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RFC2 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  RFC2
Gene Name:  replication factor C (activator 1) 2, 40kDa
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH012715.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00003489

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 963nts         Open reading frame : 1 to 963
Reference Sequence Alignment

ATGGAGGTGGAGGCCGTCTGTGGTGGCGCGGGCGAGGTGGAGGCCCAGGACTCTGACCCT
GCCCCTGCCTTCAGCAAGGCCCCCGGCAGCGCCGGCCACTACGAACTGCCGTGGGTTGAA
AAATATAGGCCAGTAAAGCTGAATGAAATTGTCGGGAATGAAGACACCGTGAGCAGGCTA
GAGGTCTTTGCAAGGGAAGGAAATGTGCCCAACATCATCATTGCGGGCCCTCCAGGAACC
GGCAAGACCACAAGCATTCTGTGCTTGGCCCGGGCCCTGCTGGGCCCAGCACTCAAAGAT
GCCATGTTGGAACTCAATGCTTCAAATGACAGCATGACCGACGGAGCCCAGCAAGCCTTG
AGGAGAACCATGGAAATCTACTCTAAAACCACTCGCTTCGCCCTTGCTTGTAATGCTTCG
GATAAGATCATCGAGCCCATTCAGTCCCGCTGTGCAGTCCTCCGGTACACAAAGCTGACC
GACGCCCAGATCCTCACCAGGCTGATGAATGTTATCGAGAAGGAGAGGGTACCCTACACT
GATGACGGCCTAGAAGCCATCATCTTCACGGCCCAGGGAGACATGAGGCAGGCGCTGAAC
AACCTGCAGTCCACCTTCTCAGGATTTGGCTTCATTAACAGTGAGAACGTGTTCAAGGTC
TGTGACGAGCCCCACCCACTGCTGGTAAAGGAGATGATCCAGCACTGTGTGAATGCCAAC
ATTGACGAAGCCTACAAGATTCTTGCTCACTTGTGGCATCTGGGCTACTCACCAGAAGAT
ATCATTGGCAACATCTTTCGAGTGTGTAAAACTTTCCAAATGGCAGAATACCTGAAACTG
GAGTTTATCAAGGAAATTGGATACACTCACATGAAAATAGCGGAAGGAGTGAACTCTCTT
TTGCAGATGGCAGGCCTCCTGGCAAGGCTGTGTCAGAAGACAATGGCCCCGGTGGCCAGT
TAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 968
Start on reference sequence 6
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : RFC2
Symbol Nomenclature : RFC2
SYNONYM : RFC40
Designation : A1 40 kDa subunit|RF-C 40 kDa subunit|activator 1 subunit 2|replication factor C subunit 2
Full Nomenclature : replication factor C (activator 1) 2, 40kDa
GENEID : 5982
GI : 60655816
GenBank Accession : AY889681
HGNC : 9970
MIM : 600404
Vega : OTTHUMG00000023239
Target GenBank: BC002813

 

Reference Sequence Alignment

  

HsCD00003489      ATGGAGGTGGAGGCCGTCTGTGGTGGCGCGGGCGAGGTGGAGGCCCAGGACTCTGACCCT
NM_002914.4       ATGGAGGTGGAGGCCGTCTGTGGTGGCGCGGGCGAGGTGGAGGCCCAGGACTCTGACCCT
NM_181471.2       ATGGAGGTGGAGGCCGTCTGTGGTGGCGCGGGCGAGGTGGAGGCCCAGGACTCTGACCCT
                  ************************************************************

HsCD00003489      GCCCCTGCCTTCAGCAAGGCCCCCGGCAGCGCCGGCCACTACGAACTGCCGTGGGTTGAA
NM_002914.4       GCCCCTGCCTTCAGCAAGGCCCCCGGCAGCGCCGGCCACTACGAACTGCCGTGGGTTGAA
NM_181471.2       GCCCCTGCCTTCAGCAAGGCCCCCGGCAGCGCCGGCCACTACGAACTGCCGTGGGTTGAA
                  ************************************************************

HsCD00003489      AAATATAGGCCAGTAAAGCTGAATGAAATTGTCGGGAATGAAGACACCGTGAGCAGGCTA
NM_002914.4       AAATATAGGCCAGTAAAGCTGAATGAAATTGTCGGGAATGAAGACACCGTGAGCAGGCTA
NM_181471.2       AAATATAGGCCAGTAAAGCTGAATGAAATTGTCGGGAATGAAGACACCGTGAGCAGGCTA
                  ************************************************************

HsCD00003489      GAGGTCTTTGCAAGGGAAGGAAATGTGCCCAACATCATCATTGCGGGCCCTCCAGGAACC
NM_002914.4       GAGGTCTTTGCAAGGGAAGGAAATGTGCCCAACATCATCATTGCGGGCCCTCCAGGAACC
NM_181471.2       GAGGTCTTTGCAAGGGAAGGAAATGTGCCCAACATCATCATTGCGGGCCCTCCAGGAACC
                  ************************************************************

HsCD00003489      GGCAAGACCACAAGCATTCTGTGCTTGGCCCGGGCCCTGCTGGGCCCAGCACTCAAAGAT
NM_002914.4       GGCAAGACCACAAGCATTCTGTGCTTGGCCCGGGCCCTGCTGGGCCCAGCACTCAAAGAT
NM_181471.2       GGCAAGACCACAAGCATTCTGTGCTTGGCCCGGGCCCTGCTGGGCCCAGCACTCAAAGAT
                  ************************************************************

HsCD00003489      GCCATGTTGGAACTCAATGCTTCAAATGACAG----------------------------
NM_002914.4       GCCATGTTGGAACTCAATGCTTCAAATGACAG----------------------------
NM_181471.2       GCCATGTTGGAACTCAATGCTTCAAATGACAGGGGCATTGACGTTGTGAGGAATAAAATT
                  ********************************                            

HsCD00003489      ------------------------------------------------------------
NM_002914.4       ------------------------------------------------------------
NM_181471.2       AAAATGTTTGCTCAACAAAAAGTCACTCTTCCCAAAGGCCGACATAAGATCATCATTCTG
                                                                              

HsCD00003489      --------------CATGACCGACGGAGCCCAGCAAGCCTTGAGGAGAACCATGGAAATC
NM_002914.4       --------------CATGACCGACGGAGCCCAGCAAGCCTTGAGGAGAACCATGGAAATC
NM_181471.2       GATGAAGCAGACAGCATGACCGACGGAGCCCAGCAAGCCTTGAGGAGAACCATGGAAATC
                                **********************************************

HsCD00003489      TACTCTAAAACCACTCGCTTCGCCCTTGCTTGTAATGCTTCGGATAAGATCATCGAGCCC
NM_002914.4       TACTCTAAAACCACTCGCTTCGCCCTTGCTTGTAATGCTTCGGATAAGATCATCGAGCCC
NM_181471.2       TACTCTAAAACCACTCGCTTCGCCCTTGCTTGTAATGCTTCGGATAAGATCATCGAGCCC
                  ************************************************************

HsCD00003489      ATTCAGTCCCGCTGTGCAGTCCTCCGGTACACAAAGCTGACCGACGCCCAGATCCTCACC
NM_002914.4       ATTCAGTCCCGCTGTGCAGTCCTCCGGTACACAAAGCTGACCGACGCCCAGATCCTCACC
NM_181471.2       ATTCAGTCCCGCTGTGCAGTCCTCCGGTACACAAAGCTGACCGACGCCCAGATCCTCACC
                  ************************************************************

HsCD00003489      AGGCTGATGAATGTTATCGAGAAGGAGAGGGTACCCTACACTGATGACGGCCTAGAAGCC
NM_002914.4       AGGCTGATGAATGTTATCGAGAAGGAGAGGGTACCCTACACTGATGACGGCCTAGAAGCC
NM_181471.2       AGGCTGATGAATGTTATCGAGAAGGAGAGGGTACCCTACACTGATGACGGCCTAGAAGCC
                  ************************************************************

HsCD00003489      ATCATCTTCACGGCCCAGGGAGACATGAGGCAGGCGCTGAACAACCTGCAGTCCACCTTC
NM_002914.4       ATCATCTTCACGGCCCAGGGAGACATGAGGCAGGCGCTGAACAACCTGCAGTCCACCTTC
NM_181471.2       ATCATCTTCACGGCCCAGGGAGACATGAGGCAGGCGCTGAACAACCTGCAGTCCACCTTC
                  ************************************************************

HsCD00003489      TCAGGATTTGGCTTCATTAACAGTGAGAACGTGTTCAAGGTCTGTGACGAGCCCCACCCA
NM_002914.4       TCAGGATTTGGCTTCATTAACAGTGAGAACGTGTTCAAGGTCTGTGACGAGCCCCACCCA
NM_181471.2       TCAGGATTTGGCTTCATTAACAGTGAGAACGTGTTCAAGGTCTGTGACGAGCCCCACCCA
                  ************************************************************

HsCD00003489      CTGCTGGTAAAGGAGATGATCCAGCACTGTGTGAATGCCAACATTGACGAAGCCTACAAG
NM_002914.4       CTGCTGGTAAAGGAGATGATCCAGCACTGTGTGAATGCCAACATTGACGAAGCCTACAAG
NM_181471.2       CTGCTGGTAAAGGAGATGATCCAGCACTGTGTGAATGCCAACATTGACGAAGCCTACAAG
                  ************************************************************

HsCD00003489      ATTCTTGCTCACTTGTGGCATCTGGGCTACTCACCAGAAGATATCATTGGCAACATCTTT
NM_002914.4       ATTCTTGCTCACTTGTGGCATCTGGGCTACTCACCAGAAGATATCATTGGCAACATCTTT
NM_181471.2       ATTCTTGCTCACTTGTGGCATCTGGGCTACTCACCAGAAGATATCATTGGCAACATCTTT
                  ************************************************************

HsCD00003489      CGAGTGTGTAAAACTTTCCAAATGGCAGAATACCTGAAACTGGAGTTTATCAAGGAAATT
NM_002914.4       CGAGTGTGTAAAACTTTCCAAATGGCAGAATACCTGAAACTGGAGTTTATCAAGGAAATT
NM_181471.2       CGAGTGTGTAAAACTTTCCAAATGGCAGAATACCTGAAACTGGAGTTTATCAAGGAAATT
                  ************************************************************

HsCD00003489      GGATACACTCACATGAAAATAGCGGAAGGAGTGAACTCTCTTTTGCAGATGGCAGGCCTC
NM_002914.4       GGATACACTCACATGAAAATAGCGGAAGGAGTGAACTCTCTTTTGCAGATGGCAGGCCTC
NM_181471.2       GGATACACTCACATGAAAATAGCGGAAGGAGTGAACTCTCTTTTGCAGATGGCAGGCCTC
                  ************************************************************

HsCD00003489      CTGGCAAGGCTGTGTCAGAAGACAATGGCCCCGGTGGCCAGTTAG
NM_002914.4       CTGGCAAGGCTGTGTCAGAAGACAATGGCCCCGGTGGCCAGTTAG
NM_181471.2       CTGGCAAGGCTGTGTCAGAAGACAATGGCCCCGGTGGCCAGTTAG
                  *********************************************

Pathways

NCI : ATR signaling pathway
NCI : Fanconi anemia pathway
Panther : DNA replication
Reactome : Activation of ATR in response to replication stress
Reactome : Cell Cycle
Reactome : Cell Cycle Checkpoints
Reactome : Cell Cycle, Mitotic
Reactome : Chromosome Maintenance
Reactome : DNA Repair
Reactome : DNA Replication
Reactome : DNA strand elongation
Reactome : Extension of Telomeres
Reactome : G2/M Checkpoints
Reactome : Gap-filling DNA repair synthesis and ligation in GG-NER
Reactome : Gap-filling DNA repair synthesis and ligation in TC-NER
Reactome : Global Genomic NER (GG-NER)
Reactome : Lagging Strand Synthesis
Reactome : Leading Strand Synthesis
Reactome : Nucleotide Excision Repair
Reactome : Polymerase switching
Reactome : Polymerase switching on the C-strand of the telomere
Reactome : Repair synthesis for gap-filling by DNA polymerase in TC-NER
Reactome : Repair synthesis of patch ~27-30 bases long by DNA polymerase
Reactome : S Phase
Reactome : Synthesis of DNA
Reactome : Telomere C-strand (Lagging Strand) Synthesis
Reactome : Telomere Maintenance
Reactome : Transcription-coupled NER (TC-NER)
WikiPathway : DNA Replication

Protein

UniProt : Q75MT5
UniProt : P35250
HPRD : 02675

Clone

Cloning Information : 12715
HIP Master Clone ID : 26914
Original Clone ID : FLH012715.01X

Organism


TAX_ID : 9606
Species Specific ID: 5982

Genome

Chromosome : 7
Map Location : 7q11.23
Ensembl : ENSG00000049541

Reagents

Labome : RFC2-antibody