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Sden_0707 (Shewanella denitrificans OS217) in pSpeedET (bacterial expression vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  Sden_0707
Gene Name:  None
Sequence             
Original Clone ID: 49083
Keyword: SP or TM removed
Species: Shewanella denitrificans OS217
Type: cDNA
Vector Name: pSpeedET               Format:  CLOSED
Source: Joint Center for Structural Genomics
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: PSI
Joint Center for Structural Genomics
SdCD00333148

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AVAILABLE
No restriction
Special MTA: PSI

 

Insert sequence: 849nts         Open reading frame : 1 to 849

GTCGAATACCCGTTACCAGCCAAGAACAGTAACTTGGTAGGTGAGAACCAATATTATGTG
GTCCCCGAAGGCAAGTTGACCTTGGAAGCCATTGCCACTAAATTTCAGCTGGGATTAACT
AACCTGCTGGAAGCTAATCCAGGTGTTGACCCTTTCTTGCCAAAACCTGGAAGTACCTTA
GTGATCCCGCACCAGTTGATTTTACCTAACGCCCCCCGTGAAGGCATAGTGATCAATGTG
GCTGAAATGCGCCTCTACTATTACCCTAAGGGTAAAAATATCGTTGAAGTTTTGCCTATC
GGAATAGGACAAATTGGTAAAGACACCCCTGAAAAATGGGTGACTACAGTGCAGCGTAAG
CGTGCTAACCCAACCTGGACACCGACACCGCGCATTCGTAAAGAGTACCTTGCAAAAGGT
GAGGTGCTACCTGCAGTTTGGCCAGCAGGTCCTGACAACCCAATGGGACTGTTTGCACTT
TACATAGGTAATTTGTATGCCATCCACGGTACCAATGCCACCTTTGGTATTGGTCTGCGC
ATCAGCCAAGGCTGTATTCGTTTACGTGATGCCGACATCGAGCACTTATTTAATAGCGTA
CCTGAAGGCACACGCGTGGAGTTCACTAACCAGCCTATCAAGGCCAGTGAAGAGCCTGAT
GGTAATCGTTACTTAGAAGTGCATCAGCCACTGTCACGGACTATGGCTGAATATGAGTCA
GCTGAACCTGTGGGACTTAGCATGCATAGGGATATAGCAAAGTTTGTTGCGCATGCGGAC
ACAGATTCTTTTGTGTTAAAACGCTTGCTCGAACAACGTAACGGCATCCCTACTCGCATC
AATCAATAA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in 396440
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pSpeedET

pSpeedET in advanced viewed

Synonyms: pSpeed-ET
Sequencing Primer: Forward:  pBAD forward
Reverse:  alt_bad_rev
Description: Bacterial expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHHENLYFQG tag; kanamycin resistance; PIPE cloning.
Comments: If this vector contains an insert, the ccdB death cassette will not be present.
Size (bp): 5464
Parent Vector: None
Empty Vector: EvNO00285791
Properties: PIPE cloning, bacterial expression, in vitro transcription, inducible expression, with tag/fusion/marker
Author Name: Joint Center for Structural Genomics
Publications: PMID: 18004753
Title: Combining the polymerase incomplete primer extension method for cloning and mutagenesis with microscreening to accelerate structural genomics efforts.

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori ColE1-type origin of replication 3306 3988
inducible trxn activator protein gene araC araC coding sequence 4563 5438
negative selection marker ccdB ccdB death cassette gene (lost in with-insert form) 1596 1805
promoter T7 T7 promoter 290 310
promoter araC araC promoter 256 285
protease cleavage site TEV TEV protease cleavage site 382 402
selectable marker CmR chloramphenicol resistance gene (CmR) (lost in with-insert form) 502 1158
selectable marker KanR kanamycin resistance gene 2396 3208
tag His N-terminal 6xHis tag 364 381
trxn regulatory element arabinose O1 operator arabinose O1 operator 161 182
trxn regulatory element arabinose O2 operator arabinose O2 operator 5 23
trxn termination sequence rrnB rrnB T2 transcription terminator 2258 2284

 

  • Protein
  • Clone
  • Experimental
  • Reagents

Protein

SMART domain : LysM : Lysin motif
Structural Annotation Wiki : TOPSAN
UniProt : Q12RC9

Clone

Cloning Information : 525071
HIP Master Clone ID : 435391
Original Clone ID : 49083

Experimental

TargetTrack - Experimental data : 396440

Reagents

Labome : Sden_0707-cDNA