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POLB (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  POLB
Gene Name:  polymerase (DNA directed), beta
Sequence             
Original Clone ID: FLH269516.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00330193

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1008nts        
Open reading frame : 1 to 1008
Reference Sequence Alignment

ATGAGCAAACGGAAGGCGCCGCAGGAGACTCTCAACGGGGGAATCACCGACATGCTCACA
GAACTCGCAAACTTTGAGAAGAACGTGAGCCAAGCTATCCACAAGTACAATGCTTACAGA
AAAGCAGCATCTGTTATAGCAAAATACCCACACAAAATAAAGAGTGGAGCTGAAGCTAAG
AAATTGCCTGGAGTAGGAACAAAAATTGCTGAAAAGATTGATGAGTTTTTAGCAACTGGA
AAATTACGTAAACTGGAAAAGATTCGGCAGGATGATACGAGTTCATCCATCAATTTCCTG
ACTCGAGTTAGTGGCATTGGTCCATCTGCTGCAAGGAAGTTTGTAGATGAAGGAATTAAA
ACACTAGAAGATCTCAGAAAAAATGAAGATAAATTGAACCATCATCAGCGAATTGGGCTG
AAATATTTTGGGGACTTTGAAAAAAGAATTCCTCGTGAAGAGATGTTACAAATGCAAGAT
ATTGTACTAAATGAAGTTAAAAAAGTGGATTCTGAATACATTGCTACAGTCTGTGGCAGT
TTCAGAAGAGGTGCAGAGTCCAGTGGTGACATGGATGTTCTCCTGACCCATCCCAGCTTC
ACTTCAGAATCAACCAAACAGCCAAAACTGTTACATCAGGTTGTGGAGCAGTTACAAAAG
GTTCATTTTATCACAGATACCCTGTCAAAGGGTGAGACAAAGTTCATGGGTGTTTGCCAG
CTTCCCAGTAAAAATGATGAAAAAGAATATCCACACAGAAGAATTGATATCAGGTTGATA
CCCAAAGATCAGTATTACTGTGGTGTTCTCTATTTCACTGGGAGTGATATTTTCAATAAG
AATATGAGGGCTCATGCCCTAGAAAAGGGTTTCACAATCAATGAGTACACCATCCGTCCC
TTGGGAGTCACTGGAGTTGCAGGAGAACCCCTGCCAGTGGATAGTGAAAAAGACATCTTT
GATTACATCCAGTGGAAATACCGGGAACCCAAGGACCGGAGCGAAGAC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1121
Start on reference sequence 114
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: with tag/fusion/marker assay, Gateway®, acceptor (destination), recombinational cloning clone, lentiviral infection, mammalian expression expression with CMV and PGK and BlastR and AmpR and RSV and 5' splice donor and HIV-1 psi and HIV-1 RRE and 3' splice acceptor and V5 and SV40 and pUC ori and attR1 and attR2 and ccdB and CmR; blasticidin resistance in mammalian, ampicillin resistance in bacterial;
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
promoter RSV RSV/5' LTR hybrid promoter 1 410
recombination site attR1 attR1 recombinational cloning site 2440 2456
recombination site attR2 attR2 recombinational cloning site 4127 4142
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : POLB
Symbol Nomenclature : POLB
Designation : DNA pol beta|DNA polymerase beta|DNA polymerase beta subunit|mutant DNA polymerase beta
Full Nomenclature : polymerase (DNA directed), beta
GENEID : 5423
HGNC : 9174
MIM : 174760
Vega : OTTHUMG00000164093
Target GenBank: NM_002690

 

Reference Sequence Alignment

  

HsCD00330193        ATGAGCAAACGGAAGGCGCCGCAGGAGACTCTCAACGGGGGAATCACCGACATGCTCACA
XM_005273537.1      ATGAGCAAACGGAAGGCGCCGCAGGAGACTCTCAACGGGGGAATCACCGACATGCTCACA
XM_005273535.1      ATGAGCAAACGGAAGGCGCCGCAGGAGACTCTCAACGGGGGAATCACCGACATGCTCACA
NM_002690.2         ATGAGCAAACGGAAGGCGCCGCAGGAGACTCTCAACGGGGGAATCACCGACATGCTCACA
                    ************************************************************

HsCD00330193        GAACTCGCAAACTTTGAGAAGAACGTGAGCCAAGCTATCCACAAGTACAATGCTTACAGA
XM_005273537.1      GAACTCGCAAACTTTGAGAAGAACGTGAGCCAAGCTATCCACAAGTACAATGCTTACAGA
XM_005273535.1      GAACTCGCAAACTTTGAGAAGAACGTGAGCCAAGCTATCCACAAGTACAATGCTTACAGA
NM_002690.2         GAACTCGCAAACTTTGAGAAGAACGTGAGCCAAGCTATCCACAAGTACAATGCTTACAGA
                    ************************************************************

HsCD00330193        AAAGCAGCATCTGTTATAGCAAAATACCCACACAAAATAAAGAGTGGAGCTGAAGCTAAG
XM_005273537.1      AAAGCAGCATCTGTTATAGCAAAATACCCACACAAAATAAAGAGTGGAGCTGAAGCTAAG
XM_005273535.1      AAAGCAGCATCTGTTATAGCAAAATACCCACACAAAATAAAGAGTGGAGCTGAAGCTAAG
NM_002690.2         AAAGCAGCATCTGTTATAGCAAAATACCCACACAAAATAAAGAGTGGAGCTGAAGCTAAG
                    ************************************************************

HsCD00330193        AAATTGCCTGGAGTAGGAACAAAAATTGCTGAAAAGATTGATGAGTTTTTAGCAACTGGA
XM_005273537.1      AAATTGCCTGGAGTAGGAACAAAAATTGCTGAAAAGATTGATGAGTTTTTAGCAACTGGA
XM_005273535.1      AAATTGCCTGGAGTAGGAACAAAAATTGCTGAAAAGATTGATGAGTTTTTAGCAACTGGA
NM_002690.2         AAATTGCCTGGAGTAGGAACAAAAATTGCTGAAAAGATTGATGAGTTTTTAGCAACTGGA
                    ************************************************************

HsCD00330193        AAATTACGTAAACTGGAAAAGATTCGGCAGGATGATACGAGTTCATCCATCAATTTCCTG
XM_005273537.1      AAATTACGTAAACTGGAAAAGATTCGGCAGGATGATACGAGTTCATCCATCAATTTCCTG
XM_005273535.1      AAATTACGTAAACTGGAAAAGATTCGGCAGGATGATACGAGTTCATCCATCAATTTCCTG
NM_002690.2         AAATTACGTAAACTGGAAAAGATTCGGCAGGATGATACGAGTTCATCCATCAATTTCCTG
                    ************************************************************

HsCD00330193        ACTCGAGTTAGTGGCATTGGTCCATCTGCTGCAAGGAAGTTTGTAGATGAAGGAATTAAA
XM_005273537.1      ACTCGAGTTAGTGGCATTGGTCCATCTGCTGCAAGGAAGTTTGTAGATGAAGGAATTAAA
XM_005273535.1      ACTCGAGTTAGTGGCATTGGTCCATCTGCTGCAAGGAAGTTTGTAGATGAAGGAATTAAA
NM_002690.2         ACTCGAGTTAGTGGCATTGGTCCATCTGCTGCAAGGAAGTTTGTAGATGAAGGAATTAAA
                    ************************************************************

HsCD00330193        ACACTAGAAG--------------------------------------------------
XM_005273537.1      ACACTAG---AAG-----------------------------------------------
XM_005273535.1      ACACTAG---AAGGCTCACAGCTGGATTCATGCCCAGTAAAGGGACACCTGAATGGAACT
NM_002690.2         ACACTAG---AAG-----------------------------------------------
                    *******                                                     

HsCD00330193        ----------------------------------------------------------AT
XM_005273537.1      ----------------------------------------------------------AT
XM_005273535.1      GAGTCACTTTTAGACTTAATATGGGATGTTATGACAATTCTTAAGTTAAAAAATGCAGAT
NM_002690.2         ----------------------------------------------------------AT
                                                                              **

HsCD00330193        CTCAGAAAAAATGAAGATAAATTGAACCATCATCAGCGAATTGGGCTGAAATATTTTGGG
XM_005273537.1      CTCAGAAAAAATGAAGATAAATTGAACCATCATCAGCGAATTGGGCTGAAATATTTTGGG
XM_005273535.1      CTCAGAAAAAATGAAGATAAATTGAACCATCATCAGCGAATTGGGCTGAAATATTTTGGG
NM_002690.2         CTCAGAAAAAATGAAGATAAATTGAACCATCATCAGCGAATTGGGCTGAAATATTTTGGG
                    ************************************************************

HsCD00330193        GACTTTGAAAAAAGAATTCCTCGTGAAGAGATGTTACAAATGCAAGATATTGTACTAAAT
XM_005273537.1      GACTTTGAAAAAAGAATTCCTCGTGAAGAGATGTTACAAATGCAAGATATTGTACTAAAT
XM_005273535.1      GACTTTGAAAAAAGAATTCCTCGTGAAGAGATGTTACAAATGCAAGATATTGTACTAAAT
NM_002690.2         GACTTTGAAAAAAGAATTCCTCGTGAAGAGATGTTACAAATGCAAGATATTGTACTAAAT
                    ************************************************************

HsCD00330193        GAAGTTAAAAAAGTGGATTCTGAATACATTGCTACAGTCTGTGGCAGTTTCAGAAGAGGT
XM_005273537.1      GAAGTTAAAAAAGTGGATTCTGAATACATTGCTACAGTCTGTGGCAGTTTCAGAAGAGGT
XM_005273535.1      GAAGTTAAAAAAGTGGATTCTGAATACATTGCTACAGTCTGTGGCAGTTTCAGAAGAGGT
NM_002690.2         GAAGTTAAAAAAGTGGATTCTGAATACATTGCTACAGTCTGTGGCAGTTTCAGAAGAGGT
                    ************************************************************

HsCD00330193        GCAGAGTCCAGTGGTGACATGGATGTTCTCCTGACCCATCCCAGCTTCACTTCAGAATCA
XM_005273537.1      GCAGAGTCCAGTGGTGACATGGATGTTCTCCTGACCCATCCCAGCTTCACTTCAGAATCA
XM_005273535.1      GCAGAGTCCAGTGGTGACATGGATGTTCTCCTGACCCATCCCAGCTTCACTTCAGAATCA
NM_002690.2         GCAGAGTCCAGTGGTGACATGGATGTTCTCCTGACCCATCCCAGCTTCACTTCAGAATCA
                    ************************************************************

HsCD00330193        ACCAAACAGCCAAAACTGTTACATCAGGTTGTGGAGCAGTTACAAAAGGTTCATTTTATC
XM_005273537.1      ACCAAACAG---------------------------------------------------
XM_005273535.1      ACCAAACAGCCAAAACTGTTACATCAGGTTGTGGAGCAGTTACAAAAGGTTCATTTTATC
NM_002690.2         ACCAAACAGCCAAAACTGTTACATCAGGTTGTGGAGCAGTTACAAAAGGTTCATTTTATC
                    *********                                                   

HsCD00330193        ACAGATACCCTGTCAAAGGGTGAGACAAAGTTCATGGGTGTTTGCCAGCTTCCCAGTAAA
XM_005273537.1      ------------------------------------GGTGTTTGCCAGCTTCCCAGTAAA
XM_005273535.1      ACAGATACCCTGTCAAAGGGTGAGACAAAGTTCATGGGTGTTTGCCAGCTTCCCAGTAAA
NM_002690.2         ACAGATACCCTGTCAAAGGGTGAGACAAAGTTCATGGGTGTTTGCCAGCTTCCCAGTAAA
                                                        ************************

HsCD00330193        AATGATGAAAAAGAATATCCACACAGAAGAATTGATATCAGGTTGATACCCAAAGATCAG
XM_005273537.1      AATGATGAAAAAGAATATCCACACAGAAGAATTGATATCAGGTTGATACCCAAAGATCAG
XM_005273535.1      AATGATGAAAAAGAATATCCACACAGAAGAATTGATATCAGGTTGATACCCAAAGATCAG
NM_002690.2         AATGATGAAAAAGAATATCCACACAGAAGAATTGATATCAGGTTGATACCCAAAGATCAG
                    ************************************************************

HsCD00330193        TATTACTGTGGTGTTCTCTATTTCACTGGGAGTGATATTTTCAATAAGAATATGAGGGCT
XM_005273537.1      TATTACTGTGGTGTTCTCTATTTCACTGGGAGTGATATTTTCAATAAGAATATGAGGGCT
XM_005273535.1      TATTACTGTGGTGTTCTCTATTTCACTGGGAGTGATATTTTCAATAAGAATATGAGGGCT
NM_002690.2         TATTACTGTGGTGTTCTCTATTTCACTGGGAGTGATATTTTCAATAAGAATATGAGGGCT
                    ************************************************************

HsCD00330193        CATGCCCTAGAAAAGGGTTTCACAATCAATGAGTACACCATCCGTCCCTTGGGAGTCACT
XM_005273537.1      CATGCCCTAGAAAAGGGTTTCACAATCAATGAGTACACCATCCGTCCCTTGGGAGTCACT
XM_005273535.1      CATGCCCTAGAAAAGGGTTTCACAATCAATGAGTACACCATCCGTCCCTTGGGAGTCACT
NM_002690.2         CATGCCCTAGAAAAGGGTTTCACAATCAATGAGTACACCATCCGTCCCTTGGGAGTCACT
                    ************************************************************

HsCD00330193        GGAGTTGCAGGAGAACCCCTGCCAGTGGATAGTGAAAAAGACATCTTTGATTACATCCAG
XM_005273537.1      GGAGTTGCAGGAGAACCCCTGCCAGTGGATAGTGAAAAAGACATCTTTGATTACATCCAG
XM_005273535.1      GGAGTTGCAGGAGAACCCCTGCCAGTGGATAGTGAAAAAGACATCTTTGATTACATCCAG
NM_002690.2         GGAGTTGCAGGAGAACCCCTGCCAGTGGATAGTGAAAAAGACATCTTTGATTACATCCAG
                    ************************************************************

HsCD00330193        TGGAAATACCGGGAACCCAAGGACCGGAGCGAAGAC
XM_005273537.1      TGGAAATACCGGGAACCCAAGGACCGGAGCGAATGA
XM_005273535.1      TGGAAATACCGGGAACCCAAGGACCGGAGCGAATGA
NM_002690.2         TGGAAATACCGGGAACCCAAGGACCGGAGCGAATGA
                    ********************************* ..

Pathways

Reactome : Base Excision Repair
Reactome : Base-free sugar-phosphate removal via the single-nucleotide replacement pathway
Reactome : DNA Repair
Reactome : Removal of DNA patch containing abasic residue
Reactome : Resolution of Abasic Sites (AP sites)
Reactome : Resolution of AP sites via the multiple-nucleotide patch replacement pathway
Reactome : Resolution of AP sites via the single-nucleotide replacement pathway
WikiPathway : Nucleotide Metabolism

Protein

SMART domain : POLXc : DNA polymerase X family
UniProt : P06746
HPRD : 07517

Clone

Cloning Information : 269516
HIP Master Clone ID : 107118
Original Clone ID : FLH269516.01L

Organism


TAX_ID : 9606
Species Specific ID: 5423

Genome

Chromosome : 8
Map Location : 8p11.2
Ensembl : ENSG00000070501