGCCGAAACAGAAGCC
NM_006410.4         ------------------------------------------ATGGCCGAAACAGAAGCC
                                                              ******************

HsCD00330148        CTGTCGAAGCTTCGGGAAGACTTCAGGATGCAGAATAAATCCGTCTTTATTTTGGGCGCC
NM_001098522.1      CTGTCGAAGCTTCGGGAAGACTTCAGGATGCAGAATAAATCCGTCTTTATTTTGGGCGCC
NM_001098521.1      CTGTCGAAGCTTCGGGAAGACTTCAGGATGCAGAATAAATCCGTCTTTATTTTGGGCGCC
NM_001098520.1      CTGTCGAAGCTTCGGGAAGACTTCAGGATGCAGAATAAATCCGTCTTTATTTTGGGCGCC
NM_006410.4         CTGTCGAAGCTTCGGGAAGACTTCAGGATGCAGAATAAATCCGTCTTTATTTTGGGCGCC
                    ************************************************************

HsCD00330148        AGCGGAGAAACCGGCAGAGTGCTCTTAAAGGAAATCCTGGAGCAGGGCCTGTTTTCCAAA
NM_001098522.1      AGCGGAGAAACCGGCAGAGTGCTCTTAAAGGAAATCCTGGAGCAGGGCCTGTTTTCCAAA
NM_001098521.1      AGCGGAGAAACCGGCAGAGTGCTCTTAAAGGAAATCCTGGAGCAGGGCCTGTTTTCCAAA
NM_001098520.1      AGCGGAGAAACCGGCAGAGTGCTCTTAAAGGAAATCCTGGAGCAGGGCCTGTTTTCCAAA
NM_006410.4         AGCGGAGAAACCGGCAGAGTGCTCTTAAAGGAAATCCTGGAGCAGGGCCTGTTTTCCAAA
                    ************************************************************

HsCD00330148        GTCACGCTCATTGGCCGGAGGAAGCTCACCTTCGACGAGGAAGCTTATAAAAATGTGAAT
NM_001098522.1      GTCACGCTCATTGGCCGGAGGAAGCTCACCTTCGACGAGGAAGCTTATAAAAATGTGAAT
NM_001098521.1      GTCACGCTCATTGGCCGGAGGAAGCTCACCTTCGACGAGGAAGCTTATAAAAATGTGAAT
NM_001098520.1      GTCACGCTCATTGGCCGGAGGAAGCTCACCTTCGACGAGGAAGCTTATAAAAATGTGAAT
NM_006410.4         GTCACGCTCATTGGCCGGAGGAAGCTCACCTTCGACGAGGAAGCTTATAAAAATGTGAAT
                    ************************************************************

HsCD00330148        CAAGAAGTGGTGGACTTTGAAAAGTTGGATGACTACGCCTCTGCCTTTCAAGGTCATGAT
NM_001098522.1      CAAGAAGTGGTGGACTTTGAAAAGTTGGATGACTACGCCTCTGCCTTTCAAGGTCATGAT
NM_001098521.1      CAAGAAGTGGTGGACTTTGAAAAGTTGGATGACTACGCCTCTGCCTTTCAAGGTCATGAT
NM_001098520.1      CAAGAAGTGGTGGACTTTGAAAAGTTGGATGACTACGCCTCTGCCTTTCAAGGTCATGAT
NM_006410.4         CAAGAAGTGGTGGACTTTGAAAAGTTGGATGACTACGCCTCTGCCTTTCAAGGTCATGAT
                    ************************************************************

HsCD00330148        GTTGGATTCTGTTGCCTGGGTACCACCAGAGGGAAAGCTGGGGCGGAGGGATTTGTTCGT
NM_001098522.1      GTTGGATTCTGTTGCCTGGGTACCACCAGAGGGAAAGCTGGGGCGGAGGGATTTGTTCGT
NM_001098521.1      GTTGGATTCTGTTGCCTGGGTACCACCAGAGGGAAAGCTGGGGCGGAGGGATTTGTTCGT
NM_001098520.1      GTTGGATTCTGTTGCCTGGGTACCACCAGAGGGAAAGCTGGGGCGGAGGGATTTGTTCGT
NM_006410.4         GTTGGATTCTGTTGCCTGGGTACCACCAGAGGGAAAGCTGGGGCGGAGGGATTTGTTCGT
                    ************************************************************

HsCD00330148        GTTGACCGAGATTATGTGCTGAAGTCTGCAGAGCTGGCAAAAGCTGGAGGGTGCAAACAT
NM_001098522.1      GTTGACCGAGATTATGTGCTGAAGTCTGCAGAGCTGGCAAAAGCTGGAGGGTGCAAACAT
NM_001098521.1      GTTGACCGAGATTATGTGCTGAAGTCTGCAGAGCTGGCAAAAGCTGGAGGGTGCAAACAT
NM_001098520.1      GTTGACCGAGATTATGTGCTGAAGTCTGCAGAGCTGGCAAAAGCTGGAGGGTGCAAACAT
NM_006410.4         GTTGACCGAGATTATGTGCTGAAGTCTGCAGAGCTGGCAAAAGCTGGAGGGTGCAAACAT
                    ************************************************************

HsCD00330148        TTCAACTTGCTATCCTCTAAAGGAGCTGATAAATCAAGCAATTTTTTATATCTACAAGTT
NM_001098522.1      TTCAACTTGCTATCCTCTAAAGGAGCTGATAAATCAAGCAATTTTTTATATCTACAAGTT
NM_001098521.1      TTCAACTTGCTATCCTCTAAAGGAGCTGATAAATCAAGCAATTTTTTATATCTACAAGTT
NM_001098520.1      TTCAACTTGCTATCCTCTAAAGGAGCTGATAAATCAAGCAATTTTTTATATCTACAAGTT
NM_006410.4         TTCAACTTGCTATCCTCTAAAGGAGCTGATAAATCAAGCAATTTTTTATATCTACAAGTT
                    ************************************************************

HsCD00330148        AAGGGAGAAGTAGAAGCCAAGGTTGAAGAATTAAAATTTGATCGTTACTCTGTATTTAGG
NM_001098522.1      AAGGGAGAAGTAGAAGCCAAGGTTGAAGAATTAAAATTTGATCGTTACTCTGTATTTAGG
NM_001098521.1      AAGGGAGAAGTAGAAGCCAAGGTTGAAGAATTAAAATTTGATCGTTACTCTGTATTTAGG
NM_001098520.1      AAGGGAGAAGTAGAAGCCAAGGTTGAAGAATTAAAATTTGATCGTTACTCTGTATTTAGG
NM_006410.4         AAGGGAGAAGTAGAAGCCAAGGTTGAAGAATTAAAATTTGATCGTTACTCTGTATTTAGG
                    ************************************************************

HsCD00330148        CCTGGAGTTCTGTTATGTGATAGGCAAGAATCTCGCCCAGGTGAATGGCTGGTTAGAAAG
NM_001098522.1      CCTGGAGTTCTGTTATGTGATAGGCAAGAATCTCGCCCAGGTGAATGGCTGGTTAGAAAG
NM_001098521.1      CCTGGAGTTCTGTTATGTGATAGGCAAGAATCTCGCCCAGGTGAATGGCTGGTTAGAAAG
NM_001098520.1      CCTGGAGTTCTGTTATGTGATAGGCAAGAATCTCGCCCAGGTGAATGGCTGGTTAGAAAG
NM_006410.4         CCTGGAGTTCTGTTATGTGATAGGCAAGAATCTCGCCCAGGTGAATGGCTGGTTAGAAAG
                    ************************************************************

HsCD00330148        TTCTTTGGCTCCTTACCAGACTCTTGGGCCAGGGGGCATTCTGTGCCTGTGGTGACCGTG
NM_001098522.1      TTCTTTGGCTCCTTACCAGACTCTTGGGCCAGTGGGCATTCTGTGCCTGTGGTGACCGTG
NM_001098521.1      TTCTTTGGCTCCTTACCAGACTCTTGGGCCAGTGGGCATTCTGTGCCTGTGGTGACCGTG
NM_001098520.1      TTCTTTGGCTCCTTACCAGACTCTTGGGCCAGTGGGCATTCTGTGCCTGTGGTGACCGTG
NM_006410.4         TTCTTTGGCTCCTTACCAGACTCTTGGGCCAGTGGGCATTCTGTGCCTGTGGTGACCGTG
                    ******************************** ***************************

HsCD00330148        GTTAGAGCAATGCTGAACAATGTGGTGAGACCAAGAGACAAGCAGATGGAACTGCTGGAG
NM_001098522.1      GTTAGAGCAATGCTGAACAATGTGGTGAGACCAAGAGACAAGCAGATGGAACTGCTGGAG
NM_001098521.1      GTTAGAGCAATGCTGAACAATGTGGTGAGACCAAGAGACAAGCAGATGGAACTGCTGGAG
NM_001098520.1      GTTAGAGCAATGCTGAACAATGTGGTGAGACCAAGAGACAAGCAGATGGAACTGCTGGAG
NM_006410.4         GTTAGAGCAATGCTGAACAATGTGGTGAGACCAAGAGACAAGCAGATGGAACTGCTGGAG
                    ************************************************************

HsCD00330148        AACAAGGCCATCCATGACCTGGGGAAAGCGCATGGCTCTCTCAAGCCATTG
NM_001098522.1      AACAAGGCCATCCATGACCTGGGGAAAGCGCATGGCTCTCTCAAGCCATGA
NM_001098521.1      AACAAGGCCATCCATGACCTGGGGAAAGCGCATGGCTCTCTCAAGCCATGA
NM_001098520.1      AACAAGGCCATCCATGACCTGGGGAAAGCGCATGGCTCTCTCAAGCCATGA
NM_006410.4         AACAAGGCCATCCATGACCTGGGGAAAGCGCATGGCTCTCTCAAGCCATGA
                    ************************************************* .

Clone

Cloning Information : 268327
HIP Master Clone ID : 195134
Original Clone ID : FLH268327.01L

Organism


TAX_ID : 9606
Species Specific ID: 10553

Genome

Chromosome : 11
Map Location : 11p15.1
Ensembl : ENSG00000109854

Reagents

Labome : TIP30-antibody