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AF0783 (Archaeoglobus fulgidus) in pET21_NESG (His-tagged bacterial expression vector)

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Explanation of Terms


Gene: Gene Symbol:  AF0783
Gene Name:  hypothetical protein
Sequence             
Original Clone ID: GR44-21.1
Keyword: None
Species: Archaeoglobus fulgidus
Type: cDNA
Vector Name: pET21_NESG               Format:  FUSION
Source: Northeast Structural Genomics Consortium
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: PSI
Northeast Structural Genomics Consortium
AsCD00338705

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AVAILABLE
No restriction
Special MTA: PSI/NESG

 

Insert sequence: 579nts         Open reading frame : 1 to 579

ATGATTCCCTGCCCGAGCTGCGGAAGGGAGCTGAGGGCGGTAGTATCAACCTACGACGTT
CCGTTTTTCGGCAAGGTTCTTCTCACGTCGATTAGCTGTGAGTGCGGTTTCAGACACGCG
GATTCCGTTGTTCTCGGAGAAAAGGACCCGGTGAGATACATCATAAAAATAAACAGGGAG
AACCTCTTCACCAAGGTTATAAGGTCCACTTCAGGAACCATACGCATTCCCGAAATCGGG
GTGGCAATTGAGCCCGGCCCGGCAAGCCAGGCCTTCATCACAAACCTCGAAGGCGTGCTG
GACAGGGTGGAGGGTGTTGTTAGAACAGCCATGCGCTGGAACTCCGATGACGAGGAGAAG
GTGAGGAGGTGCGAGTGGATTCTCGAAAGAATCAGAAACACGATTGACGGAGACGAGGAG
CTCACTCTCATTCTTGAGGACCCCTTCGGTAACAGCCTAATCGTTTCGGACGAGGCTTTC
AAGGAGATAATGTCCAAGGAGGAGGCTCAGAGGCTCAAAACCGGGATGACCATAATCGAT
ATAACCGGAATGAGCGAGGAGGAGCTGGAGGATTTAGTC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
AAGGAGATATACAT

3' Linker Sequence:
CTCGAGCACCACCACCACCACCACTGA

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: ProteinExpressed: Not_Tested
ProteinPurified: Not_Tested
SolubleProtein: Not_Tested
Find experimental protocols in GR44
Recommended expression in: E. coli BL21
       DyNA Vector Map

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Vector Name: pET21_NESG

pET21_NESG in advanced viewed

Synonyms: pET21, pET21-modified, pET21Cc, pET21_NESG
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial (E. coli) expression vector with T7 lac promoter, adds C-terminal 6xHis tag; ampicillin resistance; restriction enzyme cloning.
Comments: Modified Novagen pET vector-common MCS
Size (bp): 5415
Parent Vector: None
Empty Vector: EvNO00336944
Properties: bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker
Author Name: Northeast Structural Genomics Consortium
Gaetano T. Montleione
Thomas B. Acton
Publications: PMID: 15808222
Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS MCS (includes SphI, XbaI, BamHI, EcoRI, XhoI) 157 253
bacterial origin ori pBR322-type bacterial origin of replication 3180 3862
gene fragment ROP ROP 2580 2756
primer T7 forward T7 forward sequencing primer 283 298
primer T7 reverse T7 reverse sequencing primer 66 84
promoter T7 T7 promoter 280 299
repressor binding site lacO lacO regulatory sequence 258 280
repressor protein gene LacI LacI 752 1768
selectable marker AmpR ampicillin resistance gene 3960 4619
ssDNA origin f1 ori f1 origin 5081 5387
tag His C-terminal 6xHis tag 140 157
trxn termination sequence T7 term T7 termination sequence 68 72

 

  • Gene
  • Protein
  • Clone
  • Experimental
  • Organism
  • Reagents

Gene

 

Gene Symbol : AF0783
GENEID : 1484000
Locus Tag : AF0783
GI : 11498389
Target GenBank: None

 

Protein

SMART domain : Zpr1 : Duplicated domain in Zpr1. Also present in archaeal proteins.
Structural Annotation Wiki : TOPSAN
Swissprot ID : O29475_ARCFU
UniProt : O29475

Clone

Cloning Information : 544372
HIP Master Clone ID : 454692
Original Clone ID : GR44-21.1

Experimental

TargetTrack - Experimental data : GR44

Organism


TAX_ID : 224325
Species Specific ID: 1484000

Reagents

Labome : AF0783-cDNA