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Gmet_3143 (Geobacter metallireducens GS-15) in pSGX3 (His-tagged bacterial expression vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  Gmet_3143
Gene Name:  resolvase-like serine recombinase
Sequence             
Original Clone ID: 11221f1BCt4p1
Keyword: None
Species: Geobacter metallireducens GS-15
Type: cDNA
Vector Name: pSGX3               Format:  CLOSED
Source: New York SGX Research Center for Structural Genomics
Description: None
Comments: C-terminal 6-His, TOPO
Mutation/
Discrepancy :
No / No
Publications: None
Authors: PSI
New York SGX Research Center for Structural Genomics
GmCD00335219

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AVAILABLE
No restriction
Special MTA: PSI

 

Insert sequence: 414nts         Open reading frame : 1 to 414

GAACGACCTCTTTGTATTTCGTATATCCGGTTTTCAAAAGCAACACAAGCAGAGGGAAGC
AGCTTGGAAAGACAGCTTCAACTCTCCCGAACGTACGCAGAAGAACACGGGTTAGTGCTA
GATGATAAACATTCCTATCAAGATTTGGGTAAGTCCGCCTTCACGAGTGAACATGTCAAG
GAAGGTGAATTTGGTGTATTTCTCGAATTTGTGAAGCAAGGACGGGTGCCTAAAGGATCG
GTTCTTCTAGTCGAATCGCTGGACCGATTGAGTCGAGAGAGTGTATTGACAGCCTTCAAG
CAATTTCAAGAGATTTTGGACGCTGGCATCAAAATCGTTACTCTTAGTGACAAAATGGAG
TACACAAAAGAAGGATTTGAAGCGAATGAAGGGCATCATCACCATCACCACTGA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
ATGTCCCTT

3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: ProteinExpressed: Protein_Confirmed
ProteinPurified: Tested_Not_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in NYSGXRC-11221f
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pSGX3

pSGX3 in advanced viewed

Synonyms: pSGX-3, pSGX3_BC
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial (E. coli) expression vector with T7 promoter, adds C-terminal 6xHis tag; kanamycin resistance; TOPO cloning.
Comments: Modified from pET26b from Novagen
Size (bp): 5339
Parent Vector: None
Empty Vector: None
Properties: TOPO Cloning, bacterial expression, in vitro transcription, with tag/fusion/marker
Author Name: New York SGX Research Center for Structural Genomics
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
ATG start start initiation start (Met, Ser, Leu) 5072 5079
TOPO recognition site TOPO TOPO cloning site 5076 5085
bacterial origin ori pBR322-type bacterial origin of replication 1421 2103
gene fragment ROP ROP 2527 2703
promoter T7 T7 promoter 4983 5003
repressor protein gene LacI LacI CDS 3515 4531
selectable marker KanR kanamycin resistance gene 563 1375
tag His 6xHis tag 5086 5103
trxn regulatory element lac operator lac operator 5003 5025
trxn termination sequence T7 term T7 terminator 5231 5307

 

  • Gene
  • Protein
  • Clone
  • Experimental
  • Organism
  • Reagents

Gene

 

Gene Symbol : Gmet_3143
GENEID : 3740917
Locus Tag : Gmet_3143
GI : 78195589
GenBank Accession : ABB33356.1
Target GenBank: None

 

Protein

SMART domain : Resolvase : Resolvase, N terminal domain
Structural Annotation Wiki : TOPSAN
UniProt : Q39QW8

Clone

Cloning Information : 550970
HIP Master Clone ID : 461290
Original Clone ID : 11221f1BCt4p1

Experimental

TargetTrack - Experimental data : NYSGXRC-11221f

Organism


TAX_ID : 269799
Species Specific ID: 3740917

Reagents

Labome : Gmet_3143-cDNA