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RAMP3 (Homo sapiens) in pVP16 (His and MBP-tagged bacterial expression vector)

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Explanation of Terms


Gene: Gene Symbol:  RAMP3
Gene Name:  receptor (G protein-coupled) activity modifying protein 3
Sequence             
Original Clone ID: 5902.1.32_GO.91311
Keyword: partial cds
Species: Homo sapiens
Type: cDNA
Vector Name: pVP16               Format:  CLOSED
Source: Center for Eukaryotic Structural Genomics
Description: None
Comments: Parent TargetDB: GO.42451; His8-MBP fusion protein with TEV protease cleavage site is produced; after cleavage the protein obtained will be the translated CDS with the addition of SER at the N-term
Mutation/
Discrepancy :
No / No
Publications: None
Authors: PSI
Center for Eukaryotic Structural Genomics
HsCD00333963

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AVAILABLE
No restriction
Special MTA: PSI

 

Insert sequence: 381nts         Open reading frame : 1 to 381
Reference Sequence Alignment

GAAAACCTGTACTTCCAGTCCGAGACAGGCATGTTGGAGAGGCTGCCCCTGTGTGGGAAG
GCTTTCGCAGACATGATGGGCAAGGTGGACGTCTGGAAGTGGTGCAACCTGTCCGAGTTC
ATCGTGTACTATGAGAGTTTCACCAACTGCACCGAGATGGAGGCCAATGTCGTGGGCTGC
TACTGGCCCAACCCCCTGGCCCAGGGCTTCATCACCGGCATCCACAGGCAGTTCTTCTCC
AACTGCACCGTGGACAGGGTCCACTTGGAGGACCCCCCAGACGAGGTTCTCATCCCGCTG
ATCGTTATACCCGTCGTTCTGACTGTCGCCATGGCTGGCCTGGTGGTGTGGCGCAGCAAA
CGCACCGACACGCTGCTGTGA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
ACAAGTTTGTACAAAAAAGCAGGCTCC

3' Linker Sequence:
TAGGACCCAGCTTTCTTGTACAAAGTGGT

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: ProteinExpressed: Protein_Confirmed
ProteinPurified: Tested_Not_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in GO.91311
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pVP16

pVP16 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  MBP Forward
Reverse:  pQE Reverse
Description: Bacterial (E. coli) expression, adds N-terminal 8xHis and MBP, adds TEV site on 5p end of insert; ampicillin resistance in bacteria; recombinational cloning; Gateway cloning into this vector will remove the Gateway ccdB death casette.
Comments: TEV protease cleavage site is "partial" and not functional. The TEV cleavage site must be added to the insert by PCR, which allows amino acids encoded by the attB1 site to be cut off after protein expression. Gateway cloning into this vector will remove the Gateway cassette
Size (bp): 7631
Parent Vector: None
Empty Vector: EvNO00084279
Properties: Gateway, acceptor (destination), bacterial expression, recombinational cloning, with tag/fusion/marker
Author Name: Center for Eukaryotic Structural Genomics
Publications: PMID: 15750721
Title: Results from high-throughput DNA cloning of Arabidopsis thaliana target genes using site-specific recombination

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori ColE1-type bacterial origin of replication 6471 5789
negative selection marker ccdB ccdB death cassette gene (lost in with-insert form) 2643 2852
promoter T5 T5 promoter 5 54
recombination site AttR1 AttR1 recombination site 1316 1440
recombination site AttR2 AttR2 recombination site 3020 2896
repressor protein gene LacI LacI coding region 4132 5211
selectable marker AmpR ampicillin resistance gene 7228 6569
selectable marker CmR chloramphenicol resistance gene (CmR) (Lost in with-insert form) 1549 2205
tag His N-terminal 8xHis tag 128 145
tag MBP N-terminal MBP 148 1246

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Experimental
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : RAMP3
Symbol Nomenclature : RAMP3
Designation : CRLR activity-modifying protein 3|calcitonin receptor-like receptor activity modifying protein 3|calcitonin-receptor-like receptor activity-modifying protein 3|receptor (calcitonin) activity modifying protein 3|receptor activity modifying protein 3|receptor activity-modifying protein 3|receptor-activity-modifying protein 3
Full Nomenclature : receptor (G protein-coupled) activity modifying protein 3
GENEID : 10268
GI : 5032023
GenBank Accession : NM_005856
HGNC : 9845
MIM : 605155
Vega : OTTHUMG00000023729
Target GenBank: NM_005856

 

Reference Sequence Alignment

  

HsCD00333963      ------------------------------------------------------------
NM_005856.2       ATGGAGACTGGAGCGCTGCGGCGCCCGCAACTTCTCCCGTTGCTGCTGCTGCTCTGCGGT
                                                                              

HsCD00333963      ------GAAAACCTGTACTTCCAGTCCGAGACAGGCATGTTGGAGAGGCTGCCCCTGTGT
NM_005856.2       GGGTGTCCCAGAGCAGGCGGCTGCAACGAGACAGGCATGTTGGAGAGGCTGCCCCTGTGT
                         ..*..  . .*  * . :.**********************************

HsCD00333963      GGGAAGGCTTTCGCAGACATGATGGGCAAGGTGGACGTCTGGAAGTGGTGCAACCTGTCC
NM_005856.2       GGGAAGGCTTTCGCAGACATGATGGGCAAGGTGGACGTCTGGAAGTGGTGCAACCTGTCC
                  ************************************************************

HsCD00333963      GAGTTCATCGTGTACTATGAGAGTTTCACCAACTGCACCGAGATGGAGGCCAATGTCGTG
NM_005856.2       GAGTTCATCGTGTACTATGAGAGTTTCACCAACTGCACCGAGATGGAGGCCAATGTCGTG
                  ************************************************************

HsCD00333963      GGCTGCTACTGGCCCAACCCCCTGGCCCAGGGCTTCATCACCGGCATCCACAGGCAGTTC
NM_005856.2       GGCTGCTACTGGCCCAACCCCCTGGCCCAGGGCTTCATCACCGGCATCCACAGGCAGTTC
                  ************************************************************

HsCD00333963      TTCTCCAACTGCACCGTGGACAGGGTCCACTTGGAGGACCCCCCAGACGAGGTTCTCATC
NM_005856.2       TTCTCCAACTGCACCGTGGACAGGGTCCACTTGGAGGACCCCCCAGACGAGGTTCTCATC
                  ************************************************************

HsCD00333963      CCGCTGATCGTTATACCCGTCGTTCTGACTGTCGCCATGGCTGGCCTGGTGGTGTGGCGC
NM_005856.2       CCGCTGATCGTTATACCCGTCGTTCTGACTGTCGCCATGGCTGGCCTGGTGGTGTGGCGC
                  ************************************************************

HsCD00333963      AGCAAACGCACCGACACGCTGCTGTGA
NM_005856.2       AGCAAACGCACCGACACGCTGCTGTGA
                  ***************************

Pathways

Reactome : Calcitonin-like ligand receptors
Reactome : Class B/2 (Secretin family receptors)
Reactome : G alpha (s) signalling events
Reactome : GPCR downstream signaling
Reactome : GPCR ligand binding
Reactome : Signal Transduction
Reactome : Signaling by GPCR
WikiPathway : Myometrial Relaxation and Contraction Pathways

Protein

Structural Annotation Wiki : TOPSAN
UniProt : A4D2L1
UniProt : O60896
HPRD : 05517

Clone

Cloning Information : 509324
HIP Master Clone ID : 420491
Original Clone ID : 5902.1.32_GO.91311

Experimental

TargetTrack - Experimental data : GO.91311

Organism


TAX_ID : 9606
Species Specific ID: 10268

Genome

Chromosome : 7
Map Location : 7p13-p12
Ensembl : ENSG00000122679

Reagents

Labome : RAMP3-antibody