DNASU Plasmid | MRE11A (H. sapiens )<br>In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: MRE11A

Gene Name: MRE11 meiotic recombination 11 homolog A (S. cerevisiae)

Original Clone ID:

8658a8BCt5p1

Keyword:

full-length cds

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pSGX3

Format:

CLOSED

Source:

New York SGX Research Center for Structural Genomics

Description:

None

Comments:

C-terminal 6-His, TOPO

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

PSI
New York SGX Research Center for Structural Genomics

HsCD00336657

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 1071nts

Open reading frame: 1 to 1071

Reference Sequence Alignment

AGTACTGCAGATGCACTTGATGATGAAAACACATTTAAAATATTAGTTGCAACAGATATT
CATCTTGGATTTATGGAGAAAGATGCAGTCAGAGGAAATGATACGTTTGTAACACTCGAT
GAAATTTTAAGACTTGCCCAGGAAAATGAAGTGGATTTTATTTTGTTAGGTGGTGATCTT
TTTCATGAAAATAAGCCCTCAAGGAAAACATTACATACCTGCCTCGAGTTATTAAGAAAA
TATTGTATGGGTGATCGGCCTGTCCAGTTTGAAATTCTCAGTGATCAGTCAGTCAACTTT
GGTTTTAGTAAGTTTCCATGGGTGAACTATCAAGATGGCAACCTCAACATTTCAATTCCA
GTGTTTAGTATTCATGGCAATCATGACGATCCCACAGGGGCAGATGCACTTTGTGCCTTG
GACATTTTAAGTTGTGCTGGATTTGTAAATCACTTTGGACGTTCAATGTCTGTGGAGAAG
ATAGACATTAGTCCGGTTTTGCTTCAAAAAGGAAGCACAAAGATTGCGCTATATGGTTTA
GGATCCATTCCAGATGAAAGGCTCTATCGAATGTTTGTCAATAAAAAAGTAACAATGTTG
AGACCAAAGGAAGATGAGAACTCTTGGTTTAACTTATTTGTGATTCATCAGAACAGGAGT
AAACATGGAAGTACTAACTTCATTCCAGAACAATTTTTGGATGACTTCATTGATCTTGTT
ATCTGGGGCCATGAACATGAGTGTAAAATAGCTCCAACCAAAAATGAACAACAGCTGTTT
TATATCTCACAACCTGGAAGCTCAGTGGTTACTTCTCTTTCCCCAGGAGAAGCTGTAAAG
AAACATGTTGGTTTGCTGCGTATTAAAGGGAGGAAGATGAATATGCATAAAATTCCTCTT
CACACAGTGCGGCAGTTTTTCATGGAGGATATTGTTCTAGCTAATCATCCAGACATTTTT
AACCCAGATAATCCTAAAGTAACCCAAGCCATACAAAGCTTCTGTTTGGAGAAGATTGAA
GAAATGCTTGAAAATGCTGAACGGGAAGGGCATCATCACCATCACCACTGA

Protein sequence predicted based on nucleotide sequence

STADALDDENTFKILVATDIHLGFMEKDAVRGNDTFVTLDEILRLAQENEVDFILLGGDL
FHENKPSRKTLHTCLELLRKYCMGDRPVQFEILSDQSVNFGFSKFPWVNYQDGNLNISIP
VFSIHGNHDDPTGADALCALDILSCAGFVNHFGRSMSVEKIDISPVLLQKGSTKIALYGL
GSIPDERLYRMFVNKKVTMLRPKEDENSWFNLFVIHQNRSKHGSTNFIPEQFLDDFIDLV
IWGHEHECKIAPTKNEQQLFYISQPGSSVVTSLSPGEAVKKHVGLLRIKGRKMNMHKIPL
HTVRQFFMEDIVLANHPDIFNPDNPKVTQAIQSFCLEKIEEMLENAEREGHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: kanamycin

Bacterial Selection Condition: 50ug/mL kanamycin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.

Protein Expression Results:

ProteinExpressed: Protein_Confirmed

SolubleProtein: Protein_Soluble

ProteinPurified: Tested_Not_Purified

Find experimental protocols in NYSGXRC-8658a

Recommended expression in:

Not Applicable

Vector Name:

pSGX3

Synonyms:

pSGX-3, pSGX3_BC

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Bacterial (E. coli) expression vector with T7 promoter, adds C-terminal 6xHis tag; kanamycin resistance; TOPO cloning.

Comments:

Modified from pET26b from Novagen

Size (bp):

5339

Parent Vector:

None

Properties:

TOPO Cloning, bacterial expression, in vitro transcription, with tag/fusion/marker

Author Name:

New York SGX Research Center for Structural Genomics

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

TGGCGAATGGGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGTTTACAATTTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAATTAATTCTTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGTTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGACGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTAGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCTGCGGTAAAGCTCATCAGCGTGGTCGTGAAGCGATTCACAGATGTCTGCCTGTTCATCCGCGTCCAGCTCGTTGAGTTTCTCCAGAAGCGTTAATGTCTGGCTTCTGATAAAGCGGGCCATGTTAAGGGCGGTTTTTTCCTGTTTGGTCACTGATGCCTCCGTGTAAGGGGGATTTCTGTTCATGGGGGTAATGATACCGATGAAACGAGAGAGGATGCTCACGATACGGGTTACTGATGATGAACATGCCCGGTTACTGGAACGTTGTGAGGGTAAACAACTGGCGGTATGGATGCGGCGGGACCAGAGAAAAATCACTCAGGGTCAATGCCAGCGCTTCGTTAATACAGATGTAGGTGTTCCACAGGGTAGCCAGCAGCATCCTGCGATGCAGATCCGGAACATAATGGTGCAGGGCGCTGACTTCCGCGTTTCCAGACTTTACGAAACACGGAAACCGAAGACCATTCATGTTGTTGCTCAGGTCGCAGACGTTTTGCAGCAGCAGTCGCTTCACGTTCGCTCGCGTATCGGTGATTCATTCTGCTAACCAGTAAGGCAACCCCGCCAGCCTAGCCGGGTCCTCAACGACAGGAGCACGATCATGCGCACCCGTGGGGCCGCCATGCCGGCGATAATGGCCTGCTTCTCGCCGAAACGTTTGGTGGCGGGACCAGTGACGAAGGCTTGAGCGAGGGCGTGCAAGATTCCGAATACCGCAAGCGACAGGCCGATCATCGTCGCGCTCCAGCGAAAGCGGTCCTCGCCGAAAATGACCCAGAGCGCTGCCGGCACCTGTCCTACGAGTTGCATGATAAAGAAGACAGTCATAAGTGCGGCGACGATAGTCATGCCCCGCGCCCACCGGAAGGAGCTGACTGGGTTGAAGGCTCTCAAGGGCATCGGTCGAGATCCCGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATATCCGCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCCCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGAATTTGATTGCGAGTGAGATATTTATGCCAGCCAGCCAGACGCAGACGCGCCGAGACAGAACTTAATGGGCCCGCTAACAGCGCGATTTGCTGGTGACCCAATGCGACCAGATGCTCCACGCCCAGTCGCGTACCGTCTTCATGGGAGAAAATAATACTGTTGATGGGTGTCTGGTCAGAGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCCGGGATCTCGACGCTCTCCCTTATGCGACTCCTGCATTAGGAAGCAGCCCAGTAGTAGGTTGAGGCCGTTGAGCACCGCCGCCGCAAGGAATGGTGCATGCAAGGAGATGGCGCCCAACAGTCCCCCGGCCACGGGGCCTGCCACCATACCCACGCCGAAACAAGCGCTCATGAGCCCGAAGTGGCGAGCCCGATCTTCCCCATCGGTGATGTCGGCGATATAGGCGCCAGCAACCGCACCTGTGGCGCCGGTGATGCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGTCCCTTAAGGGCATCATCACCATCACCACTGATCCCACCACCACCACCACCACTGAGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGCACCACCACCACCACCACTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGAT

Vector Features:

Type

Name

Description

Start

End

ATG start

start

initiation start (Met, Ser, Leu)

5072

5079

TOPO recognition site

TOPO

TOPO cloning site

5076

5085

bacterial origin

ori

pBR322-type bacterial origin of replication

1421

2103

gene fragment

ROP

2527

2703

promoter

T7 promoter

4983

5003

repressor protein gene

LacI

LacI CDS

3515

4531

selectable marker

KanR

kanamycin resistance gene

563

1375

tag

His

6xHis tag

5086

5103

trxn regulatory element

lac operator

5003

5025

trxn termination sequence

T7 term

T7 terminator

5231

5307

Gene

Gene Symbol :

MRE11A

Symbol Nomenclature :

MRE11A

SYNONYM :

ATLD; HNGS1; MRE11; MRE11B

Designation :

Full Nomenclature :

MRE11 meiotic recombination 11 homolog A (S. cerevisiae)

GENEID :

4361

GI :

24234690

GenBank Accession :

NM_005590

HGNC :

7230

MIM :

600814

Vega :

OTTHUMG00000167780

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00336657        ------------------------------------------------------------
XM_005274008.1      ------------------------------------------------------------
NM_005591.3         ATGAGTACTGCAGATGCACTTGATGATGAAAACACATTTAAAATATTAGTTGCAACAGAT
NM_005590.3         ------------------------------------------------------------
XM_005274007.1      ------------------------------------------------------------
                                                                                

HsCD00336657        ------------------------------------------------------------
XM_005274008.1      ------------------------------------------------------------
NM_005591.3         ATTCATCTTGGATTTATGGAGAAAGATGCAGTCAGAGGAAATGATACGTTTGTAACACTC
NM_005590.3         ------------------------------------------------------------
XM_005274007.1      ------------------------------------------------------------
                                                                                

HsCD00336657        ------------------------------------------------------------
XM_005274008.1      ------------------------------------------------------------
NM_005591.3         GATGAAATTTTAAGACTTGCCCAGGAAAATGAAGTGGATTTTATTTTGTTAGGTGGTGAT
NM_005590.3         ------------------------------------------------------------
XM_005274007.1      ------------------------------------------------------------
                                                                                

HsCD00336657        ------------------------------------------------------------
XM_005274008.1      ------------------------------------------------------------
NM_005591.3         CTTTTTCATGAAAATAAGCCCTCAAGGAAAACATTACATACCTGCCTCGAGTTATTAAGA
NM_005590.3         ------------------------------------------------------------
XM_005274007.1      ------------------------------------------------------------
                                                                                

HsCD00336657        ------------------------------------------------------------
XM_005274008.1      ------------------------------------------------------------
NM_005591.3         AAATATTGTATGGGTGATCGGCCTGTCCAGTTTGAAATTCTCAGTGATCAGTCAGTCAAC
NM_005590.3         ------------------------------------------------------------
XM_005274007.1      ------------------------------------------------------------
                                                                                

HsCD00336657        ------------------------------------------------------------
XM_005274008.1      ------------------------------------------------------------
NM_005591.3         TTTGGTTTTAGTAAGTTTCCATGGGTGAACTATCAAGATGGCAACCTCAACATTTCAATT
NM_005590.3         ------------------------------------------------------------
XM_005274007.1      ------------------------------------------------------------
                                                                                

HsCD00336657        ------------------------------------------------------------
XM_005274008.1      ------------------------------------------------------------
NM_005591.3         CCAGTGTTTAGTATTCATGGCAATCATGACGATCCCACAGGGGCAGATGCACTTTGTGCC
NM_005590.3         ------------------------------------------------------------
XM_005274007.1      ------------------------------------------------------------
                                                                                

HsCD00336657        ------------------------------------------------------------
XM_005274008.1      ------------------------------------------------ATGTCTGTGGAG
NM_005591.3         TTGGACATTTTAAGTTGTGCTGGATTTGTAAATCACTTTGGACGTTCAATGTCTGTGGAG
NM_005590.3         ------------------------------------------------------------
XM_005274007.1      ------------------------------------------------------------
                                                                                

HsCD00336657        ------------------------------------AGTACTGCAGATGCACTTGATGAT
XM_005274008.1      AAGATAGACATTAGTCCGGTTTTGCTTCAAAAAGGAAGCACAAAGATTGCGCTATATGGT
NM_005591.3         AAGATAGACATTAGTCCGGTTTTGCTTCAAAAAGGAAGCACAAAGATTGCGCTATATGGT
NM_005590.3         ---------------------------------ATGAGTACTGCAGATGCACTTGATGAT
XM_005274007.1      ---------------------------------ATGAGTACTGCAGATGCACTTGATGAT
                                                        ** **:....:***.**: ***.*

HsCD00336657        GAAAACACATTTAAAATATTAGTTGCAACAGATATTCATCTTGGATTTATGGAGAAAGAT
XM_005274008.1      TTAGGATCCATTCCAGATGAAAGGCTCTATCGAATGTTTGTCAATAAAAAAGTAACAATG
NM_005591.3         TTAGGATCCATTCCAGATGAAAGGCTCTATCGAATGTTTGTCAATAAAAAAGTAACAATG
NM_005590.3         GAAAACACATTTAAAATATTAGTTGCAACAGATATTCATCTTGGATTTATGGAGAAAGAT
XM_005274007.1      GAAAACACATTTAAAATATTAGTTGCAACAGATATTCATCTTGGATTTATGGAGAAAGAT
                     :*...:*.:**..*.:: :*.    .:.: .:**  :* * ..::::*:.*:.*.*.: 

HsCD00336657        GCAGTCAGAGGAAATGATACGTTTGTAACACTCGATGAAATTTTAAGACTTGCCCAGGAA
XM_005274008.1      TTGAGACCAAAGGAAGATGAGAACTCTTGGTTTAACTTATTTGTGATTCATCAGAACAGG
NM_005591.3         TTGAGACCAAAGGAAGATGAGAACTCTTGGTTTAACTTATTTGTGATTCATCAGAACAGG
NM_005590.3         GCAGTCAGAGGAAATGATACGTTTGTAACACTCGATGAAATTTTAAGACTTGCCCAGGAA
XM_005274007.1      GCAGTCAGAGGAAATGATACGTTTGTAACACTCGATGAAATTTTAAGACTTGCCCAGGAA
                      .. .. *....*:***..*::   :: . * .*  :*:** *.* :*:* . .* ...

HsCD00336657        AATGAAGTGGATTTTATTTTGTTAGGTGGTGATCTTTTTCATGAAAATAAGCCCTCAAGG
XM_005274008.1      AGTAAACATGGAAGTACTAACTTCATTCCAGAACAATTTTTGGATGACTTCATTGATCTT
NM_005591.3         AGTAAACATGGAAGTACTAACTTCATTCCAGAACAATTTTTGGATGACTTCATTGATCTT
NM_005590.3         AATGAAGTGGATTTTATTTTGTTAGGTGGTGATCTTTTTCATGAAAATAAGCCCTCAAGG
XM_005274007.1      AATGAAGTGGATTTTATTTTGTTAGGTGGTGATCTTTTTCATGAAAATAAGCCCTCAAGG
                    *.*.** : *.:: ** *:: **.. *  :**:*::*** : **:.* :: .   .:.  

HsCD00336657        AAAACATTACATACCTGCCTCGAGTTATTAAGAAAATATTGTATGGGTGATCGGCCTGTC
XM_005274008.1      GTTATCTGGGGCCATGAACATGAGTGTAAAATAGCTCCAACCAAAAATGAACAACAGCTG
NM_005591.3         GTTATCTGGGGCCATGAACATGAGTGTAAAATAGCTCCAACCAAAAATGAACAACAGCTG
NM_005590.3         AAAACATTACATACCTGCCTCGAGTTATTAAGAAAATATTGTATGGGTGATCGGCCTGTC
XM_005274007.1      AAAACATTACATACCTGCCTCGAGTTATTAAGAAAATATTGTATGGGTGATCGGCCTGTC
                    .::* .* . . ..  ..*: **** :::** *..: .::  *:...***:*..*.  * 

HsCD00336657        CAGTTTGAAATTCTCAGTGATCAGTCAGTCAACTTTGGTTTTAGTAAGTTTCCATGGGTG
XM_005274008.1      TTTTATATCTCACAACCTGGAAGCTCAGTGGTTACTTCTCTTTCCCCAGGAGAAGCTGTA
NM_005591.3         TTTTATATCTCACAACCTGGAAGCTCAGTGGTTACTTCTCTTTCCCCAGGAGAAGCTGTA
NM_005590.3         CAGTTTGAAATTCTCAGTGATCAGTCAGTCAACTTTGGTTTTAGTAAGTTTCCATGGGTG
XM_005274007.1      CAGTTTGAAATTCTCAGTGATCAGTCAGTCAACTTTGGTTTTAGTAAGTTTCCATGGGTG
                     : *:*.:.: :*:.. **.:.. ***** .: : *  * **:  ...  : .*   **.

HsCD00336657        AACTATCAAGATGGCAACCTCAACATTTCAATTCCAGTGTTTAGTATTCATGGCAATCAT
XM_005274008.1      AAGAAACATGTTGGTTTGCTGCGTATTAAAGGGAGGAAGATGAATATGCATAAAATTCCT
NM_005591.3         AAGAAACATGTTGGTTTGCTGCGTATTAAAGGGAGGAAGATGAATATGCATAAAATTCCT
NM_005590.3         AACTATCAAGATGGCAACCTCAACATTTCAATTCCAGTGTTTAGTATTCATGGCAATCAT
XM_005274007.1      AACTATCAAGATGGCAACCTCAACATTTCAATTCCAGTGTTTAGTATTCATGGCAATCAT
                    ** :*:**:*:*** :: ** .. ***:.*.  . ..:*:* *.*** ***...*:**.*

HsCD00336657        GACGATCCCACAGGGGCAGATGCACTTTGTGCCTTGGACATTTTAAGTTGTGCTGGATTT
XM_005274008.1      CTTCACACAGTGCGGCAGTTTTTCATGGAGGATATTGTTCTAGCTAATCATCCAGACATT
NM_005591.3         CTTCACACAGTGCGGCAGTTTTTCATGGAGGATATTGTTCTAGCTAATCATCCAGACATT
NM_005590.3         GACGATCCCACAGGGGCAGATGCACTTTGTGCCTTGGACATTTTAAGTTGTGCTGGATTT
XM_005274007.1      GACGATCCCACAGGGGCAGATGCACTTTGTGCCTTGGACATTTTAAGTTGTGCTGGATTT
                     :  * .*.. . ** .. :*  ..*  . *. :* *: .*:  :*.* .* *:*..:**

HsCD00336657        GTAAATCACTTTGGACGTTCAATGTCTGTGGAGAAGATAGACATTAGTCCGGTTTTGCTT
XM_005274008.1      TTTAACCCAGATAATCCTAAAGTAACCCAAGCCATACAAAGCTTCTGTTTGGAGAAGATT
NM_005591.3         TTTAACCCAGATAATCCTAAAGTAACCCAAGCCATACAAAGCTTCTGTTTGGAGAAGATT
NM_005590.3         GTAAATCACTTTGGACGTTCAATGTCTGTGGAGAAGATAGACATTAGTCCGGTTTTGCTT
XM_005274007.1      GTAAATCACTTTGGACGTTCAATGTCTGTGGAGAAGATAGACATTAGTCCGGTTTTGCTT
                     *:** *.. :*..:* *:.*.*.:*  :.*. *:..:*..*:* :**  **: ::*.**

HsCD00336657        CAAAAAGGAAGCACAAAGATTGCGCTATATGGTTTAG-----------------------
XM_005274008.1      GAAGAAATGCTTGAAAATGCTGAACGGGAACGTCTGGGTAATTCTCACCAGCCAGAGAAG
NM_005591.3         GAAGAAATGCTTGAAAATGCTGAACGGGAACGTCTGGGTAATTCTCACCAGCCAGAGAAG
NM_005590.3         CAAAAAGGAAGCACAAAGATTGCGCTATATGGTTTAG-----------------------
XM_005274007.1      CAAAAAGGAAGCACAAAGATTGCGCTATATGGTTTAG-----------------------
                     **.**. ..  ..*** . **..* . *: ** *.*                       

HsCD00336657        --------------------GATCCATTCCAGATGAAAGGCTCTATCGAATGTTTGTCAA
XM_005274008.1      CCTCTTGTACGACTGCGAGTGGACTATAGTGGAGGTTTTGAACCTTTCAGTGTTCTTCGC
NM_005591.3         CCTCTTGTACGACTGCGAGTGGACTATAGTGGAGGTTTTGAACCTTTCAGTGTTCTTCGC
NM_005590.3         --------------------GATCCATTCCAGATGAAAGGCTCTATCGAATGTTTGTCAA
XM_005274007.1      --------------------GATCCATTCCAGATGAAAGGCTCTATCGAATGTTTGTCAA
                                        *.:* **:  .** *::: *.:* :*  *.****  **..

HsCD00336657        TAAAAAAGTAACAATGTTGAGACCAAAGGAAGATGAGAACTCTTGGTTTAACTTATTTGT
XM_005274008.1      TTTAGCCAGAAATTTGTGGATCGGGTAGCTAATCCAAAAGACATTATCCATTTTTTCAGG
NM_005591.3         TTTAGCCAGAAATTTGTGGATCGGGTAGCTAATCCAAAAGACATTATCCATTTTTTCAGG
NM_005590.3         TAAAAAAGTAACAATGTTGAGACCAAAGGAAGATGAGAACTCTTGGTTTAACTTATTTGT
XM_005274007.1      TAAAAAAGTAACAATGTTGAGACCAAAGGAAGATGAGAACTCTTGGTTTAACTTATTTGT
                    *::*.... **.::*** ** .  .:** :*.:  *.** :*:* .*  *: **:* :* 

HsCD00336657        GATTCATCAGAACAGGAGTAAACATGGAAGTACTAACTTCATTCCAGAACAATTTTTGGA
XM_005274008.1      CATAGAGAACAAAAGGAAAAAACAGG-AGAAGAGATCAACTTTGGGAAACTTATCACAAA
NM_005591.3         CATAGAGAACAAAAGGAAAAAACAGG-AGAAGAGATCAACTTTGGGAAACTTATCACAAA
NM_005590.3         GATTCATCAGAACAGGAGTAAACATGGAAGTACTAACTTCATTCCAGAACAATTTTTGGA
XM_005274007.1      GATTCATCAGAACAGGAGTAAACATGGAAGTACTAACTTCATTCCAGAACAATTTTTGGA
                     **: * .* **.****.:***** * *..:.. *:*::*:**  ..***:::* : ..*

HsCD00336657        TGACTTCATTGATCTTGTTATCTGGGGCCATGAACATGAGTGTAAAATAGCTCCAACCAA
XM_005274008.1      GCCTTCAGAAGGAACAACTTTAAGGGTAGAAGATCTTGTAAAACAGTACTTTCAAACCGC
NM_005591.3         GCCTTCAGAAGGAACAACTTTAAGGGTAGAAGATCTTGTAAAACAGTACTTTCAAACCGC
NM_005590.3         TGACTTCATTGATCTTGTTATCTGGGGCCATGAACATGAGTGTAAAATAGCTCCAACCAA
XM_005274007.1      TGACTTCATTGATCTTGTTATCTGGGGCCATGAACATGAGTGTAAAATAGCTCCAACCAA
                      . * ..::*.:. :. *:*.:*** . *:**:*:**:.:.:.*.::.  **.****..

HsCD00336657        AAATGAACAACAGCTGTTTTATATCTCACAACCTGGAAGCTCAGTGGTTACTTCTCTTTC
XM_005274008.1      AGAGAAGAATGTGCAGCTCTCACTGCTAACAGAAAGAGGGATGGGTGAAGCAGTACAAGA
NM_005591.3         AGAGAAGAATGTGCAGCTCTCACTGCTAACAGAAAGAGGGATGGGTGAAGCAGTACAAGA
NM_005590.3         AAATGAACAACAGCTGTTTTATATCTCACAACCTGGAAGCTCAGTGGTTACTTCTCTTTC
XM_005274007.1      AAATGAACAACAGCTGTTTTATATCTCACAACCTGGAAGCTCAGTGGTTACTTCTCTTTC
                    *.* .*..*: :**:* * *.:.*   *..* .:.**.* : .*  *::.*:  :*:: .

HsCD00336657        CCCAGGAGAAGCTGTAAAGAAACATGTTGGTTTGCTGCGTATTAAAGGGAGGAAGATGAA
XM_005274008.1      ATTTGTGGACAAGGAGGAGAAAGATGCCATTGAGGAATTAGTGAAATACCAGTTGGAAAA
NM_005591.3         ATTTGTGGACAAGGAGGAGAAAGATGCCATTGAGGAATTAGTGAAATACCAGTTGGAAAA
NM_005590.3         CCCAGGAGAAGCTGTAAAGAAACATGTTGGTTTGCTGCGTATTAAAGGGAGGAAGATGAA
XM_005274007.1      CCCAGGAGAAGCTGTAAAGAAACATGTTGGTTTGCTGCGTATTAAAGGGAGGAAGATGAA
                    .  :* .**... *:..***** ***  . * :* :.  :.* *** . ..*::*.:.**

HsCD00336657        TATGCATAAAATTCCTCTTCACACAGTGCGGCAGTTTTTCATGGAGGATATTGTTCTAGC
XM_005274008.1      AACACAGCGATTTCTTAAAGAACGTCATATTGATGCCCTCGAAGACAAAATCGATGAGGA
NM_005591.3         AACACAGCGATTTCTTAAAGAACGTCATATTGATGCCCTCGAAGACAAAATCGATGAGGA
NM_005590.3         TATGCATAAAATTCCTCTTCACACAGTGCGGCAGTTTTTCATGGAGGATATTGTTCTAGC
XM_005274007.1      TATGCATAAAATTCCTCTTCACACAGTGCGGCAGTTTTTCATGGAGGATATTGTTCTAGC
                    :* .** ..*:*** *.:: *.. : : .   *     **.:.** .*:** *:* :.*.

HsCD00336657        TAATCATCCAGACATTTTTAACCCAGATAATCCTAAAGTAACCCAAGCCATACAAAGCTT
XM_005274008.1      GGTACGTCGTTTCAGAGAAACCAGACAAAAAAATACTAATGAAGAAGATGATGAAGTCCG
NM_005591.3         GGTACGTCGTTTCAGAGAAACCAGACAAAAAAATACTAATGAAGAAGATGATGAAGTCCG
NM_005590.3         TAATCATCCAGACATTTTTAACCCAGATAATCCTAAAGTAACCCAAGCCATACAAAGCTT
XM_005274007.1      TAATCATCCAGACATTTTTAACCCAGATAATCCTAAAGTAACCCAAGCCATACAAAGCTT
                     .::*.** : :** : ::*.*. * *:**:..**.:.::... ***. .:: **. *  

HsCD00336657        CTG---------------------------------------------------------
XM_005274008.1      TGAGGCTATGACCAGGGCCAGAGCACTCAGATCTCAGTCAGAGGAGTCTGCTTCTGCCTT
NM_005591.3         TGAGGCTATGACCAGGGCCAGAGCACTCAGATCTCAGTCAGAGGAGTCTGCTTCTGCCTT
NM_005590.3         CTG---------------------------------------------------------
XM_005274007.1      CTG---------------------------------------------------------
                      .                                                         

HsCD00336657        ------------------------------------------------------------
XM_005274008.1      TAGTGCTGATGACCTTATGAGTATAGATTTAGCAGAACAGATGGCTAATGACTCTGATGA
NM_005591.3         TAGTGCTGATGACCTTATGAGTATAGATTTAGCAGAACAGATGGCTAATGACTCTGATGA
NM_005590.3         ------------------------------------------------------------
XM_005274007.1      ------------------------------------------------------------
                                                                                

HsCD00336657        ----------------------TTTGGAGAAGATTGAAGAAATGCTTGAAAATGCTGAAC
XM_005274008.1      TAGCATCTCAGCAGCAACCAACAAAGGAAGAGGCCGAGGAAGAGGTCGAAGAGGTGGAAG
NM_005591.3         TAGCATCTCAGCAGCAACCAACAAAGGAAGAGGCCGAGGAAGAGGTCGAAGAGGTGGAAG
NM_005590.3         ----------------------TTTGGAGAAGATTGAAGAAATGCTTGAAAATGCTGAAC
XM_005274007.1      ----------------------TTTGGAGAAGATTGAAGAAATGCTTGAAAATGCTGAAC
                                          :::***..**.  **.***.:* * ***.* *  *** 

HsCD00336657        GGGAAGGGCATCATCACCATCACCACTGA-------------------------------
XM_005274008.1      AGGGCAGAATTCAGCATCGAGAGGAGGGTCTCAAAGAGGAAGAGCAGACACTGGTCTGGA
NM_005591.3         AGGGCAGAATTCAGCATCGAGAGGAGGGTCTCAAAGAGGAAGAGCAGACACTGGTCTGGA
NM_005590.3         GGGAACGTCTGGGTAATTCTCACCAGCCAGAGAAGCCTCTTGTACGACTGCGAGTGGACT
XM_005274007.1      GGGAACGTCTGGGTAATTCTCACCAGCCAGAGAAGCCTCTTGTACGACTGCGAGTGGACT
                    .**.. * .:  . .*   : *  *   :                               

HsCD00336657        ------------------------------------------------------------
XM_005274008.1      GACTTCTACCCGTAGCAGGAACTCAAAGACTGCTGTGTCAGCATCTAGAAATATGTCTAT
NM_005591.3         GACTTCTACCCGTAGCAGGAACTCAAAGACTGCTGTGTCAGCATCTAGAAATATGTCTAT
NM_005590.3         ATAGTGGAGGTTTTGAACCTTTCAGTGTTCTTCGCTTTAGCCAGAAATTTGTGGATCGGG
XM_005274007.1      ATAGTGGAGGTTTTGAACCTTTCAGTGTTCTTCGCTTTAGCCAGAAATTTGTGGATCGGG
                                                                                

HsCD00336657        ------------------------------------------------------------
XM_005274008.1      TATAGATGCCTTTAAATCTACAAGACAGCAGCCTTCCCGAAATGTCACTACTAAGAATTA
NM_005591.3         TATAGATGCCTTTAAATCTACAAGACAGCAGCCTTCCCGAAATGTCACTACTAAGAATTA
NM_005590.3         TAGCTAATCCAAAAGACATTATCCATTTTTTCAGGCATAGAGAACAAAAGGAAAAAACAG
XM_005274007.1      TAGCTAATCCAAAAGACATTATCCATTTTTTCAGGCATAGAGAACAAAAGGAAAAAACAG
                                                                                

HsCD00336657        ------------------------------------------------------------
XM_005274008.1      TTCAGAGGTGATTGAGGTAGATGAATCAGATGTGGAAGAAGACATTTTTCCTACCACTTC
NM_005591.3         TTCAGAGGTGATTGAGGTAGATGAATCAGATGTGGAAGAAGACATTTTTCCTACCACTTC
NM_005590.3         GAGAAGAGATCAACTTTGGGAAACTTATCACAAAGCCTTCAGAAGGAACAACTTTAAGGG
XM_005274007.1      GAGAAGAGATCAACTTTGGGAAACTTATCACAAAGCCTTCAGAAGGAACAACTTTAAGGG
                                                                                

HsCD00336657        ------------------------------------------------------------
XM_005274008.1      AAAGACAGATCAAAGGTGGTCCAGCACATCATCCAGCAAAATCATGTCCCAGAGTCAAGT
NM_005591.3         AAAGACAGATCAAAGGTGGTCCAGCACATCATCCAGCAAAATCATGTCCCAGAGTCAAGT
NM_005590.3         TAGAAGATCTTGTAAAACAGTACTTTCAAACCGCAGAGAAGAATGTGCAGCTCTCACTGC
XM_005274007.1      TAGAAGATCTTGTAAAACAGTACTTTCAAACCGCAGAGAAGAATGTGCAGCTCTCACTGC
                                                                                

HsCD00336657        ------------------------------------------------------------
XM_005274008.1      ATCGAAAGGGGTTGATTTTGAATCAAGTGAGGATGATGATGATGATCCTTTTATGAACAC
NM_005591.3         ATCGAAAGGGGTTGATTTTGAATCAAGTGAGGATGATGATGATGATCCTTTTATGAACAC
NM_005590.3         TAACAGAAAGAGGGATGGGTGAAGCAGTACAAGAATTTGTGGACAAGGAGGAGAAAGATG
XM_005274007.1      TAACAGAAAGAGGGATGGGTGAAGCAGTACAAGAATTTGTGGACAAGGAGGAGAAAGATG
                                                                                

HsCD00336657        ------------------------------------------------------------
XM_005274008.1      TAGTTCTTTAAGAAGAAATAGAAGATAA--------------------------------
NM_005591.3         TAGTTCTTTAAGAAGAAATAGAAGATAA--------------------------------
NM_005590.3         CCATTGAGGAATTAGTGAAATACCAGTTGGAAAAAACACAGCGATTTCTTAAAGAACGTC
XM_005274007.1      CCATTGAGGAATTAGTGAAATACCAGTTGGAAAAAACACAGCGATTTCTTAAAGAACGTC
                                                                                

HsCD00336657        ------------------------------------------------------------
XM_005274008.1      ------------------------------------------------------------
NM_005591.3         ------------------------------------------------------------
NM_005590.3         ATATTGATGCCCTCGAAGACAAAATCGATGAGGAGGTACGTCGTTTCAGAGAAACCAGAC
XM_005274007.1      ATATTGATGCCCTCGAAGACAAAATCGATGAGGAGGTACGTCGTTTCAGAGAAACCAGAC
                                                                                

HsCD00336657        ------------------------------------------------------------
XM_005274008.1      ------------------------------------------------------------
NM_005591.3         ------------------------------------------------------------
NM_005590.3         AAAAAAATACTAATGAAGAAGATGATGAAGTCCGTGAGGCTATGACCAGGGCCAGAGCAC
XM_005274007.1      AAAAAAATACTAATGAAGAAGATGATGAAGTCCGTGAGGCTATGACCAGGGCCAGAGCAC
                                                                                

HsCD00336657        ------------------------------------------------------------
XM_005274008.1      ------------------------------------------------------------
NM_005591.3         ------------------------------------------------------------
NM_005590.3         TCAGATCTCAGTCAGAGGAGTCTGCTTCTGCCTTTAGTGCTGATGACCTTATGAGTATAG
XM_005274007.1      TCAGATCTCAGTCAGAGGAGTCTGCTTCTGCCTTTAGTGCTGATGACCTTATGAGTATAG
                                                                                

HsCD00336657        ------------------------------------------------------------
XM_005274008.1      ------------------------------------------------------------
NM_005591.3         ------------------------------------------------------------
NM_005590.3         ATTTAGCAGAACAGATGGCTAATGACTCTGATGATAGCATCTCAGCAGCAACCAACAAAG
XM_005274007.1      ATTTAGCAGAACAGATGGCTAATGACTCTGATGATAGCATCTCAGCAGCAACCAACAAAG
                                                                                

HsCD00336657        ------------------------------------------------------------
XM_005274008.1      ------------------------------------------------------------
NM_005591.3         ------------------------------------------------------------
NM_005590.3         GAAGAGGCCGAGGAAGAGGTCGAAGAGGTGGAAGAGGGCAGAATTCAGCATCGAGAGGAG
XM_005274007.1      GAAGAGGCCGAGGAAGAGGTCGAAGAGGTGGAAGAGGGCAGAATTCAGCATCGAGAGGAG
                                                                                

HsCD00336657        ------------------------------------------------------------
XM_005274008.1      ------------------------------------------------------------
NM_005591.3         ------------------------------------------------------------
NM_005590.3         GGTCTCAAAGAGGAAGAGCCTTTAAATCTACAAGACAGCAGCCTTCCCGAAATGTCACTA
XM_005274007.1      GGTCTCAAAGAGGAAGAGCAGACACTGGTCTGGAGACTTCTACCCGTAGCAGGAACTCAA
                                                                                

HsCD00336657        ------------------------------------------------------------
XM_005274008.1      ------------------------------------------------------------
NM_005591.3         ------------------------------------------------------------
NM_005590.3         CTAAGAATTATTCAGAGGTGATTGAGGTAGATGAATCAGATGTGGAAGAAGACATTTTTC
XM_005274007.1      AGACTGCTGTGTCAGCATCTAGAAATATGTCTATTATAGATGCCTTTAAATCTACAAGAC
                                                                                

HsCD00336657        ------------------------------------------------------------
XM_005274008.1      ------------------------------------------------------------
NM_005591.3         ------------------------------------------------------------
NM_005590.3         CTACCACTTCAAAGACAGATCAAAGGTGGTCCAGCACATCATCCAGCAAAATCATGTCCC
XM_005274007.1      AGCAGCCTTCCCGAAATGTCACTACTAAGAATTATTCAGAGGTGATTGAGGTAGATGAAT
                                                                                

HsCD00336657        ------------------------------------------------------------
XM_005274008.1      ------------------------------------------------------------
NM_005591.3         ------------------------------------------------------------
NM_005590.3         AGAGTCAAGTATCGAAAGGGGTTGATTTTGAATCAAGTGAGGATGATGATGATGATCCTT
XM_005274007.1      CAGATGTGGAAGAAGACATTTTTCCTACCACTTCAAAGACAGATCAAAGGTGGTCCAGCA
                                                                                

HsCD00336657        ------------------------------------------------------------
XM_005274008.1      ------------------------------------------------------------
NM_005591.3         ------------------------------------------------------------
NM_005590.3         TTATGAACACTAGTTCTTTAAGAAGAAATAGAAGATAA----------------------
XM_005274007.1      CATCATCCAGCAAAATCATGTCCCAGAGTCAAGTATCGAAAGGGGTTGATTTTGAATCAA
                                                                                

HsCD00336657        ------------------------------------------------------------
XM_005274008.1      ------------------------------------------------------------
NM_005591.3         ------------------------------------------------------------
NM_005590.3         ------------------------------------------------------------
XM_005274007.1      GTGAGGATGATGATGATGATCCTTTTATGAACACTAGTTCTTTAAGAAGAAATAGAAGAT
                                                                                

HsCD00336657        --
XM_005274008.1      --
NM_005591.3         --
NM_005590.3         --
XM_005274007.1      AA

Pathways

NCI :

ATM pathway

NCI :

BARD1 signaling events

NCI :

Fanconi anemia pathway

NCI :

Regulation of Telomerase

NCI :

Validated transcriptional targets of deltaNp63 isoforms

Reactome :

Assembly of the RAD50-MRE11-NBS1 complex at DNA double-strand breaks

Reactome :

Cell Cycle

Reactome :

Cellular responses to stress

Reactome :

Cellular Senescence

Reactome :

Cytosolic sensors of pathogen-associated DNA

Reactome :

DNA Damage/Telomere Stress Induced Senescence

Reactome :

DNA Repair

Reactome :

Double-Strand Break Repair

Reactome :

Homologous Recombination Repair

Reactome :

Homologous recombination repair of replication-independent double-strand breaks

Reactome :

Immune System

Reactome :

Innate Immune System

Reactome :

IRF3-mediated induction of type I IFN

Reactome :

Meiosis

Reactome :

Meiotic recombination

Reactome :

MRN complex relocalizes to nuclear foci

Reactome :

Recruitment of repair and signaling proteins to double-strand breaks

Reactome :

STING mediated induction of host immune responses

WikiPathway :

DNA damage response

WikiPathway :

Homologous recombination

WikiPathway :

Integrated Breast Cancer Pathway

WikiPathway :

Integrated Cancer pathway

WikiPathway :

miRNA regulation of DNA Damage Response

WikiPathway :

Non-homologous end joining

Protein

Structural Annotation Wiki :

TOPSAN

UniProt :

Q05D78

UniProt :

P49959

HPRD :

02889

Clone

Cloning Information :

495274

HIP Master Clone ID :

408954

Original Clone ID :

8658a8BCt5p1

Experimental

TargetTrack - Experimental data :

NYSGXRC-8658a

Organism

Species Specific ID:

4361

Genome

Chromosome :

Map Location :

11q21

Ensembl :

ENSG00000020922

Reagents

Labome :

Mre11-antibody

MRE11A (H. sapiens )In pSGX3 (His-tagged bacterial expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Reference Sequence Alignment

Pathways

Protein

Clone

Experimental

Organism

Genome

Reagents

MRE11A (H. sapiens )
In pSGX3 (His-tagged bacterial expression vector)