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Detailed Plasmid Information

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Explanation of Terms


Gene: Gene Symbol:  CA8
Gene Name:  carbonic anhydrase VIII
Sequence             
Original Clone ID: HR3028B-14.2
Keyword: full length protein truncation
Species: Homo sapiens
Type: cDNA
Vector Name: pET15_NESG               Format:  CLOSED
Source: Northeast Structural Genomics Consortium
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors: PSI
Northeast Structural Genomics Consortium
HsCD00339468

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AVAILABLE
No restriction
Special MTA: PSI/NESG

 

Insert sequence: 807nts         Open reading frame : 1 to 807
Reference Sequence Alignment

ATGGAGGAGGGTGTGGAGTGGGGCTACGAGGAAGGTGTTGAGTGGGGTCTGGTGTTTCCT
GATGCTAATGGGGAATACCAGTCTCCTATTAACCTAAACTCAAGAGAGGCTAGGTATGAC
CCCTCGCTGTTGGATGTCCGCCTCTCCCCAAATTATGTGGTGTGCCGAGACTGTGAAGTC
ACCAATGATGGACATACCATTCAGGTTATCCTGAAGTCAAAATCAGTTCTTTCGGGAGGA
CCATTGCCTCAAGGGCATGAGTTTGAACTGTACGAAGTGAGATTTCACTGGGGAAGAGAA
AACCAGCGTGGTTCTGAGCACACGGTTAATTTCAAAGCTTTTCCCATGGAGCTCCATCTG
ATCCACTGGAACTCCACTCTGTTTGGCAGCATTGATGAGGCTGTGGGGAAGCCGCACGGA
ATCGCCATCATTGCTCTGTTTGTTCAGATAGGAAAGGAACATGTTGGCTTGAAGGCTGTG
ACTGAAATCCTCCAAGATATTCAGTATAAGGGGAAGTCCAAAACAATACCTTGCTTTAAT
CCTAACACTTTATTACCAGACCCTCTGCTGCGGGATTACTGGGTGTATGAAGGCTCTCTC
ACCATCCCACCTTGCAGTGAAGGTGTCACCTGGATATTATTCCGATACCCTTTAACTATA
TCCCAGCTACAGATAGAAGAATTTCGAAGGCTGAGGACACATGTTAAGGGGGCAGAACTT
GTGGAAGGCTGTGATGGGATTTTGGGAGACAACTTTCGGCCCACTCAGCCTCTTAGTGAC
AGAGTCATTAGAGCTGCATTTCAGTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
c130t,L44L
                                

5' Linker Sequence:
ATGGGCCATCACCATCACCATCACAGCCAT

3' Linker Sequence:
CTCGAGATCCGGCTGC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: ProteinExpressed: Protein_Confirmed
ProteinPurified: Not_Tested
SolubleProtein: Protein_Soluble
Find experimental protocols in HR3028B
Recommended expression in: E. coli BL21
       DyNA Vector Map

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Vector Name: pET15_NESG

pET15_NESG in advanced viewed

Synonyms: pET15, pET15-modified, pET15Cc, pET15_NESG
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial (E. coli) expression vector with T7 lac promoter, adds N-terminal 6x His tag; amp resistance; restriction enzyme cloning.
Comments: Modified Novagen pET vector-common MCS
Size (bp): 5723
Parent Vector: None
Empty Vector: EvNO00336943
Properties: bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker
Author Name: Northeast Structural Genomics Consortium
Gaetano T. Montleione
Jessica Bearden
Publications: PMID: 15808222
Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS MCS (includes SphI, XbaI, EcoRI, BamHI, XhoI) 327 452
bacterial origin ori ColE1 origin of replication 3881 4563
gene fragment ROP repressor of primer ROP sequence 3281 3457
primer T7 forward T7 forward sequencing primer 481 496
primer T7 reverse T7 reverse sequencing primer 257 275
promoter T7 T7 promoter 478 497
repressor binding site lacO lacO regulatory sequence 456 478
selectable marker AmpR ampicillin resistance gene 4661 5230
tag His N-terminal 6x His tag 387 404
trxn termination sequence T7 term T7 termination sequence 257 275

 

  • Gene
  • Protein
  • Clone
  • Experimental
  • Organism
  • Genome

Gene

 

Gene Symbol : CA8
Symbol Nomenclature : CA8
SYNONYM : CA-VIII; CALS; CAMRQ3; CARP
Designation : CA-related protein|carbonate dehydratase|carbonic anhydrase-like sequence|carbonic anhydrase-related protein
Full Nomenclature : carbonic anhydrase VIII
GENEID : 767
HGNC : 1382
MIM : 114815
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00339468      ------------------------------------------------------------
NM_004056.4       ATGGCGGACCTGAGCTTCATCGAAGATACCGTCGCCTTCCCCGAGAAGGAAGAGGATGAG
                                                                              

HsCD00339468      ------ATGGAGGAGGGTGTGGAGTGGGGCTACGAGGAAGGTGTTGAGTGGGGTCTGGTG
NM_004056.4       GAGGAAGAAGAGGAGGGTGTGGAGTGGGGCTACGAGGAAGGTGTTGAGTGGGGTCTGGTG
                        .:.***************************************************

HsCD00339468      TTTCCTGATGCTAATGGGGAATACCAGTCTCCTATTAACCTAAACTCAAGAGAGGCTAGG
NM_004056.4       TTTCCTGATGCTAATGGGGAATACCAGTCTCCTATTAACCTAAACTCAAGAGAGGCTAGG
                  ************************************************************

HsCD00339468      TATGACCCCTCGCTGTTGGATGTCCGCCTCTCCCCAAATTATGTGGTGTGCCGAGACTGT
NM_004056.4       TATGACCCCTCGCTGTTGGATGTCCGCCTCTCCCCAAATTATGTGGTGTGCCGAGACTGT
                  ************************************************************

HsCD00339468      GAAGTCACCAATGATGGACATACCATTCAGGTTATCCTGAAGTCAAAATCAGTTCTTTCG
NM_004056.4       GAAGTCACCAATGATGGACATACCATTCAGGTTATCCTGAAGTCAAAATCAGTTCTTTCG
                  ************************************************************

HsCD00339468      GGAGGACCATTGCCTCAAGGGCATGAGTTTGAACTGTACGAAGTGAGATTTCACTGGGGA
NM_004056.4       GGAGGACCATTGCCTCAAGGGCATGAATTTGAACTGTACGAAGTGAGATTTCACTGGGGA
                  **************************.*********************************

HsCD00339468      AGAGAAAACCAGCGTGGTTCTGAGCACACGGTTAATTTCAAAGCTTTTCCCATGGAGCTC
NM_004056.4       AGAGAAAACCAGCGTGGTTCTGAGCACACGGTTAATTTCAAAGCTTTTCCCATGGAGCTC
                  ************************************************************

HsCD00339468      CATCTGATCCACTGGAACTCCACTCTGTTTGGCAGCATTGATGAGGCTGTGGGGAAGCCG
NM_004056.4       CATCTGATCCACTGGAACTCCACTCTGTTTGGCAGCATTGATGAGGCTGTGGGGAAGCCG
                  ************************************************************

HsCD00339468      CACGGAATCGCCATCATTGCTCTGTTTGTTCAGATAGGAAAGGAACATGTTGGCTTGAAG
NM_004056.4       CACGGAATCGCCATCATTGCTCTGTTTGTTCAGATAGGAAAGGAACATGTTGGCTTGAAG
                  ************************************************************

HsCD00339468      GCTGTGACTGAAATCCTCCAAGATATTCAGTATAAGGGGAAGTCCAAAACAATACCTTGC
NM_004056.4       GCTGTGACTGAAATCCTCCAAGATATTCAGTATAAGGGGAAGTCCAAAACAATACCTTGC
                  ************************************************************

HsCD00339468      TTTAATCCTAACACTTTATTACCAGACCCTCTGCTGCGGGATTACTGGGTGTATGAAGGC
NM_004056.4       TTTAATCCTAACACTTTATTACCAGACCCTCTGCTGCGGGATTACTGGGTGTATGAAGGC
                  ************************************************************

HsCD00339468      TCTCTCACCATCCCACCTTGCAGTGAAGGTGTCACCTGGATATTATTCCGATACCCTTTA
NM_004056.4       TCTCTCACCATCCCACCTTGCAGTGAAGGTGTCACCTGGATATTATTCCGATACCCTTTA
                  ************************************************************

HsCD00339468      ACTATATCCCAGCTACAGATAGAAGAATTTCGAAGGCTGAGGACACATGTTAAGGGGGCA
NM_004056.4       ACTATATCCCAGCTACAGATAGAAGAATTTCGAAGGCTGAGGACACATGTTAAGGGGGCA
                  ************************************************************

HsCD00339468      GAACTTGTGGAAGGCTGTGATGGGATTTTGGGAGACAACTTTCGGCCCACTCAGCCTCTT
NM_004056.4       GAACTTGTGGAAGGCTGTGATGGGATTTTGGGAGACAACTTTCGGCCCACTCAGCCTCTT
                  ************************************************************

HsCD00339468      AGTGACAGAGTCATTAGAGCTGCATTTCAGTAG
NM_004056.4       AGTGACAGAGTCATTAGAGCTGCATTTCAGTAG
                  *********************************

Protein

SMART domain : Carb_anhydrase : Eukaryotic-type carbonic anhydrase
Structural Annotation Wiki : TOPSAN
Swissprot ID : CAH8_HUMAN
HPRD : 00266

Clone

Cloning Information : 546414
HIP Master Clone ID : 456734
Original Clone ID : HR3028B-14.2

Experimental

TargetTrack - Experimental data : HR3028B

Organism


TAX_ID : 9606
Species Specific ID: None

Genome

Chromosome : 8
Map Location : 8q12.1