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SFRS4 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  SRSF4
Gene Name:  serine/arginine-rich splicing factor 4
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH026101.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00003417

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1485nts         Open reading frame : 1 to 1485

ATGCCGCGGGTGTACATCGGCCGCCTGAGCTACCAGGCCCGGGAGCGCGATGTGGAGCGC
TTCTTTAAGGGCTACGGGAAGATCCTGGAGGTGGATCTGAAGAACGGATATGGTTTTGTG
GAGTTTGATGATCTGCGTGATGCAGATGATGCTGTTTATGAACTGAATGGCAAAGACCTT
TGTGGTGAGCGAGTAATTGTTGAGCATGCCCGCGGCCCACGGCGAGATGGCAGTTACGGT
TCTGGACGCAGTGGATATGGTTATAGAAGAAGTGGCCGAGATAAATATGGCCCTCCTACT
CGCACAGAGTACAGACTTATTGTGGAGAATTTGTCAAGTCGGTGCAGCTGGCAAGACCTA
AAGGATTATATGCGTCAGGCAGGAGAAGTGACTTATGCAGATGCTCACAAGGGACGCAAA
AATGAAGGGGTGATTGAATTTGTATCTTATTCTGATATGAAAAGAGCTTTGGAAAAGTTG
GATGGAACTGAAGTCAATGGGAGAAAAATCAGATTAGTTGAAGACAAGCCAGGTTCCAGA
CGACGCCGGTCCTACTCCAGAAGCCGGAGTCATTCAAGGTCTCGCTCTCGAAGCAGACAT
TCCCGTAAGAGCAGAAGCCGAAGTGGCAGCAGCAAAAGCAGTCATTCTAAGAGTAGATCT
CGGTCCAGGTCGGGCTCCCGCTCCCGGAGCAAGAGCCGGAGCCGGAGCCAGAGTCGGAGC
CGGAGCAAGAAAGAGAAAAGCAGGAGCCCCAGCAAGGAAAAGAGCCGCAGCCGCAGCCAT
AGCGCTGGCAAGAGCCGCAGCAAGAGCAAAGACCAAGCTGAAGAGAAGATCCAAAACAAT
GACAATGTCGGGAAACCCAAGAGCCGGAGTCCTAGCAGGCATAAAAGTAAGAGCAAAAGT
CGGAGCAGGAGTCAGGAGAGGAGAGTGGAGGAGGAGAAGCGAGGGAGTGTGAGCAGGGGC
AGGAGCCAGGAGAAGAGCCTCCGCCAGAGTCGGAGCCGGAGCAGGAGCAAAGGGGGCAGC
AGGAGCCGGAGCAGGAGCCGCAGCAAGAGCAAGGACAAGAGGAAGGGCAGGAAGAGAAGC
AGAGAGGAGAGCCGCAGTCGCAGTCGCAGCCGCAGCAAGAGTGAGAGGAGCAGAAAGCGA
GGCAGCAAGCGAGACAGCAAGGCGGGCAGCAGCAAGAAGAAGAAGAAGGAAGACACTGAC
CGCTCCCAGTCCAGATCTCCATCCCGCTCCGTGTCAAAGGAGCGGGAACATGCCAAGTCT
GAATCCAGCCAGAGGGAAGGTCGAGGAGAGAGTGAGAATGCTGGCACCAATCAGGAGACC
CGGTCCAGGTCGAGATCCAATTCCAAATCGAAACCAAACCTTCCATCAGAATCACGCTCC
AGATCAAAGTCAGCTTCAAAAACCCGATCTCGGTCCAAGTCTAGATCCAGGTCTGCTTCC
AGATCGCCCTCCCGATCTAGATCTAGGTCCCACTCAAGGTCCTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1591
Start on reference sequence 107
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : SRSF4
Symbol Nomenclature : SRSF4
SYNONYM : SFRS4; SRP75
Designation : SR splicing factor 4|SRP001LB|pre-mRNA-splicing factor SRP75|splicing factor, arginine/serine-rich 4
Full Nomenclature : serine/arginine-rich splicing factor 4
GENEID : 6429
GI : 60655746
GenBank Accession : AY889646
HGNC : 10786
MIM : 601940
Vega : OTTHUMG00000003663
Target GenBank: BC002781

 

Pathways

Reactome : Cleavage of Growing Transcript in the Termination Region
Reactome : Gene Expression
Reactome : Post-Elongation Processing of Intron-Containing pre-mRNA
Reactome : Post-Elongation Processing of the Transcript
Reactome : Processing of Capped Intron-Containing Pre-mRNA
Reactome : RNA Polymerase II Transcription
Reactome : RNA Polymerase II Transcription Termination
Reactome : Transcription
Reactome : Transport of Mature Transcript to Cytoplasm
Reactome : Transport of Mature mRNA derived from an Intron-Containing Transcript
Reactome : mRNA 3'-end processing
Reactome : mRNA Splicing
Reactome : mRNA Splicing - Major Pathway
WikiPathway : mRNA processing

Protein

SMART domain : RRM : RNA recognition motif
UniProt : Q08170
HPRD : 09052

Clone

Cloning Information : 26101
HIP Master Clone ID : 56477
Original Clone ID : FLH026101.01X

Organism


TAX_ID : 9606
Species Specific ID: 6429

Genome

Chromosome : 1
Map Location : 1p35.3
Ensembl : ENSG00000116350

Reagents

Labome : SRSF4-antibody