DNASU Plasmid Repository • 480.965.5697 | Email

Detailed Vector Information: ZM552

TOP

Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: ZM552              
Source: Zarmik Moqtaderi
Description: Yeast expression vector with N-terminal 3X V5 epitope tag; ampicillin resistance in bacteria; LEU2 resistance in yeast; Gateway cloning.
Comments: None
Publications: None
Authors: Zarmik Moqtaderi
EvNO00335423

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  yeast    Marker:  LEU2
       DyNA Vector Map

         Powered by LabGenius

Vector Name: ZM552

ZM552 in advanced viewed

Synonyms: ZM552
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Yeast expression vector with N-terminal 3X V5 epitope tag; ampicillin resistance in bacteria; LEU2 resistance in yeast; Gateway cloning.
Comments: ZM511 Stu1 vector plus Gateway attR frame B cassette EcoRV fragment; same as ZM515. Cloning into the vector removes the ccdB cassette and chloramphenicol resistance.
Size (bp): 8302
Parent Vector: None
Properties: Gateway, acceptor (destination), recombinational cloning, with tag/fusion/marker, yeast expression
Author Name: Zarmik Moqtaderi
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
primer site M13R M13 reverse sequencing primer 2630 2647
primer site M13F M13 forward sequencing primer 8286 8301
promoter CMD1 CMD1 promoter 295 484
recombination site attR1 attR1 recombinational cloning site 650 666
recombination site attR2 attR2 recombination site 2337 2352
reporter gene LacZ LacZ alpha gene 8146 8214
selectable marker CmR chloramphenicol resistance. removed with the ccdB cassette in clones with insert 883 1539
selectable marker LEU2 LEU2 selectable marker in yeast 5273 5344
selectable marker AmpR ampcillin resistance 3795 4454
tag V5 N-terminal triple V5 tag 494 631
trxn termination sequence CYC1 CYC1 terminator 2377 2582
yeast ARS ARS ARS yeast origin of replication 0 0
yeast centromere CEN yeast centromere 0 0

 

Species Specific ID: None
Target GenBank: None