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HCFC2 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  HCFC2
Gene Name:  host cell factor C2
Sequence             
Original Clone ID: FLH268335.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00330156

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 2379nts         Open reading frame : 1 to 2379
Reference Sequence Alignment

ATGGCGGCTCCCAGCCTCCTCAACTGGAGGCGAGTTTCTTCCTTCACGGGGCCGGTCCCC
CGCGCCCGGCACGGACACCGAGCGGTGGCCATCCGGGAGCTGATGATCATCTTTGGAGGG
GGAAATGAGGGCATCGCGGATGAGCTGCACGTCTACAACACGGCTACGAATCAGTGGTTT
CTGCCAGCTGTTAGAGGAGATATCCCTCCAGGCTGTGCTGCCCATGGATTTGTCTGTGAT
GGTACCAGAATATTAGTATTTGGGGGAATGGTTGAATATGGAAGATACAGCAATGAGTTA
TATGAGTTACAAGCAAGTCGTTGGTTATGGAAAAAAGTGAAACCCCATCCCCCTCCTTCT
GGTTTACCTCCTTGTCCTCGGCTTGGACATAGCTTCTCTTTATATGGTAACAAATGCTAT
TTGTTTGGTGGCCTGGCAAACGAAAGCGAAGATTCAAACAATAATGTTCCCAGATATTTA
AATGATTTTTATGAGTTGGAGCTACAGCATGGCTCTGGTGTTGTGGGTTGGAGCATTCCA
GTGACTAAAGGGGTTGTGCCTTCTCCAAGAGAATCCCACACAGCTGTTATATATTGCAAA
AAAGATTCTGGAAGTCCTAAAATGTATGTTTTTGGTGGAATGTGTGGTGCTCGCCTGGAT
GACCTATGGCAGCTTGACTTAGAAACTATGTCATGGTCAAAACCAGAAACTAAAGGGACA
GTGCCACTTCCACGAAGCCTTCATACAGCCAGTGTTATAGGAAACAAGATGTACATTTTT
GGTGGATGGGTCCCACATAAGGGGGAAAATACTGAGACTTCACCTCATGATTGTGAATGG
AGATGTACCAGTTCATTTTCTTACCTAAATCTGGATACAACAGAGTGGACCACCCTAGTA
TCAGATTCTCAGGAAGATAAAAAAAATTCAAGACCAAGACCAAGAGCTGGCCACTGTGCT
GTTGCAATCGGCACTCGATTGTATTTTTGGAGTGGAAGAGATGGCTACAAAAAAGCACTG
AATAGTCAAGTTTGCTGCAAGGATCTTTGGTATCTTGATACTGAGAAACCACCGGCACCA
TCTCAAGTACAGCTGATCAAAGCCACTACCAACTCCTTTCATGTCAAGTGGGATGAAGTG
TCTACAGTTGAGGGCTATCTTTTGCAGTTGAGTACAGACTTGCCATACCAAGCTGCATCA
TCAGATTCTTCAGCAGCACCAAATATGCAAGGAGTCAGGATGGACCCTCACAGACAAGGC
AGTAATAACATCGTTCCTAACAGTATCAATGATACAATAAACAGCACAAAAACTGAACAG
CCAGCCACAAAAGAAACTTCAATGAAAAACAAACCAGACTTTAAAGCACTGACGGATTCT
AATGCCATTTTATATCCATCTTTGGCATCAAATGCTTCTAATCATAATAGTCATGTGGTG
GATATGCTAAGGAAAAATGAAGGTCCTCACACTTCAGCAAATGTAGGTGTTCTAAGTAGT
TGCCTGGATGTAAGAACAGTAATTCCTGAAACATCTGTATCCAGTACTGTTTCCAGCACA
CAAACTATGGTAACCCAGCAGACCATTAAAACTGAATCATCCAGTACAAATGGGGCAGTT
GTTAAAGATGAAACTTCACTAACAACATTCAGTACCAAATCTGAAGTTGATGAAACATAT
GCACTGCCTGCAACGAAGATCAGCCGTGTAGAGACACATGCTACAGCAACGCCGTTTTCT
AAAGAGACTCCTTCAAATCCAGTGGCCACAGTGAAAGCGGGAGAACGACAATGGTGTGAT
GTGGGAATTTTTAAAAATAATACAGCTTTGGTGAGCCAGTTTTATTTGCTGCCAAAAGGG
AAGCAAAGCATCTCAAAGGTAGGAAATGCAGATGTACCTGACTACAGCTTGCTTAAGAAA
CAAGATCTTGTTCCAGGCACAGGATACAGATTCAGGGTTGCTGCAATCAATGGTTGTGGG
ATAGGTCCTTTCAGCAAAATCAGTGAATTTAAAACTTGTATTCCTGGTTTTCCTGGAGCT
CCTTCTGCAGTCAGAATTTCAAAGAATGTTGAAGGTATCCACCTTTCCTGGGAACCTCCA
ACCTCACCTTCTGGAAATATTTTGGAATATTCAGCCTACTTGGCTATCCGCACAGCACAG
ATACAAGATAATCCAAGTCAACTTGTGTTCATGAGGATTTATTGTGGTCTTAAGACATCA
TGTATAGTAACTGCTGGGCAACTTGCAAATGCACATATTGATTATACATCCAGGCCTGCC
ATTGTGTTCAGGATATCAGCAAAGAATGAAAAGGGATATGGACCAGCTACACAAGTTCGG
TGGCTTCAAGGTAACAATAAGAAAGCACCTTTAAATTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 2384
Start on reference sequence 6
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: with tag/fusion/marker assay, Gateway®, acceptor (destination), recombinational cloning clone, lentiviral infection, mammalian expression expression with CMV and PGK and BlastR and AmpR and RSV and 5' splice donor and HIV-1 psi and HIV-1 RRE and 3' splice acceptor and V5 and SV40 and pUC ori and attR1 and attR2 and ccdB and CmR; blasticidin resistance in mammalian, ampicillin resistance in bacterial;
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR1 attR1 recombinational cloning site 2440 2456
recombination site attR2 attR2 recombinational cloning site 4127 4142
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : HCFC2
Symbol Nomenclature : HCFC2
SYNONYM : HCF-2; HCF2
Designation : C2 factor|host cell factor 2
Full Nomenclature : host cell factor C2
GENEID : 29915
HGNC : 24972
MIM : 607926
Target GenBank: BC033799

 

Reference Sequence Alignment

  

HsCD00330156      ATGGCGGCTCCCAGCCTCCTCAACTGGAGGCGAGTTTCTTCCTTCACGGGGCCGGTCCCC
NM_013320.2       ATGGCGGCTCCCAGCCTCCTCAACTGGAGGCGAGTTTCTTCCTTCACGGGGCCGGTCCCC
                  ************************************************************

HsCD00330156      CGCGCCCGGCACGGACACCGAGCGGTGGCCATCCGGGAGCTGATGATCATCTTTGGAGGG
NM_013320.2       CGCGCCCGGCACGGACACCGAGCGGTGGCCATCCGGGAGCTGATGATCATCTTTGGAGGG
                  ************************************************************

HsCD00330156      GGAAATGAGGGCATCGCGGATGAGCTGCACGTCTACAACACGGCTACGAATCAGTGGTTT
NM_013320.2       GGAAATGAGGGCATCGCGGATGAGCTGCACGTCTACAACACGGCTACGAATCAGTGGTTT
                  ************************************************************

HsCD00330156      CTGCCAGCTGTTAGAGGAGATATCCCTCCAGGCTGTGCTGCCCATGGATTTGTCTGTGAT
NM_013320.2       CTGCCAGCTGTTAGAGGAGATATCCCTCCAGGCTGTGCTGCCCATGGATTTGTCTGTGAT
                  ************************************************************

HsCD00330156      GGTACCAGAATATTAGTATTTGGGGGAATGGTTGAATATGGAAGATACAGCAATGAGTTA
NM_013320.2       GGTACCAGAATATTAGTATTTGGGGGAATGGTTGAATATGGAAGATACAGCAATGAGTTA
                  ************************************************************

HsCD00330156      TATGAGTTACAAGCAAGTCGTTGGTTATGGAAAAAAGTGAAACCCCATCCCCCTCCTTCT
NM_013320.2       TATGAGTTACAAGCAAGTCGTTGGTTATGGAAAAAAGTGAAACCCCATCCCCCTCCTTCT
                  ************************************************************

HsCD00330156      GGTTTACCTCCTTGTCCTCGGCTTGGACATAGCTTCTCTTTATATGGTAACAAATGCTAT
NM_013320.2       GGTTTACCTCCTTGTCCTCGGCTTGGACATAGCTTCTCTTTATATGGTAACAAATGCTAT
                  ************************************************************

HsCD00330156      TTGTTTGGTGGCCTGGCAAACGAAAGCGAAGATTCAAACAATAATGTTCCCAGATATTTA
NM_013320.2       TTGTTTGGTGGCCTGGCAAACGAAAGCGAAGATTCAAACAATAATGTTCCCAGATATTTA
                  ************************************************************

HsCD00330156      AATGATTTTTATGAGTTGGAGCTACAGCATGGCTCTGGTGTTGTGGGTTGGAGCATTCCA
NM_013320.2       AATGATTTTTATGAGTTGGAGCTACAGCATGGCTCTGGTGTTGTGGGTTGGAGCATTCCA
                  ************************************************************

HsCD00330156      GTGACTAAAGGGGTTGTGCCTTCTCCAAGAGAATCCCACACAGCTGTTATATATTGCAAA
NM_013320.2       GTGACTAAAGGGGTTGTGCCTTCTCCAAGAGAATCCCACACAGCTGTTATATATTGCAAA
                  ************************************************************

HsCD00330156      AAAGATTCTGGAAGTCCTAAAATGTATGTTTTTGGTGGAATGTGTGGTGCTCGCCTGGAT
NM_013320.2       AAAGATTCTGGAAGTCCTAAAATGTATGTTTTTGGTGGAATGTGTGGTGCTCGCCTGGAT
                  ************************************************************

HsCD00330156      GACCTATGGCAGCTTGACTTAGAAACTATGTCATGGTCAAAACCAGAAACTAAAGGGACA
NM_013320.2       GACCTATGGCAGCTTGACTTAGAAACTATGTCATGGTCAAAACCAGAAACTAAAGGGACA
                  ************************************************************

HsCD00330156      GTGCCACTTCCACGAAGCCTTCATACAGCCAGTGTTATAGGAAACAAGATGTACATTTTT
NM_013320.2       GTGCCACTTCCACGAAGCCTTCATACAGCCAGTGTTATAGGAAACAAGATGTACATTTTT
                  ************************************************************

HsCD00330156      GGTGGATGGGTCCCACATAAGGGGGAAAATACTGAGACTTCACCTCATGATTGTGAATGG
NM_013320.2       GGTGGATGGGTCCCACATAAGGGGGAAAATACTGAGACTTCACCTCATGATTGTGAATGG
                  ************************************************************

HsCD00330156      AGATGTACCAGTTCATTTTCTTACCTAAATCTGGATACAACAGAGTGGACCACCCTAGTA
NM_013320.2       AGATGTACCAGTTCATTTTCTTACCTAAATCTGGATACAACAGAGTGGACCACCCTAGTA
                  ************************************************************

HsCD00330156      TCAGATTCTCAGGAAGATAAAAAAAATTCAAGACCAAGACCAAGAGCTGGCCACTGTGCT
NM_013320.2       TCAGATTCTCAGGAAGATAAAAAAAATTCAAGACCAAGACCAAGAGCTGGCCACTGTGCT
                  ************************************************************

HsCD00330156      GTTGCAATCGGCACTCGATTGTATTTTTGGAGTGGAAGAGATGGCTACAAAAAAGCACTG
NM_013320.2       GTTGCAATCGGCACTCGATTGTATTTTTGGAGTGGAAGAGATGGCTACAAAAAAGCACTG
                  ************************************************************

HsCD00330156      AATAGTCAAGTTTGCTGCAAGGATCTTTGGTATCTTGATACTGAGAAACCACCGGCACCA
NM_013320.2       AATAGTCAAGTTTGCTGCAAGGATCTTTGGTATCTTGATACTGAGAAACCACCGGCACCA
                  ************************************************************

HsCD00330156      TCTCAAGTACAGCTGATCAAAGCCACTACCAACTCCTTTCATGTCAAGTGGGATGAAGTG
NM_013320.2       TCTCAAGTACAGCTGATCAAAGCCACTACCAACTCCTTTCATGTCAAGTGGGATGAAGTG
                  ************************************************************

HsCD00330156      TCTACAGTTGAGGGCTATCTTTTGCAGTTGAGTACAGACTTGCCATACCAAGCTGCATCA
NM_013320.2       TCTACAGTTGAGGGCTATCTTTTGCAGTTGAGTACAGACTTGCCATACCAAGCTGCATCA
                  ************************************************************

HsCD00330156      TCAGATTCTTCAGCAGCACCAAATATGCAAGGAGTCAGGATGGACCCTCACAGACAAGGC
NM_013320.2       TCAGATTCTTCAGCAGCACCAAATATGCAAGGAGTCAGGATGGACCCTCACAGACAAGGC
                  ************************************************************

HsCD00330156      AGTAATAACATCGTTCCTAACAGTATCAATGATACAATAAACAGCACAAAAACTGAACAG
NM_013320.2       AGTAATAACATCGTTCCTAACAGTATCAATGATACAATAAACAGCACAAAAACTGAACAG
                  ************************************************************

HsCD00330156      CCAGCCACAAAAGAAACTTCAATGAAAAACAAACCAGACTTTAAAGCACTGACGGATTCT
NM_013320.2       CCAGCCACAAAAGAAACTTCAATGAAAAACAAACCAGACTTTAAAGCACTGACGGATTCT
                  ************************************************************

HsCD00330156      AATGCCATTTTATATCCATCTTTGGCATCAAATGCTTCTAATCATAATAGTCATGTGGTG
NM_013320.2       AATGCCATTTTATATCCATCTTTGGCATCAAATGCTTCTAATCATAATAGTCATGTGGTG
                  ************************************************************

HsCD00330156      GATATGCTAAGGAAAAATGAAGGTCCTCACACTTCAGCAAATGTAGGTGTTCTAAGTAGT
NM_013320.2       GATATGCTAAGGAAAAATGAAGGTCCTCACACTTCAGCAAATGTAGGTGTTCTAAGTAGT
                  ************************************************************

HsCD00330156      TGCCTGGATGTAAGAACAGTAATTCCTGAAACATCTGTATCCAGTACTGTTTCCAGCACA
NM_013320.2       TGCCTGGATGTAAGAACAGTAATTCCTGAAACATCTGTATCCAGTACTGTTTCCAGCACA
                  ************************************************************

HsCD00330156      CAAACTATGGTAACCCAGCAGACCATTAAAACTGAATCATCCAGTACAAATGGGGCAGTT
NM_013320.2       CAAACTATGGTAACCCAGCAGACCATTAAAACTGAATCATCCAGTACAAATGGGGCAGTT
                  ************************************************************

HsCD00330156      GTTAAAGATGAAACTTCACTAACAACATTCAGTACCAAATCTGAAGTTGATGAAACATAT
NM_013320.2       GTTAAAGATGAAACTTCACTAACAACATTCAGTACCAAATCTGAAGTTGATGAAACATAT
                  ************************************************************

HsCD00330156      GCACTGCCTGCAACGAAGATCAGCCGTGTAGAGACACATGCTACAGCAACGCCGTTTTCT
NM_013320.2       GCACTGCCTGCAACGAAGATCAGCCGTGTAGAGACACATGCTACAGCAACGCCGTTTTCT
                  ************************************************************

HsCD00330156      AAAGAGACTCCTTCAAATCCAGTGGCCACAGTGAAAGCGGGAGAACGACAATGGTGTGAT
NM_013320.2       AAAGAGACTCCTTCAAATCCAGTGGCCACAGTGAAAGCGGGAGAACGACAATGGTGTGAT
                  ************************************************************

HsCD00330156      GTGGGAATTTTTAAAAATAATACAGCTTTGGTGAGCCAGTTTTATTTGCTGCCAAAAGGG
NM_013320.2       GTGGGAATTTTTAAAAATAATACAGCTTTGGTGAGCCAGTTTTATTTGCTGCCAAAAGGG
                  ************************************************************

HsCD00330156      AAGCAAAGCATCTCAAAGGTAGGAAATGCAGATGTACCTGACTACAGCTTGCTTAAGAAA
NM_013320.2       AAGCAAAGCATCTCAAAGGTAGGAAATGCAGATGTACCTGACTACAGCTTGCTTAAGAAA
                  ************************************************************

HsCD00330156      CAAGATCTTGTTCCAGGCACAGGATACAGATTCAGGGTTGCTGCAATCAATGGTTGTGGG
NM_013320.2       CAAGATCTTGTTCCAGGCACAGGATACAGATTCAGGGTTGCTGCAATCAATGGTTGTGGG
                  ************************************************************

HsCD00330156      ATAGGTCCTTTCAGCAAAATCAGTGAATTTAAAACTTGTATTCCTGGTTTTCCTGGAGCT
NM_013320.2       ATAGGTCCTTTCAGCAAAATCAGTGAATTTAAAACTTGTATTCCTGGTTTTCCTGGAGCT
                  ************************************************************

HsCD00330156      CCTTCTGCAGTCAGAATTTCAAAGAATGTTGAAGGTATCCACCTTTCCTGGGAACCTCCA
NM_013320.2       CCTTCTGCAGTCAGAATTTCAAAGAATGTTGAAGGTATCCACCTTTCCTGGGAACCTCCA
                  ************************************************************

HsCD00330156      ACCTCACCTTCTGGAAATATTTTGGAATATTCAGCCTACTTGGCTATCCGCACAGCACAG
NM_013320.2       ACCTCACCTTCTGGAAATATTTTGGAATATTCAGCCTACTTGGCTATCCGCACAGCACAG
                  ************************************************************

HsCD00330156      ATACAAGATAATCCAAGTCAACTTGTGTTCATGAGGATTTATTGTGGTCTTAAGACATCA
NM_013320.2       ATACAAGATAATCCAAGTCAACTTGTGTTCATGAGGATTTATTGTGGTCTTAAGACATCA
                  ************************************************************

HsCD00330156      TGTATAGTAACTGCTGGGCAACTTGCAAATGCACATATTGATTATACATCCAGGCCTGCC
NM_013320.2       TGTATAGTAACTGCTGGGCAACTTGCAAATGCACATATTGATTATACATCCAGGCCTGCC
                  ************************************************************

HsCD00330156      ATTGTGTTCAGGATATCAGCAAAGAATGAAAAGGGATATGGACCAGCTACACAAGTTCGG
NM_013320.2       ATTGTGTTCAGGATATCAGCAAAGAATGAAAAGGGATATGGACCAGCTACACAAGTTCGG
                  ************************************************************

HsCD00330156      TGGCTTCAAGGTAACAATAAGAAAGCACCTTTAAATTTG
NM_013320.2       TGGCTTCAAGGTAACAATAAGAAAGCACCTTTAAATTGA
                  ************************************* .

Protein

SMART domain : FN3 : Fibronectin type 3 domain
UniProt : Q9Y5Z7
HPRD : 06393

Clone

Cloning Information : 268335
HIP Master Clone ID : 195142
Original Clone ID : FLH268335.01L

Organism


TAX_ID : 9606
Species Specific ID: 29915

Genome

Chromosome : 12
Map Location : 12q23.3

Reagents

Labome : HCF-2-antibody