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Detailed Vector Information: pET15TEV_NESG

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pET15TEV_NESG              
Source: Northeast Structural Genomics Consortium
Description: Bacterial (E. coli) expresson vector, N-terminal TEV-cleavable 6x-adds His tag; ampicillin resistance; restriction enzyme cloning.
Comments: None
Publications: PMID: 26405037
Title: Structural Studies of IRF4 Reveal a Flexible Autoinhibitory Region and a Compact Linker Domain
Authors: Northeast Structural Genomics Consortium
Gaetano T. Montleione
Thomas B. Acton
EvNO00338203

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Academic and non-profit labs
Special MTA: PSI/NESG

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
       DyNA Vector Map

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Vector Name: pET15TEV_NESG

pET15TEV_NESG in advanced viewed

Synonyms: pET15TEV, pET15TEV_NESG
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial (E. coli) expresson vector, N-terminal TEV-cleavable 6x-adds His tag; ampicillin resistance; restriction enzyme cloning.
Comments: None
Size (bp): 5700
Parent Vector: None
Properties: bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: Northeast Structural Genomics Consortium
Gaetano T. Montleione
Thomas B. Acton
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR322 bacterial origin 4396 4578
primer T7 forward T7 forward sequencing primer 496 511
primer T7 reverse T7 reverse sequencing primer 257 275
promoter T7 T7 promoter 493 512
protease cleavage site TEV N-terminal TEV protease cleavage site 381 401
selectable marker AmpR Ampicillin resistance gene 4676 5335
tag His N-terminal 6xHis tag (TEV protease cleavable) 402 419
trxn termination sequence T7 term T7 transcriptional termination 217 263

 

Species Specific ID: None
Target GenBank: None