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UBE2Q2 (Homo sapiens) in pVP16 (His and MBP-tagged bacterial expression vector)

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Explanation of Terms


Gene: Gene Symbol:  UBE2Q2
Gene Name:  ubiquitin-conjugating enzyme E2Q family member 2
Sequence             
Original Clone ID: 5881.1.65_GO.36829
Keyword: full-length cds
Species: Homo sapiens
Type: cDNA
Vector Name: pVP16               Format:  CLOSED
Source: Center for Eukaryotic Structural Genomics
Description: None
Comments: His8-MBP fusion protein with TEV protease cleavage site is produced; after cleavage the protein obtained will be the translated CDS with the addition of SER at the N-term
Mutation/
Discrepancy :
No / No
Publications: None
Authors: PSI
Center for Eukaryotic Structural Genomics
HsCD00334001

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AVAILABLE
No restriction
Special MTA: PSI

 

Insert sequence: 1146nts         Open reading frame : 1 to 1146
Reference Sequence Alignment

GAAAACCTGTACTTCCAGTCCTCCGTGTCAGGGCTCAAGGCCGAGCTGAAGTTCCTGGCG
TCCATCTTCGACAAGAACCACGAGCGATTCCGCATCGTCAGTTGGAAGCTGGACGAGCTG
CACTGCCAGTTCCTGGTGCCGCAGCAGGGCAGCCCGCACTCGCTGCCGCCGCCACTCACG
CTCCACTGCAACATCACGGAATCCTATCCATCTTCTTCACCGATATGGTTTGTGGATTCT
GAAGACCCAAATCTGACATCAGTTCTGGAACGTCTAGAAGATACTAAGAACAACAATTTG
CTTCGTCAGCAATTGAAGTGGTTGATATGTGAACTCTGCAGTTTATATAACCTTCCTAAG
CACCTGGATGTTGAGATGCTAGATCAACCACTACCCACGGGTCAGAATGGGACAACAGAA
GAAGTGACTTCAGAAGAAGAGGAAGAAGAAGAAGAGATGGCTGAAGATATAGAAGACTTA
GATCACTATGAGATGAAGGAAGAAGAGCCTATTAGTGGGAAAAAGTCAGAGGATGAAGGA
ATTGAAAAAGAAAATTTGGCAATATTAGAGAAAATTAGGAAGACTCAAAGGCAAGACCAT
TTAAATGGTGCAGTGTCTGGGTCAGTGCAAGCTTCAGATAGACTTATGAAAGAGCTCAGG
GACATATACAGATCACAGAGTTATAAAACAGGGATTTATTCAGTGGAACTCATAAATGAC
AGTTTATATGACTGGCATGTTAAACTGCAGAAGGTTGACCCTGATAGTCCTTTGCACAGT
GATCTTCAGATCTTAAAAGAAAAAGAAGGCATAGAATATATTTTGCTTAACTTCTCTTTT
AAGGATAACTTTCCATTTGATCCTCCATTTGTTCGAGTGGTGTTACCTGTTCTCTCAGGA
GGGTATGTATTGGGTGGAGGAGCATTATGTATGGAACTTCTCACAAAACAGGGCTGGAGC
AGTGCCTACTCAATAGAATCGGTCATCATGCAAATAAATGCCACCTTAGTCAAAGGCAAA
GCCAGAGTGCAGTTTGGAGCAAATAAGAATCAATATAATCTAGCAAGAGCCCAACAATCC
TATAATTCCATTGTACAGATACATGAGAAAAATGGCTGGTACACCCCTCCAAAGGAAGAT
GGCTAA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
ACAAGTTTGTACAAAAAAGCAGGCTCC

3' Linker Sequence:
TAGGACCCAGCTTTCTTGTACAAAGTGGT

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: ProteinExpressed: Not_Applicable
ProteinPurified: Not_Applicable
SolubleProtein: Not_Applicable
Find experimental protocols in GO.36829
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pVP16

pVP16 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  MBP Forward
Reverse:  pQE Reverse
Description: Bacterial (E. coli) expression, adds N-terminal 8xHis and MBP, adds TEV site on 5p end of insert; ampicillin resistance in bacteria; recombinational cloning; Gateway cloning into this vector will remove the Gateway ccdB death casette.
Comments: TEV protease cleavage site is "partial" and not functional. The TEV cleavage site must be added to the insert by PCR, which allows amino acids encoded by the attB1 site to be cut off after protein expression. Gateway cloning into this vector will remove the Gateway cassette
Size (bp): 7631
Parent Vector: None
Empty Vector: EvNO00084279
Properties: Gateway, acceptor (destination), bacterial expression, recombinational cloning, with tag/fusion/marker
Author Name: Center for Eukaryotic Structural Genomics
Publications: PMID: 15750721
Title: Results from high-throughput DNA cloning of Arabidopsis thaliana target genes using site-specific recombination

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori ColE1-type bacterial origin of replication 6471 5789
negative selection marker ccdB ccdB death cassette gene (lost in with-insert form) 2643 2852
promoter T5 T5 promoter 5 54
recombination site AttR1 AttR1 recombination site 1316 1440
recombination site AttR2 AttR2 recombination site 3020 2896
repressor protein gene LacI LacI coding region 4132 5211
selectable marker AmpR ampicillin resistance gene 7228 6569
selectable marker CmR chloramphenicol resistance gene (CmR) (Lost in with-insert form) 1549 2205
tag His N-terminal 8xHis tag 128 145
tag MBP N-terminal MBP 148 1246

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Experimental
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : UBE2Q2
Symbol Nomenclature : UBE2Q2
Designation : ubiquitin carrier protein Q2|ubiquitin-conjugating enzyme E2 Q2|ubiquitin-protein ligase Q2
Full Nomenclature : ubiquitin-conjugating enzyme E2Q family member 2
GENEID : 92912
GI : 50949459
GenBank Accession : AL832429
HGNC : 19248
MIM : 612501
Vega : OTTHUMG00000142839
Target GenBank: AL832429

 

Reference Sequence Alignment

  

HsCD00334001        GAAAACCTGTACTTCCAGTCCTCCGTGTCAGGGCTCAAGGCCGAGCTGAAGTTCCTGGCG
NM_173469.3         ------------------ATGTCCGTGTCAGGGCTCAAGGCCGAGCTGAAGTTCCTGGCG
NM_001145335.1      ------------------------------------------------------ATGAGA
                                                                          .**. .

HsCD00334001        TCCATCTTCGACAAGAACCACGAGCGATTCCGCATCGTCAGTTGGAAGCTGGACGAGCTG
NM_173469.3         TCCATCTTCGACAAGAACCACGAGCGATTCCGCATCGTCAGTTGGAAGCTGGACGAGCTG
NM_001145335.1      ATGGACTCGTTGACGGAGGAAAAGTTGGAATGCAGACTCTGGTGCTGTTTGAGCGACCCC
                    :  .:**   : *.*.*  *..**  . :. *** . **:* ** :.  **..*** *  

HsCD00334001        CACTGCCAGTTCCTGGTGCCGCAGCAGGGCAGCCCGCACTCGCTGCCGCCGCCACTCACG
NM_173469.3         CACTGCCAGTTCCTGGTGCCGCAGCAGGGCAGCCCGCACTCGCTGCCGCCGCCACTCACG
NM_001145335.1      TCTCCCCCGGGCCTGGCTGCGCGCTG------------CTGTGTTCTGGAAAGGAGCATA
                     .   **.*  *****   ***.  .            **   * * * ... .. ** .

HsCD00334001        CTCCACTGCAACATCACGGAATCCTATCCATCTTCTTCACCGATATGGTTTGTGGATTCT
NM_173469.3         CTCCACTGCAACATCACGGAATCCTATCCATCTTCTTCACCGATATGGTTTGTGGATTCT
NM_001145335.1      GTACCGTCGTTGCGGCAGGAATCCTATCCATCTTCTTCACCGATATGGTTTGTGGATTCT
                     *.*. *  :: .  ..*******************************************

HsCD00334001        GAAGACCCAAATCTGACATCAGTTCTGGAACGTCTAGAAGATACTAAGAACAACAATTTG
NM_173469.3         GAAGACCCAAATCTGACATCAGTTCTGGAACGTCTAGAAGATACTAAGAACAACAATTTG
NM_001145335.1      GAAGACCCAAATCTGACATCAGTTCTGGAACGTCTAGAAGATACTAAGAACAACAATTTG
                    ************************************************************

HsCD00334001        CTTCGTCAGCAATTGAAGTGGTTGATATGTGAACTCTGCAGTTTATATAACCTTCCTAAG
NM_173469.3         CTTCGTCAGCAATTGAAGTGGTTGATATGTGAACTCTGCAGTTTATATAACCTTCCTAAG
NM_001145335.1      CTTCGTCAGCAATTGAAGTGGTTGATATGTGAACTCTGCAGTTTATATAACCTTCCTAAG
                    ************************************************************

HsCD00334001        CACCTGGATGTTGAGATGCTAGATCAACCACTACCCACGGGTCAGAATGGGACAACAGAA
NM_173469.3         CACCTGGATGTTGAGATGCTAGATCAACCACTACCCACGGGTCAGAATGGGACAACAGAA
NM_001145335.1      CACCTGGATGTTGAGATGCTAGATCAACCACTACCCACGGGTCAGAATGGGACAACAGAA
                    ************************************************************

HsCD00334001        GAAGTGACTTCAGAAGAAGAGGAAGAAGAAGAAGAGATGGCTGAAGATATAGAAGACTTA
NM_173469.3         GAAGTGACTTCAGAAGAAGAGGAAGAAGAAGAAGAGATGGCTGAAGATATAGAAGACTTA
NM_001145335.1      GAAGTGACTTCAGAAGAAGAGGAAGAAGAAGAAGAGATGGCTGAAGATATAGAAGACTTA
                    ************************************************************

HsCD00334001        GATCACTATGAGATGAAGGAAGAAGAGCCTATTAGTGGGAAAAAGTCAGAGGATGAAGGA
NM_173469.3         GATCACTATGAGATGAAGGAAGAAGAGCCTATTAGTGGGAAAAAGTCAGAGGATGAAGGA
NM_001145335.1      GATCACTATGAGATGAAGGAAGAAGAGCCTATTAGTGGGAAAAAGTCAGAGGATGAAGGA
                    ************************************************************

HsCD00334001        ATTGAAAAAGAAAATTTGGCAATATTAGAGAAAATTAGGAAGACTCAAAGGCAAGACCAT
NM_173469.3         ATTGAAAAAGAAAATTTGGCAATATTAGAGAAAATTAGGAAGACTCAAAGGCAAGACCAT
NM_001145335.1      ATTGAAAAAGAAAATTTGGCAATATTAGAGAAAATTAGGAAGACTCAAAGGCAAGACCAT
                    ************************************************************

HsCD00334001        TTAAATGGTGCAGTGTCTGGGTCAGTGCAAGCTTCAGATAGACTTATGAAAGAGCTCAGG
NM_173469.3         TTAAATGGTGCAGTGTCTGGGTCAGTGCAAGCTTCAGATAGACTTATGAAAGAGCTCAGG
NM_001145335.1      TTAAATGGTGCAGTGTCTGGGTCAGTGCAAGCTTCAGATAGACTTATGAAAGAGCTCAGG
                    ************************************************************

HsCD00334001        GACATATACAGATCACAGAGTTATAAAACAGGGATTTATTCAGTGGAACTCATAAATGAC
NM_173469.3         GACATATACAGATCACAGAGTTATAAAACAGGGATTTATTCAGTGGAACTCATAAATGAC
NM_001145335.1      GACATATACAGATCACAGAGTTATAAAACAGGGATTTATTCAGTGGAACTCATAAATGAC
                    ************************************************************

HsCD00334001        AGTTTATATGACTGGCATGTTAAACTGCAGAAGGTTGACCCTGATAGTCCTTTGCACAGT
NM_173469.3         AGTTTATATGACTGGCATGTTAAACTGCAGAAGGTTGACCCTGATAGTCCTTTGCACAGT
NM_001145335.1      AGTTTATATGACTGGCATGTTAAACTGCAGAAGGTTGACCCTGATAGTCCTTTGCACAGT
                    ************************************************************

HsCD00334001        GATCTTCAGATCTTAAAAGAAAAAGAAGGCATAGAATATATTTTGCTTAACTTCTCTTTT
NM_173469.3         GATCTTCAGATCTTAAAAGAAAAAGAAGGCATAGAATATATTTTGCTTAACTTCTCTTTT
NM_001145335.1      GATCTTCAGATCTTAAAAGAAAAAGAAGGCATAGAATATATTTTGCTTAACTTCTCTTTT
                    ************************************************************

HsCD00334001        AAGGATAACTTTCCATTTGATCCTCCATTTGTTCGAGTGGTGTTACCTGTTCTCTCAGGA
NM_173469.3         AAGGATAACTTTCCATTTGATCCTCCATTTGTTCGAGTGGTGTTACCTGTTCTCTCAGGA
NM_001145335.1      AAGGATAACTTTCCATTTGATCCTCCATTTGTTCGAGTGGTGTTACCTGTTCTCTCAGGA
                    ************************************************************

HsCD00334001        GGGTATGTATTGGGTGGAGGAGCATTATGTATGGAACTTCTCACAAAACAGGGCTGGAGC
NM_173469.3         GGGTATGTATTGGGTGGAGGAGCATTATGTATGGAACTTCTCACAAAACAGGGCTGGAGC
NM_001145335.1      GGGTATGTATTGGGTGGAGGAGCATTATGTATGGAACTTCTCACAAAACAGGGCTGGAGC
                    ************************************************************

HsCD00334001        AGTGCCTACTCAATAGAATCGGTCATCATGCAAATAAATGCCACCTTAGTCAAAGGCAAA
NM_173469.3         AGTGCCTACTCAATAGAATCGGTCATCATGCAAATAAATGCCACCTTAGTCAAAGGCAAA
NM_001145335.1      AGTGCCTACTCAATAGAATCGGTCATCATGCAAATAAATGCCACCTTAGTCAAAGGCAAA
                    ************************************************************

HsCD00334001        GCCAGAGTGCAGTTTGGAGCAAATAAGAATCAATATAATCTAGCAAGAGCCCAACAATCC
NM_173469.3         GCCAGAGTGCAGTTTGGAGCAAATAAGAATCAATATAATCTAGCAAGAGCCCAACAATCC
NM_001145335.1      GCCAGAGTGCAGTTTGGAGCAAATAAGAATCAATATAATCTAGCAAGAGCCCAACAATCC
                    ************************************************************

HsCD00334001        TATAATTCCATTGTACAGATACATGAGAAAAATGGCTGGTACACCCCTCCAAAGGAAGAT
NM_173469.3         TATAATTCCATTGTACAGATACATGAGAAAAATGGCTGGTACACCCCTCCAAAGGAAGAT
NM_001145335.1      TATAATTCCATTGTACAGATACATGAGAAAAATGGCTGGTACACCCCTCCAAAGGAAGAT
                    ************************************************************

HsCD00334001        GGCTAA
NM_173469.3         GGCTAA
NM_001145335.1      GGCTAA
                    ******

Pathways

Reactome : Adaptive Immune System
Reactome : Antigen processing: Ubiquitination & Proteasome degradation
Reactome : Class I MHC mediated antigen processing & presentation
Reactome : Immune System

Protein

SMART domain : UBCc : Ubiquitin-conjugating enzyme E2, catalytic domain homologues
Structural Annotation Wiki : TOPSAN
UniProt : Q8WVN8
UniProt : E9PHD0
UniProt : B7Z3Q2
HPRD : 14294

Clone

Cloning Information : 509399
HIP Master Clone ID : 420566
Original Clone ID : 5881.1.65_GO.36829

Experimental

TargetTrack - Experimental data : GO.36829

Organism


TAX_ID : 9606
Species Specific ID: 92912

Genome

Chromosome : 15
Map Location : 15q24.2
Ensembl : ENSG00000140367

Reagents

Labome : UBE2Q2-antibody