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Detailed Vector Information: pMCSG26

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pMCSG26              
Source: Midwest Center for Structural Genomics
Description: Bacterial expression vector with C-terminal 6xHis tag and T7 promoter; ampicillin resistance in bacterial; ligation independent cloning (LIC)
Comments: None
Publications: PMID: 20213425
Title: Cleavable C-terminal His-tag vectors for structure determination.
PMID: 18955706
Title: Structural and functional analysis of AsbF: origin of the stealth 3,4-dihydroxybenzoic acid subunit for petrobactin biosynthesis
Authors: PSI
Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
EvNO00340963

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Special MTA: PSI

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
       DyNA Vector Map

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Vector Name: pMCSG26

pMCSG26 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial expression vector with C-terminal 6xHis tag and T7 promoter; ampicillin resistance in bacterial; ligation independent cloning (LIC)
Comments: For more information about MCSG vectors visit their website at: http://bioinformatics.anl.gov/mcsg/technologies/vectors.html
Size (bp): 5226
Parent Vector: None
Properties: bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: PSI
Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
Publications: PMID: 20213425
Title: Cleavable C-terminal His-tag vectors for structure determination.

PMID: 18955706
Title: Structural and functional analysis of AsbF: origin of the stealth 3,4-dihydroxybenzoic acid subunit for petrobactin biosynthesis

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR322 origin of replication 3220 3220
primer T7 forward T7 forward sequencing primer 299 315
primer T7 reverse T7 terminator reverse sequencing primer 66 84
promoter T7 T7 promoter 300 316
recombination site LIC LIC site 217 238
repressor binding site lac operator LacO fragment 275 297
repressor protein gene LacI Lac I cds 830 1789
selectable marker AmpR Ampicillin resistance gene 3978 4838
tag His C-terminal 6xHis tag 208 225
trxn termination sequence T7 term T7 terminator 26 72

 

Other Link: MCSG Cloning Information
Other Link: MCSG Vector Information
Species Specific ID: None
Target GenBank: None