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Detailed Vector Information: pMCSG28

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pMCSG28              
Source: Midwest Center for Structural Genomics
Description: Bacterial expression vector with T7 promoter and TEV-cleavage C-terminal 6xHis; ampicillin resistance in bacterial; ligation independent cloning (LIC)
Comments: None
Publications: PMID: 20213425
Title: Cleavable C-terminal His-tag vectors for structure determination.
PMID: 24510647
Title: High-Throughput Cloning and Expression of Integral Membrane Proteins in Escherichia coli.
PMID: 26757830
Title: An immunochemical approach to detect oxidized protein tyrosine phosphatases using a selective C-nucleophile tag
Authors: PSI
Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
EvNO00340964

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Special MTA: PSI

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
       DyNA Vector Map

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Vector Name: pMCSG28

pMCSG28 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial expression vector with T7 promoter and TEV-cleavage C-terminal 6xHis; ampicillin resistance in bacterial; ligation independent cloning (LIC)
Comments: For more information about MCSG vectors visit their website at: http://bioinformatics.anl.gov/mcsg/technologies/vectors.html
Size (bp): 5253
Parent Vector: None
Properties: bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: PSI
Midwest Center for Structural Genomics
Argonne National Laboratory
Publications: PMID: 20213425
Title: Cleavable C-terminal His-tag vectors for structure determination.

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR332 origin of replication 3247 3247
primer T7 reverse T7 terminator reverse sequencing primer 66 84
primer T7 forward T7 forward sequencing primer 324 343
promoter T7 T7 promoter (on complementary strand) 327 343
protease cleavage site TEV TEV protease cleavage site (on complementary strand) 232 252
recombination site LIC LIC Site (on complementary strand) 245 265
repressor binding site lac operator LacO fragment 302 324
repressor protein gene LacI LacI regulatory gene 857 1816
selectable marker AmpR Ampicillin resistance 4005 4865
tag His 6xHis tag (on complementary strand) 208 225
trxn termination sequence T7 term T7 terminator (on complementary strand) 26 72

 

Other Link: MCSG Cloning Information
Other Link: MCSG Vector Information
Other Link: SBKB Technology Portal
Species Specific ID: None
Target GenBank: None