MMP0046 (M. maripaludis S2 )
In pSpeedET (bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  MMP0046
Gene Name:  hypothetical protein
Original Clone ID:
34161
Keyword:
full-length cds
Species:
Methanococcus maripaludis S2
Type:
cDNA
Vector Name:
pSpeedET
Format: 
CLOSED
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
Yes / Yes
Publications:
None
Authors:
PSI
Joint Center for Structural Genomics
MmCD00336099
Price: 
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 453nts
Open reading frame: 1 to 453

ATGAAAATTCCTAAAATTTACGTTGAAGGAGAATTAAACGACGGCGATCGTGTCGCAATT
GAAAAAGATGGAAATGCAATAATTTTTTTAGAAAAAGATGAAGAATATTCTGGAAACGGC
AAGCTTCTTTATCAGGTAATATATGATGATTTAGCAAAATACATGAGTTTAGACACTTTA
AAAAAAGATGTTTTAATACAGTATCCTGATAAACATACATTTACATATCTTAAAGCAGGA
ACTAAACTTATTTCGGTACCTGCTGAGGGCTACAAAGTCTATCCAATAATGGATTTTGGT
TTCAGGGTTTTAAAAGGATACAGGCTTGCAACACTTGAAAGTAAAAAGGGAGATTTAAGG
TACGTTAATTCTCCAGTAAGCGGAACGGTCATATTTATGAACGAAATCCCATCAGAAAGA
GCAAATTATGTATTTTACATGCTCGAAGAATAA

Protein sequence predicted based on nucleotide sequence

MKIPKIYVEGELNDGDRVAIEKDGNAIIFLEKDEEYSGNGKLLYQVIYDDLAKYMSLDTL
KKDVLIQYPDKHTFTYLKAGTKLISVPAEGYKVYPIMDFGFRVLKGYRLATLESKKGDLR
YVNSPVSGTVIFMNEIPSERANYVFYMLEE*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
F74L

Discrepancy:
g312a,L104L;t363c,Y121Y;
t381c,S127S;t408c,I136I;
g411a,P137P
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in 381816
Recommended expression in:
Not Applicable